Interplay

"Interplay" is a kinetic dance which emphasizes different energy levels, line design, spacial relationships, and motion. The central idea of the dance is based upon the interplay of the two main kinetic themes--abrupt angular motions and flowing circular motions. Different aspects of the two basic themes are discovered as they are manipulated, contrasted, combined, and energized at different levels and tempos. The effectiveness of the spacial relationships requires the usage of five dancers. The music, Anton Webern's "Opus 7: Four Pieces For Violin and Piano" and "Opus 6: Six Pieces For Orchestra," acts as a background for the movement themes. The kinetic content of the dance dictated an ABCD structure. Each section shows different facets and interactions of the two general themes. The first section presents the angular motif and the circular motif in sharp contrast to one another. Further contrast between the two themes is explored in the second section as more locomotive movement is used

A Study Of Methods Of Teaching Reading Comprehension In Grades Five, Six, And Seven

The purpose of this study was to learn what methods fifth, sixth, and seventh grade teachers have found helpful in teaching each of the various phases of comprehension separately and concurrently in a normal classroom situation

Disruption of host selenobiology by SARS-CoV-2 and Ebola virus via RNA:RNA antisense interactions

Selenium is an essential, but often overlooked, element in maintaining the health of our biological systems. Occupying the redox center of different selenoproteins, such as thioredoxin reductase (TXNRD) or glutathione peroxidase as selenocysteine (SeC), allows them to perform various unique biological functions, e.g., redox regulation and antioxidant defense. The synthesis of selenoproteins involves the reprogramming of a UGA stop codon to allow the recruitment of a SeC t-RNA. This occurs during translation, using a unique RNA structure called a selenocysteine insertion sequence (SECIS) element. The SECIS element can be located within the mRNA being translated but can also be hijacked from a second RNA molecule through antisense tethering. This tethering can also facilitate another translation event in the form of ribosomal frameshifting that allows genes to encode for multiple proteins by overlapping reading frames. Our research group has previously identified such frameshift sites in several pathogenic RNA viruses, including Ebola virus (EBOV) and HIV-1. This antisense tethering could enhance the frameshifting event to allow the recoding of those UGA codons as SeC. Evidence will be presented for these events being programmed in the genomes of EBOV and SARS-CoV-2. The EBOV nucleoprotein gene is an example of the reprogramming of a UGA stop codon as a potential SeC codon. Using green fluorescent protein as a downstream reporter gene, we show selenium-dependent read-through of the 3’-UGA stop codon, which is highly conserved only in the most virulent strains of EBOV. In the EBOV polymerase (L) gene, a programmed ribosomal frameshift signal leads to an overlapping gene with 2 tandem UGA codons immediately followed by an RNA region that is the inverse complement (antisense) to a region of the selenoprotein iodothyronine deiodinase II (DIO2) mRNA, which could both trigger the ribosomal frameshift and provide access to the SECIS element contained in the DIO2 mRNA. We have designed an innovative assay for -1 frameshifting at such sites in which upstream and downstream reporter genes are used to assess the initiation and termination of protein synthesis. By inserting a wildtype or mutant viral insert, we show a highly significant level of -1 ribosomal frameshifting at the EBOV L gene site mentioned above. In SARS-CoV-2, we have demonstrated an antisense interaction between a region of the viral RNA and the mRNA for TXNRD3, another selenoprotein that is highly expressed in the human testes. This predicted antisense interaction was confirmed experimentally by knockdown of TXNRD3 mRNA in SARS-CoV-2 infected Vero cells. The significance of these findings regarding links between selenium status and the severity of Covid-19, and EBOV infection, will be discussed

Effect of rye bread breakfasts on subjective hunger and satiety: a randomized controlled trial

<p>Abstract</p> <p>Background</p> <p>Several studies report that dietary fibre from different sources promotes the feeling of satiety and suppresses hunger. However, results for cereal fibre from rye are essentially lacking. The aim of the present study was to investigate subjective appetite during 8 h after intake of iso-caloric rye bread breakfasts varying in rye dietary fibre composition and content.</p> <p>Methods</p> <p>The study was divided into two parts. The first part (n = 16) compared the satiating effect of iso-caloric bread breakfasts including different milling fractions of rye (bran, intermediate fraction (B4) and sifted flour). The second part (n = 16) investigated the dose-response effect of rye bran and intermediate rye fraction, each providing 5 or 8 g of dietary fibre per iso-caloric bread breakfast. Both study parts used a wheat bread breakfast as reference and a randomised, within-subject comparison design. Appetite (hunger, satiety and desire to eat) was rated regularly from just before breakfast at 08:00 until 16:00. Amount, type and timing of food and drink intake were standardised during the study period.</p> <p>Results</p> <p>The Milling fractions study showed that each of the rye breakfasts resulted in a suppressed appetite during the time period before lunch (08:3012:00) compared with the wheat reference bread breakfast. At a comparison between the rye bread breakfasts the one with rye bran induced the strongest effect on satiety. In the afternoon the effect from all three rye bread breakfasts could still be seen as a decreased hunger and desire to eat compared to the wheat reference bread breakfast.</p> <p>In the Dose-response study both levels of rye bran and the lower level of intermediate rye fraction resulted in an increased satiety before lunch compared with the wheat reference bread breakfast. Neither the variation in composition between the milling fractions nor the different doses resulted in significant differences in any of the appetite ratings when compared with one another.</p> <p>Conclusion</p> <p>The results show that rye bread can be used to decrease hunger feelings both before and after lunch when included in a breakfast meal. Rye bran induces a stronger effect on satiety than the other two rye fractions used when served in iso-caloric portions.</p> <p>Trial Registration</p> <p>Trial registration number NCT00876785</p

A barley cysteine-protease inhibitor reduces teh performance of two aphid species in artificial diets and transgenic arabidopsis plants

Cystatins from plants have been implicated in plant defense towards insects, based on their role as inhibitors of heterologous cysteine-proteinases. We have previously characterized thirteen genes encoding cystatins (HvCPI-1 to HvCPI-13) from barley (Hordeum vulgare), but only HvCPI-1 C68 → G, a variant generated by direct-mutagenesis, has been tested against insects. The aim of this study was to analyze the effects of the whole gene family members of barley cystatins against two aphids, Myzus persicae and Acyrthosiphon pisum. All the cystatins, except HvCPI-7, HvCPI-10 and HvCPI-12, inhibited in vitro the activity of cathepsin L- and/or B-like proteinases, with HvCPI-6 being the most effective inhibitor for both aphid species. When administered in artificial diets, HvCPI-6 was toxic to A. pisum nymphs (LC50 = 150 μg/ml), whereas no significant mortality was observed on M. persicae nymphs up to 1000 μg/ml. The effects of HvCPI-6 ingestion on A. pisum were correlated with a decrease of cathepsin B- and L-like proteinase activities. In the case of M. persicae, there was an increase of these proteolytic activities, but also of the aminopeptidase-like activity, suggesting that this species is regulating both target and insensitive enzymes to overcome the effects of the cystatin. To further analyze the potential of barley cystatins as insecticidal proteins against aphids, Arabidopsis plants expressing HvCPI-6 were tested against M. persicae. For A. pisum, which does not feed on Arabidopsis, a combined diet-Vicia faba plant bioassay was performed. A significant delay in the development time to reach the adult stage was observed in both species. The present study demonstrates the potential of barley cystatins to interfere with the performance of two aphid specie

The Na+/H+ Exchanger Controls Deoxycholic Acid-Induced Apoptosis by a H+-Activated, Na+-Dependent Ionic Shift in Esophageal Cells

Apoptosis resistance is a hallmark of cancer cells. Typically, bile acids induce apoptosis. However during gastrointestinal (GI) tumorigenesis the cancer cells develop resistance to bile acid-induced cell death. To understand how bile acids induce apoptosis resistance we first need to identify the molecular pathways that initiate apoptosis in response to bile acid exposure. In this study we examined the mechanism of deoxycholic acid (DCA)-induced apoptosis, specifically the role of Na+/H+ exchanger (NHE) and Na+ influx in esophageal cells. In vitro studies revealed that the exposure of esophageal cells (JH-EsoAd1, CP-A) to DCA (0.2 mM -0.5 mM) caused lysosomal membrane perturbation and transient cytoplasmic acidification. Fluorescence microscopy in conjunction with atomic absorption spectrophotometry demonstrated that this effect on lysosomes correlated with influx of Na+, subsequent loss of intracellular K+, an increase of Ca2+ and apoptosis. However, ethylisopropyl-amiloride (EIPA), a selective inhibitor of NHE, prevented Na+, K+ and Ca2+ changes and caspase 3/7 activation induced by DCA. Ouabain and amphotericin B, two drugs that increase intracellular Na+ levels, induced similar changes as DCA (ion imbalance, caspase3/7 activation). On the contrary, DCA-induced cell death was inhibited by medium with low a Na+ concentrations. In the same experiments, we exposed rat ileum ex-vivo to DCA with or without EIPA. Severe tissue damage and caspase-3 activation was observed after DCA treatment, but EIPA almost fully prevented this response. In summary, NHE-mediated Na+ influx is a critical step leading to DCA-induced apoptosis. Cells tolerate acidification but evade DCA-induced apoptosis if NHE is inhibited. Our data suggests that suppression of NHE by endogenous or exogenous inhibitors may lead to apoptosis resistance during GI tumorigenesis

Quantitative High-Throughput Screening Identifies 8-Hydroxyquinolines as Cell-Active Histone Demethylase Inhibitors

Small molecule modulators of epigenetic processes are currently sought as basic probes for biochemical mechanisms, and as starting points for development of therapeutic agents. N(epsilon)-Methylation of lysine residues on histone tails is one of a number of post-translational modifications that together enable transcriptional regulation. Histone lysine demethylases antagonize the action of histone methyltransferases in a site- and methylation state-specific manner. N(epsilon)-Methyllysine demethylases that use 2-oxoglutarate as co-factor are associated with diverse human diseases, including cancer, inflammation and X-linked mental retardation; they are proposed as targets for the therapeutic modulation of transcription. There are few reports on the identification of templates that are amenable to development as potent inhibitors in vivo and large diverse collections have yet to be exploited for the discovery of demethylase inhibitors

Optical imaging in vivo with a focus on paediatric disease: technical progress, current preclinical and clinical applications and future perspectives

To obtain information on the occurrence and location of molecular events as well as to track target-specific probes such as antibodies or peptides, drugs or even cells non-invasively over time, optical imaging (OI) technologies are increasingly applied. Although OI strongly contributes to the advances made in preclinical research, it is so far, with the exception of optical coherence tomography (OCT), only very sparingly applied in clinical settings. Nevertheless, as OI technologies evolve and improve continuously and represent relatively inexpensive and harmful methods, their implementation as clinical tools for the assessment of children disease is increasing. This review focuses on the current preclinical and clinical applications as well as on the future potential of OI in the clinical routine. Herein, we summarize the development of different fluorescence and bioluminescence imaging techniques for microscopic and macroscopic visualization of microstructures and biological processes. In addition, we discuss advantages and limitations of optical probes with distinct mechanisms of target-detection as well as of different bioluminescent reporter systems. Particular attention has been given to the use of near-infrared (NIR) fluorescent probes enabling observation of molecular events in deeper tissue

Trust-building strategies in corporate discourse:An experimental study

This paper presents a scenario-based experiment designed to test the effects of trust-building strategies, realised in stance-taking acts, which a previous corpus-based study found to be salient features of stakeholder-facing corporate communication. The experiment relies on a between-subjects design in which a target group of subjects are exposed to trust-building strategies while another control group are not. We apply this paradigm to corporate discourse in the form of an About Us webpage produced by a fictitious multinational pharmaceutical company that has been accused by a whistleblower of corporate misconduct. The results of the study show that these strategies are indeed effective in fostering trust in the company and have an indirect positive effect on the perceived credibility of the company’s denial in response to the allegations made by the whistleblower. The strategies are therefore able to mitigate the potential damage caused by public accusations of wrongdoing and help companies insure against future threats to their legitimacy and freedom to operate, as when their behaviour violates, or is said to violate, societal norms and values. Theoretically, the results provide insights into the psychological mechanisms of trust-building and reader response. Methodologically, the study contributes to the growing body of work using experimental methods in CDA by further demonstrating that experimentation can usefully complement more traditional discourse-analytical methods as a form of triangulation