A method description and feasibility study

A detailed transabdominal and transvaginal ultrasound examination, performed by an expert examiner, could render a similar diagnostic performance to computed tomography for assessing pelvic/abdominal tumor spread disease in women with epithelial ovarian cancer (EOC). This study aimed to describe and assess the feasibility of lung and intercostal upper abdomen ultrasonography as pretreatment imaging of EOC metastases of supradiaphragmatic and subdiaphragmatic areas. A preoperative ultrasound examination of consecutive patients suspected of having EOC was prospectively performed using transvaginal, transabdominal, and intercostal lung and upper abdomen ultrasonography. A surgical-pathological examination was the reference standard to ultrasonography. Among 77 patients with histologically proven EOC, supradiaphragmatic disease was detected in 13 cases: pleural effusions on the right (n = 12) and left (n = 8) sides, nodular lesions on diaphragmatic pleura (n = 9), focal lesion in lung parenchyma (n = 1), and enlarged cardiophrenic lymph nodes (n = 1). Performance (described with area under the curve) of combined transabdominal and intercostal upper abdomen ultrasonography for subdiaphragmatic areas (n = 77) included the right and left diaphragm peritoneum (0.754 and 0.575 respectively), spleen hilum (0.924), hepatic hilum (0.701), and liver and spleen parenchyma (0.993 and 1.0 respectively). It was not possible to evaluate the performance of lung ultrasonography for supradiaphragmatic disease because only some patients had this region surgically explored. Preoperative lung and intercostal upper abdomen ultrasonography performed in patients with EOC can add valuable information for supradiaphragmatic and subdiaphragmatic regions. A reliable reference standard to test method performance is an area of future research. A multidisciplinary approach to ovarian cancer utilizing lung ultrasonography may assist in clinical decision-making.publishersversionpublishe

Lung and Intercostal Upper Abdomen Ultrasonography for Staging Patients with Ovarian Cancer: A Method Description and Feasibility Study

A detailed transabdominal and transvaginal ultrasound examination, performed by an expert examiner, could render a similar diagnostic performance to computed tomography for assessing pelvic/abdominal tumor spread disease in women with epithelial ovarian cancer (EOC). This study aimed to describe and assess the feasibility of lung and intercostal upper abdomen ultrasonography as pretreatment imaging of EOC metastases of supradiaphragmatic and subdiaphragmatic areas. A preoperative ultrasound examination of consecutive patients suspected of having EOC was prospectively performed using transvaginal, transabdominal, and intercostal lung and upper abdomen ultrasonography. A surgical-pathological examination was the reference standard to ultrasonography. Among 77 patients with histologically proven EOC, supradiaphragmatic disease was detected in 13 cases: pleural effusions on the right (n = 12) and left (n = 8) sides, nodular lesions on diaphragmatic pleura (n = 9), focal lesion in lung parenchyma (n = 1), and enlarged cardiophrenic lymph nodes (n = 1). Performance (described with area under the curve) of combined transabdominal and intercostal upper abdomen ultrasonography for subdiaphragmatic areas (n = 77) included the right and left diaphragm peritoneum (0.754 and 0.575 respectively), spleen hilum (0.924), hepatic hilum (0.701), and liver and spleen parenchyma (0.993 and 1.0 respectively). It was not possible to evaluate the performance of lung ultrasonography for supradiaphragmatic disease because only some patients had this region surgically explored. Preoperative lung and intercostal upper abdomen ultrasonography performed in patients with EOC can add valuable information for supradiaphragmatic and subdiaphragmatic regions. A reliable reference standard to test method performance is an area of future research. A multidisciplinary approach to ovarian cancer utilizing lung ultrasonography may assist in clinical decision-making.info:eu-repo/semantics/publishedVersio

Вплив концентрації бактерій на протимікробну активність декаметоксину = Influence of concentration on bacteria the activity the antimicrobial activity decamethoxinum

Stukan Oksana. Вплив концентрації бактерій на протимікробну активність декаметоксину = Influence of concentration on bacteria the activity the antimicrobial activity decamethoxinum. Journal of Education, Health and Sport. 2015;5(6):291-296. ISSN 2391-8306. DOI 10.5281/zenodo.18614http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%286%29%3A291-296https://pbn.nauka.gov.pl/works/566838http://dx.doi.org/10.5281/zenodo.18614Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X. Archives 2011 – 2014 http://journal.rsw.edu.pl/index.php/JHS/issue/archive Deklaracja.Specyfika i zawartość merytoryczna czasopisma nie ulega zmianie.Zgodnie z informacją MNiSW z dnia 2 czerwca 2014 r., że w roku 2014 nie będzie przeprowadzana ocena czasopism naukowych; czasopismo o zmienionym tytule otrzymuje tyle samo punktów co na wykazie czasopism naukowych z dnia 31 grudnia 2014 r.The journal has had 5 points in Ministry of Science and Higher Education of Poland parametric evaluation. Part B item 1089. (31.12.2014).© The Author (s) 2015;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland and Radom University in Radom, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 21.04.2015. Revised 28.05.2015. Accepted: 14.06.2015. УДК; 615.27 ВПЛИВ КОНЦЕНТРАЦІЇ БАКТЕРІЙ НА ПРОТИМІКРОБНУ АКТИВНІСТЬ ДЕКАМЕТОКСИНУInfluence of concentration on bacteria the activity the antimicrobial activity decamethoxinum О. К. СтуканOksana Stukan ВНМУ ім.М.І.Пирогова, кафедра мікробіології, вірусології та імунології, ВінницяVinnitsky National Medical University named M.I.Pirogova, department of Microbiology Abstract The use domestic antiseptic preparations in medicine allowed considerably improve the efficiency prophylaxis and treatment of of many diseases infectious genesis. It is known that they are active in in the presence of the products tissue decay; operate in acidic and alkaline environments do not cause local irritating. Preparations fixed form based decamethoxinum have inhibitory and the bactericidal action on bacteria, viruses, fungi and detoxic effect on staphylococcal, diphtheritic exotoxin, possess good regenerative effect against allergies. The study was conducted at clinical strains of Staphylococcus aureus by standard serial dilutions. The results showed high activity and effectiveness of the drug decamethoxinum. The use of domestic of antiseptics preparations in medicine allowed considerably improved the effectiveness prophylaxis and treatment of many diseases of infectious genesis. It is known that they are active in the presence of the products tissue decay; operate in acidic and an alkaline environments do not cause local irritating. Investigation of antimicrobial activity decamethoxinum (DCM), carried out in clinical strains of Staphylococcus aureus. From different medical patients, prophylactic establishments, we have removed and been identified 187 clinical strains of microorganisms. Results of the study of antimicrobial activity decamethoxinum on clinical strains of Staphylococcus aureus have shown the following. The minimum bacteriostatic the concentration of DCM was equal to 0,75 ± 0,06 mg / ml, the minimum bactericidal concentration DCM has increased to 1,5 ± 0,06 mg / ml. The dependence of the biological activity of antimicrobial compounds on the number of microorganisms due to a decrease the number of available of molecules existing centers the active compound of by microorganisms in the conditions of the saturated substrate. In the process of studying of sowing dose test microorganisms to antimicrobial activity of antiseptics preparations the following results. To explore dependence of actions on the magnitude of the microbial load in a clinical setting, we studied the effect DCM clinical strains are Staphylococcus aureus. MIC DCM for clinical strains of microbial load 10³ CFU / ml was equal 4,56 ± 0,93mkh / ml, while the load 106 CFU / mL DCM MIC was equal to 4,69 ± 1,6 mg / ml. MCC DCM microbial load of 109 cfu / ml was 9,57 ± 3,96 mg / ml.Conclusion. Investigational antiseptic dasg decamethoxin has a wide range of antimicrobial activity on causative agents of nosocomial infections, retains a high antimicrobial activity, quickly detoxifies microorganisms. Our results demonstrated strong the activity of DCM clinical strains are Staphylococcus.Key words: staphylococcus, clinical strains, CFU, decamethoxinum, antimicrobial activity, concentration  РезюмеВлияние концентрации бактерий на противомикробную активность декаметоксина. Использование отечественных антисептических препартов в медицине позволило значительно улучшить эфективность профилактики и лечения госпитальных заболеваний инфекционного генеза. Известно, что они проявляют активность в присутствии продуктов тканевого разпада; действуют в кислой и щелочной среде, не вызывают местного раздражающего эффекта. Лекарственные формы на основе декаметоксина владеют бактериостатическим и микробоцидным действием на бактерии, вирусы, грибы, а также имеют иннактивирующее действие на стафилококовый, дифтерийный экзотоксины, имеют отличное регенераторное и противоалергическое действие. Исследование проведено на клинических штаммах стафилококка методом стандартных серийных разведений. Результаты исследований показали высокую активность и эффективность препарата декаметоксина.Ключевые слова: стафилококк, клинические штаммы, КОЕ декаметоксин, антимикробная активность, концентрация. РезюмеЗастосування вітчизняного антисептичного препаратів в медицині дозволило значно покращити ефективність профилактики та лікування госпітальних хвороб інфекційного генезу. Відомо, що вони проявляють активність в присутності продуктів тканинного розпаду; діють в кислому та лужному середовищах, не викликають місцеву подразнювальну дію. Лікарські фіксовані форми на основі декаметоксину володіють бактеріостатичною та мікробоцидною дією щодо бактерій, вірусів, грибів, а також детоксикуючою дією щодо стафілококового, дифтерійного екзотоксинів, мають гарну регенеративну та протиалергійну дію. Дослідження проведено на клінічних штамах стафілокока методом стандартних серійних розведень. Результати досліджень показали високу активність та ефективність препарату декаметоксину.Ключові слова: стафілокок, клінічні штами, КУО, декаметоксин, антимікробна активність, концентрація

Ultrasound and clinical preoperative characteristics for discrimination between ovarian metastatic colorectal cancer and primary ovarian cancer: A case-control study

The aim of this study was to describe the clinical and sonographic features of ovarian metastases originating from colorectal cancer (mCRC), and to discriminate mCRC from primary ovarian cancer (OC). We conducted a multi-institutional, retrospective study of consecutive patients with ovarian mCRC who had undergone ultrasound examination using the International Ovarian Tumor Analysis (IOTA) terminology, with the addition of evaluating signs of necrosis and abdominal staging. A control group included patients with primary OC. Clinical and ultrasound data, subjective assessment (SA), and an assessment of different neoplasias in the adnexa (ADNEX) model were evaluated. Fisher's exact and Student's t-tests, the area under the receiver-operating characteristic curve (AUC), and classification and regression trees (CART) were used to conduct statistical analyses. In total, 162 patients (81 with OC and 81 with ovarian mCRC) were included. None of the patients with OC had undergone chemotherapy for CRC in the past, compared with 40% of patients with ovarian mCRC (p < 0.001). The ovarian mCRC tumors were significantly larger, a necrosis sign was more frequently present, and tumors had an irregular wall or were fixed less frequently; ascites, omental cake, and carcinomatosis were less common in mCRC than in primary OC. In a subgroup of patients with ovarian mCRC who had not undergone treatment for CRC in anamnesis, tumors were larger, and had fewer papillations and more locules compared with primary OC. The highest AUC for the discrimination of ovarian mCRC from primary OC was for CART (0.768), followed by SA (0.735) and ADNEX calculated with CA-125 (0.680). Ovarian mCRC and primary OC can be distinguished based on patient anamnesis, ultrasound pattern recognition, a proposed decision tree model, and an ADNEX model with CA-125 levels

Giant ovarian tumor – case report and proposal for clinical management

Abstract A case report of a woman with 90kg ovarian tumor is presented. Paracentesis and intravenous alimentation was performed before operation. During the surgery, the ovarian tumor was excised and abdominal cavity packing, followed by slow pressure decreasing, was done. Intravenous together with oral alimentation was continued after the operation. The patient was discharged on the 10th postoperative day, without any complications, in a good overall condition

Equation of State for Macromolecules of Variable Flexibility in Good Solvents: A Comparison of Techniques for Monte Carlo Simulations of Lattice Models

The osmotic equation of state for the athermal bond fluctuation model on the simple cubic lattice is obtained from extensive Monte Carlo simulations. For short macromolecules (chain length N=20) we study the influence of various choices for the chain stiffness on the equation of state. Three techniques are applied and compared in order to critically assess their efficiency and accuracy: the repulsive wall method, the thermodynamic integration method (which rests on the feasibility of simulations in the grand canonical ensemble), and the recently advocated sedimentation equilibrium method, which records the density profile in an external (e.g. gravitation-like) field and infers, via a local density approximation, the equation of state from the hydrostatic equilibrium condition. We confirm the conclusion that the latter technique is far more efficient than the repulsive wall method, but we find that the thermodynamic integration method is similarly efficient as the sedimentation equilibrium method. For very stiff chains the onset of nematic order enforces the formation of isotropic-nematic interface in the sedimentation equilibrium method leading to strong rounding effects and deviations from the true equation of state in the transition regime.Comment: 32 pages, 18 figures, submitted to Phys.Rev.E; one paragraph added to conclusions sectio

Influence of solvent quality on polymer solutions: a Monte Carlo study of bulk and interfacial properties

The effect of solvent quality on dilute and semi-dilute regimes of polymers in solution is studied by means of Monte Carlo simulations. The equation of state, adsorptions near a hard wall, wall-polymer surface tension and effective depletion potentials are all calculated as a function of concentration and solvent quality. We find important differences between polymers in good and theta solvents. In the dilute regime, the physical properties for polymers in a theta solvent closely resemble those of ideal polymers. In the semi-dilute regime, however, significant differences are found.Comment: 10 pages, 13 figure

Клиническая значимость Р16-позитивного статуса и высокой пролиферативной активности у пациентов с орофарингеальной плоскоклеточной карциномой

Introduction. In accordance with uICC and AJCC 8th edition TNM classifications, there is a strong evidence for division of oropharyngeal squamous cell carcinoma (OPSCC) into 2 molecular subtypes by HPV-status with distinct prognosis depending on biological differences. Such a division leads to differences in staging OPSCC and in future it will lead to implementation of preventive measures and new therapeutic strategies against HPV-positive cancer.Aim of the study: to assess the clinical and prognostic significance of the combination of P16, a surrogate marker for HPV-positivity, and high proliferative activity in patients with oropharyngeal carcinoma.Material and Methods. Immunohistochemical (ICH) analysis with monoclonal antibodies specific for P16 and Ki67 proteins was used to detect expression patterns in the formalin-fixed, paraffin-embedded tumor samples obtained from 104 patients with squamous cell carcinoma of the tongue and oropharynx, treated at Oncological Dispencery № 1 in Krasnodar from 2011 to 2016. HPV-positive status was determined if more than 70 % of tumor cells had moderate or strong nuclear and cytoplasmic P16-staining. High index of proliferative activity (PA) was detected if more than 50 % tumor cells expressed Ki67 nuclear antigen.Results. P16-positivie status was associated with tonsillar cancer (р=0.002), female gender (р=0.015), age <60 years (р<0.001), non - keratinizing morphology (р=0.022), and high index of PA (p=0.01).The combination of P16>70 % with high PA demonstrated correlation with tonsillar cancer (р<0.001), female gander (р=0.015), age under 60 years (р<0.001) and non - keratinizing morphology (р=0.012). HPV-positive patients and patients with a combination of P16>70 % and high index of PA at N1-2 had an overall survival benefit (p=0.021).Conclusion. The correlation between IHC-complex for P16>70 %/Ki67>50 % and clinicopathologicl parameters and overall survival confirms the biological features of HPV-associated cancer. The evaluation of this IHC-complex can increase the diagnostic accuracy of IHC-analysis of HPV-status and predict the prognosis of patients with OPSCC.Введение. По классификации TNM UICC/AJCC 8-го пересмотра предлагается разделить орофарингеальную плоскоклеточную карциному на 2 молекулярных подтипа по ВПЧ-статусу с различными клиническими и прогностическими характеристиками ввиду биологических особенностей. Так, имеются отличия в подходах к стадированию орофарингеального рака, и в будущем это приведет к реализации профилактических мероприятий и внедрению новых терапевтических стратегий в отношении ВПЧ-позитивного рака.Цель исследования - оценить клинико-прогностическую значимость комбинации суррогатного маркера ВПЧ-позитивного статуса P16 с высокой пролиферативной активностью при орофарингеальном раке.Материал и методы. Иммуногистохимический анализ выполнен с моноклональными антителами к P16 и Ki67 на срезах с парафиновых гистоблоков опухолевых образцов 104 больных плоскоклеточным раком языка и ротоглотки, проходивших лечение в ГБУЗ КОД № 1 г Краснодара в 2011-16 гг Р16-положительный статус определяли при умеренном или сильном ядерном и цитоплазматическом окрашивании более 70 % опухолевых клеток. Высокий индекс пролиферативной активности устанавливался, если более 50 % опухолевых клеток имели ядерное окрашивание антигена Ki67.Результаты. Р16-позитивный статус был связан с раком нёбных миндалин (р=0,002), характерен для женщин (р=0,015), ассоциирован с возрастом моложе 60 лет (р<0,001), морфологией без ороговения (р=0,022), высокой пролиферацией (р=0,01). Сочетание Р16-позитивного статуса с высокой пролиферацией продемонстрировало корреляцию с раком нёбных миндалин (р<0,001), женским полом (р=0,015), возрастом моложе 60 лет (р<0,001) и морфологией без ороговения (р=0,012). ВПЧ-позитивные больные и пациенты с комбинацией Р16-позитивного статуса и высокого индекса пролиферативной активности с метастазами лимфатических узлов (N1-2) имели более высокие показатели 3-летней общей выживаемости (p=0,021).Вывод. Корреляция ИГХкомплекса Р16-позитивности и высокой пролиферативной активности с клинико-морфологическими особенностями и общей выживаемостью подтверждает особенности биологии ВПЧ-ассоциированного рака. Исследование комбинации индекса пролиферативной активности в дополнение к суррогатному маркеру ВПЧ-статуса приведет к повышению диагностической точности ИГХ-анализа ВПЧ-статуса и позволит определять прогноз пациентов с плоскоклеточным раком головы и шеи

Zapalenie wyrostka robaczkowego i choroby pęcherzyka żółciowego jako stany ostre wymagające pilnej interwencji chirurgicznej u kobiet w ciąży

Appendicitis (APP) and gall bladder diseases (GBD) are the most frequent non-obstetric indications for urgent surgery among pregnant women. The aim was to present the diagnosis, treatment and potential complications of APP and symptomatic GBD. We searched the literature for APP and GBD during pregnancy and presented the results in the form of a review article. APP symptoms among pregnant women are comparable to these in the general population. Typical clinical symptoms are present in 50-75% of cases. Laboratory tests are useful for a differential diagnosis. The imaging of choice is an ultrasonography scan, but magnetic resonance is of the highest accuracy. The final diagnosis is difficult. When the surgery is delayed, the risk of appendix perforation increases and thus complications are more frequent. GBD symptoms and signs are comparable to those in the general population. The best imaging is an ultrasonography scan, and laboratory tests are important in a jaundice differential diagnosis. In cases with symptomatic GBD, a delay in surgery is associated with an increased risk of complications (pancreatitis, abortion, intrauterine death). The treatment method of choice for APP and symptomatic GBD is surgery, both laparotomy and laparoscopy (preferred), which are considered relatively safe, though laparoscopy compared to laparotomy for APP can be associated with a higher risk of abortion. Untreated or delayed APP and symptomatic GBD treatment during pregnancy increases the risk of complications, both for the woman and the fetus. Diagnosis is difficult and should be based on a multidisciplinary approach to the patient. Surgery by laparotomy or laparoscopy is relatively safe.Zapalenie wyrostka robaczkowego (APP) i choroby pęcherzyka żółciowego (GBD) są najczęstszymi, nie położniczymi wskazaniami do pilnej operacji chirurgicznej u ciężarnych. Celem pracy było przedstawienie diagnostyki, leczenia i potencjalnych powikłań APP i objawowych GBD. Dokonano przeglądu piśmiennictwa na temat APP i GBD u ciężarnych i opisano w formie pracy poglądowej. Wywiad u chorych ciężarnych z APP jest podobny jak w populacji ogólnej, zaś typowe objawy przedmiotowe są stwierdzane u 50-75%. Badania laboratoryjne pozwalają wykluczyć inne jednostki chorobowe. Metodą diagnostyki obrazowej jest ultrasonografia, jednak rezonans magnetyczny ma największą dokładność. Rozpoznanie jest trudne i opiera się na łącznej analizie wyników wszystkich badań. Odraczanie operacji powoduje wzrost ryzyka perforacji wyrostka robaczkowego, w konsekwencji częstsze niepowodzenia położnicze, oraz powikłania dla chorej. Wywiad i badanie kliniczne u ciężarnych z objawowymi GBD są podobne jak w populacji ogólnej. Metodą obrazowania jest ultrasonografia. Ważne są badania laboratoryjne, szczególnie w różnicowaniu żółtaczek. Odraczanie leczenia operacyjnego objawowych GBD wiąże się ze zwiększonym ryzykiem powikłań u ciężarnej (zapalenie trzustki) oraz niepowodzeń położniczych (poronienia, zgon wewnątrzmaciczny płodu). Postępowaniem z wyboru w przypadkach APP i objawowych GBD jest leczenie chirurgiczne przez laparotomię lub laparoskopię, z których ta druga jest metodą zalecaną. Obie metody są relatywnie bezpieczne, choć laparoskopia w porównaniu z laparotomią w przypadku APP może zwiększać ryzyko poronień