EPR detection of phytophenoxyl radicals stabilized by zinc ions: evidence for the redox coupling of plant phenolics with ascorbate in the H2O2-peroxidase system

AbstractChlorogenic acid (CGA; 3-o-caffeoylquinic acid), a phenylpropanoid metabolite of plants, was oxidized by H2O2 in the presence of horseradish peroxidase. The primary and secondary oxidized products both were free radicals which gave EPR multiline signals at g=2.0044 and 2.0042 in the presence of zinc as a spin stabilizing agent. The EPR kinetics showed that ascorbate functioned as a cooperative reductant by regenerating CGA from its corresponding radicals. These results provide evidence to support the idea that the ascorbate-phenolic redox couple in conjunction with guaiacol peroxidase is an efficient H2O2 scavenging mechanism in higher plants

Processing and Visualization of Metabolomics Data Using R

Metabolomics provides a wealth of information about the biochemical status of cells, tissues, and other biological systems. However, for many researchers, processing the large quantities of data generated in typical metabolomics experiments poses a formidable challenge. Robust computational tools are required for all data processing steps, from handling raw data to high level statistical analysis and interpretation. This chapter describes several established methods for processing and analyzing metabolomics data within the R statistical programming environment. The focus is on processing LCMS data but the methods can be applied virtually to any analytical platform. We provide a step-by-step workflow to demonstrate how to integrate, analyze, and visualize LCMS-based metabolomics data using computational tools available in R. These concepts and methods will allow specialists and nonspecialists alike to develop and evaluate their own data more critically

The Effect of Foam Rolling Duration on Hamstring Range of Motion

Musculoskeletal health benefits from flexibility training and maintaining a functional, or sport specific, range of motion is important to one’s overall fitness. Commercial foam rollers are commonly used in gyms, therapy clinics and homes, yet data are lacking on the optimal rolling duration and effect on range of motion

Extension Assistant Professor State Specialist—Community Development Process

research rural economic and historical factors that affect health insurance availability, including cost drivers and barriers. A team of academics, health care professionals and citizens are examining the history of the health system and economy in this rural county of 12,000, halfway between St. Louis and Columbia. This project presents an opportunity to understand the tremendous transition in rural health in the last century and how the changing rural economy affects coverage. The vehicle of community-directed documentary filmmaking is being used (1) to engage the community in its own self-analysis and exploration of this complex issue; (2) provide the community with a medium for communication; and (3) share project results. This document provides information on community-directed filmmaking as a community development tool and on the Montgomery County pilot project. Key words: community development tool; community video; film; health; participatory researchCOMMUNITY VIDEO AS A COMMUNITY DEVELOPMENT TOOL Movie production, once limited to Hollywood professionals or home movie amateurs, has become increasingly common in the last decade with the advent of improved, high quality, consumer-level video cameras and affordable digital video editing programs. Children across the country are making videos as part of classroom projects, and college students are producin

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Draft Genome of Bugula neritina, a Colonial Animal Packing Powerful Symbionts and Potential Medicines

Many animal phyla have no representatives within the catalog of whole metazoan genome sequences. This dataset fills in one gap in the genome knowledge of animal phyla with a draft genome of Bugula neritina (phylum Bryozoa). Interest in this species spans ecology and biomedical sciences because B. neritina is the natural source of bioactive compounds called bryostatins. Here we present a draft assembly of the B. neritina genome obtained from PacBio and Illumina HiSeq data, as well as genes and proteins predicted de novo and verified using transcriptome data, along with the functional annotation. These sequences will permit a better understanding of host-symbiont interactions at the genomic level, and also contribute additional phylogenomic markers to evaluate Lophophorate or Lophotrochozoa phylogenetic relationships. The effort also fits well with plans to ultimately sequence all orders of the Metazoa

Increased posterior cingulate functional connectivity following 6-month high-dose B-vitamin multivitamin supplementation : a randomized, double-blind, placebo-controlled trial

B vitamins are essential for optimal brain and body function, and are particularly important for cortical metabolic processes that have downstream effects on mitigating oxidative stress. Oxidative stress has been linked to poor psychological outcomes including psychological distress, which has wide-reaching implications for the community and the workplace. Given work-related stress has been associated with poor mental health outcomes, high-dose B vitamin supplementation may be effective in improving brain function and psychological outcomes via attenuation of oxidative stress. This randomized, double-blind, placebo-controlled study investigated psychological outcomes following 6-month supplementation of a high-B-vitamin multivitamin in a large sample of healthy adults (n = 108, aged 30–70 years), as well as changes in default mode network functional connectivity in a subset of the original sample (n = 28). Improvements in occupational stress, general health, perceived stress, depressive symptoms, and mood profiles were identified for both active and placebo groups over time (p < 0.05 corrected). Seed-based functional connectivity analysis centered on the posterior cingulate cortex (PCC) showed that connectivity between the PCC and the caudate increased for the active treatment group, but decreased for the placebo group (p < 0.05 corrected). These findings reveal a substantial intervention effect for both active and placebo treatments, which could in part be associated with a placebo effect in subjective measures. There was, however, a significant treatment effect in the objective measure of functional connectivity, suggesting that reduced psychological stress and high-B-vitamin multivitamin supplementation may lead to an increase in DMN and caudate functional connectivity, which might reflect a strengthening of neurocircuitry within areas associated with reward and emotion at rest. Future studies should consider a placebo run-in methodology to reduce the placebo effect on the subjective measures of stress

Increased posterior cingulate functional connectivity following 6-month high-dose B-vitamin multivitamin supplementation : a randomized, double-blind, placebo-controlled trial

B vitamins are essential for optimal brain and body function, and are particularly important for cortical metabolic processes that have downstream effects on mitigating oxidative stress. Oxidative stress has been linked to poor psychological outcomes including psychological distress, which has wide-reaching implications for the community and the workplace. Given work-related stress has been associated with poor mental health outcomes, high-dose B vitamin supplementation may be effective in improving brain function and psychological outcomes via attenuation of oxidative stress. This randomized, double-blind, placebo-controlled study investigated psychological outcomes following 6-month supplementation of a high-B-vitamin multivitamin in a large sample of healthy adults (n = 108, aged 30–70 years), as well as changes in default mode network functional connectivity in a subset of the original sample (n = 28). Improvements in occupational stress, general health, perceived stress, depressive symptoms, and mood profiles were identified for both active and placebo groups over time (p < 0.05 corrected). Seed-based functional connectivity analysis centered on the posterior cingulate cortex (PCC) showed that connectivity between the PCC and the caudate increased for the active treatment group, but decreased for the placebo group (p < 0.05 corrected). These findings reveal a substantial intervention effect for both active and placebo treatments, which could in part be associated with a placebo effect in subjective measures. There was, however, a significant treatment effect in the objective measure of functional connectivity, suggesting that reduced psychological stress and high-B-vitamin multivitamin supplementation may lead to an increase in DMN and caudate functional connectivity, which might reflect a strengthening of neurocircuitry within areas associated with reward and emotion at rest. Future studies should consider a placebo run-in methodology to reduce the placebo effect on the subjective measures of stress