Modelling the impact of wastewater flows and management practices on antimicrobial resistance in dairy farms

Dairy slurry is a major source of environmental contamination with antimicrobial resistant genes and bacteria. We developed mathematical models and conducted on-farm research to explore the impact of wastewater flows and management practices on antimicrobial resistance (AMR) in slurry. Temporal fluctuations in cephalosporin-resistant Escherichia coli were observed and attributed to farm activities, specifically the disposal of spent copper and zinc footbath into the slurry system. Our model revealed that resistance should be more frequently observed with relevant determinants encoded chromosomally rather than on plasmids, which was supported by reanalysis of sequenced genomes from the farm. Additionally, lower resistance levels were predicted in conditions with lower growth and higher death rates. The use of muck heap effluent for washing dirty channels did not explain the fluctuations in cephalosporin resistance. These results highlight farm-specific opportunities to reduce AMR pollution, beyond antibiotic use reduction, including careful disposal or recycling of waste antimicrobial metals

Modelling the impact of wastewater flows and management practices on antimicrobial resistance in dairy farms

Dairy slurry is a major source of environmental contamination with antimicrobial resistant genes and bacteria. We developed mathematical models and conducted on-farm research to explore the impact of wastewater flows and management practices on antimicrobial resistance (AMR) in slurry. Temporal fluctuations in cephalosporin-resistant Escherichia coli were observed and attributed to farm activities, specifically the disposal of spent copper and zinc footbath into the slurry system. Our model revealed that resistance should be more frequently observed with relevant determinants encoded chromosomally rather than on plasmids, which was supported by reanalysis of sequenced genomes from the farm. Additionally, lower resistance levels were predicted in conditions with lower growth and higher death rates. The use of muck heap effluent for washing dirty channels did not explain the fluctuations in cephalosporin resistance. These results highlight farm-specific opportunities to reduce AMR pollution, beyond antibiotic use reduction, including careful disposal or recycling of waste antimicrobial metals

A suggested new bacteriophage genus: “Viunalikevirus”

We suggest a bacteriophage genus, “Viunalikevirus”, as a new genus within the family Myoviridae. To date, this genus includes seven sequenced members: Salmonella phages ViI, SFP10 and ΦSH19; Escherichia phages CBA120 and PhaxI; Shigella phage phiSboM-AG3; and Dickeya phage LIMEstone1. Their shared myovirus morphology, with comparable head sizes and tail dimensions, and genome organization are considered distinguishing features. They appear to have conserved regulatory sequences, a horizontally acquired tRNA set and the probable substitution of an alternate base for thymine in the DNA. A close examination of the tail spike region in the DNA revealed four distinct tail spike proteins, an arrangement which might lead to the umbrella-like structures of the tails visible on electron micrographs. These properties set the suggested genus apart from the recently ratified subfamily Tevenvirinae, although a significant evolutionary relationship can be observed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00705-012-1360-5) contains supplementary material, which is available to authorized users

Modelling the impact of wastewater flows and management practices on antimicrobial resistance in dairy farms

Dairy slurry is a major source of environmental contamination with antimicrobial resistant genes and bacteria. We developed mathematical models and conducted on-farm research to explore the impact of wastewater flows and management practices on antimicrobial resistance (AMR) in slurry. Temporal fluctuations in cephalosporin-resistant Escherichia coli were observed and attributed to farm activities, specifically the disposal of spent copper and zinc footbath into the slurry system. Our model revealed that resistance should be more frequently observed with relevant determinants encoded chromosomally rather than on plasmids, which was supported by reanalysis of sequenced genomes from the farm. Additionally, lower resistance levels were predicted in conditions with lower growth and higher death rates. The use of muck heap effluent for washing dirty channels did not explain the fluctuations in cephalosporin resistance. These results highlight farm-specific opportunities to reduce AMR pollution, beyond antibiotic use reduction, including careful disposal or recycling of waste antimicrobial metals. Antimicrobial resistance (AMR) is one of the most important global public health problems. It is estimated that 1.27 million deaths were attributed to AMR bacteria globally in 2019 1 , and, unless suitable countermeasures are taken, that number is predicted to rise to 10 million by 2050 2. AMR is driven by antibiotic use; the majority (73%) of antibiotic (Ab) sales are for use for food-producing livestock 3. The use of Abs in agriculture can result in drug-resistant strains infecting human populations through the food chain 4,5 , or may lead to the transfer of antibiotic resistance genes (ARGs) from livestock-associated bacteria to human-acquired infections 6-8. The importance of mitigating the risks of AMR in the agricultural sector has been recognised by many countries, including the UK, the European Union and the UN 2,9 , with reductions and restrictions being imposed on Ab use in agriculture, particularly on human critical antibiotics. However, despite a 55% reduction in Ab use in the UK agriculture sector since 2014 10 , use remains high, representing 36% of the total UK Ab use 11 , with consequent risk of spread of ARGs and AMR. In addition to antibiotics, other antimicrobials such as metals (copper and zinc) and other chemicals (e.g., formalin, disinfectants) are widely used across farms globally, particularly in footbaths to prevent lameness in livestock-a prevalent concern in dairy and sheep farming 12. Metals and other antimicrobial agents (such as formalin and glutaraldehyde) are known to have a co-selective effect on antibiotic resistance, allowing for th

Three new brown dwarfs and a massive hot Jupiter revealed by TESS around early-type stars

Context. The detection and characterization of exoplanets and brown dwarfs around massive AF-type stars is essential to investigate and constrain the impact of stellar mass on planet properties. However, such targets are still poorly explored in radial velocity (RV) surveys because they only feature a small number of stellar lines and those are usually broadened and blended by stellar rotation as well as stellar jitter. As a result, the available information about the formation and evolution of planets and brown dwarfs around hot stars is limited. Aims. We aim to increase the sample and precisely measure the masses and eccentricities of giant planets and brown dwarfs transiting early-type stars detected by the Transiting Exoplanet Survey Satellite (TESS). Methods. We followed bright (V 6200 K that host giant companions (R > 7 R⊕) using ground-based photometric observations as well as high precision radial velocity measurements from the CORALIE, CHIRON, TRES, FEROS, and MINERVA-Australis spectrographs. Results. In the context of the search for exoplanets and brown dwarfs around early-type stars, we present the discovery of three brown dwarf companions, TOI-629b, TOI-1982b, and TOI-2543b, and one massive planet, TOI-1107b. From the joint analysis of TESS and ground-based photometry in combination with high precision radial velocity measurements, we find the brown dwarfs have masses between 66 and 68 MJup, periods between 7.54 and 17.17 days, and radii between 0.95 and 1.11 RJup. The hot Jupiter TOI-1107b has an orbital period of 4.08 days, a radius of 1.30 RJup, and a mass of 3.35 MJup. As a by-product of this program, we identified four low-mass eclipsing components (TOI-288b, TOI-446b, TOI-478b, and TOI-764b). Conclusions. Both TOI-1107b and TOI-1982b present an anomalously inflated radius with respect to the age of these systems. TOI-629 is among the hottest stars with a known transiting brown dwarf. TOI-629b and TOI-1982b are among the most eccentric brown dwarfs. The massive planet and the three brown dwarfs add to the growing population of well-characterized giant planets and brown dwarfs transiting AF-type stars and they reduce the apparent paucity

Metagenomics of the viral community in three cattle slurry samples

The diversity of viruses in slurries from dairy farming remains largely uncharacterized. Here we report viral diversity found in cattle slurry from a dairy farm in the East Midlands in the United Kingdom. The same slurry tank was sampled in three consecutive years, and the viral fraction was isolated and sequenced

TESS and CHEOPS discover two warm sub-Neptunes transiting the bright K-dwarf HD 15906

We report the discovery of two warm sub-Neptunes transiting the bright (G = 9.5 mag) K-dwarf HD 15906 (TOI 461, TIC 4646810). This star was observed by the Transiting Exoplanet Survey Satellite (TESS) in sectors 4 and 31, revealing two small transiting planets. The inner planet, HD 15906 b, was detected with an unambiguous period but the outer planet, HD 15906 c, showed only two transits separated by ∼ 734 d, leading to 36 possible values of its period. We performed follow-up observations with the CHaracterising ExOPlanet Satellite (CHEOPS) to confirm the true period of HD 15906 c and improve the radius precision of the two planets. From TESS, CHEOPS, and additional ground-based photometry, we find that HD 15906 b has a radius of 2.24 ± 0.08 R and a period of 10.924709 ± 0.000032 d, whilst HD 15906 c has a radius of 2.93+−000607 R and a period of 21.583298+−00000055000052 d. Assuming zero bond albedo and full day-night heat redistribution, the inner and outer planet have equilibrium temperatures of 668 ± 13 K and 532 ± 10 K, respectively. The HD 15906 system has become one of only six multiplanet systems with two warm (700 K) sub-Neptune sized planets transiting a bright star (G ≤ 10 mag). It is an excellent target for detailed characterization studies to constrain the composition of sub-Neptune planets and test theories of planet formation and evolution.ISSN:0035-8711ISSN:1365-296