Surgery From an Osteopathic Standpoint

This 1904 treasure, Surgery from the Osteopathic Standpoint, follows Andrew T. Still’s essential facts of practical surgery as modified by Osteopathy. While operative methods are not detailed, it is beautifully illustrated by students at the American School of Osteopathy. Topics covered include inflammation, tuberculosis, cysts, wounds, shock, dislocations, fractures, and diseases and injuries of the spine, head, mouth, chest, and digestive tract. Most interesting are the passages on antiseptics and Tetanus which are of historical relevancy.https://digitalcommons.pcom.edu/classic_med_works/1008/thumbnail.jp

XMM-Newton detection of Nova Muscae 1991 in Quiescence

The soft X-ray transient GU Mus has been detected by XMM-Newton in the quiescent state. The source is very faint, with a 0.5-10.0 keV unabsorbed flux of $\simeq 1.1 \times 10^{-14}$ ergs cm$^{-2}$ s$^{-1}$. The spectra is well fit by an absorbed powerlaw with a photon index of $\alpha = 1.6 \pm 0.4$, close to the value seen when the source was in the low/hard state in Aug. 1991. From our observed luminosity, it seems unlikely that the quiescent state emission is dominated by coronal X-rays from the secondary. The flux also appears to be in agreement with the ADAF model of BH-transients in quiescence.Comment: 6 pages including 3 figures. Accepted for publication, Astronomy and Astrophysic

Swift J1753.5-0127: The Black Hole Candidate with the shortest orbital period

We present time-resolved photometry of the optical counterpart to the black hole candidate Swift J1753.5-0127, which has remained in the low/hard X-ray state and bright at optical/IR wavelengths since its discovery in 2005. At the time of our observations Swift J1753.5-0127 does not show a decay trend but remains stable at R=16.45 with a night to night variability of ~0.05 mag. The R-band light curves, taken from 2007 June 3 to August 31, are not sinusoidal, but exhibit a complex morphology with remarkable changes in shape and amplitude. The best period determination is 3.2443+-0.0010 hours. This photometric period is likely a superhump period, slightly larger than the orbital period. Therefore, Swift J1753.5-0127 is the black hole candidate with the shortest orbital period observed to date. Our estimation of the distance is comparable to values previously published and likely places Swift J1753.5-0127 in the Galactic halo.Comment: Accepted for publication in Ap

In Silico and In Vitro Investigations of the Mutability of Disease-Causing Missense Mutation Sites in Spermine Synthase

Spermine synthase (SMS) is a key enzyme controlling the concentration of spermidine and spermine in the cell. The importance of SMS is manifested by the fact that single missense mutations were found to cause Snyder-Robinson Syndrome (SRS). At the same time, currently there are no non-synonymous single nucleoside polymorphisms, nsSNPs (harmless mutations), found in SMS, which may imply that the SMS does not tolerate amino acid substitutions, i.e. is not mutable.To investigate the mutability of the SMS, we carried out in silico analysis and in vitro experiments of the effects of amino acid substitutions at the missense mutation sites (G56, V132 and I150) that have been shown to cause SRS. Our investigation showed that the mutation sites have different degree of mutability depending on their structural micro-environment and involvement in the function and structural integrity of the SMS. It was found that the I150 site does not tolerate any mutation, while V132, despite its key position at the interface of SMS dimer, is quite mutable. The G56 site is in the middle of the spectra, but still quite sensitive to charge residue replacement.The performed analysis showed that mutability depends on the detail of the structural and functional factors and cannot be predicted based on conservation of wild type properties alone. Also, harmless nsSNPs can be expected to occur even at sites at which missense mutations were found to cause diseases

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead