7,832 research outputs found
The C. Elegans ROR receptor tyrosine kinase, CAM-1, non-autonomously inhibits the Wnt pathway
Inhibitors of Wnt signaling promote normal development and prevent cancer by restraining when and where the Wnt pathway is activated. ROR proteins, a class of Wnt-binding receptor tyrosine kinases, inhibit Wnt signaling by an unknown mechanism. To clarify how RORs inhibit the Wnt pathway, we examined the relationship between Wnts and the sole C. elegans ROR homolog, cam-1, during C. elegans vulval development, a Wnt-regulated process. We found that loss and overexpression of cam-1 causes reciprocal defects in Wnt-mediated cell-fate specification. Our molecular and genetic analyses revealed that the CAM-1 extracellular domain (ECD) is sufficient to non-autonomously antagonize multiple Wnts, suggesting that the CAM-1/ROR ECD sequesters Wnts. A sequestration model is supported by our findings that the CAM-1 ECD binds to several Wnts in vitro. These results demonstrate how ROR proteins help to refine the spatial pattern of Wnt activity in a complex multicellular environment
Sensitive and Precise Quantification of Insulin-Like mRNA Expression in Caenorhabditis elegans
Insulin-like signaling regulates developmental arrest, stress resistance and lifespan in the nematode Caenorhabditis elegans. However, the genome encodes 40 insulin-like peptides, and the regulation and function of individual peptides is largely uncharacterized. We used the nCounter platform to measure mRNA expression of all 40 insulin-like peptides as well as the insulin-like receptor daf-2, its transcriptional effector daf-16, and the daf-16 target gene sod-3. We validated the platform using 53 RNA samples previously characterized by high density oligonucleotide microarray analysis. For this set of genes and the standard nCounter protocol, sensitivity and precision were comparable between the two platforms. We optimized conditions of the nCounter assay by varying the mass of total RNA used for hybridization, thereby increasing sensitivity up to 50-fold and reducing the median coefficient of variation as much as 4-fold. We used deletion mutants to demonstrate specificity of the assay, and we used optimized conditions to assay insulin-like gene expression throughout the C. elegans life cycle. We detected expression for nearly all insulin-like genes and find that they are expressed in a variety of distinct patterns suggesting complexity of regulation and specificity of function. We identified insulin-like genes that are specifically expressed during developmental arrest, larval development, adulthood and embryogenesis. These results demonstrate that the nCounter platform provides a powerful approach to analyzing insulin-like gene expression dynamics, and they suggest hypotheses about the function of individual insulin-like genes
Recommended from our members
Mentes Positivas en Acción: Feasibility Study of a Promotor-Delivered Cognitive Behavioral Stress Management Program for Low-Income Spanish-Speaking Latinas.
Purpose: Low-income Latino immigrants lack access to mental health providers. We explored the feasibility of training promotores to deliver a stress management program in community settings. Methods: We trained promotores to deliver an 8-week intervention program comprising evidence-based cognitive-behavioral stress management techniques. Trained promotores then delivered the program to Spanish-speaking Latino immigrants. Results: Promotores (n=10) improved their knowledge significantly after the training (p<0.001) and delivered the program demonstrating excellent fidelity. Participants who received the program (n=50) had significantly improved scores on immigration stress, perceived stress, and depressive symptoms (p<0.001). Conclusion: It is feasible to train Latino promotores to deliver an effective stress management program to low-income Latino immigrants in their communities. Results contribute to a growing literature on the value of such interventions in community settings. If it is found to be effective in future studies, the program could help fill a large need in the Latino community
Eddington limited starbursts in the central 10pc of AGN, and the Torus in NGC1068
We present results from a survey of nearby AGN using the near infrared
adaptive optics integral field spectrograph SINFONI. These data enable us to
probe the distribution and kinematics of the gas and stars at spatial
resolutions as small as 0.085arcsec. We find strong evidence for recent but
short lived starbursts residing in very dense nuclear disks. On scales of less
than 10pc these would have reached Eddington-limited luminosities when active,
perhaps accounting for their short duration. In addition, for NGC1068 at a
resolution of 6pc, we present direct observations of molecular gas close around
the AGN which we identify with the obscuring torus.Comment: Conference proceedings to appear in "The Central Engine of Active
Galactic Nuclei", ed. L. C. Ho and J.-M. Wang (San Francisco: ASP
Modulation of EGF receptor-mediated vulva development by the heterotrimeric G-protein G{alpha}q and excitable cells in C. elegans
Issues in the Design of a Pilot Concept-Based Query Interface for the Neuroinformatics Information Framework
This paper describes a pilot query interface that has been constructed to help us explore a "concept-based" approach for searching the
Neuroscience Information Framework (NIF). The query interface is
concept-based in the sense that the search terms submitted through the
interface are selected from a standardized vocabulary of terms
(concepts) that are structured in the form of an ontology. The NIF
contains three primary resources: the NIF Resource Registry, the NIF
Document Archive, and the NIF Database Mediator. These NIF resources
are very different in their nature and therefore pose challenges when
designing a single interface from which searches can be automatically
launched against all three resources simultaneously. The paper first
discusses briefly several background issues involving the use of
standardized biomedical vocabularies in biomedical information
retrieval, and then presents a detailed example that illustrates how
the pilot concept-based query interface operates. The paper concludes
by discussing certain lessons learned in the development of the current
version of the interface
Central Nervous System Parasitosis and Neuroinflammation Ameliorated by Systemic IL-10 Administration in Trypanosoma brucei-Infected Mice
Peer reviewedPublisher PD
Local trace formulae and scaling asymptotics in Toeplitz quantization
A trace formula for Toeplitz operators was proved by Boutet de Monvel and
Guillemin in the setting of general Toeplitz structures. Here we give a local
version of this result for a class of Toeplitz operators related to continuous
groups of symmetries on quantizable compact symplectic manifolds. The local
trace formula involves certain scaling asymptotics along the clean fixed locus
of the Hamiltonian flow of the symbol, reminiscent of the scaling asymptotics
of the equivariant components of the Szeg\"o kernel along the diagonal
- …