Monitoring nonenzymatic glycation of human immunoglobulin G by methylglyoxal and glyoxal: A spectroscopic study

The accumulation of dicarbonyl compounds, methylglyoxal (MG) and glyoxal (G), has been observed in diabetic conditions. They are formed from nonoxidative mechanisms in anaerobic glycolysis and lipid peroxidation, and they act as advanced glycation endproduct (AGE) precursors. The objective of this study was to monitor and characterize the AGE formation of human immunoglobulin G (hIgG) by MG and G using ultraviolet (UV) and fluorescence spectroscopy, circular dichroism (CD), and matrix-assisted laser desorption/ionization–mass spectrometry (MALDI–MS). hIgG was incubated over time with MG and G at different concentrations. Formation of AGE was monitored by UV and fluorescence spectroscopy. The effect of AGE formation on secondary structure of hIgG was studied by CD. Comparison of AGE profile for MG and G was performed by MALDI–MS. Both MG and G formed AGE, with MG being nearly twice as reactive as G. The combination of these techniques is a convenient method for evaluating and characterizing the AGE proteins

Stock structure analysis of Nemipterus bipunctatus (Valenciennes, 1830) from three locations along the Indian coast

1888-1895Present study was done to identify the occurrence of various stocks of Nemipterus bipunctatus along the Indian coast, based on their body and skull shape morphometrics. Fish samples were collected from three locations along the Indian coast viz. Chennai along the East coast and Mumbai and Veraval on the West coast. Twenty truss distances from nine-point truss network of body and twenty-one truss distances from eleven-point truss network of the skull were measured from each fish sample. The canonical discriminant analysis showed that the truss distances belong to the anterior region and caudal peduncle of body and olfactory region of skull were significant in separating the fish stocks. The artificial neural network analysis revealed 91.4 % and 86.14 % well classification of the specimen, based on the truss distances of body and skull respectively. The results from the study indicated that there is a significant difference among the stocks of N. bipunctatus

Economic loss assessment on juvenile fish catch due to forced non-selectivity in a selective fishing gear, gillnet along Mumbai coast, India

A study on quantity and value of juvenile fish landings was carried out in the gillnet fishery of three selected landing centers along Mumbai coast viz., Versova, Cuff Parade and Mahim in India using Out board motors (OBM), Inboard motors (IBM) and non-motorised gillnetters respectively. The data on the quantity and value of landed juveniles were collected and analysed to reach a consensus on the gross economic loss on account of juvenile fishing. A bio-economic model was used to estimate economic loss due to juvenile fishing of 18 commercially important species of finfish and shellfish. A huge economic loss was recorded due to fishing of juveniles of 18 species by three different gillnet sectors. The analysis indicated that IBM gillnetters at Cuff Parade incurred maximum loss of Rs. 62.26 crores with major contribution from juveniles of seerfish followed by non-motorised gillnetter (Rs.29.98 crores) at Mahim and 25.33 crores in OBM gillnetters at Versova

Study to assess the prevalence of human leukocyte antigen-A*3101 allele among Indian epileptic patients and its influence on safety and efficacy of antiepileptic therapy

Background: The objective was to study the prevalence of human leukocyte antigen (HLA)-A*3101 allele among epileptic patients and to assess the safety and efficacy of antiepileptic therapy.Methods: 295 subjects were selected and divided into two groups, Group I had 192 epileptic patients and Group II had 103 normal healthy controls. After written informed consent, 30 ml of mouthwash sample was collected from each subject and DNA was extracted by standard salting-out technique and used for HLA-A*3101 genotyping by two-step nested allele-specific polymerase chain reaction amplification and agarose gel electrophoresis.Results: In Group I, 12 (6.25%) of the 192 patients were tested positive for HLA-A*3101 allele and all were taking carbamazepine (CBZ). Among them, 56 (30%) subjects had developed less severe adverse effects such as headache and giddiness, skin rashes and memory disturbances, and HLA-A*3101 was present in 8 of them while 136 had no adverse effects in which 4 of them were tested positive for the allele. In Group II, 3 (2.9%) of the 103 healthy controls were tested positive for the allele. No difference was found in response to antiepileptic therapy between allele positive and negative patients.Conclusion: The present study had shown that HLA-A*3101 is prevalent in 6.25% of the Indian epileptic population under study. The presence of this allele has a significant association with the development of mild cutaneous reactions like skin rashes. However, no difference was observed in allele positive patients in response to antiepileptic therapy in comparison with allele negative patients

Elevation of conjunctival epithelial CD45INTCD11b⁺CD16⁺CD14⁻ neutrophils in ocular Stevens-Johnson syndrome and toxic epidermal necrolysis

PURPOSE. Ocular complications related to Stevens-Johnson Syndrome (SJS)–Toxic Epidermal Necrolysis (TEN) may persist and progress after resolution of systemic disease. This is thought to be related in part to persistent ocular innate-immune signaling. In this study, our aim was to characterize infiltrative conjunctival cellular profiles during acute (<12 months) and chronic (>12 months) disease. METHODS. Consecutive patients presenting with SJS-TEN over a 12-month period were followed for 1 year. Detailed clinical examination and conjunctival impression cell recovery was analyzed by flow cytometry for the presence of intraepithelial leukocytes and compared with healthy controls (n = 21). RESULTS. Ten patients were recruited of whom six had acute disease and five were classified as TEN (SCORTEN = 1, n = 4). Conjunctival inflammation was graded as absent/mild in a total of nine patients; but despite this, evidence of fornix shrinkage was observed in nine subjects. This inversely correlated with disease duration (P < 0.05). A reduction in percentage of CD8αβ(+) T cells compared with controls (80% vs. 57%; P < 0.01) was associated with a corresponding increase in the number/percentage of CD45(INT)CD11b(+)CD16(+)CD14(−) neutrophils (186 vs. 3.4, P < 0.01, 31% vs. 0.8%, P < 0.001). Neutrophils inversely correlated with disease duration (r = −0.71, P = 0.03), yet there was no absolute change in the CD8αβ(+) or neutrophil populations during the study period (P = 1.0). CONCLUSIONS. These data highlight that a neutrophilic infiltrate is present in mildly inflamed or clinically quiescent conjunctival mucosa in patients with ocular SJS-TEN, where neutrophil numbers inversely correlate with disease duration. Neutrophil persistence endorses the hypothesis of an unresolved innate-inflammatory process that might account for disease progression

Differentiation in morphometric traits of Chanos chanos stocks along the Indian coast

233-238Stocks of Chanos chanos (Milkfish) are identified based on the morphometric traits from four different locations from the Indian coast. A total of 246 fish samples were collected for the present study from the East coast (Mandapam Lagoon and Chilika Lake) and West coast (Mandovi–Zuari estuarine system and Cochin backwaters). Digital imaging techniques were employed to extract 18 morphometric characters from the specimens. Principal component analysis showed that horizontal depth measurements, dorsal fin length and pelvic fin length were helpful in differentiating the milkfish populations. First and second principal components explained the variance of 37.62% and 31.26 % respectively. Cross-validated results of the discriminant analysis showed the overall classification rate of 90.4%. The present study decoded the differentiation and occurrence of separate stocks of milkfish in Indian waters. The findings of the present study could be utilized for conservation and management of the species

Effect of monsoon on coastal fish diversity of Goa: an example from the gillnet fishery

The dynamics of fauna in coastal ecosystems are influenced by seasonal patterns in the oceanographic environment. It is well known that the monsoon along south-west coast of India influences the movement and spawning of fishes. Thus, they have direct effect on the richness, abundance and evenness of fish diversity in the ecosystem. This study investigates how the monsoon affects the commercial gillnet fishery along the coast of Goa. Fishing experiments were conducted in popular gillnet fishing grounds and the temporal pattern in diversity indices between October 2013 and September 2014 was assessed. A total of 124 fish species (40 families), 16 crustacean species (4 families) and 9 molluscan species (8 families) were recorded. The species diversity was found to be significantly different during the monsoon season and the species abundance distribution followed a geometric series during this period indicating signs of ecosystem perturbations. The economic and biological aspects of gillnet fishing in relation to the monsoon season in Goa are also discussed

Stock identification of tiger tooth croaker Otolithes ruber (Schneider, 1801)

The tiger toothed croaker Otolithes ruber (Schneider, 1801) of the family Sciaenidae is a demersal fish that contributes significantly to the marine landings of India. To identify stock units of O. ruber based on body shape morphometry, truss network analysis was carried out based on specimens collected from four selected locations, two each from east coast (Digha and Chennai along Bay of Bengal) and the west coast (Cochin and Ratnagiri along Arabian Sea) of India. A total of 20 truss distances along the fish surface, including head, mid body and posterior region were measured and transformed measurements were subjected to factor analysis and discriminant function analysis. The bivariate plot score of factor analysis indicating two distinct stocks on both the coasts may be the consequence of geographical isolation and different environmental conditions along the Bay of Bengal (east coast) and Arabian Sea (west coast). The Ratnagiri and Cochin population showed considerable mixing which indicated the presence of a unit stock along the west coast. However, existence of strong morphometric differentiation between Digha and Chennai populations indicated two separate spawning populations in the Bay of Bengal. In the cross-validation of Discriminant function analysis, the most well classified group was Digha population (97.85%). The present study suggests the possible existence of three stocks of O. ruber, one in west coast (Cochin and Ratnagiri) and two in east coast (Digha and Chennai) which can be considered while formulating effective management strategies for the species

Ecopath modelling approach for the impact assessment of a small-scale coastal aquaculture system in Goa, India

In this study, the ecological impacts of introduction of cage aquaculture employing small cages integrating shellfish and finfish in coastal water bodies of Goa, situated in the west coast of India were analysed using Ecopath with Ecosim model. A multispecies cage aquaculture system incorporating Lutjanus argentimaculatus, Etroplus suratensis and Perna viridis was established in an estuarine ecosyste