131 research outputs found
Geometry-induced asymmetric diffusion
Past work has shown that ions can pass through a membrane more readily in one
direction than the other. We demonstrate here in a model and an experiment that
for a mixture of small and large particles such asymmetric diffusion can arise
solely from an asymmetry in the geometry of the pores of the membrane. Our
deterministic simulation considers a two-dimensional gas of elastic disks of
two sizes diffusing through a membrane, and our laboratory experiment examines
the diffusion of glass beads of two sizes through a metal membrane. In both
experiment and simulation, the membrane is permeable only to the smaller
particles, and the asymmetric pores lead to an asymmetry in the diffusion rates
of these particles. The presence of even a small percentage of large particles
can clog a membrane, preventing passage of the small particles in one direction
while permitting free flow of the small particles in the other direction. The
purely geometric kinetic constraints may play a role in common biological
contexts such as membrane ion channels.Comment: published with minuscule change
Cross-Validation of a Glucose-Insulin-Glucagon Pharmacodynamics Model for Simulation using Data from Patients with Type 1 Diabetes
Background: Currently, no consensus exists on a model describing endogenous glucose production (EGP) as a function of glucagon concentrations. Reliable simulations to determine the glucagon dose preventing or treating hypoglycemia or to tune a dual-hormone artificial pancreas control algorithm need a validated glucoregulatory model including the effect of glucagon. Methods: Eight type 1 diabetes (T1D) patients each received a subcutaneous (SC) bolus of insulin on four study days to induce mild hypoglycemia followed by a SC bolus of saline or 100, 200, or 300 µg of glucagon. Blood samples were analyzed for concentrations of glucagon, insulin, and glucose. We fitted pharmacokinetic (PK) models to insulin and glucagon data using maximum likelihood and maximum a posteriori estimation methods. Similarly, we fitted a pharmacodynamic (PD) model to glucose data. The PD model included multiplicative effects of insulin and glucagon on EGP. Bias and precision of PD model test fits were assessed by mean predictive error (MPE) and mean absolute predictive error (MAPE). Results: Assuming constant variables in a subject across nonoutlier visits and using thresholds of ±15% MPE and 20% MAPE, we accepted at least one and at most three PD model test fits in each of the seven subjects. Thus, we successfully validated the PD model by leave-one-out cross-validation in seven out of eight T1D patients. Conclusions: The PD model accurately simulates glucose excursions based on plasma insulin and glucagon concentrations. The reported PK/PD model including equations and fitted parameters allows for in silico experiments that may help improve diabetes treatment involving glucagon for prevention of hypoglycemia. </jats:sec
Measuring subdiffusion parameters
We propose a method to extract from experimental data the subdiffusion
parameter and subdiffusion coefficient which are defined by
means of the relation where
denotes a mean square displacement of a random walker starting from
at the initial time . The method exploits a membrane system where a
substance of interest is transported in a solvent from one vessel to another
across a thin membrane which plays here only an auxiliary role. Using such a
system, we experimentally study a diffusion of glucose and sucrose in a gel
solvent. We find a fully analytic solution of the fractional subdiffusion
equation with the initial and boundary conditions representing the system under
study. Confronting the experimental data with the derived formulas, we show a
subdiffusive character of the sugar transport in gel solvent. We precisely
determine the parameter , which is smaller than 1, and the subdiffusion
coefficient .Comment: 17 pages, 9 figures, revised, to appear in Phys. Rev.
Simulating clinical studies of the glucoregulatory system: <i>in vivo</i> meets <i>in silico</i>
Validation of a Simulation Model Describing the Glucose-Insulin-Glucagon Pharmacodynamics in Patients with Type 1 Diabetes
Currently, no consensus exists on a model describing endogenous glucose production (EGP) as a function of glucagon concentrations. Reliable simulations to determinethe glucagon dose preventing or treating hypoglycemia or to tune a dual-hormone artificial pancreas control algorithm need a validated glucoregulatory model including the effect of glucagon
Gastric Emptying Time and Volume of the Small Intestine as Objective Markers in Patients With Symptoms of Diabetic Enteropathy
BACKGROUND/AIMS: Patients with diabetes mellitus (DM) often suffer from gastrointestinal (GI) symptoms, but these correlate poorly to established objective GI motility measures. Our aim is to perform a detailed evaluation of potential measures of gastric and small intestinal motility in patients with DM type 1 and severe GI symptoms. METHODS: Twenty patients with DM and 20 healthy controls (HCs) were included. GI motility was examined with a 3-dimensional-Transit capsule, while organ volumes were determined by CT scans. RESULTS: Patients with DM and HCs did not differ with regard to median gastric contraction frequency (DM 3.0 contractions/minute [interquartile range {IQR}, 2.9-3.0]; HCs 2.9 [IQR, 2.8-3.1]; P = 0.725), amplitude of gastric contractions (DM 9 mm [IQR, 8-11]; HCs 11 mm (IQR, 9-12); P = 0.151) or fasting volume of the stomach wall (DM 149 cm3 [IQR, 112-187]; HCs 132 cm3 [IQR, 107-154]; P = 0.121). Median gastric emptying time was prolonged in patients (DM 3.3 hours [IQR, 2.6-4.6]; HCs 2.4 hours [IQR, 1.8-2.7]; P = 0.002). No difference was found in small intestinal transit time (DM 5 hours [IQR, 3.7-5.6]; HCs 4.8 hours [IQR, 3.9-6.0]; P = 0.883). However, patients with DM had significantly larger volume of the small intestinal wall (DM 623 cm3 [IQR, 487-766]; HCs 478 cm3 [IQR, 393-589]; P = 0.003). Among patients, 13 (68%) had small intestinal wall volume and 9 (50%) had gastric emptying time above the upper 95% percentile of HCs. CONCLUSION: In our study, gastric emptying time and volume of the small intestinal wall appeared to be the best objective measures in patients with DM type 1 and symptoms and gastroenteropathy
Two cations, two mechanisms : interactions of sodium and calcium with zwitterionic lipid membranes
Adsorption of metal cations onto a cellular membrane changes its properties, such as interactions with charged moieties or the propensity for membrane fusion. It is, however, unclear whether cells can regulate ion adsorption and the related functions via locally adjusting their membrane composition. We employed fluorescence techniques and computer simulations to determine how the presence of cholesterol-a key molecule inducing membrane heterogeneity-affects the adsorption of sodium and calcium onto zwitterionic phosphatidylcholine bilayers. We found that the transient adsorption of sodium is dependent on the number of phosphatidylcholine head groups, while the strong surface binding of calcium is determined by the available surface area of the membrane. Cholesterol thus does not affect sodium adsorption and only plays an indirect role in modulating the adsorption of calcium by increasing the total surface area of the membrane. These observations also indicate how lateral lipid heterogeneity can regulate various ion-induced processes including adsorption of peripheral proteins, nanoparticles, and other molecules onto membranes.Peer reviewe
Variational Methods for Biomolecular Modeling
Structure, function and dynamics of many biomolecular systems can be
characterized by the energetic variational principle and the corresponding
systems of partial differential equations (PDEs). This principle allows us to
focus on the identification of essential energetic components, the optimal
parametrization of energies, and the efficient computational implementation of
energy variation or minimization. Given the fact that complex biomolecular
systems are structurally non-uniform and their interactions occur through
contact interfaces, their free energies are associated with various interfaces
as well, such as solute-solvent interface, molecular binding interface, lipid
domain interface, and membrane surfaces. This fact motivates the inclusion of
interface geometry, particular its curvatures, to the parametrization of free
energies. Applications of such interface geometry based energetic variational
principles are illustrated through three concrete topics: the multiscale
modeling of biomolecular electrostatics and solvation that includes the
curvature energy of the molecular surface, the formation of microdomains on
lipid membrane due to the geometric and molecular mechanics at the lipid
interface, and the mean curvature driven protein localization on membrane
surfaces. By further implicitly representing the interface using a phase field
function over the entire domain, one can simulate the dynamics of the interface
and the corresponding energy variation by evolving the phase field function,
achieving significant reduction of the number of degrees of freedom and
computational complexity. Strategies for improving the efficiency of
computational implementations and for extending applications to coarse-graining
or multiscale molecular simulations are outlined.Comment: 36 page
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