42 research outputs found
L-Arginine promotes gut hormone release and reduces food intake in rodents
Aims: To investigate the anorectic effect of Lâarginine (LâArg) in rodents.
Methods: We investigated the effects of LâArg on food intake, and the role of the anorectic gut hormones glucagonâlike peptideâ1 (GLPâ1) and peptide YY (PYY), the Gâproteinâcoupled receptor family C group 6 member A (GPRC6A) and the vagus nerve in mediating these effects in rodents.
Results: Oral gavage of LâArg reduced food intake in rodents, and chronically reduced cumulative food intake in dietâinduced obese mice. Lack of the GPRC6A in mice and subdiaphragmatic vagal deafferentation in rats did not influence these anorectic effects. LâArg stimulated GLPâ1 and PYY release in vitro and in vivo. Pharmacological blockade of GLPâ1 and PYY receptors did not influence the anorectic effect of LâArg. LâArgâmediated PYY release modulated net ion transport across the gut mucosa. Intracerebroventricular (i.c.v.) and intraperitoneal (i.p.) administration of LâArg suppressed food intake in rats.
Conclusions: LâArg reduced food intake and stimulated gut hormone release in rodents. The anorectic effect of LâArg is unlikely to be mediated by GLPâ1 and PYY, does not require GPRC6A signalling and is not mediated via the vagus. I.c.v. and i.p. administration of LâArg suppressed food intake in rats, suggesting that LâArg may act on the brain to influence food intake. Further work is required to determine the mechanisms by which LâArg suppresses food intake and its utility in the treatment of obesity
Optimization of the reverse transcriptase polymerase chain reaction for the detection of circulating prostate cells
The reverse transcriptase polymerase chain reaction (RT-PCR) is a sensitive technique that can detect prostate-specific messenger RNA in circulating blood. Many authors have studied the potential of RT-PCR as a staging technique in prostate cancer (PC). Clinical sensitivity and in some cases specificity has been disappointing. Few authors have been able to correlate RT-PCR result with patient stage. We have compared the results of using two different RT-PCR protocols with different sensitivities on blood samples from prostate cancer patients. An 80-amplification-cycle nested primer RT-PCR assay had a detection limit of 10 prostate cells and a 50-cycle RT-PCR could detect 20 cells in 5 ml blood. The 80-cycle assay detected prostate mRNA in four of 10 female samples, whereas the 50-cycle assay detected it in none. There was little difference in the assaysâ ability to detect prostate mRNA in advanced PC patients. The 50-cycle assay could differentiate between hormone-escaped, stable hormone-treated and untreated localized PC patients, whereas the 80-cycle assay could not. Each blood sample must be assayed several times with RT-PCR to avoid false-negative results and, if this is done, assay specificity can be increased with little effect on clinical sensitivity. © 2000 Cancer Research Campaig
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A study protocol for a randomised crossover study evaluating the effect of diets differing in carbohydrate quality on ileal content and appetite regulation in healthy humans
A major component of the digesta reaching the colon from the distal ileum is carbohydrate. This carbohydrate is subject to microbial fermentation and can radically change bacterial populations in the colon and the metabolites they produce, particularly short-chain fatty acids (SCFA). However, very little is currently known about the forms and levels of carbohydrate in the ileum and the composition of the ileal microbiota in humans. Most of our current understanding of carbohydrate that is not absorbed by the small intestine comes from ileostomy models, which may not reflect the physiology of an intact gastrointestinal tract. We will investigate how ileal content changes depending on diet using a randomised crossover study in healthy humans. Participants will be inpatients at the research facility for three separate 4-day visits. During each visit, participants will consume one of three diets, which differ in carbohydrate quality: 1) low-fibre refined diet; 2) high-fibre diet with intact cellular structures; 3) high-fibre diet where the cellular structures have been disrupted (e.g. milling, blending). On day 1, a nasoenteric tube will be placed into the distal ileum and its position confirmed under fluoroscopy. Ileal samples will be collected via the nasoenteric tube and metabolically profiled, which will determine the amount and type of carbohydrate present, and the composition of the ileal microbiota will be measured. Blood samples will be collected to assess circulating hormones and metabolites. Stool samples will be collected to assess faecal microbiota composition. Subjective appetite measures will be collected using visual analogue scales. Breath hydrogen will be measured in real-time as a marker of intestinal fermentation. Finally, an continuous fermentation model will be inoculated with ileal fluid in order to understand the shift in microbial composition and SCFA produced in the colon following the different diets. ISRCTN11327221. [Abstract copyright: Copyright: © 2019 Byrne CS et al.
Period and light curve fluctuations of the Kepler Cepheid V1154 Cyg
We present a detailed period analysis of the bright Cepheid-type variable
star V1154 Cygni (V =9.1 mag, P~4.9 d) based on almost 600 days of continuous
observations by the Kepler space telescope. The data reveal significant
cycle-to-cycle fluctuations in the pulsation period, indicating that classical
Cepheids may not be as accurate astrophysical clocks as commonly believed:
regardless of the specific points used to determine the O-C values, the cycle
lengths show a scatter of 0.015-0.02 days over the 120 cycles covered by the
observations. A very slight correlation between the individual Fourier
parameters and the O-C values was found, suggesting that the O - C variations
might be due to the instability of the light curve shape. Random fluctuation
tests revealed a linear trend up to a cycle difference 15, but for long term,
the period remains around the mean value. We compare the measurements with
simulated light curves that were constructed to mimic V1154 Cyg as a perfect
pulsator modulated only by the light travel time effect caused by low-mass
companions. We show that the observed period jitter in V1154 Cyg represents a
serious limitation in the search for binary companions. While the Kepler data
are accurate enough to allow the detection of planetary bodies in close orbits
around a Cepheid, the astrophysical noise can easily hide the signal of the
light-time effect.Comment: published in MNRAS: 8 pages, 7 figure
Nova light curves from the Solar Mass Ejection Imager (SMEI) - II. The extended catalogue
We present the results from observing nine Galactic novae in eruption with the Solar Mass Ejection Imager (SMEI) between 2004 and 2009. While many of these novae reached peak magnitudes that were either at or approaching the detection limits of SMEI, we were still able to produce light curves that in many cases contained more data at and around the initial rise, peak, and decline than those found in other variable star catalogs. For each nova, we obtained a peak time, maximum magnitude, and for several an estimate of the decline time (t2). Interestingly, although of lower quality than those found in Hounsell et al. (2010a), two of the light curves may indicate the presence of a pre-maximum halt. In addition the high cadence of the SMEI instrument has allowed the detection of low amplitude variations in at least one of the nova light curves