23 research outputs found
Gastrin: A Distinct Fate of Neurogenin3 Positive Progenitor Cells in the Embryonic Pancreas
Neurogenin3+ (Ngn3+) progenitor cells in the developing pancreas give rise to five endocrine cell types secreting insulin, glucagon, somatostatin, pancreatic polypeptide and ghrelin. Gastrin is a hormone produced primarily by G-cells in the stomach, where it functions to stimulate acid secretion by gastric parietal cells. Gastrin is expressed in the embryonic pancreas and is common in islet cell tumors, but the lineage and regulators of pancreatic gastrin+ cells are not known. We report that gastrin is abundantly expressed in the embryonic pancreas and disappears soon after birth. Some gastrin+ cells in the developing pancreas co-express glucagon, ghrelin or pancreatic polypeptide, but many gastrin+ cells do not express any other islet hormone. Pancreatic gastrin+ cells express the transcription factors Nkx6.1, Nkx2.2 and low levels of Pdx1, and derive from Ngn3+ endocrine progenitor cells as shown by genetic lineage tracing. Using mice deficient for key transcription factors we show that gastrin expression depends on Ngn3, Nkx2.2, NeuroD1 and Arx, but not Pax4 or Pax6. Finally, gastrin expression is induced upon differentiation of human embryonic stem cells to pancreatic endocrine cells expressing insulin. Thus, gastrin+ cells are a distinct endocrine cell type in the pancreas and an alternative fate of Ngn3+ cells
Gastrin is abundantly expressed in the embryonic pancreas, and disappears after birth.
<p>A. Expression of gastrin in e12.5 mouse pancreas explanted for 3 days. The image was generated by merging 4 confocal slices (Z-stack). Scale bar = 100 µm. B. In vivo expression of gastrin throughout the pancreas of an e14.5 mouse embryo. The image was generated by merging 11 confocal slices (Z-stack). C. Quantification of gastrin and insulin expression in E14.5 mouse embryo, using flow cytometry after intracellular staining for hormones. X-axis shows relative intensity of flurophore labeling insulin, whereas the y axis shows relative intensity of flurophore labeling gastrin. 0.6% of the cells label gastrin, but not insulin (quadrant Q1), and 0.7% of the cells are labeled with insulin, but not gastrin (quadrant Q4). The 0.04% of cells showing double labeling likely reflects background. D. Expression of gastrin in the mouse pancreas at different developmental ages. All images were taken on a Nikon C1 confocal microscope at a 20× or 60× magnification. Scale bar, 100 µm.</p
Control of pancreatic β cell regeneration by glucose metabolism.
Recent studies revealed a surprising regenerative capacity of insulin-producing β cells in mice, suggesting that regenerative therapy for human diabetes could in principle be achieved. Physiologic β cell regeneration under stressed conditions relies on accelerated proliferation of surviving β cells, but the factors that trigger and control this response remain unclear. Using islet transplantation experiments, we show that β cell mass is controlled systemically rather than by local factors such as tissue damage. Chronic changes in β cell glucose metabolism, rather than blood glucose levels per se, are the main positive regulator of basal and compensatory β cell proliferation in vivo. Intracellularly, genetic and pharmacologic manipulations reveal that glucose induces β cell replication via metabolism by glucokinase, the first step of glycolysis, followed by closure of K(ATP) channels and membrane depolarization. Our data provide a molecular mechanism for homeostatic control of β cell mass by metabolic demand
Pancreatic gastrin cells derive from Ngn3+ endocrine progenitors.
<p>Co-staining for gastrin (red) and the progeny of Ngn3<sup>+</sup> cells (anti-GFP, green) in Ngn3-Cre;Rosa26-LSL-YFP e15.5 mice reveals that all gastrin<sup>+</sup> cells have passed through a Ngn3-expressing stage. Scale bar, 100 µm.</p
Transcription factors required for gastrin expression in the embryonic pancreas.
<p>A. No expression of gastrin in Ngn3<sup>−/−</sup> pancreata is shown by immunofluorescence staining and significant gastrin reduction by RT-PCR at e14.5. Densitometry measurement of gastrin RNA levels (imageJ) shows that gastrin expression in ngn3<sup>+/+</sup> pancreata is markedly reduced in ngn3 KO (from 41,893 to 1,592; arbitrary units, the same area was picked for both bands). B. No expression of gastrin in Nkx2.2<sup>−/−</sup> pancreata is shown by immunofluorescence staining. Microarray analysis shows reduced expression of gastrin RNA in Nkx2.2<sup>−/−</sup>, NeuroD1<sup>−/−</sup> and double knockout embryos at e14.5. Values are presented as mean ± SE. C. Reduced expression of gastrin in Ngn3<sup>−/−</sup> and Arx<sup>−/−</sup> but not in Pax4<sup>−/−</sup> embryos at e12.5 (microarray analysis). Values are presented as mean ± SE. D. Pax6<sup>−/−</sup> embryonic pancreata have reduced numbers of gastrin<sup>+</sup> glucagon<sup>+</sup> cells, but gastrin<sup>+</sup> glucagon− cells are still observed. Scale bar, 100 µm for all panels.</p
Presence of gastrin-expressing cells that do not stain for other islet hormones.
<p>Images in panels A–H are from E15.5 mouse embryos. Arrows indicate cells co-expressing gastrin and other hormones, whereas arrowheads represent cells that express only gastrin. A–E. Co-staining for gastrin and insulin (A) or somatostatin (B) reveals no overlap. Co-staining for gastrin and glucagon (C), ghrelin (D) and pancreatic polypeptide (E) reveals some overlap, but some gastrin positive cells are negative for the other hormones. F. Triple staining for glucagon (green), gastrin (red) and ghrelin (blue) reveals some cells that stain only for gastrin. G–H. Co-staining for gastrin (red) and a cocktail of antibodies against insulin, glucagon, somatostatin, pancreatic polypeptide and ghrelin (G, H, green) reveals some cells that stain only for gastrin. I. Staining for gastrin (DAKO) reveals some gastrin<sup>+</sup> cells that are negative for insulin and glucagon in e22 Psammomys obesus. J. Co-staining for gastrin (red) and a cocktail of antibodies against insulin, glucagon, somatostatin, pancreatic polypeptide and ghrelin (green) in e22 Psammomys obesus, reveals cells that stain only for gastrin. K. Quantification of cells expressing gastrin with glucagon, ghrelin, pancreatic polypeptide or all pancreatic hormenos as percentage of all cells expressing gastrin at E15.5 (at least 100 cells were counted from different pancreata (n>5)). Scale bar, 100 µm. Values are presented as mean ± SE.</p
Transcription factors expressed in gastrin<sup>+</sup> cells.
<p>Gastrin<sup>+</sup> cells in e15.5 pancreas express Nkx6.1, Nkx2.2 and lower levels of Pdx1. Yellow arrows mark the cells in the insets. Scale bar, 100 µm.</p