Ion uptake and electrical potentials in plants

In the field of ion transport, animal physiologists have made substantial progress during the last decade and a great deal of information is now available concerning nerve, muscle, and various epithelial tissues. Before the techniques evolved by the animal physiologists were applied to plants a considerable delay occurred which may have been in part due to the morphological complexities of plant cells. Recently, however, these techniques have been applied with considerable success to plant cells, especially to the large internodal cells of the Characeae. It is now clear that the plant cell may be regarded as a three compartment system consisting of the cell wall, the protoplasm, and the vacuole. The cell wall acts as a physical extension of the environment but the protoplasm is bounded by the plasmalemma and tonoplast membranes which act as the diffusion barriers containing the sites at which ?active transports takes place. Electrolytes move from one compartment to another by diffusion, mass flow, solvent drag, or active transport but, in systems in which osmotic pressure is constant, diffusion and active transport are the main processes involved. It is now almost universally recognised that the electrochemical potential gradient, not simply the concentration gradient, is the driving force on an ion moving passively across a membrane, and several measurements of the driving forces on particular ions have been made. However, the forces measured are usually between the vacuole and the external medium and not the forces across the individual membranes. When the ionic fluxes have also been measured it has been possible to suggest whether active transport takes place. In a few cases, connections with metabolism have been established.Several works on the ionic relations of plants have been published recently including books by Briggs, Hope, and Robertson (1961), Sutcliffe (1962), and Jennings (1963). The general approach adopted in this thesis is that outlined in a review by Dainty (1962). It is not yet possible to treat the problem of ion transport in plants at the molecular level but, by using a suitable member of the Characeae, an attempt has been made to determine more accurately the forces acting on the ions, the membranes at which active transport takes place, and the parameters which control the electrical properties of the membranes. Electrical measurements have also been made on a higher plant organ, the exuding root system, in order to obtain evidence on the controversial problem of transport into the xylem

Large-Scale Aspects and Temporal Evolution of Pulsating Aurora

Pulsating aurora is a common phenomenon generally believed to occur mainly in the aftermath of a, substorm, where dim long-period pulsating patches appear. The study determines the temporal and spatial evolution of pulsating events using two THEN IIIS ASI stations, at Gillam (66.18 mlat, 332.78 mlon, magnetic midnight at 0634 UT) and Fort Smith, (67.38 mlat, 306.64 mlon, magnetic midnight at, 0806 UT) along roughly the same invariant latitude. Parameters have been calculated from a database of 74 pulsating aurora events from 119 days of good optical data within the period from September 2007 through March 2008 as identified with the Gillam camera. It is shown that the source region of pulsating aurora drifts or expands eastward, away from magnetic midnight, for pre-midnight onsets and that the spatial evolution is more complicated for post midnight onsets, which has implications for the source mechanism. The most probable duration of a pulsating aurora event is roughly 1.5 hours while the distribution of possible event durations includes many long (several hours) events. This may suggest that pulsating aurora is not strictly a substorm recovery phase phenomenon but rather a persistent, long-lived phenomenon that may be temporarily disrupted by auroral substorms. Observations from the Gillam station show that in fact, pulsating aurora is quite common with the occurrence rate increasing to around 60% for morning hours, with 6910 of pulsating aurora onsets occurring after substorm breakup

Persistent, Widespread Pulsating Aurora: A Case Study

Observations of a pulsating aurora event occurring on February 11, 2008, using the THEMIS all-sky imager array, indicate a spatially and temporally continuous event with a duration of greater than 15 hours and covering a region with a maximum size of greater than 9 hours MLT. The optical pulsations are at times locally interrupted or drowned out by auroral substorm activity, but are observed in the same location once the discrete aurora recedes. The pulsations following the auroral breakup appear to be brighter and have a larger patch size than pre-substorm. This suggests that, while the onset of pulsating aurora is not necessarily dependent upon a substorm precursor, the pulsations are affected and possibly enhanced by the substorm process. The long duration of such pulsating aurora events, enduring for several hours without interruption, is far longer than the expected recovery phase of a substorm, suggesting that pulsating aurora is not strictly a recovery phase phenomenon

Phase I trial combining gemcitabine and treosulfan in advanced cutaneous and uveal melanoma patients

Gemcitabine and treosulfan are DNA-damaging agents. Preclinical studies suggest that synergism exists when melanoma cells are exposed to both drugs concurrently. We conducted a phase I trial in advanced melanoma patients to determine the optimal dose of gemcitabine to be combined with treosulfan. Cohorts of three patients received increasing doses of gemcitabine, commencing at 0.5 g m−2, followed by a fixed dose of 5.0 g m−2 treosulfan on day one of a 21-day cycle. Patients alternately received a first cycle of single-agent gemcitabine or treosulfan before subsequent cycles of both drugs. Peripheral blood lymphocytes were collected in cycles 1 and 2 at various time points until 48 h post-treatment. The single-cell gel electrophoresis (Comet) assay was used to measure chemotherapy-induced DNA damage. A total of 27 patients were enrolled, no objective responses were observed, but two uveal melanoma patients had minor responses. Dose-limiting myelosuppression was reached at 3.0 g m−2 gemcitabine. DNA single-strand breaks were detected 4 h post-gemcitabine, repaired by 24 h. DNA interstrand crosslinks were detected 4 h post-treosulfan, fully removed by 48 h. Following combination chemotherapy, treosulfan-induced DNA crosslinks persisted, still being detectable 48 h post-treatment, supporting the hypothesis that gemcitabine potentiates treosulfan-induced cytotoxicity. The recommended regimen for further study is 2.5 g m−2 gemcitabine combined with 5.0 g m−2 treosulfan

Longitudinal development of a substorm brightening arc

We present simultaneous THEMIS-ground observations of longitudinal (eastward) extension of a substorm initial-brightening arc at Gillam (magnetic latitude: 65.6°) at 08:13 UT on 10 January 2008. The speed of the eastward arc extension was ~2.7 km/s. The extension took place very close to the footprints of the longitudinally separated THEMIS E and D satellites at ~12 <I>R<sub>E</sub></I>. The THEMIS satellites observed field dipolarization, weak earthward flow, and pressure increase, which propagated eastward from E to D at a speed of ~50 km/s. The THEMIS A satellite, located at 1.6 <I>R<sub>E</sub></I> earthward of THEMIS E, observed fluctuating magnetic field during and after the dipolarization. The THEMIS E/D observations suggest that the longitudinal extension of the brightening arc at substorm onset is caused by earthward flow braking processes which produce field dipolarization and pressure increase propagating in longitude in the near-earth plasma sheet

Drug resistance in cancer

Cancer Research UK has recently sponsored a meeting, organized by the UK Medical Research Council, on cancer drug resistance. Several of the molecular mechanisms responsible for this clinical outcome, such as DNA interstrand crosslink repair, apoptosis evasion, cytochrome P450 and P-glycoprotein, were discussed. There was a special focus on leukaemia, breast and ovarian cancer, and the potential use of positron-emission tomography to study anticancer-drug resistance. The progress made in translating these findings to the clinic, like Gefitinib, P-glycoprotein phenotyping, or genome-wide analysis technology, was also discussed