27 research outputs found
Retalho lateral da coxa baseado na artĂ©ria genicular lateral superior: estudo anatĂ´mico, histomorfomĂ©trico e aplicações clĂnicas
Transcriptome profiling of Pinus radiata juvenile wood with contrasting stiffness identifies putative candidate genes involved in microfibril orientation and cell wall mechanics
<p>Abstract</p> <p>Background</p> <p>The mechanical properties of wood are largely determined by the orientation of cellulose microfibrils in secondary cell walls. Several genes and their allelic variants have previously been found to affect microfibril angle (MFA) and wood stiffness; however, the molecular mechanisms controlling microfibril orientation and mechanical strength are largely uncharacterised. In the present study, cDNA microarrays were used to compare gene expression in developing xylem with contrasting stiffness and MFA in juvenile <it>Pinus radiata </it>trees in order to gain further insights into the molecular mechanisms underlying microfibril orientation and cell wall mechanics.</p> <p>Results</p> <p>Juvenile radiata pine trees with higher stiffness (HS) had lower MFA in the earlywood and latewood of each ring compared to low stiffness (LS) trees. Approximately 3.4 to 14.5% out of 3, 320 xylem unigenes on cDNA microarrays were differentially regulated in juvenile wood with contrasting stiffness and MFA. Greater variation in MFA and stiffness was observed in earlywood compared to latewood, suggesting earlywood contributes most to differences in stiffness; however, 3-4 times more genes were differentially regulated in latewood than in earlywood. A total of 108 xylem unigenes were differentially regulated in juvenile wood with HS and LS in at least two seasons, including 43 unigenes with unknown functions. Many genes involved in cytoskeleton development and secondary wall formation (cellulose and lignin biosynthesis) were preferentially transcribed in wood with HS and low MFA. In contrast, several genes involved in cell division and primary wall synthesis were more abundantly transcribed in LS wood with high MFA.</p> <p>Conclusions</p> <p>Microarray expression profiles in <it>Pinus radiata </it>juvenile wood with contrasting stiffness has shed more light on the transcriptional control of microfibril orientation and the mechanical properties of wood. The identified candidate genes provide an invaluable resource for further gene function and association genetics studies aimed at deepening our understanding of cell wall biomechanics with a view to improving the mechanical properties of wood.</p
Plasticity of maritime pine (Pinus pinaster) wood-forming tissues during a growing season
Research• The seasonal effect is the most significant external source of variation affecting
vascular cambial activity and the development of newly divided cells, and hence
wood properties. Here, the effect of edapho-climatic conditions on the phenotypic
and molecular plasticity of differentiating secondary xylem during a growing season
was investigated.
• Wood-forming tissues of maritime pine (Pinus pinaster) were collected from the
beginning to the end of the growing season in 2003. Data from examination of fibre
morphology, Fourier-transform infrared spectroscopy (FTIR), analytical pyrolysis, and
gas chromatography/mass spectrometry (GC/MS) were combined to characterize the
samples. Strong variation was observed in response to changes in edapho-climatic
conditions.
• A genomic approach was used to identify genes differentially expressed during
this growing season. Out of 3512 studied genes, 19% showed a significant seasonal
effect. These genes were clustered into five distinct groups, the largest two representing
genes over-expressed in the early- or late-wood-forming tissues, respectively. The other
three clusters were characterized by responses to specific edapho-climatic conditions.
• This work provides new insights into the plasticity of the molecular machinery
involved in wood formation, and reveals candidate genes potentially responsible for
the phenotypic differences found between early- and late-wood