Regulation of Adipocyte Transcription by PPARgamma Ligands

Rosiglitazone (rosi) is a powerful insulin sensitizer, but serious toxicities have curtailed its widespread clinical use. Rosi functions as a high-affinity ligand for PPARgamma, the adipocyte-predominant nuclear receptor (NR). The classic model of NR action, involving binding of ligand to the NR on DNA, explains positive regulation of gene expression, but both ligand-dependent transcriptional repression and indirect regulation are not well understood. We have addressed these issues by studying the direct effects of rosiglitazone on gene transcription, using global run-on sequencing (GRO-seq). Rosi-induced changes in gene body transcription were pronounced after 10 minutes and correlated with steady-state mRNA levels as well as with transcription at nearby enhancers (eRNAs). Up-regulated eRNAs occurred almost exclusively at PPARg binding sites, to which rosi treatment recruited coactivators including MED1, p300, and CBP, without changes in binding of the corepressor NCoR. By contrast, down-regulated eRNAs fell in sites devoid of PPARg but enriched for a variety of other TFs in the C/EBP and AP-1 families. These enhancers lost coactivator binding upon rosi treatment, suggesting that rosi treatment causes redistribution of coactivators to PPARg sites and away from enhancers containing other TFs, leading to transcriptional repression at these eRNAs and their target genes. We also investigated the function of MRL-24, a compound that has been shown to lack PPARg transactivation activity and regulate a distinct subset of PPARg target genes while functioning as an equally effective insulin sensitizer as rosi. Though our goal was to identify whether MRL-24 regulates the same functional enhancers marked by eRNAs as rosi, we instead found that MRL-24 does not control a distinct subset of target genes, but rather acts as a partial agonist for PPARg. Together, these studies further our understanding of transcriptional regulation by modulation of PPARg activity, including insights into determining functional enhancers and mechanisms of transcriptional repression by activation of a NR

Dynamic Quality of Service in Software-Defined Networks

Quality of service is a necessary function of today’s networks. A proper quality of service ensures that packets are delivered effectively and fast. In traditional networks, quality of service has to be manually configured on each piece of hardware in the network. This manual procedure makes the process of implementing a quality of service in a network costly. Not to mention, if part of the configuration is incorrect, or a mistake is made during the configuration, everything must be corrected on each piece of affected hardware. In this paper, I will be exploring the effect of using a software-defined network controller and a quality of service to handle certain flows of traffic in a network. The main tool used is the OpenFlow defined queue. Queues and flow rules will allow a switch to control individual flows and the network resources that each flow consumes. Factors that will be explored are the bandwidth usage of a flow and the time taken by the network to implement new flow rules. While bandwidth usage is taken into account in traditional networks, changing a quality of service is a new dynamic

Second consultation on developing standards for identifying evidence-based practices in reproductive health

The STEP UP consortium and the High Impact Practices initiative, together with other partners, supported three consultation meetings to inform the use of appropriate standards of evidence for recommending best practices in reproductive health (RH). The second consultation, reported here, brought together researchers and funders to review the research designs and methodologies that can be used to generate evidence on the impact of FP/RH interventions and on their implementation; the mechanisms and structures through which such evidence is reviewed and translated into recommendations; and the implications for organizing and funding evidence generation to maximize its quality and utility. Drawing from the presentations, three plenary discussions were held which reflected a diversity of views on what constitutes high-quality evidence, which type of evidence is appropriate for which type of recommendations, and which approaches should be followed to translate evidence into practice recommendations. Recommendations for consolidating standards of evidence and formulating guidelines for high-impact practices in FP/RH programming and policymaking are presented in this report

Village of Cuba St. Francis of Assisi Park Improvement Plan Community Input Report

At the request of the Village of Cuba Council, Healthy Kids, Healthy Cuba and Step into Cuba have gathered additional community input regarding the proposed improvements to the Village of Cuba St Francis of Assisi Park. Previously, we had obtained community input through a series of meetings held in Cuba. Chance Munns, a landscape architect working with the University of New Mexico Prevention Research Center, developed the community ideas into three maps with descriptions of the proposed improvements. At the request of the Village Council last fall, we obtained additional input from the Cuba community on the Park improvement plan via a mobile display placed throughout Cuba in the Spring and Summer of 2012

Leveraging Health Behavior and Communication Theories to Support Adolescent and Young Adults: Conceptualizing Social Media Wellness in Relation to Disordered Eating

Social media platforms like Instagram serve as an important mechanism for transmitting social information and influence. However, the nature and use of these platforms are known to perpetuate eating disorders (EDs) or further disorder eating symptoms. This concept paper proposes merging health behavior and communication theory to create a comprehensive and applicable framework for remediating pro-eating disorder social media content among people who have eating disorders. To this end, the Social Media Wellness Model, which is adapted from the Health Belief Model, the Uses and Gratifications approach, the MAIN model of media affordances, and media literacy training, is proposed. This paper shows how theoretical model components can be mapped back to behaviors typified by individuals with EDs, or those predisposed to developing EDs. Subsequently, we propose a training intervention to highlight salient literacy cues, and outline next steps for testing and developing this model with the ongoing support of a community advisory board (CAB). Creating a CAB with individuals who have lived experience of an ED or disordered eating, and who use social media, will be vital to testing the applicability of this conceptual Social Media Wellness Model for ED recovery

Dynamic Quality of Service in Software-Defined Networks

Quality of service is a necessary function of today’s networks. A proper quality of service ensures that packets are delivered effectively and fast. In traditional networks, quality of service has to be manually configured on each piece of hardware in the network. This manual procedure makes the process of implementing a quality of service in a network costly. Not to mention, if part of the configuration is incorrect, or a mistake is made during the configuration, everything must be corrected on each piece of affected hardware. In this paper, I will be exploring the effect of using a software-defined network controller and a quality of service to handle certain flows of traffic in a network. The main tool used is the OpenFlow defined queue. Queues and flow rules will allow a switch to control individual flows and the network resources that each flow consumes. Factors that will be explored are the bandwidth usage of a flow and the time taken by the network to implement new flow rules. While bandwidth usage is taken into account in traditional networks, changing a quality of service is a new dynamic

Planning calendar for beef cattle herd health

The Oklahoma Cooperative Extension Service periodically issues revisions to its publications. The most current edition is made available. For access to an earlier edition, if available for this title, please contact the Oklahoma State University Library Archives by email at [email protected] or by phone at 405-744-6311