Enchanted Forest

https://digitalcommons.library.umaine.edu/mmb-ps/1986/thumbnail.jp

Pining For You

Trees with sailboat in ocean in backgroundhttps://scholarsjunction.msstate.edu/cht-sheet-music/12134/thumbnail.jp

Start the Day Right

Photo of Gary Nottingham; Orange backgroundhttps://scholarsjunction.msstate.edu/cht-sheet-music/10332/thumbnail.jp

Pining For You

With Ukulele arrangement. Contains advertisements and/or short musical examples of pieces being sold by publisher.https://digitalcommons.library.umaine.edu/mmb-vp/7110/thumbnail.jp

Structure–function analysis of the RNA polymerase cleft loops elucidates initial transcription, DNA unwinding and RNA displacement.

The active center clefts of RNA polymerase (RNAP) from the archaeon Pyrococcus furiosus (Pfu) and of yeast RNAP II are nearly identical, including four protruding loops, the lid, rudder, fork 1 and fork 2. Here we present a structure–function analysis of recombinant Pfu RNAP variants lacking these cleft loops, and analyze the function of each loop at different stages of the transcription cycle. All cleft loops except fork 1 were required for promoter-directed transcription and efficient elongation. Unprimed de novo transcription required fork 2, the lid was necessary for primed initial transcription. Analysis of templates containing a pre-melted bubble showed that rewinding of upstream DNA drives RNA separation from the template. During elongation, downstream DNA strand separation required template strand binding to an invariant arginine in switch 2, and apparently interaction of an invariant arginine in fork 2 with the non-template strand

Save The Last Dance For Me

Photograph of Ruth Etting; Orange, black, blue, and white waves of barshttps://scholarsjunction.msstate.edu/cht-sheet-music/12185/thumbnail.jp

Tell Me Dreamy Eyes

Woman on red background with spider webhttps://scholarsjunction.msstate.edu/cht-sheet-music/13462/thumbnail.jp

The Sweetest Girl In All The World

Illustration of a woman sitting on a chairhttps://scholarsjunction.msstate.edu/cht-sheet-music/10520/thumbnail.jp

Tomorrow / music by H. Leopold Spitalny; words by Walter Hirsch and Monte Wilhite

Cover: a photo of Gene Austin; Publisher: Forster Music Publisher (Chicago)https://egrove.olemiss.edu/sharris_d/1091/thumbnail.jp