Short-Term Daily Emergence of Adult Midges (Diptera: Chironomidae) from a Natural Lake and an Artificial Reservoir

Daily emergence of adult Chironomidae in a lake and an artificial reservoir in Florida was sampled at 24-h intervals during spring and summer 1980 and summer 1981. Changes in water temperature during the sampling periods were recorded. Linear population trends of a species with time were examined by multiple linear regression. Temporal relationships of emergence periodicity within a species, between species, and between a catch of a species and daily water temperature were examined by autocorrelation and standard correlation analyses. Glyptotendipes paripes Edwards and Chironomus decorus Johannsen in the reservoir, and G. paripes and C. crassicaudatus Malloch in the lake, were quantitatively important and emerged on a daily basis at 18.5 to 30.0°C water temperatures. Trends of adult emergence of all species in relation to Julian day were inconsistent. Some significant autocorrelations and standard correlations existed, but all the correlation coefficient values were less than 0.66. From the daily emergence of the species studied, it can be suggested that their oviposition in central Florida lakes takes place almost on a daily basis at 18.0 to 30.0°C water temperatures, and several asynchronous generations of each species develop resulting in their asynchronous emergenc

Statistical methods for analyzing immunosignatures

<p>Abstract</p> <p>Background</p> <p>Immunosignaturing is a new peptide microarray based technology for profiling of humoral immune responses. Despite new challenges, immunosignaturing gives us the opportunity to explore new and fundamentally different research questions. In addition to classifying samples based on disease status, the complex patterns and latent factors underlying immunosignatures, which we attempt to model, may have a diverse range of applications.</p> <p>Methods</p> <p>We investigate the utility of a number of statistical methods to determine model performance and address challenges inherent in analyzing immunosignatures. Some of these methods include exploratory and confirmatory factor analyses, classical significance testing, structural equation and mixture modeling.</p> <p>Results</p> <p>We demonstrate an ability to classify samples based on disease status and show that immunosignaturing is a very promising technology for screening and presymptomatic screening of disease. In addition, we are able to model complex patterns and latent factors underlying immunosignatures. These latent factors may serve as biomarkers for disease and may play a key role in a bioinformatic method for antibody discovery.</p> <p>Conclusion</p> <p>Based on this research, we lay out an analytic framework illustrating how immunosignatures may be useful as a general method for screening and presymptomatic screening of disease as well as antibody discovery.</p

Attraction of Adult Chironomidae (Diptera) to Incandescent Light Under Laboratory Conditions

The attraction of three chironomid species, Glyptotendipes paripes Edwards, Chironomus crassicaudatus Malloch, and Polypedilum halterale (Coquillett), to incandescent light of different colors and wattages was studied. Field-captured adults were released from the center of a dark room (9 by 9 m) equipped with a New Jersey light trap in each corner. The effects of color were determined by using 100-W lamps of red, orange, yellow, green, blue, and white. The effects of light intensity were studied by using 100, 60, 40, and 25-W white lamps. Measurements of light intensity of each lamp were taken. Among the colors tested in two separate combinations, white light attracted the maximum numbers of adults and red light the least. The three species exhibited a similar behavior. Among white light of different intensities, the maximum attraction of G. paripes occurred toward the highest intensity and the minimum toward the lowest intensity. The midge species responded more to the quantity (power or intensity) than to the quality (color or wavelength) of ligh

Physical Conditions in Circumstellar Gas surrounding SN 1987A 12 Years After Outburst

Two-dimensional spectra of Supernova 1987A were obtained on 1998 November 14-15 (4282 days after outburst) with the Space Telescope Imaging Spectrograph (STIS) on board the Hubble Space Telescope (HST). The slit sampled portions of the inner circumstellar ring at the east and west ansae as well as small sections of both the northern and southern outer rings. The temperature and density at these locations are estimated by nebular analysis of [N II], [O III], and [S II] emission line ratios, and with time-dependent photoionization/recombination models. The results from these two methods are mutually consistent. The electron density in the inner ring is ~ 4000 cm-3 for S II, with progressively lower densities for N II and O III. The electron temperatures determined from [N II] and [O III] line ratios are ~11,000 K and \~22,000 K, respectively. These results are consistent with evolutionary trends in the circumstellar gas from similar measurements at earlier epochs. We find that emission lines from the outer rings come from gas of lower density (n_e \la 2000 cm-3) than that which emits the same line in the inner ring. The N/O ratio appears to be the same in all three rings. Our results also suggest that the CNO abundances in the northern outer ring are the same as in the inner ring, contrary to earlier results of Panagia et al. (1996). Physical conditions in the southern outer ring are less certain because of poorer signal-to-noise data. The STIS spectra also reveal a weak Ha emission redshifted by ~100 km s-1 at p.a. 103\arcdeg that coincides with the recently discovered new regions that are brightening (Lawrence et al. 2000). This indicates that the shock interaction in the SE section of the inner ring commenced over a year before it became apparent in HST images.Comment: 25 pages, 6 figures, to appear in December 1, 2000 Astrophysical Journa

A DedA Family Membrane Protein Is Required for Burkholderia thailandensis Colistin Resistance

© Copyright © 2019 Panta, Kumar, Stafford, Billiot, Douglass, Herrera, Trent and Doerrler. Colistin is a “last resort” antibiotic for treatment of infections caused by some multidrug resistant Gram-negative bacterial pathogens. Resistance to colistin varies between bacterial species. Some Gram-negative bacteria such as Burkholderia spp. are intrinsically resistant to very high levels of colistin with minimal inhibitory concentrations (MIC) often above 0.5 mg/ml. We have previously shown DedA family proteins YqjA and YghB are conserved membrane transporters required for alkaline tolerance and resistance to several classes of dyes and antibiotics in Escherichia coli. Here, we show that a DedA family protein in Burkholderia thailandensis (DbcA; DedA of Burkholderia required for colistin resistance) is a membrane transporter required for resistance to colistin. Mutation of dbcA results in \u3e100-fold greater sensitivity to colistin. Colistin resistance is often conferred via covalent modification of lipopolysaccharide (LPS) lipid A. Mass spectrometry of lipid A of ΔdbcA showed a sharp reduction of aminoarabinose in lipid A compared to wild type. Complementation of colistin sensitivity of B. thailandensis ΔdbcA was observed by expression of dbcA, E. coli yghB or E. coli yqjA. Many proton-dependent transporters possess charged amino acids in transmembrane domains that take part in the transport mechanism and are essential for function. Site directed mutagenesis of conserved and predicted membrane embedded charged amino acids suggest that DbcA functions as a proton-dependent transporter. Direct measurement of membrane potential shows that B. thailandensis ΔdbcA is partially depolarized suggesting that loss of protonmotive force can lead to alterations in LPS structure and severe colistin sensitivity in this species

Beluga whales in the western Beaufort Sea : current state of knowledge on timing, distribution, habitat use and environmental drivers

ECG was supported by a National Research Council-National Academy of Sciences Postdoctoral Fellowship.The seasonal and geographic patterns in the distribution, residency, and density of two populations (Chukchi and Beaufort) of beluga whales (Delphinapterus leucas) were examined using data from aerial surveys, passive acoustic recordings, and satellite telemetry to better understand this arctic species in the oceanographically complex and changing western Beaufort Sea. An aerial survey data-based model of beluga density highlights the Beaufort Sea slope as important habitat for belugas, with westerly regions becoming more important as summer progresses into fall. The Barrow Canyon region always had the highest relative densities of belugas from July-October. Passive acoustic data showed that beluga whales occupied the Beaufort slope and Beaufort Sea from early April until early November and passed each hydrophone location in three broad pulses during this time. These pulses likely represent the migrations of the two beluga populations: the first pulse in spring being from Beaufort animals, the second spring pulse Chukchi belugas, with the third, fall pulse a combination of both populations. Core-use and home range analyses of satellite-tagged belugas showed similar use of habitats as the aerial survey data, but also showed that it is predominantly the Chukchi population of belugas that uses the western Beaufort, with the exception of September when both populations overlap. Finally, an examination of these beluga datasets in the context of wind-driven changes in the local currents and water masses suggests that belugas are highly capable of adapting to oceanographic changes that may drive the distribution of their prey.PostprintPeer reviewe

The effect of nutrition and body condition of triplet-bearing ewes during late pregnancy on the behaviour of ewes and lambs

Objective Triplet-born lambs are less likely to survive to weaning than twin-born or single-born lambs. Appropriate ewe-lamb bonding behaviours and lamb vigour behaviours are necessary for survival of lambs. The aim of this experiment was to determine whether maternal nutrition during late pregnancy influenced behaviour of the ewe and her lambs soon after birth, and to determine whether mid-pregnancy body condition score (BCS) influenced any behavioural response. Methods The experiments included ewes that were in BCS 2.0, 2.5, or 3.0 in mid-pregnancy and were fed either ad libitum or to pregnancy-maintenance requirements in late-pregnancy (day 115 until 136 in experiment one, and day 128 until 141 in experiment two). The time taken for lambs to stand, contact dam, suck from dam and follow dam was recorded three to 18 h after birth. The number of high- and low-pitched bleats emitted by the ewe and lambs was recorded, along with maternal behaviour score (MBS) of the ewe. Lambs in experiment two underwent a maternal-recognition test at 12 or 24 h. Results There were significant effects of feeding treatment on bleating behaviour of ewes and lambs, but these were inconsistent among BCS groups and between experiments. Lamb vigour behaviours were not affected by feeding treatment. In experiment one, there was no effect of feeding treatment or BCS on MBS, but in experiment two, ewes in BCS3 in mid-pregnancy had greater MBS than ewes in BCS2 in mid-pregnancy (MBS 3.1/5 vs MBS 2.1/5; p<0.05). Conclusion Given there were no repeatable effects on behaviour of ewes and lambs, ad libitum feeding rather than feeding for pregnancy-maintenance requirements cannot be used to improve behaviours soon after birth of triplet-bearing ewes in BCS 2–3 and their lambs in extensive pastoral conditions

Ecosystem Stewardship: Sustainability Strategies for a Rapidly Changing Planet

Ecosystem stewardship is an action-oriented framework intended to foster social-ecological sustainability of a rapidly changing planet. Recent developments identify three strategies that make optimal use of current understanding in an environment of inevitable uncertainty and abrupt change: reducing the magnitude of, and exposure and sensitivity to, known stresses; focusing on proactive policies that shape change; and avoiding or escaping unsustainable social-ecological traps. All social-ecological systems are vulnerable to recent and projected changes but have sources of adaptive capacity and resilience that can sustain ecosystem services and human well-being through active ecosystem stewardship

A four-helix bundle stores copper for methane oxidation

Methane-oxidising bacteria (methanotrophs) require large quantities of copper for the membrane-bound (particulate) methane monooxygenase (pMMO). Certain methanotrophs are also able to switch to using the iron-containing soluble MMO (sMMO) to catalyse methane oxidation, with this switchover regulated by copper. MMOs are Nature’s primary biological mechanism for suppressing atmospheric levels of methane, a potent greenhouse gas. Furthermore, methanotrophs and MMOs have enormous potential in bioremediation and for biotransformations producing bulk and fine chemicals, and in bioenergy, particularly considering increased methane availability from renewable sources and hydraulic fracturing of shale rock. We have discovered and characterised a novel copper storage protein (Csp1) from the methanotroph Methylosinus trichosporium OB3b that is exported from the cytosol, and stores copper for pMMO. Csp1 is a tetramer of 4-helix bundles with each monomer binding up to 13 Cu(I) ions in a previously unseen manner via mainly Cys residues that point into the core of the bundle. Csp1 is the first example of a protein that stores a metal within an established protein-folding motif. This work provides a detailed insight into how methanotrophs accumulate copper for the oxidation of methane. Understanding this process is essential if the wide-ranging biotechnological applications of methanotrophs are to be realised. Cytosolic homologues of Csp1 are present in diverse bacteria thus challenging the dogma that such organisms do not use copper in this location

Association of Accelerometry-Measured Physical Activity and Cardiovascular Events in Mobility-Limited Older Adults: The LIFE (Lifestyle Interventions and Independence for Elders) Study.

BACKGROUND:Data are sparse regarding the value of physical activity (PA) surveillance among older adults-particularly among those with mobility limitations. The objective of this study was to examine longitudinal associations between objectively measured daily PA and the incidence of cardiovascular events among older adults in the LIFE (Lifestyle Interventions and Independence for Elders) study. METHODS AND RESULTS:Cardiovascular events were adjudicated based on medical records review, and cardiovascular risk factors were controlled for in the analysis. Home-based activity data were collected by hip-worn accelerometers at baseline and at 6, 12, and 24&nbsp;months postrandomization to either a physical activity or health education intervention. LIFE study participants (n=1590; age 78.9±5.2 [SD] years; 67.2% women) at baseline had an 11% lower incidence of experiencing a subsequent cardiovascular event per 500&nbsp;steps taken per day based on activity data (hazard ratio, 0.89; 95% confidence interval, 0.84-0.96; P=0.001). At baseline, every 30&nbsp;minutes spent performing activities ≥500&nbsp;counts per minute (hazard ratio, 0.75; confidence interval, 0.65-0.89 [P=0.001]) were also associated with a lower incidence of cardiovascular events. Throughout follow-up (6, 12, and 24&nbsp;months), both the number of steps per day (per 500&nbsp;steps; hazard ratio, 0.90, confidence interval, 0.85-0.96 [P=0.001]) and duration of activity ≥500&nbsp;counts per minute (per 30&nbsp;minutes; hazard ratio, 0.76; confidence interval, 0.63-0.90 [P=0.002]) were significantly associated with lower cardiovascular event rates. CONCLUSIONS:Objective measurements of physical activity via accelerometry were associated with cardiovascular events among older adults with limited mobility (summary score &gt;10 on the Short Physical Performance Battery) both using baseline and longitudinal data. CLINICAL TRIAL REGISTRATION:URL: http://www.clinicaltrials.gov. Unique identifier: NCT01072500