17 research outputs found
Cross talk between IGF-I receptor signalling and the sphingomyelin pathway during ceramide-induced apoptosis
Increased, not decreased activation of the insulin-like growth factor (IGF) receptor signalling pathway during ceramide-induced apoptosis
Effective formation of MHC class II peptide complexes from endogenous antigen by thyroid epithelial cells
Human growth disorders: molecular genetics of the growth hormone–insulin-like growth factor I axis
Subcutaneous and visceral preadipocytes from pre-pubertal children show site-specific differences in insulin action
Depot-specific effects of fatty acids on lipid accumulation in children’s adipocytes
Circulating concentrations of fatty acids are elevated in obesity, although their effect on regional fat deposition is relatively unexplored. With the increasing prevalence of childhood obesity, we aimed to investigate whether saturated and unsaturated fatty acids lead to differential lipid accumulation (LA) in children’s subcutaneous and visceral adipocytes. To examine this, subcutaneous and peri-nephric pre-adipocytes, isolated from fat biopsies from 6 pre-pubertal children, were differentiated in vitro before being exposed to palmitate and/or oleate for 24 h. Lipid accumulation was then quantified by nile red staining. Palmitate significantly increased LA in visceral adipocytes at all doses 188 μM (e.g. Palmitate 750 μM: +30.0%[8.2]; p < 0.01), whilst only a dose of 375 μM led to a significant, but smaller, increase in LA in subcutaneous adipocytes (Palmitate 375 μM: +13.0%[4.3]; p = 0.02). In contrast, oleate significantly increased LA in subcutaneous (Oleate 1000 μM: +36.3%[14.0]; p = 0.01), but not visceral (Oleate 1000 μM: +16.2%[9.6]; p = 0.25) adipocytes. These data suggest that saturated and unsaturated fatty acids may exert depot-specific effects on lipid accumulation