Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion : liquid or solid

The study was funded by the Chinese Academy of Sciences Strategic Program (XDB13030100), the 1000 Talents program, and the National Natural Science Foundation of China (91649108) and a Wolfson merit award from the Royal Society all to J.R.S.; J.T was supported by the CAS-TWAS president's fellowship.Peer reviewedPublisher PD

Increased Variation in Body Weight and Food Intake Is Related to Increased Dietary Fat but Not Increased Carbohydrate or Protein in Mice

Funding This study was funded by the National Key R&D Program of China (2019YFA0801900) to JS and the Postdoctoral Innovation Fund (2021) to YW. The original diet exposure experiment was funded by the Chinese Academy of Sciences Strategic Program (XDB13030100). JS was also supported during this work by a PIFI professorial fellowship from CAS and a Wolfson merit award from the UK Royal Society. CORRECTION article Front. Nutr., 21 October 2022 Sec. Nutrition and Metabolism https://doi.org/10.3389/fnut.2022.1049766 Corrigendum: Increased variation in body weight and food intake is related to increased dietary fat but not increased carbohydrate or protein in micePeer reviewedPublisher PD

Effects of dietary macronutrients and body composition on glucose homeostasis in mice

Funding This work was supported by the Chinese Academy of Sciences Strategic Programs (XDA12030209 and XDB13030100), the 1000 Talents Program and a Wolfson Merit Award to J.R.SPeer reviewedPublisher PD

The hedonic overdrive model best explains high-fat diet-induced obesity in C57BL/6 mice

Open Access via the Wiley Agreement National Key R&D Program of China. Grant Number: 2019YFA0801900 Chinese Academy of Sciences. Grant Number: XDB13030100 National Science Foundation of China. Grant Number: 92057206Peer reviewe

Effects of dietary macronutrients on the hepatic transcriptome and serum metabolome in mice

ACKNOWLEDGMENTS The study was funded by National Key R&D Program of China (2019YFA0801900), National Postdoctoral Program for Innovative Talents (BX2021357), Postdoctoral Science Foundation (2021M693393) the Chinese Academy of Sciences Strategic Program (XDB13030100), the National Natural Science Foundation of China (91649108), a PIFI professorial fellowship from CAS and a Wolfson merit award from the UK Royal Society (all to J.R.S unless stated).Peer reviewedPublisher PD

Pseudonocardians A–C, New Diazaanthraquinone Derivatives from a Deap-Sea Actinomycete Pseudonocardia sp. SCSIO 01299

Pseudonocardians A–C (2–4), three new diazaanthraquinone derivatives, along with a previously synthesized compound deoxynyboquinone (1), were produced by the strain SCSIO 01299, a marine actinomycete member of the genus Pseudonocardia, isolated from deep-sea sediment of the South China Sea. The structures of compounds 1–4 were determined by mass spectrometry and NMR experiments (1H, 13C, HSQC, and HMBC). The structure of compound 1, which was obtained for the first time from a natural source, was confirmed by X-ray analysis. Compounds 1–3 exhibited potent cytotoxic activities against three tumor cell lines of SF-268, MCF-7 and NCI-H460 with IC50 values between 0.01 and 0.21 μm, and also showed antibacterial activities on Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212 and Bacillus thuringensis SCSIO BT01, with MIC values of 1–4 μg mL−1

Computational Method of Aging Index for Catalytic Converter Based on Wavelet Transform 1

Abstract: In order to control vehicle exhaust emission effectively, the conversion efficiency of catalytic converter is one of the key monitoring and evaluated indexes. The signal monitoring system of oxygen sensor is built by the TI2812 microcontroller. The method is to obtain the eigenvector and correlation coefficient of signal energy distribution, and to determine the two signal correlativity by using the Daubechies wavelet functions to analyze voltage signals of front and back oxygen sensors. The noise and vibration source are identified with the correlation coefficient. The aging level of catalytic converter is estimated according to the value of correlation coefficient. Experiments show that the method is more simple and effective than the traditional one. Copyright © 2013 IFSA

Effect of commercial slow-release urea product on in vitro rumen fermentation and ruminal microbial community using RUSITEC technique

Abstract Background The objectives of this study were to determine the effect of commercial slow-release urea (SRU) on in vitro fermentation characteristics, nutrient digestibility, gas production, microbial protein synthesis and bacterial community using a rumen simulation technique (RUSITEC). The experiment was a completely randomized design with four treatments and four replications of each treatment. Treatments were: control diet (no SRU addition), control diet plus 0.28% SRU (U28), or plus 0.56% SRU (U56), and control diet that was modified substituting a part of soybean meal equivalent to 0.35% SRU (MU35; dry matter [DM] basis). The experiment consisted of 8 d of adaptation and 7 d of data and sample collection. Rumen inoculum was obtained from three ruminally fistulated Angus cows fed the same diet to the substrate incubated. Results Digestibility of DM, organic matter (OM), crude protein (CP), fibre and starch was not affected, but daily production of gas (P < 0.07) and methane (P < 0.05) was quadratically increased with increasing SRU supplementation. The increase of SRU addition did not affect fermentation pH and total volatile fatty acid (VFA) production, whereas linearly (P < 0.01) decreased proportion of propionate, and linearly (P < 0.01) increased acetate to propionate ratio and ammonia nitrogen (N) concentration. The microbial N efficiency was also linearly (P < 0.03) improved with increasing supplementation of SRU. In comparison with control diet, the dietary substitution of SRU for part of soybean meal increased (P < 0.05) the digestibility of DM, OM and CP and decreased (P < 0.02) the total gas production. The total VFA production and acetate to propionate ratio did not differ between control and MU35, whereas the proportion of butyrate was lower (P < 0.05) and that of branched-chain VFA was greater (P < 0.05) with MU35 than control diet. Total and liquid-associated microbial N production as well as ammonia N concentration were greater (P < 0.03) with MU35 than control diet. Observed operational taxonomic units (OTUs), Shannon diversity index, and beta diversity of the microbial community did not differ among treatments. Taxonomic analysis revealed no effect of adding SRU on the relative abundance of bacteria at the phylum level, while at the genus level, the beneficial impact of SRU on relative abundance of Rikenellaceae and Prevotellaceae in feed particle-associated bacteria, and the abundance of Roseburia in liquid associate bacteria was greater (P < 0.05) with MU35. Conclusions Supplementation of a dairy cow diet with SRU showed potential of increase in ammonia N concentration and microbial protein production, and change fermentation pattern to more acetate production. Adding SRU in dairy cow diet also showed beneficial effect on improving digestibility of OM and fibre. The results suggest that SRU can partially substitute soybean meal in dairy cow diet to increase microbial protein production without impairing rumen fermentation

Efficient In Vitro TRAIL-Gene Delivery in Drug-Resistant A2780/DDP Ovarian Cancer Cell Line via Magnetofection

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) presents great promise as an anticancer agent for human cancer therapy. In this study, a magnetofection agent (polyMAG-l000) was evaluated for in vitro delivery of TRAIL gene towards drug-resistant A2780/DDP ovarian cancer cells. Transfection experiments showed that polyMAG-l000 was able to transfect A2780/DDP cells in vitro, leading to a higher level of TRAIL gene expression in the presence of a static magnetic field as compared to other transfection agent, such as Lipofectamine 2000. TRAIL gene expression in the A2780/DDP cells was also confirmed by Western blot analysis. Moreover, the TRAIL gene expression exhibited remarkable decrease in the cell viability, as determined by MTT assay. Importantly, PolyMAG-l000-mediated TRAIL gene transfection in the presence of anticancer drug cisplatin (CDDP) induced much higher percentages of apoptotic A2780/DDP cells, compared to TRAIL gene transfection or CDDP treatment alone. A further study by Western blot analysis indicated that cytochrome c release and caspase-9 cleavage pathway were associated with the initiation of the apoptosis in A2780/DDP cells. The results of this study indicate that polyMAG-l000 can be used as an efficient agent for TRAIL gene transfection in ovarian cancer cells

外泌体miR‐let‐7c‐5p通过白细胞介素10参与2型糖尿病患者的认知功能:一项横断面研究

Abstract Background Interleukin (IL)‐10 plays a notable role in the inflammatory‐associated mild cognitive impairment (MCI). We aimed to investigate whether IL‐10 and its upstream factors exert an impact on MCI in type 2 diabetes mellitus (T2DM) patients. Methods A total of 117 T2DM patients were recruited and divided into Control group and MCI group based on the presence or absence of MCI. Clinical parameters were collected. The Montreal Cognitive Assessment (MoCA) was conducted for global cognitive function. Digit Span Test (DST), Verbal Fluency Test (VFT), and Trail Making Test‐B (TMTB) were used to evaluate the executive functions of the diabetic patients. Trail Making Test‐A (TMTA) was performed to examine the information processing speed function. Patients' scene memory was examined by Logical Memory Test (LMT). After the baseline data were compared, correlation and regression analyses were performed to explore the relationship among IL‐10, miR‐let‐7c‐5p and cognitive function. Results Compared to 80 patients in the control group, 37 patients in the MCI group exhibited lower IL‐10 in plasma and higher miR‐let‐7c‐5p levels in exosomes from plasma. The IL‐10 level was negatively associated with MoCA. Likewise, miR‐let‐7c‐5p levels were negatively correlated with IL‐10 levels and MoCA. Elevated miR‐let‐7c‐5p levels and decreased IL‐10 levels are risk factors for MCI in T2DM patients. Increased miR‐let‐7c‐5p and downregulated IL‐10 may influence VFT and TMTB, respectively, associated with executive function. Conclusions We demonstrated that IL‐10 is correlated to the executive function of T2DM patients. Decreased IL‐10 may result from the regulation of miR‐let‐7c‐5p in exosomes