The Utilization of Polymerase Chain Reaction, DNA Barcoding and Bioinformatics in Identifying Plant Species

Bioinformatics and DNA barcoding is a process used to identify plants, animals, and fungi. DNA barcoding in plants utilizes a key variable region in the genome, the RuBisCo large subunit (RbcL) on Chloroplast DNA. Once the DNA is extracted, Polymerase Chain Reaction (PCR) amplifies that region and that sample is sent off for sequencing. Bioinformatics and DNA barcoding helps taxonomists determine the sequence of the RbcL gene as well as obtain a unique barcode that can be used to identify plants. Several plant species from our local campus were sequenced and identified using the previously described methods

αV-Integrins Are Required for Mechanotransduction in MDCK Epithelial Cells

The properties of epithelial cells within tissues are regulated by their immediate microenvironment, which consists of neighboring cells and the extracellular matrix (ECM). Integrin heterodimers orchestrate dynamic assembly and disassembly of cell-ECM connections and thereby convey biochemical and mechanical information from the ECM into cells. However, the specific contributions and functional hierarchy between different integrin heterodimers in the regulation of focal adhesion dynamics in epithelial cells are incompletely understood. Here, we have studied the functions of RGD-binding αV-integrins in a Madin Darby Canine Kidney (MDCK) cell model and found that αV-integrins regulate the maturation of focal adhesions (FAs) and cell spreading. αV-integrin-deficient MDCK cells bound collagen I (Col I) substrate via α2β1-integrins but failed to efficiently recruit FA components such as talin, focal adhesion kinase (FAK), vinculin and integrin-linked kinase (ILK). The apparent inability to mature α2β1-integrin-mediated FAs and link them to cellular actin cytoskeleton led to disrupted mechanotransduction in αV-integrin deficient cells seeded onto Col I substrate

Human oestriasis acquired in Florence and review on human myiasis in Italy

Myiases-causing flies are widely observed in tropical countries, whereas in Italy, a temperate country, their epidemiology and clinical presentation are poorly known. We report three cases of human conjunctival myiasis recently observed at our hospital, and the results of a review of the literature on human myiasis in Italy. In August 2012, a case of Oestrus ovis conjunctival myiasis acquired in the city centre of Florence, Italy was diagnosed at our hospital. In the early fall of 2013, two additional cases, acquired in the neighbouring areas, occurred. The review of literature showed that, up to the middle of 1990s, myiasis in Italy was mainly an occupational disease of shepherds, caused by O. ovis. Recently, cases of travel acquired furuncular myiasis emerged, together with “opportunistic” autochthonous cases of wound myiasis in patients with underlying health conditions. Considering the causative agents of human of myiasis in Italy, among the 703 autochthonous cases reported, 98.1 % were caused by O. ovis, while among the 42 imported cases described, 59.5 % were due to Cordylobia spp. and 40.5 % to Dermatobia hominis. Our findings suggest that O. ovis conjunctival myiasis may still be observed in urban setting in Italy. Health care providers should know and implement the basic rules of entomoprophylaxis for myiasis in the facilities where they are working and use these indications to educate patients and care givers in both pretravel care and geriatric outpatient settings. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00436-014-3906-9) contains supplementary material, which is available to authorized users

Systemevaluierung "KMU-innovativ"

Der vorliegende Bericht stellt die Ergebnisse einer Systemevaluierung der Förderinitiative KMU-innovativ des BMBF dar. Die Evaluierung startete Ende 2008 und damit ein Jahr nach dem Beginn der Förderinitiative im Herbst 2007. Sie hatte zum Ziel, die Implementation, Zielerreichung und Wirkung der Förderinitiative sowie ihre Position in der Förderlandschaft zu bewerten. Die hier vorgelegten Ergebnisse bilden die Erfahrungen der ersten dreieinhalb Jahre der Umsetzung von KMU-innovativ ab

EU-Rotate_N – a decision support system – to predict environmental and economic consequences of the management of nitrogen fertiliser in crop rotations

A model has been developed which assesses the economic and environmental performance of crop rotations, in both conventional and organic cropping, for over 70 arable and horticultural crops, and a wide range of growing conditions in Europe. The model, though originally based on the N_ABLE model, has been completely rewritten and contains new routines to simulate root development, the mineralisation and release of nitrogen (N) from soil organic matter and crop residues, and water dynamics in soil. New routines have been added to estimate the effects of sub-optimal rates of N and spacing on the marketable outputs and gross margins. The model provides a mechanism for generating scenarios to represent a range of differing crop and fertiliser management strategies which can be used to evaluate their effects on yield, gross margin and losses of nitrogen through leaching. Such testing has revealed that nitrogen management can be improved and that there is potential to increase gross margins whilst reducing nitrogen losses

Ex‐post‐Evaluierung der Fördermaßnahmen BioChance und BioChancePlus im Rahmen der Systemevaluierung "KMU‐innovativ" : Begleit‐ und Wirkungsforschung zur Hightech‐Strategie

Der vorliegende Bericht stellt die Ergebnisse der Evaluation der BMBF-Fördermaßnahmen BioChance und BioChancePlus dar. Beide zielten als Vorläufer der Förderinitiative KMUinnovativ: Biotechnologie darauf ab, innovative und anspruchsvolle Forschungsvorhaben von kleinen und mittleren Unternehmen in der Biotechnologie zu ermöglichen. Die beiden Fördermaßnahmen unterschieden sich in Förderziel und Design: Während BioChance ab 1999 darauf abzielte, neu gegründete Firmen zu unterstützen, ging es in BioChancePlus ab 2003 darum, die weitere Entwicklung junger Biotechnologie-Unternehmen und deren risikoreichen Projekte voranzutreiben. Die vorliegende Analyse zeigt, dass BioChance und BioChancePlus eine angemessene Reaktion auf die Schwierigkeiten waren, mit denen die dedizierten Biotechnologie-Unternehmen in Deutschland in den Jahren 1999-2009 zu kämpfen hatten. Die Fördermaßnahmen wurden in ihrem Design jeweils adäquat weiterentwickelt und den Erfordernissen der Zielgruppe entsprechend angepasst. BioChance und BioChancePlus haben ihre Zielgruppen in hohem Maße erreicht: Von BioChance profitierten 15% der jungen Biotechnologiefirmen in Deutschland, der Nachfolger BioChancePlus erreichte 40% seiner Zielgruppe. Insgesamt erhielten 260 Unternehmen eine Zuwendung. 85% davon wurden nur einmal gefördert. Die öffentliche Förderung stellte eine wichtige, jedoch keineswegs die dominierende Finanzierungsquelle für die Unternehmen dar. So flossen im Zeitraum 2000-2009 rund 3 Mrd. Euro an VC-Investitionen in die Biotechnologie-Branche, während sich die öffentliche Förderung auf ca. 5% dieser Summe belief. Bei BioChance erhielten 17% der eingereichten Anträge eine Förderung, bei BioChancePlus waren es 29%. Insgesamt wurden durch die Maßnahme BioChance etwa 36 Millionen Euro und durch BioChancePlus 133 Millionen Euro an Fördergeldern gewährt

CHANGES IN VERTICAL JUMP PERFORMANCE AS A RESULT OF ALTERING THE FORCE-TIME CURVE TO EXHIBIT A SMOOTH RISE TO PEAK FORCE

INTRODUCTION Many individuals exhibit vertical jumping forcetime profiles that have multiple maxima. Based upon the discussions of Hochmuth (1984), the perfect force-time curve exhibited in a vertical jump, is one that exhibits an immediate rise to maximum force followed by a maintenance of maximum force for as long as possible until force application to the ground immediately ceases. Graphically, force with respect to time would resemble a square impulse. Physically this type of force production is impossible to generate by the human body. Pilot work examining force-time curves in vertical jumping indicated that some individuals generated smooth rises to a single maximum peak force. Continuing Hochmuth's argument, a smooth rise to a single peak force is most likely the best performance that one could exhibit. The exploratory question then becomes: Do smooth rises to peak force enhance performance? The purpose of this investigation was to examine the effects of altering existing force-time curves mathematically to produce a smooth rise to a single peak force. METHOD Smooth rises to peak force were attained by fitting a parabolic trajectory to the force record of 43 individuals. It was hypothesized that Effective Integration of the System (EIS) scores and vertical jump heights would improve as a result of fitting a parabolic rise to peak force. Variables with respect to time of jump, magnitude of maximum force, time of eccentric and concentric contractions were not altered. RESULTS AND CONCLUSIONS In every instance, EIS and vertical jump heights improved. EIS improved from 18.96% to 40.43% after creating a smooth rise to peak force. Vertical jump height improved from an average of 33.66cm to 50.08cm as a result of altering the forcetime profile. The result of manipulating force-time curves to exhibit a smooth rise to peak force resulted in improved performance (i.e., hypothetically) for all individuals (n=43). The prediction model for skillfulness also increased significantly E IS and vertical jump height following parabolic fitting. Admittedly, altering force-time curves to obtained a smooth rise to peak force does not offer significant insights into what to alter or how to alter movement in vertical jumping to elicit smooth rises to peak force. However, increased hypothetical performance measures certainly indicate that future investigations should examine other force-time patterns effects on performance and determine variables (e.g., in the kinematic record) that are conducive to assisting performers in producing smooth rises to maximum force application to the ground

Unsharp Quantum Reality

The positive operator (valued) measures (POMs) allow one to generalize the notion of observable beyond the traditional one based on projection valued measures (PVMs). Here, we argue that this generalized conception of observable enables a consistent notion of unsharp reality and with it an adequate concept of joint properties. A sharp or unsharp property manifests itself as an element of sharp or unsharp reality by its tendency to become actual or to actualize a specific measurement outcome. This actualization tendency-or potentiality-of a property is quantified by the associated quantum probability. The resulting single-case interpretation of probability as a degree of reality will be explained in detail and its role in addressing the tensions between quantum and classical accounts of the physical world will be elucidated. It will be shown that potentiality can be viewed as a causal agency that evolves in a well-defined way

The Clavier concertos of the eighteenth and early nineteenth centuries

Thesis (M.M.)--Boston Universit