321 research outputs found

    Activity of antioxidative defense enzymes in the blood of patients with liver echinococcosis

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    Background/Aim. Chronic echinococcocal disease is the parasite human disease caused by the penetration of larval (asexual) stages of the canine tapeworm (Echinococcus granulosus) in the liver of humans. After the penetration of the parasite, the host organism react by activating complement- depending immune response. The aim of this study was to elucidate the influence of larval form of Echinococcus granulosus in the liver on the activity of antioxidative defense enzymes in the blood of patients before and after the surgical intervention. Methods. We investigated the activity of antioxidative defense enzymes: copper/zinc containing superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in the blood of patients before and after the surgical intervention in respect to the controls, clinically healthy persons. Results. Our results showed that the activity of the GSH-Px was significantly decreased in the plasma of the patients with echinocococal disease before the surgery in respect to the controls. The activity of GST was significantly higher in the blood of the patients after the surgery in comparison to the controls. Conclusion. Chronic liver echinoccocal disease caused significant changes of some antioxidative defense enzymes, first of all Se-dependent enzyme GSH-Px, which could be a suitabile biomarker in the biochemical evaluation of the disease. This work represents a first comprehensive study of the activity of antioxidative defense enzymes in cronic liver echinococcocosis in the patients before and after the surgical intervention in respect to the clinically healthy persons

    The impact of resistance training program on the muscle strength and bone density in adolescent athletes

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    © 2018 Saša Bubanj et al., published by Sciendo 2018. Strength training and other modes of physical activity may be beneficial in osteoporosis prevention by maximizing bone mineral accrual in childhood and adolescence. This study focuses on the impact of the nine-month long program of resistant exercises with different level of external loads (low, middle and high) on the lower limbs explosive strength and bone tissue density in athletes adolescents aged 17 to 18 years. Sixty healthy, male athletes and non-athletes, divided into experimental (ES, sprinters, N = 45) and control sub-sample (CS, non-athletes, N = 15), were included in study. ES examinees (EG1, EG2 and EG3) were subjected to the program of resistance exercises with low level (60% of the One Repetition Maximum-1RM), middle level (70% 1RM), and high level (85% 1RM) of external loads, respectively. Bone Density values were determined by the use of a clinical sonometer.,Sahara (Hologic, Inc., MA 02154, USA). Explosive strength values of hip extensors and flexors, knee extensors and flexors, and ankle plantar and dorsiflexors were determined by the use of accelerometer.,Myotest (Sion, Switzerland) and the means of Counter Movement Jump without arms swing (CMJ) and half squat. ANOVA method for repeated measures and ANCOVA method were used to determine significant differences and resistance program effects on the lower limbs explosive strength and bone tissue density. Resistance exercise does impact the explosive strength and bone parameters in a way to increase half squat 1RM values, but decreases CMJ values, and increases speed of sound (SOS), broadband ultrasound attenuation (BUA) and bone mineral density (BMD) values in athletes-adolescents, aged 17-18 years

    Antimicrobial and genotoxic activity of novel ruthenium(III) complex with n-phenyl-5-nitrosalicylideneimine

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    In this study, novel hexa coordinated ruthenium(III) complex of the type Na[RuCl2L2)] (where L = monobasic bidentate Schiff base derived from the condensation of 5-nitrosalicyladehyde with aniline) has been synthesized and characterized by electrospray ionization time-of-flight mass spectrometry, infrared spectroscopy and ultraviolet/visible spectrophotometry. Schiff base N-phenyl-5-nitrosalicylideneimine is coordinated to the ruthenium via imine nitrogen and phenolic oxygen. Mass spectra showed molecular ion (M-) at m/z 653.9641 which corresponds to [C26H18Cl2N4O6Ru]-. The in vitro antimicrobial properties of the Schiff base and the complex were tested by micro-dilution technique and agar plate assay for determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The compounds showed a higher antibacterial activity against tested Gram-positive bacteria (Staphylococcus aureus ATCC 33591 and ATCC 29213), whereas against the Gram-negative bacteria (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603) were ineffective. The genotoxic effects of Ru(III) complex were investigated using the Cytokinesis Block Micronucleus (CBMN) assay in human lymphocytes cultures. The cell culture treated with the complex at a concentration of 3.7 µg/mL exhibit the most prominent effect of decreasing the frequency of micronucleus for 44%, while at the concentrations of 1.5 and 7.4 µg/mL effect is slightly lower (40%), compared to the control cell culture

    Radiation sensitive MOSFETs irradiated with various positive gate biases

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    The RADiation sensitive metal-oxide-semiconductor field-effect-transistors (RADFETs) were irradiated with gamma rays up to absorbed dose of 110 Gy(H2O). The results of threshold voltage, VT, during irradiation with various positive gate biases showed the increase in VT with gate bias. The threshold voltage shift, ΔVT, during irradiation was fitted very well. The contributions of both the fixed traps (FTs) and switching traps (STs) during radiation on ΔVT were analyzed. The results show the significantly higher contribution of FTs than STs. A function that describes the dependence of threshold voltage shift and its components on gate bias was proposed, which fitted the experimental values very well. The annealing at the room temperature without gate bias of irradiated RADFETs was investigated. The recovery of threshold voltage, known as fading, slightly increase with the gate bias applied during radiation. The ΔVT shows the same changes as the threshold voltage component due to fixed states, ΔVft, while there is no change in the threshold voltage component due to switching traps, ΔVst

    Fatty acid in colorectal cancer in adult and aged patients of both sexes

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    Purpose: Colorectal cancer represents the second most common type of cancer in Serbia. Alteration of lipid metabolism begins early, and can represent a central hallmark in cancer evolution. Fatty acids have various important functions as building components of cell membranes, as signaling molecules in immune responses and also manage the general cancer signaling network. The purpose of this study was to investigate the difference of various fatty acids content between colorectal cancer and adjacent healthy intestinal tissue in adult and aged patients of both sexes. Methods: 52 subjects participated in this study. Healthy colon mucosa and tumor tissue samples were obtained from patients previously diagnosed with colorectal carcinoma. Simplified method of Berstad et al was used for direct transesterification of total lipids in tumor and healthy mucosa tissue samples and separations of the methyl esters was carried out using a gas chromatograph equipped with a split/ splitless injector and a flame ionization detector. Results: 18 0, 18 1 n7, 20 3, 20 4, 20 5, 22 4, 22 5 22 6, SFA, PUFA, n6, n3 and AA/EPA were significantly higher in tumor tissue. On the other hand, 18 1 n9, 18 2, 18 3 n3, MUFA, n6/ n3 were significantly higher in healthy tissue. Conclusions: Saturation index (SI) could be a valuable tool to delineate robust immune response and worse prognosis in patients with colorectal cancer. Our study demonstrated significant differences in fatty acid profiles between tumor tissue and healthy mucosa. Parameters, such as gender, age, stage and mucinous component didn't influence altered fatty acid content

    Algae as a source of bioactive compounds to prevent the development of type 2 diabetes mellitus

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    Type 2 diabetes mellitus is a complicated metabolic disorder characterized by hyperglycemia and glucose intolerance. It is considered a new pandemic and its control involves numerous challenges. Although many of the measures are based on improving life habits, diet is also of vital importance due to bioactive compounds present in food. In this regard, several raw materials have been investigated whose bioactivities seem to slow the progression of this disease. Within these matrices, there are algae of importance, such as brown algae, showing to have beneficial effects on glycemic control. These pieces of evidence are increasing every day due to the development of cell or animal models, which lead to the conclusion that bioactive compounds may have direct effects on decreasing hyperglycemia, enhancing insulin secretion and preventing the formation of amyloid plaques.Serbian co-authors thank the Ministry of Education, Science, and Technological Development of the Republic of Serbia for the support within the contracts for the realization and financing of scientific research work in 2021 ; financial support from Programa de Cooperación Interreg V-A España—Portugal (POCTEP) 2014–2020; MICINN supporting the Ramón&Cajal grant for M.A. Prieto; to Xunta de Galicia and University of Vigo supporting the pre-doctoral grants for A.G. Pereira, and P. García-Oliveira; to Ibero-American Program on Science and Technology for financial support. This project has received funding from the Bio Based Industries Joint Undertaking (JU); the JU receives support from the European Union’s Horizon 2020 research and innovation program and the Bio Based Industries Consortium.info:eu-repo/semantics/publishedVersio

    The most common health disorders and welfare of dairy cows and calves

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    Three farms of dairy cows (A, B and C) were observed for health disorders of dairy cows and suckling calves. Farm A is farm with tied system of rearing, with 1100 cows, while farms B and C have 400 and 600 cows kept loose with outdoor pens, respectively. Data regarding welfare criteria of dairy cows (health, feeding, housing and behaviour) were collected and analysed through Protocol of Welfare Quality (2009). Health disorders of dairy cows and suckling calves were collected and statistically analysed by chi-square test (X2 test). Welfare of all of three dairy farms were assessed as acceptable, meaning that provided welfare conditions meet the minimum requirements of animals. Principle of provision of good health was rated as acceptable (≥20 points) on farm A, while on farms with loose system (B and C) overall health rated as excellent (≥80 points). Occurrence rate of reproductive, locomotor, skin and claws disorders and digestive and systemic disorders of dairy cows and calves up to 4 months old were very different between three farms (A, B, C) with X2-values of 2901.71, 252.02, 204.08, 1152.31 and 184.23 respectively; α<0.01). According presented data, it is obvious that the majority health problems were observed in tied system of rearing, on farm A, such as reproductive disorders and mastitis, as well as injuries and bad body score and lame cows. The most serious health problems of the calves were diarrhea and bronchopneumonia of different etiology

    Gamma Radiation Absorption Characteristics of Concrete with Components of Different Type Materials

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    Nuclear facilities as nuclear power stations, nuclear research reactors, particle accelerators and linear accelerator in medical institution using concrete in building construction. The different type materials of the aggregate as component of concrete were analyzed to provide radiation protection. The energy deposited the transmission factor and the mass attenuation coefficients in ordinary and barite concretes have been calculated with the photon transport Monte Carlo software. The numerical simulations results show that using barite as an aggregate in the concrete is one of the solutions for gamma ray shielding. Thereat, it is shown non-destructive method for determining the gamma radiation absorption characteristics of concrete.11th Annual Conference of the Materials-Research-Society-of-Serbia (YUCOMAT 2009), Aug 31-Sep 04, 2009, Herceg Novi, Montenegr
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