Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence

Prohibitin 1 (PHB1) is a highly conserved protein that is mainly localized to the inner mitochondrial membrane and has been implicated in regulating mitochondrial function in yeast. Because mitochondria are emerging as an important regulator of vascular homeostasis, we examined PHB1 function in endothelial cells. PHB1 is highly expressed in the vascular system and knockdown of PHB1 in endothelial cells increases mitochondrial production of reactive oxygen species via inhibition of complex I, which results in cellular senescence. As a direct consequence, both Akt and Rac1 are hyperactivated, leading to cytoskeletal rearrangements and decreased endothelial cell motility, e.g., migration and tube formation. This is also reflected in an in vivo angiogenesis assay, where silencing of PHB1 blocks the formation of functional blood vessels. Collectively, our results provide evidence that PHB1 is important for mitochondrial function and prevents reactive oxygen species–induced senescence and thereby maintains the angiogenic capacity of endothelial cells

Abordaje de enfermería en el cuidado de niños con trastorno del espectro autista, desde la teoría del cuidado humanizado y la teoría: interacción para la evaluación de la salud infantil

Introduction: Autism spectrum disorders (ASD) are neuropsychiatric problems whose first manifestations appear before three years of age and persist throughout life. Objective: To recognize the nursing approach in the care of children with autism spectrum disorder, from the theory of humanized care and the theory: interaction for the evaluation of child health. Materials and method: This study is a systematic review, through the search equation in DeCS with the keywords: Autism, nursing, humanized care, in different databases. Results: Through Watson's theory, the nursing approach documented through the concepts: nurse/patient interaction, phenomenological field, transpersonal care relationship, moment of care, humanization can be fulfilled in the care of autistic children and their families. Also from Barnard's theory with the integration of theoretical elements: clarity of the child's messages, child's response to the caregiver, parental sensitivity to the child's signals, allowing work on the child's health of the child with ASD. Conclusion: Behavioral approaches produce positive results in children with ASD.Introducción: Los trastornos del espectro autista (TEA), son problemas neuropsiquiátricos cuyas primeras manifestaciones aparecen antes de los tres años de edad y persisten durante toda la vida. Objetivo: Reconocer el abordaje de enfermería en el cuidado de niños con trastorno del espectro autista, desde la teoría del cuidado humanizado y la teoría: interacción para la evaluación de la salud infantil. Materiales y método: El presente estudio es una revisión sistemática, por medio de la ecuación de búsqueda en DeCS con las palabras clave: Autismo, enfermería, cuidado humanizado, en diferentes bases de datos. Resultados: A través de la teoría de Watson el abordaje enfermero documentado a traves de los conceptos: interacción enfermera/paciente, campo fenomenológico, relación cuidado transpersonal, momento de cuidado, se puede dar cumplimiento a la humanización en la atención de niños autistas y sus familias. Asimismo desde la teoría de Barnard con la integración de los elementos teóricos: claridad de los mensajes del niño, respuesta del niño al cuidador, sensibilidad de los padres a las señales del hijo, permitiendo trabajar en la salud infantil del niño con TEA. Conclusión: Los acercamientos conductuales producen resultados positivos en niños con TEA

Abordaje de contextos desde los enfoques narrativos.

El presente documento se ha elaborado con el objetivo de realizar el análisis de los relatos de los casos de violencia y esperanza contenidos en el diplomado de profundización acompañamiento psicosocial en escenarios de violencia de la Universidad Nacional Abierta y a Distancia UNAD, mediante la lectura crítica y reflexiva que permitió comprender y dar inicio al abordaje de los relatos, los cuales brindaron herramientas de apoyo para lograr identificar la temática a realizar, su abordaje contribuyo significativamente para el desarrollo y aprendizaje de conocimientos previos, igualmente se seleccionó uno de los relatos de vida tomados del libro Voces: historias de violencia y esperanza en Colombia. Denominado: Relato 1. Jennifer Pinzón, para luego responder a dichas preguntas las cuales llevaban al estudiante al análisis reflexivo frente a las vivencias obtenidas tanto físicas, psicológicas y emocionales por la violencia de la época por parte de Jennifer pinzón. En ese mismo sentido, se resuelven preguntas orientadoras presentes en la guía de actividades, con el fin de brindar estrategias que contribuyeran a proponer y elaborar grupalmente interrogantes que se clasifican de la siguiente manera: circulares, reflexivas y estratégicas, cuya finalidad es generar una mayor comprensión del caso elegido. Por otra parte, también se realizó el análisis del caso Pandurí y se inició la propuesta de abordaje psicosocial, donde se permite proponer estrategias que nos ha brinda el presente diplomado, posteriormente se resolvieron interrogantes con el ánimo de generar parámetros fundamentales para fijar bases de la propuesta psicosocial del caso antes mencionado y esta a su vez ayudaron a generar resultados positivos. No obstante, teniendo claro el reflejo de una violencia vivida bajo el flagelo del horror, se expone el informe de la experiencia de ejercicio de la foto voz, donde queda plasmado las vivencias obtenidas de manera individual y grupal, su aporte logro suscitar la reflexión y desarrollar aspectos fundamentales que llevaron no solo al análisis visual sino ir más allá de la memoria histórica donde se guardan el pasado sin importar el daño que ha causado en nuestro país. Por último se encuentra el link colaborativo del blog y las referencias bibliográficas.The present document has been elaborated with the objective of carrying out the analysis of the stories of the cases of violence and hope contained in the diploma of deepening psychosocial accompaniment in scenarios of violence of the National Open University and Distance UNAD, through critical and reflexive reading that allowed us to understand and initiate the approach of the stories, which provided support tools to identify the theme to be carried out, its approach contributed significantly to the development and learning of previous knowledge, and one of the life stories taken from the Voices book: stories of violence and hope in Colombia. Denominated: Story 1. Jennifer Pinzón, to then respond to these questions which led the student to reflective analysis of the experiences obtained both physical, psychological and emotional violence of the time by Jennifer Chaffinch. In this same sense, orienting questions present in the activity guide are solved, in order to provide strategies that contribute to propose and elaborate group questions that are classified as follows: circular, reflexive and strategic, whose purpose is to generate a greater understanding of the chosen case. On the other hand, the analysis of the Pandurí case was also carried out and the psychosocial approach proposal was initiated, where it is possible to propose strategies that the present diploma has given us, later questions were resolved in order to generate fundamental parameters to establish bases of the psychosocial proposal of the aforementioned case and this in turn helped to generate positive results. However, having a clear reflection of a violence lived under the scourge of horror, the report of the experience of photo voice exercise is exposed, where the experiences obtained in an individual and group manner are captured, their contribution is able to provoke reflection and develop fundamental aspects that led not only to visual analysis but to go beyond the historical memory where the past is kept regardless of the damage it has caused in our country. Finally, there is the collaborative link of the blog and the bibliographical references

Genetic deficiency or pharmacological inhibition of miR-33 protects from kidney fibrosis

Previous work has reported the important links between cellular bioenergetics and the development of chronic kidney disease, highlighting the potential for targeting metabolic functions to regulate disease progression. More recently, it has been shown that alterations in fatty acid oxidation (FAO) can have an important impact on the progression of kidney disease. In this work, we demonstrate that loss of miR-33, an important regulator of lipid metabolism, can partially prevent the repression of FAO in fibrotic kidneys and reduce lipid accumulation. These changes were associated with a dramatic reduction in the extent of fibrosis induced in 2 mouse models of kidney disease. These effects were not related to changes in circulating leukocytes because bone marrow transplants from miR-33–deficient animals did not have a similar impact on disease progression. Most important, targeted delivery of miR-33 peptide nucleic acid inhibitors to the kidney and other acidic microenvironments was accomplished using pH low insertion peptides as a carrier. This was effective at both increasing the expression of factors involved in FAO and reducing the development of fibrosis. Together, these findings suggest that miR-33 may be an attractive therapeutic target for the treatment of chronic kidney disease

Global variations in diabetes mellitus based on fasting glucose and haemogloblin A1c

Fasting plasma glucose (FPG) and haemoglobin A1c (HbA1c) are both used to diagnose diabetes, but may identify different people as having diabetes. We used data from 117 population-based studies and quantified, in different world regions, the prevalence of diagnosed diabetes, and whether those who were previously undiagnosed and detected as having diabetes in survey screening had elevated FPG, HbA1c, or both. We developed prediction equations for estimating the probability that a person without previously diagnosed diabetes, and at a specific level of FPG, had elevated HbA1c, and vice versa. The age-standardised proportion of diabetes that was previously undiagnosed, and detected in survey screening, ranged from 30% in the high-income western region to 66% in south Asia. Among those with screen-detected diabetes with either test, the agestandardised proportion who had elevated levels of both FPG and HbA1c was 29-39% across regions; the remainder had discordant elevation of FPG or HbA1c. In most low- and middle-income regions, isolated elevated HbA1c more common than isolated elevated FPG. In these regions, the use of FPG alone may delay diabetes diagnosis and underestimate diabetes prevalence. Our prediction equations help allocate finite resources for measuring HbA1c to reduce the global gap in diabetes diagnosis and surveillance.peer-reviewe

Intervención del trabajador social para el fortalecimiento de las estrategias metodológicas con grupo juveniles beneficiarios de los proyectos de desarrollados en el secretariado de pastoral social de la ciudad de Cartagena de indias. Año 2006

Reflexión teórica, metodológico y epistemológica sobre el proceso de intervención frente a la articulación teórica práctica, así como el de los aprendizajes adquiridos en cuanto al desarrollo de destrezas, habilidades, competencias y capacidades en la formación integral del estudiante.PregradoTrabajador(a) Socia

Intervención del trabajador social para el fortalecimiento de las estrategias metodológicas con grupo juveniles beneficiarios de los proyectos de desarrollados en el secretariado de pastoral social de la ciudad de Cartagena de indias. Año 2006

Tesis (Trabajadora Social) -- Universidad de Cartagena. Facultad de Ciencias Sociales y Educación. Programa de Trabajo Social, 2006Reflexión teórica, metodológica y epistemológica sobre el proceso de intervención frente a la articulación teoría práctica, como aprendizajes adquiridos, destrezas, habilidades, competencias y capacidades en la formación del estudiante

Gene Expression Signature in Patients with Symptomatic Peripheral Artery Disease

Objective: Peripheral artery disease (PAD) is an atherothrombotic disease of the lower limbs with substantial morbidity and mortality. We used next-generation sequencing to identify genome-wide expression signatures associated with prevalent PAD and its outcomes. Approach and Results: We performed whole blood RNA sequencing among patients with severe symptomatic PAD undergoing lower extremity revascularization and controls. Dysregulated pathways and blood transcriptional modules were identified by comparing patients with PAD (n=42) to age-and sex-matched controls (N=29). The identified signature was compared in patients with PAD before LER with or without incident major adverse cardiac or limb events (MACLE). A novel microRNA associated with prevalent PAD and incident MACLE was then evaluated in a mouse hindlimb ischemia model. One hundred twenty-seven transcripts were differentially expressed (77 upregulated and 50 downregulated; adjusted P\u3c0.05, |log2foldchange| \u3e0.5) and analyzed using weighted gene co-expression network analysis. Weighted gene co-expression network analysis revealed blood modules enriched for immune activation, secretory granules, and coagulation in patients with PAD. Of these 127 differentially expressed transcripts, 40 were significantly associated with MACLE (log-rank false discovery rate \u3c0.1). MicroRNA-4477b was significantly increased in patients with PAD with subsequent MACLE and in a mouse hindlimb ischemia model. Conclusions: A whole blood transcript signature identified patients with symptomatic PAD and PAD patients at increased risk of MACLE. A previously uncharacterized transcript microRNA-4477b was overexpressed in prevalent PAD, incident MACLE, and in a mouse hindlimb ischemia model. Our novel transcriptomic signature provides insight into potential mechanisms of patients with severe symptomatic PAD

General Labor Well-Being in Latin American Dentists during the COVID-19 Pandemic

Abstract: This study aimed to determine the general labor well-being of Latin American dentists according to sociodemographic characteristics during the COVID-19 pandemic. A cross-sectional study was conducted in a final sample of 2214 participants from 11 countries. A validated online questionnaire on general work well-being was used (data collection period from 1 June to 10 July 2021), containing two dimensions: psychosocial well-being and collateral effects. The sociodemographic characteristics of the dentists and their perception of the economic impact of the pandemic were also recorded. A multivariate linear regression analysis was performed (hierarchical regression model) to evaluate the joint effect of the explanatory variables on labor well-being and the changes in the variance between each model. A score of psychosocial well-being of 233.6 + 40.2 and collateral effects of 45 + 20.1 was found. Psychosocial well-being was associated with sex, country of origin, academic training achieved, type of dental activity, and perceived impact during the pandemic (p < 0.05). Somatization was frequently manifested through back pain (88.2%) and muscular tensions (87.2%). Women, those who worked 41 or more hours and had between 1 to 15 years of professional experience presented azgreater collateral effect (p < 0.001). The impact of the COVID-19 pandemic a year and a half after it began on the labor well-being of Latin American dentists was evidenced with important interactions with social characteristics