Multi-taxa neo-taphonomic analysis of bone remains from barn owl pellets and cross-validation of observations: a case study from Dominica (Lesser Antilles)

Paleo- and neo-taphonomic analyses of bone assemblages rarely consider all the occurring taxa in a single study and works concerning birds of prey as accumulators of microvertebrate bone remains mostly focus on small mammals such as rodents and soricomorphs. However, raptors often hunt and consume a large range of taxa, including vertebrates such as small mammals, fishes, amphibians, squamates and birds. Bone remains of all these taxonomic groups are numerous in many paleontological and archaeological records, especially in cave deposits. To better characterize the predators at the origin of fossil and sub-fossil microvertebrate accumulations and the taphonomic history of the deposit, it is thus mandatory to conduct global and multi-taxa taphonomic approaches. The aim of this study is to provide an example of such a global approach through the investigation of a modern bone assemblage from a sample of pellets produced by the Lesser Antillean Barn Owl (Tyto insularis) in the island of Dominica. We propose a new methodology that allows us to compare different taxa (rodents, bats, squamates and birds) and to experiment with a cross-validation process using two observers for each taxonomic group to test the reliability of the taphonomic observations.1. Introduction 2. Materials and Methods 2.1. Owl Pellets Sampling 2.2. Prey Identification 2.3. Taphonomic Analysis 2.3.1. Anatomical Representation 2.3.2. Fragmentation 2.3.3. Surface Modifications 2.3.4. Size/Weight Classes of Preys 2.4. Cross-Validation of Observations 3. Results 3.1. Faunal Spectrum 3.2. Anatomical Representation 3.3. Fragmentation 3.4. Modifications of Bone Surface 4. Discussion 4.1. Diet of Tyto Insularis in Dominica 4.2. Taphonomic Impact of Tyto Insularis on Small Vertebrate Bone Assemblage 4.2.1. Remarks on the Size/Weight Classes of Preys 4.2.2. Anatomical Representation 4.2.3. Fragmentation 4.2.4. Digestion 4.3. Degree of Inter-Observer Differences and Potential Outcomes 4.4. Towards an “Inter-Taxa Calibration” 5. Conclusion

The use of paleoclimatic simulations to refine the environmental and chronological context of archaeological/paleontological sites

To reconstruct the paleoenvironmental and chronological context of archaeological/paleontological sites is a key step to understand the evolutionary history of past organisms. Commonly used method to infer paleoenvironments rely on varied proxies such as faunal assemblages and isotopes. However, those proxies often show some inconsistencies. Regarding estimated ages of stratigraphic layers, they can vary depending on the dating method used. In this paper, we tested the potential of paleoclimate simulations to address this issue and contribute to the description of the environmental and chronological context of archaeological/paleontological sites. We produced a set of paleoclimate simulations corresponding to the stratigraphy of a Late-Pleistocene Holocene site, El Harhoura 2 (Morocco), and compared the climatic sequence described by these simulations to environmental inferences made from isotopes and faunal assemblages. Our results showed that in the studied site combined US-ESR ages were much more congruent with paleoenvironmental inferences than OSL ages. In addition, climatic variations were found to be more consistent with isotopic studies than faunal assemblages, allowing us to discuss unresolved discrepancies to date. This study illustrates the strong potential of our approach to refine the paleoenvironmental and chronological context of archaeological and paleontological sites.1 Introduction 2 Material and methods 2.1 El Harhoura 2 cave 2.2 Paleoclimate simulations 2.2.1 Pre-existing ensemble of simulations 2.2.2 Model 2.2.3 Sea-surface boundary conditions 2.3 Climate variations through EH2 sequence 3 Results 3.1 Paleoclimate simulations 3.2 Climate variations through EH2 sequence 4 Discussion 5 Conclusio

The use of paleoclimate simulations to refine the environmental and chronological context of archaeological/paleontological sites

This study illustrates the strong potential of combining paleoenvironmental reconstructions and paleoclimate modeling to refine the paleoenvironmental and chronological context of archaeologicaland paleontological sites. We focus on the El Harhoura 2 cave (EH2), an archeological site located on the North-Atlantic coast of Morocco that covers a period from the Late Pleistocene to the mid-Holocene. On several stratigraphic layers, inconsistencies are observed between species- and isotope-based inferences used to reconstruct paleoenvironmental conditions. The stratigraphy of EH2 also shows chronological inconsistencies on older layers between age estimated by Optical Stimulated Luminescence (OSL) and Combination of Uranium Series and Electron Spin Resonance methods (combined US-ESR). We performed paleoclimate simulations to infer the global paleoclimate variations over the EH2 sequence in the area, and we conducted a consistency approach between paleoclimatereconstruction estimated from simulations and available from EH2 paleoenvironmental inferences. Our main conclusion show that the climate sequence based on combined US-ESR ages is more consistent with paleoenvironmental inferences than the climate sequence based on OSL ages. We also evidence that isotope-based inferences are more congruent with the paleoclimate sequence than species-based inferences. These results highlight the difference in scale between the information provided by each ofthese paleoenvironmental proxies. Our approach is transferable to other sites due to the increase number of available paleoclimate simulations.1 Introduction 2 Material and methods 2.1 El Harhoura 2 cave 2.1.1 Presentation of the site 2.1.2 Chronostratigraphy and dating hypotheses 2.1.3 Paleoenvironmental variables 2.2 Paleoclimate reconstruction 2.2.1 Climate model 2.2.2 Paleoclimate simulations 2.2.3 Sea-surface boundary conditions 2.2.4 A subset of key paleoclimate variables 2.3 Consistency analyses 3 Results 3.1. Simulated climate changes 3.2 Consistency between paleoclimate simulations and paleoenvironmental inferences 3.2.1 Association of paleoclimate simulations and stratigraphic layers 3.2.2 Consistency analyses 4 Discussion 4.1 Paleoclimate variation and underlying forcings 4.2 Paleoclimate simulations and chronostratigraphy 4.3 Paleoclimate simulations and paleoenvironmental inferences 5 Conclusion

An improved chronology for the Middle Stone Age at El Mnasra cave, Morocco

North African coastal Middle Stone Age (MSA) sites are key to study the development and expansion of early H. sapiens. El Mnasra cave on the Atlantic coast of Morocco (Témara region) is a crucial site associated with MSA archaeological materials considered advanced cognitive hallmarks of behavioural innovation, such as numerous Nassariidae perforated shells, hematite pigments, bones industry and coastal resources exploitation. We provide new trapped-charges dates (OSL and combined US-ESR ages). Our Bayesian modelling strengthens the new lithostratigraphic interpretation of the cave stratigraphic units (US) and we propose an updated chronostratigraphic model for the Middle Stone Age archaeo-sequence of El Mnasra Cave. We confirm a human presence between 124–104 ka, earlier than what the previous OSL and US-ESR data showed. Our time range intervals allowed us to also extend the age of the MSA occupations considerably to the MIS 4/3 (~62–30 ka), marked by the disappearance of the Nassariidae perforated shells. Outstandingly, our model pushed back the age of the largest record of Nassariidae perforated shells and placed the age of their use by the Aterian groups at El Mnasra from the MIS 5d-5b (~115–94 ka)

Pacific Northwest National Laboratory Site Environmental Report for Calendar Year 2011

The PNNL Site Environmental Report for Calendar Year 2011 was prepared pursuant to the requirements of Department of Energy (DOE) Order 231.1B, "Environment, Safety and Health Reporting" to provide a synopsis of calendar year 2011 information related to environmental management performance and compliance efforts. It summarizes site compliance with federal, state, and local environmental laws, regulations, policies, directives, permits, and orders and environmental management performance

Limpet Shells from the Aterian Level 8 of El Harhoura 2 Cave (Témara, Morocco): Preservation State of Crossed-Foliated Layers

International audienceThe exploitation of mollusks by the first anatomically modern humans is a central question for archaeologists. This paper focuses on level 8 (dated around * 100 ka BP) of El Har-houra 2 Cave, located along the coastline in the Rabat-Témara region (Morocco). The large quantity of Patella sp. shells found in this level highlights questions regarding their origin and preservation. This study presents an estimation of the preservation status of these shells. We focus here on the diagenetic evolution of both the microstructural patterns and organic components of crossed-foliated shell layers, in order to assess the viability of further investigations based on shell layer minor elements, isotopic or biochemical compositions. The results show that the shells seem to be well conserved, with microstructural patterns preserved down to sub-micrometric scales, and that some organic components are still present in situ. But faint taphonomic degradations affecting both mineral and organic components are nonetheless evidenced, such as the disappearance of organic envelopes surrounding crossed-foliated lamellae, combined with a partial recrystallization of the lamellae. Our results provide a solid case-study of the early stages of the diagenetic evolution of crossed-foliated shell layers. Moreover, they highlight the fact that extreme caution must be taken before using fossil shells for palaeoenvironmental or geochronological reconstructions. Without thorough investigation, the alteration patterns illustrated here would easily have gone unnoticed. However, these degradations are liable to bias any proxy based on the elemental, isotopic or biochemical composition of the shells. This study also provides significant data concerning human subsistence behavior: the presence of notches and the good preservation state of limpet shells (no dissolution/recrystallization, no bioerosion and no abrasion/fragmentation aspects) would attest that limpets were gathered alive with tools by Middle Palaeolithic (Aterian) populations in North Africa for consumption

Gorab is a Golgi protein required for structure and duplication of Drosophila centrioles.

We demonstrate that a Drosophila Golgi protein, Gorab, is present not only in the trans-Golgi but also in the centriole cartwheel where, complexed to Sas6, it is required for centriole duplication. In addition to centriole defects, flies lacking Gorab are uncoordinated due to defects in sensory cilia, which lose their nine-fold symmetry. We demonstrate the separation of centriole and Golgi functions of Drosophila Gorab in two ways: first, we have created Gorab variants that are unable to localize to trans-Golgi but can still rescue the centriole and cilia defects of gorab null flies; second, we show that expression of C-terminally tagged Gorab disrupts Golgi functions in cytokinesis of male meiosis, a dominant phenotype overcome by mutations preventing Golgi targeting. Our findings suggest that during animal evolution, a Golgi protein has arisen with a second, apparently independent, role in centriole duplication.D.M.G. is grateful for a Wellcome Investigator Award, which supported this work. The study was initiated with support from Cancer Research UK

Zebrafish: a vertebrate tool for studying basal body biogenesis, structure, and function.

Understanding the role of basal bodies (BBs) during development and disease has been largely overshadowed by research into the function of the cilium. Although these two organelles are closely associated, they have specific roles to complete for successful cellular development. Appropriate development and function of the BB are fundamental for cilia function. Indeed, there are a growing number of human genetic diseases affecting ciliary development, known collectively as the ciliopathies. Accumulating evidence suggests that BBs establish cell polarity, direct ciliogenesis, and provide docking sites for proteins required within the ciliary axoneme. Major contributions to our knowledge of BB structure and function have been provided by studies in flagellated or ciliated unicellular eukaryotic organisms, specifically Tetrahymena and Chlamydomonas. Reproducing these and other findings in vertebrates has required animal in vivo models. Zebrafish have fast become one of the primary organisms of choice for modeling vertebrate functional genetics. Rapid ex-utero development, proficient egg laying, ease of genetic manipulation, and affordability make zebrafish an attractive vertebrate research tool. Furthermore, zebrafish share over 80 % of disease causing genes with humans. In this article, we discuss the merits of using zebrafish to study BB functional genetics, review current knowledge of zebrafish BB ultrastructure and mechanisms of function, and consider the outlook for future zebrafish-based BB studies