4 research outputs found
The Red Fox Y-Chromosome in Comparative Context
While the number of mammalian genome assemblies has proliferated, Y-chromosome
assemblies have lagged behind. This discrepancy is caused by biological features of the Y-chromosome,
such as its high repeat content, that present challenges to assembly with short-read, next-generation
sequencing technologies. Partial Y-chromosome assemblies have been developed for the cat (Felis catus),
dog (Canis lupus familiaris), and grey wolf (Canis lupus lupus), providing the opportunity to examine
the red fox (Vulpes vulpes) Y-chromosome in the context of closely related species. Here we present a
data-driven approach to identifying Y-chromosome sequence among the scaffolds that comprise the
short-read assembled red fox genome. First, scaffolds containing genes found on the Y-chromosomes
of cats, dogs, and wolves were identified. Next, analysis of the resequenced genomes of 15 male
and 15 female foxes revealed scaffolds containing male-specific k-mers and patterns of inter-sex
copy number variation consistent with the heterogametic chromosome. Analyzing variation across
these two metrics revealed 171 scaffolds containing 3.37 Mbp of putative Y-chromosome sequence.
The gene content of these scaffolds is consistent overall with that of the Y-chromosome in other
carnivore species, though the red fox Y-chromosome carries more copies of BCORY2 and UBE1Y than
has been reported in related species and fewer copies of SRY than in other canids. The assignment of
these scaffolds to the Y-chromosome serves to further characterize the content of the red fox draft
genome while providing resources for future analyses of canid Y-chromosome evolution
p21 produces a bioactive secretome that places stressed cells under immunosurveillance
Immune cells identify and destroy damaged cells to prevent them from causing cancer or other pathologies by mechanisms that remain poorly understood. Here, we report that the cell-cycle inhibitor p21 places cells under immunosurveillance to establish a biological timer mechanism that controls cell fate. p21 activates retinoblastoma protein (Rb)-dependent transcription at select gene promoters to generate a complex bioactive secretome, termed p21-activated secretory phenotype (PASP). The PASP includes the chemokine CXCL14, which promptly attracts macrophages. These macrophages disengage if cells normalize p21 within 4 days, but if p21 induction persists, they polarize toward an M1 phenotype and lymphocytes mount a cytotoxic T cell response to eliminate target cells, including preneoplastic cells. Thus, p21 concurrently induces proliferative arrest and immunosurveillance of cells under duress
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Y-Chromosome Markers for the Red Fox
The de novo assembly of the red fox (Vulpes vulpes) genome has facilitated the development of genomic tools for the species. Efforts to identify the population history of red foxes in North America have previously been limited by a lack of information about the red fox Y-chromosome sequence. However, a megabase of red fox Y-chromosome sequence was recently identified over 2 scaffolds in the reference genome. Here, these scaffolds were scanned for repeated motifs, revealing 194 likely microsatellites. Twenty-three of these loci were selected for primer development and, after testing, produced a panel of 11 novel markers that were analyzed alongside 2 markers previously developed for the red fox from dog Y-chromosome sequence. The markers were genotyped in 76 male red foxes from 4 populations: 7 foxes from Newfoundland (eastern Canada), 12 from Maryland (eastern United States), and 9 from the island of Great Britain, as well as 48 foxes of known North American origin maintained on an experimental farm in Novosibirsk, Russia. The full marker panel revealed 22 haplotypes among these red foxes, whereas the 2 previously known markers alone would have identified only 10 haplotypes. The haplotypes from the 4 populations clustered primarily by continent, but unidirectional gene flow from Great Britain and farm populations may influence haplotype diversity in the Maryland population. The development of new markers has increased the resolution at which red fox Y-chromosome diversity can be analyzed and provides insight into the contribution of males to red fox population diversity and patterns of phylogeography
Data from: Y-chromosome markers for the red fox
The de novo assembly of the red fox (Vulpes vulpes) genome has facilitated the development of genomic tools for the species. Efforts to identify the population history of red foxes in North America have previously been limited by a lack of information about the red fox Y-chromosome sequence. However, a megabase of red fox Y-chromosome sequence was recently identified over 2 scaffolds in the reference genome. Here, these scaffolds were scanned for repeated motifs, revealing 194 likely microsatellites. Twenty-three of these loci were selected for primer development and, after testing, produced a panel of 11 novel markers that were analyzed alongside 2 markers previously developed for the red fox from dog Y-chromosome sequence. The markers were genotyped in 76 male red foxes from 4 populations: 7 foxes from Newfoundland (eastern Canada), 12 from Maryland (eastern United States), and 9 from the island of Great Britain, as well as 48 foxes of known North American origin maintained on an experimental farm in Novosibirsk, Russia. The full marker panel revealed 22 haplotypes among these red foxes, whereas the 2 previously known markers alone would have identified only 10 haplotypes. The haplotypes from the 4 populations clustered primarily by continent, but unidirectional gene flow from Great Britain and farm populations may influence haplotype diversity in the Maryland population. The development of new markers has increased the resolution at which red fox Y-chromosome diversity can be analyzed and provides insight into the contribution of males to red fox population diversity and patterns of phylogeography