Correlation between Chlamydia Pneumoniae IgG Positive in Lung Cancer Patients and Cytokines Related to Radiation-induced Pulmonary Lesion

Background and objective There exsits intimate relationship between infection with chlamydia pneumoniae (Cpn) and lung cancer incidence. But few studies have been reported about radiation-induced pulmonary lesion in lung cancer patients infected with Cpn. The aim of this study is to explore the correlation between cytokines related to radiation-induced pulmonary lesion and Cpn IgG positive in lung cancer patients. Methods A total of 69 patients with lung cancer received chest radiotherapy. Blood samples were collected and frozen before radiotherapy (pre-RT), middle radiotherapy (mid-RT) and after radiotherapy (post-RT). Cpn IgG and levels of IL-1β, SP-A, TGF-β, and TNF-α were measured by enzymelinked immunosorbent assay (ELISA). Results In the total of 69 patients, 21 patients were Cpn IgG positive, 48 patients negative. The positive rate was 30.43%. In mid-RT concentration of IL-1β in Cpn IgG positive and negative group were (35.82±10.09) ng/L and (30.01±6.46) ng/L, with statistically significant difference (P < 0.05). Pre-RT and post-RT concentrations of IL-1β in Cpn IgG positive and negative group had no statistically significant difference. Mid-RT concentrations of SP-A in Cpn IgG positive group and negative group were (641.78±106.81) ng/L and (100.86±61.4) ng/L respectively, with statistically significant difference (P < 0.05). Post-RT concentration of SP-A in Cpn IgG positive and negative group were (657.47±115.19) ng/L and (93.23±47.15) ng/L respectively, with statistically significant difference (P < 0.05). Concentrations of TNF-α in Cpn IgG positive and negative group had no statistically significant difference. Concentrations of TGF-β in Cpn IgG positive group were (710.67±358.16) pg/mL in pre-RT, (1,002.06±542.16) pg/mL in mid-RT, (2,125.16±1,522.29) pg/mL in post-RT; those in negative group were (867.77±412.48) pg/mL, (914.05±425.70) pg/mL, (1,073.36±896.01) pg/mL. Concentration of TGF-β in post-RT between Cpn IgG positive and negative group had statistically significant difference (P < 0.05). Conclusion Cpn IgG positive in lung cancer patients influenced levels of IL-1β, SP-A, TGF-β during chest radiotherapy. This might aggravate radiation-induced pulmonary lesion

Effect of DBP inhibition on vascular calcification in chronic uremic rats

Objective To evaluate the effect of D-site binding protein （DBP） inhibition on vascular calcification in chronic uremic rats. Methods GSE146638 was downloaded from the public database （Gene Expression Omnibus， GEO）， and a total of 10 samples from rat of mRNA expression profiles were included in this study. DESeq2 was used to analyze the differentially expressed genes in the aortic smooth muscle between the control and chronic uremic rat model groups. RT-qPCR was employed to detect the expression levels of SLC22A2， ATF3， DBP and SMPD3. Male SD rats were randomly divided into four groups. In the blank group， no treatment was given. In the model group， chronic uremic rat models were established by nephrectomy of 2/3 of bilateral kidneys， and the surviving rats were fed with 8-week high phosphorus diet to induce vascular calcification. In the negative control lentivirus group （shNC group）， the rats were injected with negative control lentivirus for 4 weeks after establishing the vascular calcification uremic rat models. In the small interfering DBP group （shDBP group）， the rats were treated with siDBP lentivirus for 4 weeks after the establishment of vascular calcification uremic rat models. After corresponding interventions， the rat weight was measured， and serum creatinine， serum phosphorus， serum calcium and urea nitrogen levels were detected among four groups. Immunohistochemical staining and HE staining of the thoracic aorta tissues were used to evaluate the degree of injury. RT-qPCR and western blot were adopted to quantitatively measure the relative expression levels of Runt-related transcription factor 2 （RUNX2） and DBP. Results Compared with the control group， the rat weight was significantly less， whereas the serum creatinine levels were significantly higher in the model， shNC and shDBP groups （all P < 0.05）. In the model group， the thoracic aortic vascular wall was thickened， and the expression levels of RUNX2 and proliferating cell nuclear antigen （PCNA） were up-regulated. Compared with the model group， the serum creatinine level was declined， the expression levels of RUNX2 mRNA and protein in the thoracic aorta were down-regulated （all P < 0.05）， the expression level of PCNA in the thoracic aorta was down-regulated and the thoracic aortic vascular wall thickening was mitigated. Conclusion Inhibition of DBP may suppress vascular calcification and alleviate uremia-associated symptoms in chronic uremic rat models by down-regulating the expression levels of RUNX2 mRNA and protein in the aorta

Glassy carbon electrode modified with 7,7,8,8-tetracyanoquinodimethane and graphene oxide triggered a synergistic effect: low-potential amperometric detection of reduced glutathione.

A sensitive electrochemical sensor based on the synergistic effect of 7,7,8,8-tetracyanoquinodimethane (TCNQ) and graphene oxide (GO) for low-potential amperometric detection of reduced glutathione (GSH) in pH 7.2 phosphate buffer solution (PBS) has been reported. This is the first time that the combination of GO and TCNQ have been successfully employed to construct an electrochemical sensor for the detection of glutathione. The surface of the glassy carbon electrode (GCE) was modified by a drop casting using TCNQ and GO. Cyclic voltammetric measurements showed that TCNQ and GO triggered a synergistic effect and exhibited an unexpected electrocatalytic activity towards GSH oxidation, compared to GCE modified with only GO, TCNQ or TCNQ/electrochemically reduced GO. Three oxidation waves for GSH were found at −0.05, 0.1 and 0.5 V, respectively. Amperometric techniques were employed to detect GSH sensitively using a GCE modified with TCNQ/GO at −0.05 V. The electrochemical sensor showed a wide linear range from 0.25 to 124.3 μM and 124.3 μM to 1.67 mM with a limit of detection of 0.15 μM. The electroanalytical sensor was successfully applied towards the detection of GSH in an eye drop solution

Morphology tailoring and temperature sensitivity control of waist cross-linked micelles and evaluation of their application as intelligent drug carriers

A novel type of waist cross-linked micelle (WCM) was developed as an intelligent drug carrier via the self-assembly guiding free radical polymerization of an amphiphilic oligomer: octadecyl, polyethylene glycol butenedioates (O-B-EGs). By changing the concentration of O-B-EG reaction solution, WCMs with monolayer, compound and vesicle-like morphologies were obtained. These WCMs showed controllable temperature responsive properties. DLS and UV-vis analyses indicate that the critical temperatures at which WCMs show an abrupt change in particle size evidently increases with the increase in the molecular weight of the PEG chains. Direct switching of the release of pyrene in WCMs is also realized by a slight change of temperature. Pyrene is released rapidly at the temperatures around the critical temperature of the WCMs, but a further increase in temperature shuts down the release of pyrene. More importantly, these WCMs exhibit reversible and rapid pyrene releasing-absorbing behavior. We suggest that these excellent properties endow WCMs with great potential in drug encapsulation and controlled releaseNational Natural Science Foundation of China [50873082, 30700020]; Research Fund for the Doctoral Program of Higher Education [20070384047]; Scientific and Technical Project of Fujian Province of China [2009J1009

A Physically Based Spatial Expansion Algorithm for Surface Air Temperature and Humidity

An algorithm was developed to expand the surface air temperature and air humidity to a larger spatial domain, based on the fact that the variation of surface air temperature and air humidity is controlled jointly by the local turbulence and the horizontal advection. This study proposed an algorithm which considers the advective driving force outside the thermal balance system and the turbulent driving force and radiant driving force inside the thermal balance system. The surface air temperature is determined by a combination of the surface observations and the regional land surface temperature observed from a satellite. The average absolute difference of the algorithm is 0.65 degree and 0.31 mb, respectively, for surface air temperature and humidity expansion, which provides a promising approach to downscale the two surface meteorological variables

The Problems of Civil Law in China and Japan

千葉大学大学院人文社会科学研究科研究プロジェクト報告書第171集『中日における民法現代化の課題』 小賀野晶一

Prognostic value and immune-infiltration pattern of FOXD3-AS1 in patients with glioma

Gliomas are difficult-to-treat brain tumors due to their aggressive nature, rapid proliferation, and high invasiveness (Zhang et al., J Cell Biochem, 2019, 120 (9), 15106–15118; Ge et al., Int J Biochem Cell Biol, 2021, 139, 106054). FOXD3-AS1 has been identified as an emerging potential target for tumor prediction and treatment in many studies (Qin et al., Front Oncol, 2021, 11, 688027). However, the utility of FOXD3-AS1 has not been reported in glioma patients (Li et al., Cancer Manag Res, 2021, 13, 9037–9048). The differential profiles of FOXD3-AS1 in TCGA–GBMLGG database were analyzed across clinical subgroups. The analysis of overall survival (OS), disease-specific survival (DSS), and progression-free interval (PFI) revealed that a high level of FOXD3-AS1 was associated with a poor prognosis and survival outcome. Based on the Cox regression analysis, FOXD3-AS1 was found to be a high-risk factor for glioma that affects prognosis outcomes independently. More importantly, because oxidative stress is closely linked to glioma prognosis, we focused on the potential mechanisms of six oxidative stress co-expressed genes with FOXD3-AS1. In addition, the predictive value of FOXD3-AS1 was determined for each clinical subgroup status. The ROC curve results showed that FOXD3-AS1 had a good predictive performance. A stratified clinicopathological subgroup analysis revealed that high expression of FOXD3-AS1 is associated with a poor prognosis. This also indicates a link between FOXD3-AS1 and tumorigenesis and prognosis, which has potential application value. Furthermore, the immune cell infiltration of FOXD3-AS1 and the signal marker correlation suggested that immune cell infiltration differed significantly between immune cell subsets. To the best of our knowledge, this is the first report to investigate FOXD3-AS1 in glioma and how it may modulate GBM and LGG immune microenvironments. Furthermore, FOXD3-AS1 was detected in tumor and paraneoplastic tissues using RT–qPCR. Transwell analysis verified the migration and invasion of the FOXD3-AS1 knockout group in vitro to a certain extent. In conclusion, FOXD3-AS1 can be used as a prognostic indicator for GBM and LGG, and it is closely related to immune infiltration and response to oxidative stress, which may contribute to the advancement of glioma immunotherapy research

Bisphenol A and 17β-Estradiol Promote Arrhythmia in the Female Heart via Alteration of Calcium Handling

There is wide-spread human exposure to bisphenol A (BPA), a ubiquitous estrogenic endocrine disruptor that has been implicated as having potentially harmful effects on human heart health. Higher urine BPA concentrations have been shown to be associated with cardiovascular diseases in humans. However, neither the nature nor the mechanism(s) of BPA action on the heart are understood. leak suppressed estrogen-induced triggered activities. The rapid response of female myocytes to estrogens was abolished in an estrogen receptor (ER) β knockout mouse model. leak. Our study provides the first experimental evidence suggesting that exposure to estrogenic endocrine disrupting chemicals and the unique sensitivity of female hearts to estrogens may play a role in arrhythmogenesis in the female heart