Показники протеїн-вуглеводного обміну за гепатостеатозу

It is known that under the conditions of alcoholic intoxication in the blood of rats, the activity of lactate dehydrogenase, aspartate and alanine aminotransferases, together with a probable increase in the content of malic dialdehyde in hepatocytes and decrease in the activity of superoxide dismutase and catalase, is likely to increase. Such changes are evidence of metabolic disorders in the organism of animals. Hence, this work is devoted to the study of changes in some parameters of protein-carbohydrate metabolism in blood of rats with alcohol-induced hepatic steatosis and with increased carbohydrate loading.Experiments were conducted on 3 groups of male rats, each containing 5 animals. The first (control) group of rats was kept for the standard rodent diet, 2nd – with the addition of ethanol solution (30% v/v) at 8 g/kg live weight per os, and 3rd – a mixture of carbohydrates (at a final concentration 35%) was added to the alcoholic solution.In blood serum of rats, under conditions of hepatic steatosis, elevated levels of glucogenic amino acids (alanine, glutamate and glutamine) were found which were intensively used in various processes of the organism and, especially, in the glucose-alanine cycle. In animals with increased carbohydrate loading in the diet (3rd experimental group), chronic hyperglycemia has been identified, which may cause the development of pre-diabetes. Відомо, що за умов алкогольної інтоксикації у крові щурів вірогідно зростають активність лактатдегідрогенази, аспартат- і аланінамінотрансфераз разом із вірогідним збільшенням у гепатоцитах вмісту малонового діальдегіду та зниження активності супероксиддисмутази й каталази. Такі зміни свідчать про порушення метаболічних процесів у організмі тварин. Звідси, дана робота присвячена вивченню змін деяких показників білково-вуглеводного обміну в крові щурів із алкоголь-індукованим гепатостетозом та із посиленим вуглеводним навантаженням.Досліди були проведені на 3 групах щурів-самців по 5 тварин у кожній. Перша (контрольна) група щурів утримувалась за стандартного раціону для гризунів, 2 – з доаванням розчину етанолу (30% v/v) по 8 г/кг живої маси per os, а 3 – до алкогольного розчину була додана суміш вуглеводів (у кінцевій концентрації 35%).У сироватці крові щурів за умов розвитку гепатостеатозу було виявлено підвищений вміст глюкогенних амінокислот (аланіну, глутамату і глутаміну), які інтенсивно використовувалися в різних процесах організму і, особливо, в глюкозо-аланіновому цикл. У тварин з посиленим вуглеводним навантаженням у дієті (3 дослідна група) було виявлено хронічну гіперглікемію, яка може викликати розвиток переддіабету.

North American precipitation isotope (δ18O) zones revealed in time series modeling across Canada and northern United States

Delineating spatial patterns of precipitation isotopes ("isoscapes") is becoming increasingly important to understand the processes governing the modern water isotope cycle and their application to migration forensics, climate proxy interpretation, and ecohydrology of terrestrial systems. However, the extent to which these patterns can be empirically predicted across Canada and the northern United States has not been fully articulated, in part due to a lack of time series precipitation isotope data for major regions of North America. In this study, we use multiple linear regressions of CNIP, GNIP, and USNIP observations alongside climatological variables, teleconnection indices, and geographic indicators to create empirical models that predict the δ18O of monthly precipitation (δ18Oppt) across Canada and the northern United States. Five regionalization approaches are used to separate the study domain into isotope zones to explore the effect of spatial grouping on model performance. Stepwise regression-derived parameterizations quantified by permutation testing indicate the significance of precipitable water content and latitude as predictor variables. Within the Canadian Arctic and eastern portion of the study domain, models from all regionalizations capture the interannual and intraannual variability of δ18Oppt. The Pacific coast and northwestern portions of the study domain show less agreement between models and poorer model performance, resulting in higher uncertainty in simulations throughout these regions. Long-term annual average δ18Oppt isoscapes are generated, highlighting the uncertainty in the regionalization approach as it compounds over time. Additionally, monthly time series simulations are presented at various locations, and model structure uncertainty and 90% bootstrapped prediction bounds are detailed for these predictions. Key Points: Empirical models are developed to simulate 18O of monthly precipitation Precipitable water content describes the most variance in precipitation 18O Uncertainty in modeling monthly and long-term precipitation 18O is assesse

An introduction to the co-creation of policy briefs with youth and academic teams

Drawing on insights from a four-day online workshop, which explored geo-engineering and policy making with 13 youth participants, an academic and youth authorial team provide a guide to the co-creation of policy briefs. Drawing on excerpts from the policy brief at different stages of development and commentary provided by the authors during the workshops, we set out four stages including (1) Identifying the key message and audience, (2) Reading and critically engaging with examples of policy briefs during the drafting process, (3) Developing the policy brief text, and (4) Reviewing and revising the policy brief. We have developed this guidance with a co-creative, group work approach in mind and suggest that this has relevance for those working in and beyond the discipline of geography

Factors that impact on access to water and sanitation for older adults and people with disability in rural South Africa: An occupational justice perspective

Limited access to water and sanitation is a risk to health, dignity, and ability to engage in occupations. This article aims to: 1) discuss the current and historical factors affecting access to water and sanitation in rural South Africa, and 2) explore the occupational implications of water access, particularly for older adults and people with disability in rural South Africa. A literature review was carried out through searching JSTOR, Scopus, and MEDLINE databases and using framework analysis to interpret the retrieved documents. This paper also reports a thematic analysis of semi-structured interviews, conducted in 2012 in a rural area of South Africa. Environmental, political, social-economic and attitudinal factors were identified as impacting water access and occupation, in both the documentary analysis and the semi-structured interviews. Due to South Africa’s history, injustice has occurred in the forms of occupational apartheid and occupational deprivation. We argue that supply systems must enable people to easily access more water than is essential for survival, so that people can participate in meaningful and productive occupations. Therefore, access to water should be considered part of an occupational right. Recognising this right will be an integral step in ensuring that water supplies are improved to support better livelihoods, and to achieve economic and social empowerment, and quality of life for all, in line with many of the United Nations’ new Sustainable Development Goals

Great Lakes Runoff Intercomparison Project Phase 3: Lake Erie (GRIP-E)

Hydrologic model intercomparison studies help to evaluate the agility of models to simulate variables such as streamflow, evaporation, and soil moisture. This study is the third in a sequence of the Great Lakes Runoff Intercomparison Projects. The densely populated Lake Erie watershed studied here is an important international lake that has experienced recent flooding and shoreline erosion alongside excessive nutrient loads that have contributed to lake eutrophication. Understanding the sources and pathways of flows is critical to solve the complex issues facing this watershed. Seventeen hydrologic and land-surface models of different complexity are set up over this domain using the same meteorological forcings, and their simulated streamflows at 46 calibration and seven independent validation stations are compared. Results show that: (1) the good performance of Machine Learning models during calibration decreases significantly in validation due to the limited amount of training data; (2) models calibrated at individual stations perform equally well in validation; and (3) most distributed models calibrated over the entire domain have problems in simulating urban areas but outperform the other models in validation

An introduction to the co-creation of policy briefs with youth and academic teams

Drawing on insights from a four-day online workshop, which explored geo-engineering and policy making with 13 youth participants, an academic and youth authorial team provide a guide to the co-creation of policy briefs. Drawing on excerpts from the policy brief at different stages of development and commentary provided by the authors during the workshops, we set out four stages including (1) Identifying the key message and audience, (2) Reading and critically engaging with examples of policy briefs during the drafting process, (3) Developing the policy brief text, and (4) Reviewing and revising the policy brief. We have developed this guidance with a co-creative, group work approach in mind and suggest that this has relevance for those working in and beyond the discipline of geography