Evidence that process simulations reduce anxiety in patients receiving dental treatment: randomized exploratory trial

Process simulations – mental simulations that ask people to imagine the process of completing a task – have been shown to decrease anxiety in students facing hypothetical or psychological threats in the short term. The aim of the present study was to see whether process simulations could reduce anxiety in a sample of the general population attending a dental practice, and whether these effects could be sustained throughout treatment. Participants (N = 75) were randomized to an experimental condition where they were asked to simulate mentally the process of seeing the dentist, or to a control condition where they were asked to simulate mentally the outcome of seeing the dentist. Findings showed that participants in the experimental condition were significantly less anxious both before and after their consultations. Self-efficacy and self-esteem remained unchanged. This study suggests that process simulation is one active ingredient in anxiety treatment programs and further research is required to enhance its effects

Tides of change : identifying the neural correlates of motor sequence learning

The present study combines behavioural and neuroimaging techniques to investigate the learning of a motor sequence task within- and across-day. The goals of this study were to identify behavioural changes during learning, determine the patterns of activity associated with learning, and investigate the relationships between behaviour and brain activity over the course of learning. Participants were trained on a relatively complex motor sequencing task and a simple baseline task. They performed these tasks in an fMRI scanner while behavioural and functional data were collected. Behavioural performance increased within, but not across, day. The initial learning network included activity in CB cortex, posterior BG, PMC, PL, and preSMA/SMA. Within-day changes included increased activity in anterior BG, CB nucleus, and STS; with decreased activity in M1/S1, SMA, and planum temporale. Across-day increases were found in the left anterior BG, no decreases were found across-day. These results may be interpreted as a shift in activity from the visual to the spatial corticostriatal loop, and are discussed within the context of two current theories of motor sequence learning

"Why don't they read a good book instead?": Librarians and Comic Books, 1949-1955

This paper surveys the attitudes of librarians towards efforts to restrict and censor comic books in the period from 1949-1955. This period includes the years leading up to and just beyond the creation of the Comics Code in 1954, an event that censored comic books determined to be obscene. The analysis finds that librarians in the late 1940s and early 1950s were divided over exactly how to approach the issue of comic books and children. Part of this ambivalence stemmed from the inconclusive research regarding relationship between violent comic books and juvenile delinquency. Moreover, the anti-censorship position that is fundamental to librarianship further complicated the actions of librarians. The study concludes that librarians were no better or worse than other cultural ambassadors of the era in their reaction to comic books

Verifying likelihoods for low template DNA profiles using multiple replicates

AbstractTo date there is no generally accepted method to test the validity of algorithms used to compute likelihood ratios (LR) evaluating forensic DNA profiles from low-template and/or degraded samples. An upper bound on the LR is provided by the inverse of the match probability, which is the usual measure of weight of evidence for standard DNA profiles not subject to the stochastic effects that are the hallmark of low-template profiles. However, even for low-template profiles the LR in favour of a true prosecution hypothesis should approach this bound as the number of profiling replicates increases, provided that the queried contributor is the major contributor. Moreover, for sufficiently many replicates the standard LR for mixtures is often surpassed by the low-template LR. It follows that multiple LTDNA replicates can provide stronger evidence for a contributor to a mixture than a standard analysis of a good-quality profile. Here, we examine the performance of the likeLTD software for up to eight replicate profiling runs. We consider simulated and laboratory-generated replicates as well as resampling replicates from a real crime case. We show that LRs generated by likeLTD usually do exceed the mixture LR given sufficient replicates, are bounded above by the inverse match probability and do approach this bound closely when this is expected. We also show good performance of likeLTD even when a large majority of alleles are designated as uncertain, and suggest that there can be advantages to using different profiling sensitivities for different replicates. Overall, our results support both the validity of the underlying mathematical model and its correct implementation in the likeLTD software

Water Movement through the Unsaturated Zone of the High Plains Aquifer in the Central Platte Natural Resources District, Nebraska, 2008-12

Uncertainty about the effects of land use and climate on water movement in the unsaturated zone and on groundwater recharge rates can lead to uncertainty in water budgets used for groundwater-flow models. To better understand these effects, a cooperative study between the United States Geological Survey (USGS) and the Central Platte Natural Resources District (CP NRD) was initiated in 2007 to determine field-based estimates of recharge rates in selected land-use areas of the CP NRD in Nebraska. Measured total water potential and unsaturated-zone profiles of tritium, chloride, nitrate as nitrogen, and bromide, along with groundwater-age dates, were used to evaluate water movement in the unsaturated zone and groundwater recharge rates in the central Platte River study area. Eight study sites represented an east-west precipitation contrast across the study area—four beneath groundwater-irrigated cropland (sites 2, 5, and 6 were irrigated corn and site 7 was irrigated alfalfa/corn rotation), three beneath rangeland (sites 1, 4, and 8), and one beneath nonirrigated cropland, or dryland (site 3).Measurements of transient vertical gradients in total water potential indicated that periodic wetting fronts reached greater mean maximum depths beneath the irrigated sites than beneath the rangeland sites, in part, because of the presence of greater and constant antecedent moisture. Beneath the rangeland sites, greater temporal variation in antecedent moisture and total water potential existed and was, in part, likely a result of local precipitation and evapotranspiration. Moreover, greater variability was noticed in the total water potential profiles beneath the western sites than the corresponding eastern sites, which was attributed to less mean annual precipitation in the west.The depth of the peak post-bomb tritium concentration or the interface between the pre-bomb/post-bomb tritium, along with a tritium mass balance, within sampled soil profiles were used to estimate water fluxes in the unsaturated zone at three of the eight study sites: site 2 (irrigated), site 3 (dryland), and site 8 (rangeland). Estimates for recharge were about 68 millimeters per year [(mm/yr), post-bomb peak], 133 to 159 mm/yr (tritium interface), and 137 mm/yr (mass balance) at site 2 (irrigated); about 63 mm/yr (tritium interface) and 12 mm/yr (mass balance) at site 3 (dryland); and about 53 mm/yr (tritium interface) and 10 mm/yr (mass balance) at site 8 (rangeland). Recharge values from the mass balance at site 2 were more than an order of magnitude greater than recharge values at site 3, suggesting irrigation is an important control on water movement through the unsaturated zone. For the remaining five sites, the post-bomb tritium had flushed through the system and recharge was considered modern (within 10 years of sampling).The chloride mass-balance method was used to determine water fluxes below the root zone (less than 2 meters below land surface) at the rangeland sites: sites 1, 4, and 8. At these rangeland sites, water fluxes ranged from 1.8 to 96 mm/yr at site 1, 1.1 to 9.6 mm/yr at site 4, and 1.1 to 68 mm/yr at site 8, with mean rates of 21, 4.3, and 13 mm/yr, respectively. Site 1 had a greater mean water flux, which was consistent with the greater precipitation in the east than at site 8 in the west. Chloride mass balance was not calculated at the irrigated and dryland sites because of uncertainty about additional sources of chloride.Concentrations of nitrate as nitrogen in pore water in the unsaturated zone were larger beneath the irrigated and dryland (agricultural) sites compared with the rangeland sites. The larger concentrations at the agricultural sites are consistent with the application of nitrogen fertilizer at the agricultural sites and no substantial accumulation at the rangeland sites. The shape of the nitrate as nitrogen and chloride concentration profiles at site 1 (rangeland) indicate a reasonably larger and more consistent water flux in the UZ than beneath the other two rangeland sites (sites 4 or 8). Excluding site 7, the general shape of the nitrate as nitrogen profiles was similar beneath the agricultural sites and supports the estimates of water movement and recharge rates determined from the tritium and chloride methods.Movement of bromide through the unsaturated zone indicated greater water fluxes are found beneath irrigated lands than beneath rangeland. Bromide profiles in the unsaturated zone, determined from center of mass and peak displacement methods, document water fluxes ranged from 58 to 394 mm/yr beneath irrigated sites and 9 to 201 mm/yr beneath rangeland sites. Water-flux estimates from the potassium bromide tests at most sites did not represent overall recharge rates because the bromide remained primarily in the root zone.Apparent groundwater age was used to determine the groundwater residence time at the eight sites and to estimate recharge rates. Groundwater ages in the study area ranged from old water (defined here as groundwater that was recharged more than 50 years ago) to modern (defined here as groundwater that has recharged within the past 10 years). Groundwater ages indicated that the shallow monitoring wells generally had younger residence times, whereas the deeper monitoring wells generally had the older residence times. Groundwater dates from the shallowest monitoring wells were used to determine recharge rates at the water table. These rates generally were similar to recharge rates determined from tritium and chloride mass-balance methods. Groundwater recharge rates generally increased with well depth, and the deeper monitoring wells likely do not represent local recharge conditions but recharge from a regional flow system that receives recharge from distant sources.Overall, these data generally indicate that water movement within the unsaturated zone primarily is affected by spatial contrasts in mean annual precipitation and by the land use or land cover. The eight unsaturated-zone sites each generated unique, valuable datasets that likely will improve the understanding of water movement and recharge rates in the central Platte River valley

A Longitudinal Mixed Logit Model for Estimation of Push and Pull Effects in Residential Location Choice

We develop a random effects discrete choice model for the analysis of households' choice of neighbourhood over time. The model is parameterised in a way that exploits longitudinal data to separate the influence of neighbourhood characteristics on the decision to move out of the current area (\push" effects) and on the choice of one destination over another (\pull" efdfects). Random effects are included to allow for unobserved heterogeneity between households in their propensity to move, and in the importance placed on area characteristics. The model also includes area-level random effects. The combination of a large choice set, large sample size and repeated observations mean that existing estimation approaches are often infeasible. We therefore propose an effcient MCMC algorithm for the analysis of large-scale datasets. The model is applied in an analysis of residential choice in England using data from the British Household Panel Survey linked to neighbourhood-level census data. We consider how effects of area deprivation and distance from the current area depend on household characteristics and life course transitions in the previous year. We find substantial differences between households in the effects of deprivation on out-mobility and selection of destination, with evidence of severely constrained choices among less-advantaged households

\u3ci\u3e Phenytoin reduces 5-ala mediated fluorescence in glioblastoma cells \u3c/i\u3e

Glioblastoma multiforme (GBM) is a devastating form of cancer, and essentially all GBM tumors recur causing fatality. A new surgical technique, fluorescence-guided resection of GBM using 5-aminolevulinic acid (5-ala), improves the extent of resection and positively impacts the length and quality of patient survival. The fluorescence achieved in neoplastic tissue depends directly on the accumulation of porphyrins derived from the metabolism of the 5-ala prodrug within the cancer cell. However, 5-ala induced fluorescence has been reported to be inconsistent. In an effort to determine the cause of the inconsistent fluorescence, the authors investigated the effect of medications commonly prescribed to brain tumor patients on 5-ala induced fluorescence. A model was developed to quantify intracellular porphyrin accumulation using a U87MG GBM cell line constitutively expressing yellow fluorescent protein (YFP-U87). 5-ala mediated fluorescence within the cells was standardized to cell number via the fluorescence emission spectra ratio of porphyrin (405 nm) to YFP (525 nm). 5-ala induced accumulation of porphyrins was measured after treating YFP-U87 cells with phenytoin, dexamethasone, or desipramine for 3 days. After a 6 hour incubation with 5-ala, no significant difference in porphyrin accumulation was observed in cells treated with dexamethasone or desipramine. Phenytoin, however, significantly reduced the accumulation of fluorescent porphyrins within the YFP-U87 cell line by nearly 30% compared to the control. To optimize fluorescence during surgery and improve patient survival these results suggest that further investigations are warranted to determine the effects of commonly administered medications on 5-ala fluorescence-guided resection of GBM

Reliability of ultrasound to measure morphology of the toe flexor muscles

Measuring the strength of individual foot muscles is very challenging; however, measuring muscle morphology has been shown to be associated with strength [1]. A reliable method of assessing foot muscle atrophy and hypertrophy would therefore be beneficial to researchers and clinicians. Real-time ultrasound (US) is a non-invasive, objective and inexpensive method of assessing muscle morphology and has been employed widely to quantify cross-sectional area (CSA) and linear dimensions of larger muscles (e.g. quadriceps, triceps surae). Few studies, however, have determined its ability to measure the small muscles of the foot and ankle. This study aimed to determine whether US is a reliable tool to measure the morphology of the toe flexor muscles

Anodal Transcranial Direct Current Stimulation Does Not Facilitate Dynamic Balance Task Learning in Healthy Old Adults

Older adults frequently experience a decrease in balance control that leads to increased numbers of falls, injuries and hospitalization. Therefore, evaluating older adults’ ability to maintain balance and examining new approaches to counteract age-related decline in balance control is of great importance for fall prevention and healthy aging. Non-invasive brain stimulation techniques such as transcranial direct current stimulation (tDCS) have been shown to beneficially influence motor behavior and motor learning. In the present study, we investigated the influence of tDCS applied over the leg area of the primary motor cortex (M1) on balance task learning of healthy elderly in a dynamic balance task (DBT). In total, 30 older adults were enrolled in a cross-sectional, randomized design including two consecutive DBT training sessions. Only during the first DBT session, either 20 min of anodal tDCS (a-tDCS) or sham tDCS (s-tDCS) were applied and learning improvement was compared between the two groups. Our data showed that both groups successfully learned to perform the DBT on both training sessions. Interestingly, between- group analyses revealed no difference between the a-tDCS and the s-tDCS group regarding their level of task learning. These results indicate that the concurrent application of tDCS over M1 leg area did not elicit DBT learning enhancement in our study cohort. However, a regression analysis revealed that DBT performance can be predicted by the kinematic profile of the movement, a finding that may provide new insights for individualized approaches of treating balance and gait disorders

Rapid Quantification of White Matter Disconnection in the Human Brain

With an estimated five million new stroke survivors every year and a rapidly aging population suffering from hyperintensities and diseases of presumed vascular origin that affect white matter and contribute to cognitive decline, it is critical that we understand the impact of white matter damage on brain structure and behavior. Current techniques for assessing the impact of lesions consider only location, type, and extent, while ignoring how the affected region was connected to the rest of the brain. Regional brain function is a product of both local structure and its connectivity. Therefore, obtaining a map of white matter disconnection is a crucial step that could help us predict the behavioral deficits that patients exhibit. In the present work, we introduce a new practical method for computing lesion-based white matter disconnection maps that require only moderate computational resources. We achieve this by creating diffusion tractography models of the brains of healthy adults and assessing the connectivity between small regions. We then interrupt these connectivity models by projecting patients' lesions into them to compute predicted white matter disconnection. A quantified disconnection map can be computed for an individual patient in approximately 35 seconds using a single core CPU-based computation. In comparison, a similar quantification performed with other tools provided by MRtrix3 takes 5.47 minutes.Comment: 2020 42nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC