Recruitment, augmentation and apoptosis of rat osteoclasts in 1,25-(OH)2D3 response to short-term treatment with 1,25-dihydroxyvitamin D3in vivo

Background Although much is known about the regulation of osteoclast (OC) formation and activity, little is known about OC senescence. In particular, the fate of of OC seen after 1,25-(OH)2D3 administration in vivo is unclear. There is evidence that the normal fate of OC is to undergo apoptosis (programmed cell death). We have investigated the effect of short-term application of high dose 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on OC apoptosis in an experimental rat model. Methods OC recruitment, augmentation and apoptosis was visualised and quantitated by staining histochemically for tartrate resistant acid phosphatase (TRAP), double staining for TRAP/ED1 or TRAP/DAPI, in situ DNA fragmentation end labelling and histomorphometric analysis. Results Short-term treatment with high-dose 1,25-(OH)2D3 increased the recruitment of OC precursors in the bone marrow resulting in a short-lived increase in OC numbers. This was rapidly followed by an increase in the number of apoptotic OC and their subsequent removal. The response of OC to 1,25-(OH)2D3 treatment was dose and site dependent; higher doses producing stronger, more rapid responses and the response in the tibiae being consistently stronger and more rapid than in the vertebrae. Conclusions This study demonstrates that (1) after recruitment, OC are removed from the resorption site by apoptosis (2) the combined use of TRAP and ED1 can be used to identify OC and their precursors in vivo (3) double staining for TRAP and DAPI or in situ DNA fragmentation end labelling can be used to identify apoptotic OC in vivo

Garvey-Kelson Relations for Nuclear Charge Radii

The Garvey-Kelson relations (GKRs) are algebraic expressions originally developed to predict nuclear masses. In this letter we show that the GKRs provide a fruitful framework for the prediction of other physical observables that also display a slowly-varying dynamics. Based on this concept, we extend the GKRs to the study of nuclear charge radii. The GKRs are tested on 455 out of the approximately 800 nuclei whose charge radius is experimentally known. We find a rms deviation between the GK predictions and the experimental values of only 0.01 fm. This should be contrasted against some of the most successful microscopic models that yield rms deviations almost three times as large. Predictions - with reliable uncertainties - are provided for 116 nuclei whose charge radius is presently unknown.Comment: 4 pages and 3 figure

Threshold Electrodisintegration of ^3He

Cross sections were measured for the near-threshold electrodisintegration of ^3He at momentum transfer values of q=2.4, 4.4, and 4.7 fm^{-1}. From these and prior measurements the transverse and longitudinal response functions R_T and R_L were deduced. Comparisons are made against previously published and new non-relativistic A=3 calculations using the best available NN potentials. In general, for q<2 fm^{-1} these calculations accurately predict the threshold electrodisintegration of ^3He. Agreement at increasing q demands consideration of two-body terms, but discrepancies still appear at the highest momentum transfers probed, perhaps due to the neglect of relativistic dynamics, or to the underestimation of high-momentum wave-function components.Comment: 9 pages, 7 figures, 1 table, REVTEX4, submitted to Physical Review

Analyzing powers Ayy, Axx, Axz and Ay in the dd->3Hen reaction at 270 MeV

The data on the tensor Ayy, Axx, Axz and vector Ay analyzing powers in the dd->3Hen obtained at Td= 270 MeV in the angular range 0 - 110 degrees in the c.m. are presented. The observed negative sign of the tensor analyzing powers Ayy, Axx and Axz at small angles clearly demonstrate the sensitivity to the ratio of the D and S wave component of the 3He wave function. However, the one-nucleon exchange calculations by using the standard 3He wave functions have failed to reproduce the strong variation of the tensor analyzing powers as a function of the angle in the c.m.Comment: 8 pages, 7 figures, 4 tables. Submitted to EPJ

Examining exotic structure of proton-rich nucleus 23^{23}Al

The longitudinal momentum distribution (P_{//}) of fragments after one-proton removal from ^{23} Al and reaction cross sections (\sigma_R) for ^{23,24} Al on carbon target at 74A MeV have been measured. The ^{23,24} Al ions were produced through projectile fragmentation of 135 A MeV ^{28} Si primary beam using RIPS fragment separator at RIKEN. P_{//} is measured by a direct time-of-flight (TOF) technique, while \sigma_R is determined using a transmission method. An enhancement in \sigma_R is observed for ^{23} Al compared with ^{24} Al. The P_{//} for ^{22} Mg fragments from ^{23} Al breakup has been obtained for the first time. FWHM of the distributions has been determined to be 232 \pm 28 MeV/c. The experimental data are discussed by using Few-Body Glauber model. Analysis of P_{//} demonstrates a dominant d-wave configuration for the valence proton in ground state of ^{23} Al, indicating that ^{23} Al is not a proton halo nucleus

Relativistic effects and two-body currents in 2H(e⃗,e′p)n^{2}H(\vec{e},e^{\prime}p)n using out-of-plane detection

Measurements of the ${^2}H(\vec{e},e^{\prime}p)n$ reaction were performed using an 800-MeV polarized electron beam at the MIT-Bates Linear Accelerator and with the out-of-plane magnetic spectrometers (OOPS). The longitudinal-transverse, $f_{LT}$ and $f_{LT}^{\prime}$, and the transverse-transverse, $f_{TT}$, interference responses at a missing momentum of 210 MeV/c were simultaneously extracted in the dip region at Q$^2$=0.15 (GeV/c)$^2$. On comparison to models of deuteron electrodisintegration, the data clearly reveal strong effects of relativity and final-state interactions, and the importance of the two-body meson-exchange currents and isobar configurations. We demonstrate that these effects can be disentangled and studied by extracting the interference response functions using the novel out-of-plane technique.Comment: 4 pages, 4 figures, and submitted to PRL for publicatio

Self energies of the pion and the delta isobar from the ^3He(e,e'pi^+)^3H reaction

In a kinematically complete experiment at the Mainz microtron MAMI, pion angular distributions of the $^3$He(e,e'$\pi^+)^3$H reaction have been measured in the excitation region of the $\Delta$ resonance to determine the longitudinal ($L$), transverse ($T$), and the $LT$ interference part of the differential cross section. The data are described only after introducing self-energy modifications of the pion and $\Delta$-isobar propagators. Using Chiral Perturbation Theory (ChPT) to extrapolate the pion self energy as inferred from the measurement on the mass shell, we deduce a reduction of the $\pi^+$ mass of $\Delta m_{\pi^+} = (-1.7^{+ 1.7}_{- 2.1})$ MeV/c$^2$ in the neutron-rich nuclear medium at a density of $\rho = (0.057^{+ 0.085}_{- 0.057})$ fm$^{-3}$. Our data are consistent with the $\Delta$ self energy determined from measurements of $\pi^0$ photoproduction from $^4$He and heavier nuclei.Comment: Elsart, 12 pages and 4 figures, Correspondent: Professor Dr. Dr. h.c. mult. Achim Richter, [email protected], submitted to Phys. Rev. Let

Relativistic cosmological perturbation scheme on a general background: scalar perturbations for irrotational dust

In standard perturbation approaches and N-body simulations, inhomogeneities are described to evolve on a predefined background cosmology, commonly taken as the homogeneous-isotropic solutions of Einstein's field equations (Friedmann-Lema\^itre-Robertson-Walker (FLRW) cosmologies). In order to make physical sense, this background cosmology must provide a reasonable description of the effective, i.e. spatially averaged, evolution of structure inhomogeneities also in the nonlinear regime. Guided by the insights that (i) the average over an inhomogeneous distribution of matter and geometry is in general not given by a homogeneous solution of general relativity, and that (ii) the class of FLRW cosmologies is not only locally but also globally gravitationally unstable in relevant cases, we here develop a perturbation approach that describes the evolution of inhomogeneities on a general background being defined by the spatially averaged evolution equations. This physical background interacts with the formation of structures. We derive and discuss the resulting perturbation scheme for the matter model `irrotational dust' in the Lagrangian picture, restricting our attention to scalar perturbations.Comment: 18 pages. Matches published version in CQ