Electrochemical separation of 90-yttrium in the electrochemical 90Sr/90Y generator and its use for radiolabelling of DOTA-conjugated somatostatin analog [DOTA0, Tyr3] octreotate

Radiopharmaceuticals based on 90Y are widely used in the treatment of malignant deseases. In order to meet the requirements for their future application, a 90Sr/90Y generator was developed and 90Y eluted from this locally produced generator was used for the radiolabelling of the DOTA-conjugated somatostatin analog [DOTA0,Tyr3] octreotate and the preparation of [90Y-DOTA0,Tyr3] octreotate (90Y-DOTATATE) for peptide receptore radionuclide therapy. 90Sr/90Y generator was based on the electrochemical separation of 90Y from 90Sr in a two-cycle electrolysis procedure. Three electrode cells were used to perform both electrolyses. In both cycles, working electrodes were kept on constant potential. The pH of the solution was adjusted to 2.7 of the value before the electrolyses. The radionuclidic purity of the 90Y solution was analysed by ITLC and extraction paper chromatography. The labelling of peptide (100 mg DOTATATE) with 90YCl3 was performed at 95°C for 30 minutes. Radiochemical purity was determined by HPLC and chromatographic separation, using a solid SepPak C-18 column. Results obtained confirmed the efficiency of our electrochemical separation technique and quality control methods for 90Y. The achieved efficiency of the 90Sr/90Y generator above 96% of the theoretical value represents a good basis for the further development of this generator. The labelling of the DOTATATE with 90Y exhibited a high efficiency, too: there was less than 1% of 90Y3+in the 90Y-DOTATATE

Cuticular chemoprofile of the fruit fly drosophila subobscura (diptera, drosophilidae)

In insects, cuticular hydrocarbon (CHC) profile is involved in many important biological functions and may vary in different conditions. Among fruit fly species, Drosophila subobscura is one of the most frequently used in genetic, ecological and evolutionary research, because of its rich chromosomal polymorphism, specific behavioral repertoires and habitat preferences. In this work, we identified and quantified cuticular chemoprofile of D. subobscura. Using gas chromatography (GC) and gas chromatography coupled with mass spectrometry (GC-MS), 25 chemical compounds were found in males and 23 compounds were found in females. Further, ANOVA confirmed significant sexual dimorphism in cuticular chemoprofile amounts. Knowledge of cuticular chemistry could contribute to further research in D. subobscura, starting from behavioral, up to ecological, since this species is recognized as an important model system for the study and monitoring of global climate changes

Efficient separation of Tc-99 from aqueous solution using pinecone activated carbon

Sorption of radioactive 99Tc-pertechnetate onto activated carbon prepared from pinecones was investigated. The parameters of contact time and Tc-99 concentration were varied. It was established that the sorption half-life, t1/2, was very short ~ 2.2 min, thus making this sorbent an excellent candidate for immediate pollution remediation, which is very important in the case of nuclear accidents. The kinetic data were found to follow closely the pseudosecond- order (PSO) kinetic model. Boyd model established the sorption dependence only on film diffusion. The partitioning/distribution coefficient, Kd, attained was 2729 cm3 g-1. The equilibration time was 3 h at room temperature (T=298 K)

Cuticular chemoprofile of the fruit fly Drosophila Subobscura (Diptera, drosophilidae)

In insects, cuticular hydrocarbon (CHC) profile is involved in many important biological functions and may vary in different conditions. Among fruit fly species, Drosophila subobscura is one of the most frequently used in genetic, ecological and evolutionary research, because of its rich chromosomal polymorphism, specific behavioral repertoires and habitat preferences. In this work, we identified and quantified cuticular chemoprofile of D. subobscura. Using gas chromatography (GC) and gas chromatography coupled with mass spectrometry (GC-MS), 25 chemical compounds were found in males and 23 compounds were found in females. Further, ANOVA confirmed significant sexual dimorphism in cuticular chemoprofile amounts. Knowledge of cuticular chemistry could contribute to further research in D. subobscura, starting from behavioral, up to ecological, since this species is recognized as an important model system for the study and monitoring of global climate changes. © 2018, Pakistan Agricultural Scientists Forum. All rights reserved

Cuticular chemoprofile of the fruit fly drosophila subobscura (diptera, drosophilidae)

In insects, cuticular hydrocarbon (CHC) profile is involved in many important biological functions and may vary in different conditions. Among fruit fly species, Drosophila subobscura is one of the most frequently used in genetic, ecological and evolutionary research, because of its rich chromosomal polymorphism, specific behavioral repertoires and habitat preferences. In this work, we identified and quantified cuticular chemoprofile of D. subobscura. Using gas chromatography (GC) and gas chromatography coupled with mass spectrometry (GC-MS), 25 chemical compounds were found in males and 23 compounds were found in females. Further, ANOVA confirmed significant sexual dimorphism in cuticular chemoprofile amounts. Knowledge of cuticular chemistry could contribute to further research in D. subobscura, starting from behavioral, up to ecological, since this species is recognized as an important model system for the study and monitoring of global climate changes

Cuticular chemoprofile of the fruit fly Drosophila Subobscura (Diptera, drosophilidae)

In insects, cuticular hydrocarbon (CHC) profile is involved in many important biological functions and may vary in different conditions. Among fruit fly species, Drosophila subobscura is one of the most frequently used in genetic, ecological and evolutionary research, because of its rich chromosomal polymorphism, specific behavioral repertoires and habitat preferences. In this work, we identified and quantified cuticular chemoprofile of D. subobscura. Using gas chromatography (GC) and gas chromatography coupled with mass spectrometry (GC-MS), 25 chemical compounds were found in males and 23 compounds were found in females. Further, ANOVA confirmed significant sexual dimorphism in cuticular chemoprofile amounts. Knowledge of cuticular chemistry could contribute to further research in D. subobscura, starting from behavioral, up to ecological, since this species is recognized as an important model system for the study and monitoring of global climate changes. © 2018, Pakistan Agricultural Scientists Forum. All rights reserved

Characteristics of Wastewater Streams Within the Bor Copper Mine and Their Influence on Pollution of the Timok River, Serbia

Wastewaters produced by mining and metallurgical treatment of polymetallic sulfide ores have polluted the Timok River in Serbia for more than 100 years. We have characterized seven different wastewater streams within the RTB Bor facilities, investigating their influence on pollution of the Timok River before its confluence with the Danube River. Their relative contribution was calculated by considering the contaminant load of each wastewater stream and non-conservative transport along their flow paths. Three sampling spots in the Timok River, before and after its confluence with the Borska River, were also monitored

Analysis of forest vegetation in Koviljski Rit: Comparison of habitats with varying degrees of anthropogenic influence

Koviljski Rit is located along the left bank of the Danube River, between the villages of Kovilj and Gardinovci. It is a rare example of pristine nature, where the original features of a swamp biotope are preserved. Because of its exceptional natural value, Koviljski Rit, along with the swamp in Petrovaradin, has been under the protection of the Republic of Serbia since 1998, when it was proclaimed a Special Nature Reserve of the first category. Natural vegetation found in Koviljski Rit includes woodlands composed of willow and poplar. The majority of the reserve consists of forest plantations (55.6%), which include habitats influenced by human activities. Based on DCA (Detrended correspondence analysis) of forest vegetation in Koviljski Rit, there is a high degree of similarity between natural populations of white willow and stands of anthropogenic origin. However, in contrast, the same analysis indicates clear differences between natural and artificial poplar stands. Moreover, depending on whether they were planted in temporary or permanent flood plain habitats, significant differences are evident in the floristic structures of Euro-American poplar forests. Ecological conditions in habitats of white and Euro-American poplar (Populus x euroamericana/Dode/ Guinier) communities were found to be more similar with respect to illumination and soil humidity. However, more importantly, the anthropogenic forests recorded the lowest diversity as a result of regular thinning and measures implemented through forest management. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III 43002: Biosensing technologies and global system for continuous research and integrated management of ecosystems and Grant, no. 31,041th: Forest plantations as a way to increase reforestation in Serbia

The attitudes of teachers towards inclusive education in Vojvodina

The aim of this study was to examine the attitudes of preschool, primary, secondary and high school teachers towards inclusive education of children with special educational needs. In addition, the study established the correlation between these attitudes and gender, education level, teaching experience, formal training in the special education field, and the duration and quality of work experience with children with special education needs. The sample comprised 322 teachers from the Serbian province of Vojvodina. The My Thinking about Inclusion Scale (Stoiber, K. C., M. Gettinger, and D. Goetz. 1998. "Exploring Factors Influencing Parents' and Early Childhood Practitioners Beliefs about Inclusion." Early Childhood Research Quarterly 13 (1): 107-131) was used. The results show that, in general, the participants held neutral attitudes towards inclusive education and more positive expectations regarding the outcomes of inclusion. This study also emphasised teaching performance in an inclusive class as a subject of great concern. The high school and preschool teachers as well as the teachers with previous positive experience with working in an inclusive environment reported more positive attitudes towards inclusive education than those from primary and secondary schools and those with negative experiences with the implementation of inclusive practices