Utilizing CoCoRaHS Climate Data to Improve Climate Literacy at the Middle School Level

One of the biggest obstacles seen in K-12 education today is the lower scores in math and science related subjects versus language arts and historical perspectives. Due to the need to improve these scores, many different methods are being tested and reviewed to improve overall understanding and ability. It is becoming more recognized in these efforts that the use of scientific method, experimentation, data collection and measurements are fantastic ways to improve understanding in math. One commonly noted difference is that math is “just numbers” and one can often go through the motions and complete the problem, but not necessarily understand the thought behind the numbers. However, science requires understanding of the situation and applying math accordingly, a skill that carries over into basic math understanding. The improvement of the sciences would not only benefit students in their science courses, but also in math as well

A simple model for predicting snow albedo decay using observations from the Community Collaborative Rain, Hail, and Snow-Albedo (CoCoRAHS-Albedo) Network

The albedo of seasonal snow cover plays an important role in the global climate system due to its influence on Earth’s radiation budget and energy balance. Volunteer CoCoRaHS-Albedo observers collected 3,249 individual daily albedo, snow depth, and density measurements using standardized techniques at dozens of sites across New Hampshire, USA over four winter seasons. The data show that albedo increases rapidly with snow depth up to ~ 0.14 m. Multiple linear regression models using snowpack age, snow depth or density, and air temperature provide reasonable approximations of surface snow albedo during times of albedo decay. However, the linear models also reveal systematic biases that highlight an important non-linearity in snow albedo decay. Modeled albedo values are reasonably accurate within the range of 0.6 to 0.9, but exhibit a tendency to over-estimate lower albedo values and under-estimate higher albedo values. We hypothesize that rapid reduction in high albedo fresh snow results from a decrease in snow specific surface area, while during melt-events the presence of liquid water in the snowpack accelerates metamorphism and grain growth. We conclude that the CoCoRaHS-Albedo volunteer observer network provides useful snow albedo, depth, and density measurements and serves as an effective model for future measurement campaigns

Regulation of p27kip1 and p57kip2 functions by natural polyphenols

In numerous instances, the fate of a single cell not only represents its peculiar outcome but also contributes to the overall status of an organism. In turn, the cell division cycle and its control strongly influence cell destiny, playing a critical role in targeting it towards a specific phenotype. Several factors participate in the control of growth, and among them, p27Kip1 and p57Kip2, two proteins modulating various transitions of the cell cycle, appear to play key functions. In this review, the major features of p27 and p57 will be described, focusing, in particular, on their recently identified roles not directly correlated with cell cycle modulation. Then, their possible roles as molecular effectors of polyphenols’ activities will be discussed. Polyphenols represent a large family of natural bioactive molecules that have been demonstrated to exhibit promising protective activities against several human diseases. Their use has also been proposed in association with classical therapies for improving their clinical effects and for diminishing their negative side activities. The importance of p27Kip1 and p57Kip2 in polyphenols’ cellular effects will be discussed with the aim of identifying novel therapeutic strategies for the treatment of important human diseases, such as cancers, characterized by an altered control of growth

A Beckwith–Wiedemann-associated CDKN1C mutation allows the identification of a novel nuclear localization signal in human p57Kip2

p57Kip2 protein is a member of the CIP/Kip family, mainly localized in the nucleus where it exerts its Cyclin/CDKs inhibitory function. In addition, the protein plays key roles in embryogenesis, differentiation, and carcinogenesis depending on its cellular localization and interactors. Mutations of CDKN1C, the gene encoding human p57Kip2, result in the development of different genetic diseases, including Beckwith–Wiedemann, IMAGe and Silver–Russell syndromes. We investigated a specific Beckwith–Wiedemann associated CDKN1C change (c.946 C>T) that results in the substitution of the C-terminal amino acid (arginine 316) with a tryptophan (R316W-p57Kip2). We found a clear redistribution of R316W-p57Kip2, in that while the wild-type p57Kip2 mostly occurs in the nucleus, the mutant form is also distributed in the cytoplasm. Transfection of two expression constructs encoding the p57Kip2 N-and C-terminal domain, respectively, allows the mapping of the nuclear localization signal(s) (NLSs) between residues 220–316. Moreover, by removing the basic RKRLR sequence at the protein C-terminus (from 312 to 316 residue), p57Kip2 was confined in the cytosol, implying that this sequence is absolutely required for nuclear entry. In conclusion, we identified an unreported p57Kip2 NLS and suggest that its absence or mutation might be of relevance in CDKN1C-associated human diseases determining significant changes of p57Kip2 localization/regulatory roles

Evaluation of a loop-mediated isothermal amplification method for the detection of listeria monocytogenes in dairy food

Objective of the present study was to test the performances of a loop-mediated isothermal amplification (LAMP)-based method for the detection of Listeria monocytogenes, with particular focus on the dairy products. The specificity of the method was evaluated on 42 different Listeria spp. strains from collections, food and environmental samples. 100% (32 of 32) of the L. monocytogenes strains were correctly recognised, and none of other 10 Listeria spp. strains was misidentified. The sensitivity was evaluated on four L. monocytogenes strains from different sources. The instrument was able to detect 10-400 CFU/mL. The ability to detect low initial numbers of L. monocytogenes (0.3- 0.7 Log CFU/g) was also evaluated, in duplicate, in pasteurised milk (whole and skimmed) and dairy samples (fresh ricotta, crescenza, mascarpone, mozzarella, cottage cheese, cream cheese, taleggio, gorgonzola). The analysis was performed after 18, 24 and 48 h of incubation, and was coupled with the count of L. monocytogenes in the broth. Microbial loads were insufficient to achieve a positive result after 18 and 24 h in most of the samples; after 48 h, all the products, except taleggio and one gorgonzola sample, were identified as positive; the sensitivity of the method when applied to contaminated dairy foods was about 5 Log CFU/g. The LAMP method tested can be considered a very useful tool, as it is a costeffective and easy-functioning method. The preliminary data obtained should be confirmed with a validation process taking into account different food typologies

Evaluation of a New Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Anisakis spp.

Objective of the present study was to test the performances of a real-time LAMP-based field- friendly tool system for the detection of Anisakis spp., with particular focus on fish products. The specificity of the method was evaluated on Anisakis spp. larvae from internal collection. 100% of the Anisakis spp. strains tested were recognized, while, correctly, no amplification occurred for non-pathogenic Hysterothylacium spp. The sensitivity was evaluated in three independent trails conducted on intentionally infested at several intensities salmon fish fillets homogenate, on seabream commercial baby food and on domestic seabream baby food. Results obtained showed a detected minimum intensity of 20 larvae/kg in the first trial (infected salmon fillets homogenate), while in intentionally infected commercial and homemade seabream baby foods this minimum intensity was 4 larvae/kg, in agreement with the limit suggested by the Codex Alimentarius for instruments intended for the identification of the presence of larvae in fishery products (5 larvae/kg). The system did not give the same performances in the equivalent matrixes after thermal treatment inactivation. This LAMP method can be considered a very useful tool for the application to fish raw matrixes as it is a cost-effective and easy-functioning method, while in the detection of inactivated larvae for the prevention of possible allergic reactions, other studies should be performed

An update of the evolving epidemic of blaKPC carrying Klebsiella pneumoniae in Sicily, Italy, 2014: Emergence of multiple Non-ST258 Clones

Background: In Italy, Klebsiella pneumoniae carbapenemase producing K. pneumoniae (KPC-Kp) strains are highly endemic and KPC producing CC258 is reported as the widely predominating clone. In Palermo, Italy, previous reports have confirmed this pattern. However, recent preliminary findings suggest that an epidemiological change is likely ongoing towards a polyclonal KPC-Kp spread. Here we present the results of molecular typing of 94 carbapenem non susceptible K. pneumoniae isolates detected during 2014 in the three different hospitals in Palermo, Italy. Methods and Results: Ninety-four consecutive, non replicate carbapenem non susceptible isolates were identified in the three largest acute general hospitals in Palermo, Italy, in the six-month period March-August 2014. They were characterized by PCR for β-lactam, aminoglycoside and plasmid mediated fluoroquinolone resistance genetic determinants. The mgrB gene of the colistin resistant isolates was amplified and sequenced. Clonality was assessed by pulsed field gel electrophoresis and multilocus sequence typing. Eight non-CC258 sequence types (STs) were identified accounting for 60% of isolates. In particular, ST307 and ST273 accounted for 29% and 18% of isolates. CC258 isolates were more frequently susceptible to gentamicin and non-CC258 isolates to amikacin. Colistin non susceptibility was found in 42% of isolates. Modifications of mgrB were found in 32 isolates. Conclusions: Concurrent clonal expansion of some STs and lateral transmission of genetic resistance determinants are likely producing a thorough change of the KPC-Kp epidemiology in Palermo, Italy. In our setting mgrB inactivation proved to substantially contribute to colistin resistance. Our findings suggest the need to continuously monitor the KPC-Kp epidemiology and to assess by a nationwide survey the possible shifting towards a polyclonal epidemic

An update of the evolving epidemic of blaKPC carrying Klebsiella pneumoniae in Sicily, Italy, 2014: Emergence of multiple Non-ST258 Clones

Background: In Italy, Klebsiella pneumoniae carbapenemase producing K. pneumoniae (KPC-Kp) strains are highly endemic and KPC producing CC258 is reported as the widely predominating clone. In Palermo, Italy, previous reports have confirmed this pattern. However, recent preliminary findings suggest that an epidemiological change is likely ongoing towards a polyclonal KPC-Kp spread. Here we present the results of molecular typing of 94 carbapenem non susceptible K. pneumoniae isolates detected during 2014 in the three different hospitals in Palermo, Italy. Methods and Results: Ninety-four consecutive, non replicate carbapenem non susceptible isolates were identified in the three largest acute general hospitals in Palermo, Italy, in the six-month period March-August 2014. They were characterized by PCR for β-lactam, aminoglycoside and plasmid mediated fluoroquinolone resistance genetic determinants. The mgrB gene of the colistin resistant isolates was amplified and sequenced. Clonality was assessed by pulsed field gel electrophoresis and multilocus sequence typing. Eight non-CC258 sequence types (STs) were identified accounting for 60% of isolates. In particular, ST307 and ST273 accounted for 29% and 18% of isolates. CC258 isolates were more frequently susceptible to gentamicin and non-CC258 isolates to amikacin. Colistin non susceptibility was found in 42% of isolates. Modifications of mgrB were found in 32 isolates. Conclusions: Concurrent clonal expansion of some STs and lateral transmission of genetic resistance determinants are likely producing a thorough change of the KPC-Kp epidemiology in Palermo, Italy. In our setting mgrB inactivation proved to substantially contribute to colistin resistance. Our findings suggest the need to continuously monitor the KPC-Kp epidemiology and to assess by a nationwide survey the possible shifting towards a polyclonal epidemic

Patterns and Perceptions of Climate Change in a Biodiversity Conservation Hotspot

Quantifying local people's perceptions to climate change, and their assessments of which changes matter, is fundamental to addressing the dual challenge of land conservation and poverty alleviation in densely populated tropical regions To develop appropriate policies and responses, it will be important not only to anticipate the nature of expected changes, but also how they are perceived, interpreted and adapted to by local residents. The Albertine Rift region in East Africa is one of the world's most threatened biodiversity hotspots due to dense smallholder agriculture, high levels of land and resource pressures, and habitat loss and conversion. Results of three separate household surveys conducted in the vicinity of Kibale National Park during the late 2000s indicate that farmers are concerned with variable precipitation. Many survey respondents reported that conditions are drier and rainfall timing is becoming less predictable. Analysis of daily rainfall data for the climate normal period 1981 to 2010 indicates that total rainfall both within and across seasons has not changed significantly, although the timing and transitions of seasons has been highly variable. Results of rainfall data analysis also indicate significant changes in the intra-seasonal rainfall distribution, including longer dry periods within rainy seasons, which may contribute to the perceived decrease in rainfall and can compromise food security. Our results highlight the need for fine-scale climate information to assist agro-ecological communities in developing effective adaptive management

Loop-mediated isothermal amplification (LAMP) for the detection of Anisakis simplex

Rapid identification of Anisakis spp. larvae is very interesting for fish food industry. Nevertheless, biomolecular techniques are labour-intensive and time-consuming, and they require technical know-how and expensive equipment. Loop-mediated isothermal amplification (LAMP) is a relatively new technique and it proposes significant advantages in terms of ease and speed; moreover, it has already been developed and extensively used as a diagnostic tool for some pathogenic bacteria sush as Listeria monocytogenes and parasites like Toxoplasma gondii [1, 2]. In this study, a specific primer was designed and a fluorescence-based real-time LAMP assay was studied, providing an alternative and primarily screening approach for the identification of the genus Anisakis. The primer developed was tested directly against Anisakis spp. and other non-pathogenic Anisakidae larvae (Hysterothylacium fabri). Subsequently, the kit was tested against homogenized fish muscle voluntarily contaminated with a decreasing number of larvae, previously isolated and identified to genus level. Finally, baby food samples (80g) contaminated with one, two and three larvae were also tested. The specificity of the kit developed to amplify DNA sequences belonging to Anisakis spp. was confirmed as larvae belonging to Anisakis simplex sensu strictu and A. pegreffii were amplified and recognized by the kit, while Hysterothylacium fabri, a non zoonotic parasite morphologically similar to Anisakis spp. and belonging to the same family, was not recognized. The tests performed using mixed fish muscle in order to evaluate the sensitivity of the kit resulted to be in agreement with the biomolecular tecniques actually used [3, 4] with a sensitivity equal to 20 larvae/kg of product. Comparable sensitivity was also confirmed with the test perfomed using baby food [5]. LAMP tecnique applied could have promising practical outcomes as it is able to produce good identification results in short times and with low costs. Important advantages could be especially produced if applied to the periodical control of fish-based products (like baby food) or to quality control of extensive productions before commercialization