AN APPROACH TO THE DEVELOPMENT OF THE URBAN DESIGN “KARAOTOK”, HUTOVO BLATO NATURE PARK

Prostorno planiranje je vitalni proces za brzo i uspješno razvijanje projekata vezanih za upravljanje vlažnim staništima, time i projektima vezanim za turizam. Turističke aktivnosti mogu dati obol razvijanju općeg razumijevanja, te podizanju pozornosti javnosti za vlažna staništa i njihove funkcije i ekološke sustave. Pored toga, turizam može biti i izvor prihoda te može pružiti mogućnosti zapošljavanja u ruralnim sredinama. Turisti također imaju utjecaj na vlažna staništa. Mjesta koja su dostupna turistima traže intenzivnije upravljanje negoli ona koja im nisu pristupačna. U cilju osiguranja skladnog odnosa između turističkih aktivnosti i zaštite okoliša prijeko je potrebno pristupiti procesu izrade urbanističkog projekta u sklopu zaštićenog područja na iznimno senzibilan način. U radu je sugeriran pristup izradi Urbanističkog projekta „Karaotok“, u sklopu Parka prirode Hutovo blato.Spatial planning is a vital process for a rapid and successful development of projects related to wetland management, and thereby projects related to tourism. Tourist activities could provide a boost to the development of general understanding of wetlands and raise public awareness of the functions and the ecosystem services wetlands provide. The tourism economy could be a source of income and could provide employment opportunities in rural areas. However, tourists also have an impact on wetlands. Protected areas accessible to tourists require a comprehensive management. In order to ensure a harmonious relationship between tourism activities and environmental protection, when developing an urban design within a protected area, it is crucial to have a sensitive approach. The paper suggests an approach to the development of the Urban Design "Karaotok", within the Hutovo Blato Nature Park

Biodiversity and seasonal distribution of benthic diatom assemblages as an indicator of water quality of small karstic river in Bosnia and Herzegovina

The aims of this paper were to describe seasonal changes in the qualitative and quantitative composition of diatom taxa and the potential application of benthic diatoms for ecological status evaluation. Diatom indices (IPS and TI) were calculated from data from three different locations along a longitudinal profile of the Bunica, a small karstic river in Bosnia and Herzegovina. A total of 147 taxa were recorded in 12 samples. The most common taxa were Meridion circulare (Greville) C.Agardh and Ulnaria ulna (Nitzsch) Compère. Physical and chemical analyses showed low concentrations of nutrients, good oxygenation, typical pH for carbonate bed/origin and generally oligotrophic conditions and high ecological status. All sites had similar physico-chemical conditions and there were only few seasonal differences. Ordination of the diatom data showed that samples showed neither longitudinal nor seasonal patterns. Median value for IPS (16.8) and for TI (7.3) can be possible ‘‘expected’’ values for ecological status assessment for small karstic rivers in the Mediterranean region. We propose the use of the phytobenthos Intercalibration Common Metric (pICM - an index that combines the IPS and TI) as a national metric for countries developing WFD diatom methods at a late stage. One situation is described, and a solution, which is potentially transferable to other locations in Bosnia and Herzegovina and also to other countries facing similar challenges

Odrasli tulari (Insecta: Trichoptera) kao pokazatelji ekološkog statusa rijeke Lištice, Bosna i Hercegovina

A study of caddisfly biodiversity and its application for use as indicator species to assess the ecological integrity of aquatic environments was conducted in the area of the Lištica River, Bosnia and Herzegovina. A portable UV lighttrap was used to collect caddisflies at two sites: the spring of Lištica - Bilo Vrilo and the middle reach of this river in a karstic depression - Mostarsko Blato. From March 2003 to March 2004 a total of 4334 individuals, representing 34 species, were caught. There were signifi cant differences in species composition and abundance between sampling sites. The flight periods are shown for all recorded species and studied in detail for twelve abundant species. The species inventories were used for analysing the longitudinal classification of the sampling sites, composition of functional feeding guilds and the saprobic indices.Istraživana je bioraznolikost tulara rijeke Lištice te primjena njihovih zajednica u procjeni ekološkog statusa vodenih ekosustava. Odrasle jedinke uzorkovane su na dvjema postajama, tj na izvoru rijeke Bilo vrilo i središnjem dijelu toka Lištice, u krškom polju Mostarskom blatu. Materijal je prikupljan godinu dana (ožujak 2003. – ožujak 2004.) metodom lova pomoću UV lampe. Istraživanjima su zabilježene 34 vrste s 4334 uzorkovanih jedinki. Utvrđene su značajne razlike u fauni tulara na dvjema istraživanim postajama, koje su se ogledale u sastavu zajednica i brojnosti uzorkovanih jedinki. U radu je prikazan period aktivnosti svih zabilježenih vrsta te je analizirana sezonska dinamika dvanaest vrsta s najvećim brojem uzorkovanih jedinki. Na temelju sastava zajednica odraslih tulara određeni su indeksi saprobnosti, rasprostranjenost hranidbenih skupina te longitudinalna klasifi kacija staništa

Izvori: DNA barkodiranje tulara (Insecta, Trichoptera) u Hrvatskoj s napomenama o taksonomiji i konzervacijskoj biologiji

The paper provides the results of DNA barcoding based on the cytochrome c oxidase subunit 1 mitochondrial gene (mtCOI) of 110 Trichoptera specimens collected in 36 springs in the PannonianPeripannonian, central mountainous and Mediterranean part of Croatia. We barcoded 70 species from 32 genera and 15 families. The data obtained show interesting faunistic and taxonomic results, for, for example, the species Rhyacophila cabrankensis, R. balcanica, Crunoecia kempnyi, Allogmaus auricollis and emphasize the need for further faunistic research into springs, in their role as habitats with a specific and very interesting fauna. The mtCOI DNA barcoding should be included in such research, because it would enable better presentation of the results, especially regarding biodiversity, taxonomy, phylogeny and conservation biology, not just as a segment of a local but also of a global process of understanding biodiversity in a different way. The results of this study show a global need for the protection of springs, because they are specific not only as habitats, but also as localities with an interesting fauna and often endemic species of very limited distribution (for example Rhyacophila cabrankensis).U radu se prikazuju rezultati DNA barkodiranja temeljenog na mitohondrijskom genu za podjedinicu 1 citokrom c oksidaze (mtCOI), za 110 primjeraka Trichoptera prikupljenih u 36 izvora u panonsko-peripanonskom, središnje-planinskom i mediteranskom području Hrvatske. DNA barkodirano je 70 vrsta iz 32 roda i 15 porodica. U studiji se ukazuje na neke zanimljive faunističke i taksonomske rezultate, npr. za vrste Rhyacophila cabrankensis, R. balcanica, Crunoecia kempnyi, Allogmaus auricollis te potrebu daljnjih faunističkih istraživanja izvora kao staništa sa specifičnom i vrlo zanimljivom faunom. U ta istraživanja zbog kvalitetnije prezentacije rezultata, posebno u područjima bioraznolikosti, taksonomiji, filogeniji i konzervacijskoj biologiji, potrebno je uključiti i metodu DNA barkodiranja mtCOI, kao segment ne samo lokalnog, nego i globalnog procesa u spoznavanju bioraznolikosti na jedan drugačiji način. Navedeni rezultati ovog rada ukazuju na globalnu potrebu veće zaštite izvora jer su specifični ne samo kao staništa, nego vrlo često i kao područja nalaza endemskih vrsta s vrlo malim područjem rasprostranjenja (npr. Rhyacophila cabrankensis)

Springs: DNA barcoding of caddisflies (Insecta, Trichoptera) in Croatia with notes on taxonomy and conservation biology

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Rhyacophila fasciata subsp. delici Kucinic & Valladolid 2020

Rhyacophila fasciata delici Kučinić & Valladolid (ssp. nov.) This subspecies has been found widely distributed in Croatia and it is also present in Bosnia and Herzegovina. Etymology. The specific name is the genitive singular of Delić, given in honour of Dr. Antun Delić, naturalist and biologist, retired professor of the Teachers Faculty, University of Zagreb. Type material. Holotype ♂: CROATIA, Roški slap (Roški waterfall), National Park “Krka” (43.90625º N 15.975º E, 80 m a.s.l.), 10/v/19 (M. Kučinić & A. Delić) [R1, no. 4444 (TCK)]. Paratypes: 1 ♀, same locality as holotype, 19/v/19, (M. Kučinić & A. Delić) [R2, no. 4445 (TCK)]. 2 ♂, spring of the river Čabranka, 21/vi/15 (D. Cerjanec) [C1–C2, nos. 4446–4447 (MKC)] 2 ♂, same locality, 05/v/15 (D. Cerjanec) [C3, no. 4448 (MKC), C4, no. 1110 (UMBH)]. 1 ♂, spring of river Bijela rijeka, National Park “Plitvice Lakes”, 16/iii/18 (I. Sivec, M. Kučinić & S. Žalac) [BI1, no. 4449 (MKC)]. 1 ♂, Brkljača channel, Udovičić, 20/vi/15 (S. Žalac & M. Kučinić) [BR 1, no. 4450 (MKC)]. 1 ♀, tributary of river Cetina, Civljani, 07/vi/15 (M. Kučinić & A. Delić) [CT1, no. 4451 (MKC)]. 1 ♂ and 1 ♀, river Butišnica, Golubić, 06/viii/15 (A. Delić & A. Ćukušić) [BT1–BT2, nos. 4452–4453 (MKC)]. 1 ♀, Brušane, Gospić, Ličko-Senjska county, Road 25, 10-15/vii/16 (G. Galli) [BU1, ID Data number of Access Catalog 33789 (MCSN)]. 1 ♂ and 1 ♀, spring of the river Dretulja, 09/ix/14 (D. Cerjanec & M. Kučinić) [D1–D2, MNCN _ Ent 269370, MNCN _ Ent 269371 (MNCN)]. 1 ♂ and 1 ♀, Vukovića spring, river Cetina, 09/v/17 (A. Delić & M. Kučinić) [V1 – V2, MNCN _ Ent 269372, MNCN _ Ent 269373, (MNCN)]. 2 ♂, middle part of river Gacka, Otočac, 11/v/15 (D. Cerjanec & M. Kučinić) [G1–G2, MNCN _ Ent 269374, MNCN _ Ent 269375, (MNCN)]. Description of the imago. Holotype (R1): Length from front of head to distal edge of segment IX 8.82 mm, each forewing 11.42 mm, each hind wing 9.65 mm. Males: Length 7.21–9.92 mm (x = 8.61, n = 12), each forewing 10.07–13.09 (x = 11.15, n = 12), each hind wing 8.29–11.17 mm (x = 9.66, n = 12). Females: Length from front of head to distal edge of segment VIII 8.96–11.61 mm (x = 10.69, n = 6), each forewing 10.48–14.15 mm (x = 12.28, n = 6), each hind wing 9.77–12.7 mm (x = 10.93, n = 6). In ethanol-preserved specimens colour generally pale brown and yellowish, with golden brown setae. Head, thorax, and abdomen dorsally pale brown, with small black and irregular darker brown spots in dorsal area, abdomen ventrally yellowish; females generally darker than males, anterior 2/3 of abdominal segments pale brown or brown in darker specimens. Legs light, with spurs reddish brown. Forewings brown, spotted lighter than background; hind wings pale. Male genitalia (Figs 1, 5 a–5e): Apical segment of each inferior appendage (Figs 1A, 5a) with basal and distal edges diverging, posterior edge oblique straight or slightly convex, ventral edge slightly concave, at least two times longer that dorsal edge. Apicodorsal vertex slightly angular, apicoventral angle projecting as thick lobe narrowing progressively to round apex (Fig 5a). Parameres (Figs 1 BV–1BL, 5c, 5d) in ventral view curved posteromesad in apical half (Figs 1 BV p, 5c). In lateral view (Figs 1 BL, 5d) each slender at base, dilated in middle, with almost parallel dorsal and ventral margins in central area, pointed at apex; few thick spines on midventral margin and posterior midventral area, parallel to surface (Figs 5c, 5d); midlateral surface almost completely covered by thin spicules or setae, reaching from anteroventral edge to posterodorsal edge of paramere, decreasing in size from ventral to dorsal margin, absent on middorsal edge. Aedeagus (phallicata) in lateral view (Figs 1 CL, 5e) with anterodorsal margin straight, then slightly concave with posterior corner of concavity hooked anterad, upper posterior edge slightly concave, then straight caudad, ventral apex round, ventral edge curved upward anteriorly to slight angle below hook. Lateroventral lobes of phallus (Fig 1 CD lvl) convex, narrowing progressively towards apex, apicolateral margins slightly pointed to inside, posterior edge of each lobe concave. Ventral lobe of aedeagus subtriangular, pointed progressively backwards (Fig 1 BV vl). Apicodorsal lobe of segment IX (Figs 1 DD, 5b) dilated subapicolaterally, nearly circular, ratio of posterior maximum width to anterior width 2:1; preanal appendages (Fig 1 DD pa) shorter than apicodorsal lobe (Fig 1 DD al) of segment IX laterally, with concave posterior edges. In ventral view (Fig 1 DV), apical band V-shaped, inner and outer edges almost parallel in posterior third, rounded apically, longer than wide (Fig 1 DV ab); posterior edge of non-sclerotized ventral area round, with apicomesal incision (Fig 1 DV va), anal sclerites triangular (Fig 1 DV as).Published as part of Valladolid, María, Kučinić, Mladen, Arauzo, Mercedes, Cerjanec, Darko, Ćuk, Renata, Dorda, Beatriz A., Lodovici, Omar, Stanić-Koštroman, Svjetlana, Vučković, Ivan & Rey, Isabel, 2020, The Rhyacophila fasciata Group in Croatia and Bosnia and Herzegovina: Rhyacophila f. fasciata Hagen 1859 and the description of two new subspecies, Rhyacophila fasciata delici Kučinić & Valladolid (ssp. nov.) from Croatia and Bosnia and Herzegovina and Rhyacophila fasciata viteceki Valladolid & Kučinić, pp. 51-75 in Zootaxa 4885 (1) on page 60, DOI: 10.11646/zootaxa.4885.1.3, http://zenodo.org/record/429637

Rhyacophila fasciata subsp. viteceki Valladolid & Kucinic 2020

Rhyacophila fasciata viteceki Valladolid & Kučinić (ssp. nov.) This subspecies has been found in Bosnia and Herzegovina. Etymology. The specific name is the genitive of Vitecek, given in honour of Dr. Simon Vitecek, biologist and trichopterologist of Wasser Cluster Lunz, Lunz am See, Austria. Type material. Holotype ♂: BOSNIA AND HERZEGOVINA, river Neretva, Bačevići (43.254154º N 17.824627º E, 35 m a.s.l.), 30/vi/17 (Stanić-Koštroman & M. Kučinić) [N1, MNCN _ Ent 269367 (MNCN)]. Paratypes: 2 ♂, same locality as holotype, 30/vi/17 (Stanić-Koštroman & M. Kučinić) [N2, no. 4455, (MCK) and N3, no. 1111 (UMBH)]. 2 ♂ and 1 ♀, river Sturba, 30/viii/18 (M. Kučinić) [1 ♂, S1, MNCN _ Ent 269368 (MNCN); 1 ♀, S3, MNCN _ Ent 269369 (MNCN); 1 ♂ S2, no. 4456 (MKC)]. 1 ♂, river Bunica, 04/vi/17 (StanićKoštroman & M. Kučinić) [B1, no. 1112 (UMBH)]. Description of the imago. Holotype (N1): Length from front of head to distal edge of segment IX 8.91 mm, each forewing 12.55 mm, each hind wing 10.54 mm. Males: Length 8.29–9.21 mm (x = 8.72, n = 6), each forewing 9.83–12.55 (x = 11.34, n = 6), each hind wing 8.85–10.54 mm (x = 9.79, n = 6). Female: Length from front of head to distal edge of segment VIII 11.26 mm (n =1), forewing 14.52 mm (n = 1), hind wing 12.15 mm (n = 1). In ethanol-preserved specimens colour generally pale brown, yellowish, with golden brown setae. Head, thorax, and abdomen dorsally pale brown, with small dark brown spots in dorsal area, some specimens in lateral view with long black spots marking borders between dorsal and ventral areas, abdomen ventrally yellowish; female darker than male, anterior 2/3 of abdominal segments pale brown or brown in darker specimens. Legs light, with spurs reddish brown. Forewings brown, spotted; hind wings pale. Male genitalia (Figs 3, 6 a–6e): Apical segment of each inferior appendage (Figs 3A, 6a) with basal and distal edges diverging, posterior edge oblique and slightly convex, ventral edge straight to slightly concave, at least two times longer that dorsal edge. Apicodorsal vertex angular, apicoventral angle projecting as thick lobe narrowing progressively to round apex (Fig 6a). Parameres (Figs 3 BV–3BL, 6c–6d) in ventral view curved posteromesad in apical 2/3 (Figs. 3 BV p, 6c). In lateral view (Figs. 3 BL, 6d) each slender at base, slightly dilated in middle, with rounded dorsal margin before midlength and almost straight ventral margin at midlength, pointed at apex; row of few thick spines on midventral margin (Figs 6 c–6d); midlateral surface covered by very fine spicules or setae, reaching from anteroventral to posterodorsal edges of paramere, absent from dorsal edge. Aedeagus (phallicata) in lateral view (Figs 3 CL, 6e) with dorsal margin deeply concave and posterior corner of concavity hooked anterad; upper posterior edge concave to obliquely truncate apex, ventral edge sinous. Lateroventral lobes of phallus (Fig. 3 CD lvl) convex, narrowing progressively towards apex, apicolateral margins round, posteromesal edge of each lobe concave. Ventral lobe of aedeagus nearly round, tapering and acute caudally (Fig 3 BV vl). Apicodorsal lobe of segment IX (Figs 3 DD, 6b) slightly dilated subapicolaterally, posterior edge semicircular, lateral edges converging anterad; preanal appendages (fig 3DD pa) shorter than apicodorsal lobe (Fig 3 DD al), obliquely subtruncate apicolaterally, with slightly concave posteromesal edges (Figs. 3 DD, 6b). In ventral view, apical band V-shaped, inner and outer edges converging in posterior third, pointed apically, longer than wide (Fig 3 DV ab); posterior edge of non-sclerotized ventral area slightly concave, with small mesal incision (Fig 3 DV va), anal sclerites subtriangular, concave apically (Fig 3 DV as).Published as part of Valladolid, María, Kučinić, Mladen, Arauzo, Mercedes, Cerjanec, Darko, Ćuk, Renata, Dorda, Beatriz A., Lodovici, Omar, Stanić-Koštroman, Svjetlana, Vučković, Ivan & Rey, Isabel, 2020, The Rhyacophila fasciata Group in Croatia and Bosnia and Herzegovina: Rhyacophila f. fasciata Hagen 1859 and the description of two new subspecies, Rhyacophila fasciata delici Kučinić & Valladolid (ssp. nov.) from Croatia and Bosnia and Herzegovina and Rhyacophila fasciata viteceki Valladolid & Kučinić, pp. 51-75 in Zootaxa 4885 (1) on pages 65-66, DOI: 10.11646/zootaxa.4885.1.3, http://zenodo.org/record/429637

The Rhyacophila fasciata Group in Croatia and Bosnia and Herzegovina: Rhyacophila f. fasciata Hagen 1859 and the description of two new subspecies, Rhyacophila fasciata delici Kučinić & Valladolid (ssp. nov.) from Croatia and Bosnia and Herzegovina and Rhyacophila fasciata viteceki Valladolid & Kučinić (ssp. nov.) from Bosnia and Herzegovina (Trichoptera: Rhyacophilidae)

[EN] We present the description of two new subspecies of the Rhyacophila fasciata Group: Rhyacophila fasciata delici Ku¿ini¿ & Valladolid (ssp. nov.), broadly distributed in Croatia and present also in Bosnia and Herzegovina, and R. fasciata viteceki Valladolid & Ku¿ini¿ (ssp. nov.), found in Bosnia and Herzegovina. Our study of the morphology of adults, as well as our analysis of the barcode region of the mitochondrial cytochrome oxidase I (mtCOI) gene and geographical distribution confirm the differences of the two new subspecies with the nominal species R. f. fasciata, also found in both countries.[ES] Se presenta la descripción de dos nuevas subespecies del Grupo Rhyacophila fasciata: Rhyacophila fasciata delici Kučinić& Valladolid (ssp. nov.), ampliamente distribuida en Croacia y presente también en Bosnia y Herzegovina y R. fasciata vitecekiValladolid & Kučinić (ssp. nov.), encontrada en Bosnia y Herzegovina. El estudio de la morfología de los adultos, así como el análisis de la citocromo oxidasa I mitocondrial (COImit) y la distribución geográfica confirma las diferencias de las dos nuevas subespecies con la especie nominal R. f. fasciata, también presente en ambos países.This research is a part of scientific project “DNA barcoding of Croatian faunal biodiversity” (IP–06–2016–9988) funded by the Croatian Science Foundation