Use of flight interception traps of Malaise type and attractive traps for social wasps record (Vespidae: Polistinae)

The literature provides different methodologies for sampling social wasps, including, flight intercept trap type Malaise and Attractive trap, however, there is no consensus on its use. In this respect, the aim of this study was to evaluate the best use of Malaise traps and Attractive trap in biodiversity work of social wasps, and generate a collection protocol for the use of these traps. The study was conducted in the Parque Estadual do Rio Doce, located in the east of the state of Minas Gerais, in the years 2000, 2001, 2002 and 2004 and in the Botanical Garden of the Federal University of Juiz de Fora, located in the southeastern state of Minas Gerais, in years 2011, 2012 and 2013. 15 species were collected using Malaise traps, and, 26 species of social wasps were collected using Attractive traps. Although the negative aspects of both traps, complementary methodologies surveys varying social wasps are useful and it is recommended to choose for using in accordance with the logistical field

Estabilidade durante armazenamento de hambúrguer vegetal elaborado à base de caju.

bitstream/item/45550/1/BPD11003.pd

Soil water distribution for subsurface and surface drip irrigation

Devido à falta de estudos sobre o movimento da água quando aplicada abaixo da superfície, realizou-se este trabalho com o objetivo de avaliar a distribuição de água aplicada pelo sistema de gotejamento enterrado e convencional. O experimento de campo foi conduzido na área experimental do Departamento de Engenharia Rural da ESALQ/USP - Piracicaba, SP. Para o estudo, trincheiras foram abertas e instaladas sondas de TDR, dispostas a 0,05; 0,15; 0,25; 0,35 e 0,45 m profundidade, e a 0,05; 0,15; 0,25; 0,35 m comprimento, totalizando 17 sondas por trincheira. Os tratamentos foram baseados na profundidade de aplicação (0,0 e 0,10 m) e vazão aplicada (2 e 4 L h-1): ENT2; ENT4; SUP2 e SUP4. A cada hora era aplicado 1 L de água (total de 10 L), seguida de leituras com o TDR. Medições do disco úmido e saturado foram feitas com régua milimetrada; além disso, estabeleceu-se um volume controle onde foi avaliada a uniformidade de aplicação; assim, foi possível verificar, em relação aos sistemas superficiais, que os sistemas enterrados apresentaram menor área superficial molhada e atingiram maior largura e profundidade; já as maiores concentrações foram obtidas próximas ao ponto de emissão.Studies of sub-surface water movement is an interesting topic in irrigation but, in spite of the its importance, there is little literature. One of the purposes of this study was to contribute to this subject and evaluate the distribution of water applied by both subsurface drip irrigation (SDI) and conventional irrigation. Experiments were conducted at the Department of Rural Engineering (ESALQ/USP), located at Piracicaba, SP. Trenches were opened and 17 three-rod TDR probes were installed, placed at 0.05, 0.15, 0.25, 0.35 and 0.45 m depths, and to 0.05, 0.15, 0.25, 0.35 m intervals. This procedure was repeated using a dripper buried at 0 and 0.10 m for each discharge rate of 2 and 4 L h-1 tested. Wetted soil volume was observed with 1 L of water applications at intervals (total 10 L). The saturated disc around the dripper and the advance of the wetting front on the soil surface were measured with a ruler. Also, a central control volume was established inside the wetted soil volume where the uniformity of application was evaluated. The results suggest that SDI presented smaller wetted soil surface area and it reached larger distance and depth with greater storage of solution adjacent to the dripper

Leukotriene B4 mediates γδ T lymphocyte migration in response to diverse stimuli

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141926/1/jlb0323.pd

Prostaglandin E2-EP1 and EP2 receptor signaling promotes apical junctional complex disassembly of Caco-2 human colorectal cancer cells

<p>Abstract</p> <p>Background</p> <p>The apical junctional complex (AJC) is a dynamic structure responsible to maintain epithelial cell-cell adhesions and it plays important functions such as, polarity, mechanical integrity, and cell signaling. Alteration of this complex during pathological events leads to an impaired epithelial barrier by perturbation of the cell-cell adhesion system. Although clinical and experimental data indicate that prostaglandin E₂(PGE₂) plays a critical function in promoting cell motility and cancer progression, little is known concerning its role in AJC disassembly, an event that takes place at the beginning of colorectal tumorigenesis. Using Caco-2 cells, a cell line derived from human colorectal cancer, we investigated the effects of prostaglandin E₂(PGE₂) treatment on AJC assembly and function.</p> <p>Results</p> <p>Exposition of Caco-2 cells to PGE₂promoted differential alteration of AJC protein distribution, as evidenced by immunofluorescence and immunoblotting analysis and impairs the barrier function, as seen by a decrease in the transepithelial electric resistance and an increase in the permeability to ruthenium red marker. We demonstrated the involvement of EP1 and EP2 prostaglandin E₂receptor subtypes in the modulation of the AJC disassembly caused by prostanoid. Furthermore, pharmacological inhibition of protein kinase-C, but not PKA and p38MAPK significantly prevented the PGE₂effects on the AJC disassembly.</p> <p>Conclusion</p> <p>Our findings strongly suggest a central role of Prostaglandin E2-EP1 and EP2 receptor signaling to mediate AJC disassembly through a mechanism that involves PKC and claudin-1 as important target for the TJ-related effects in human colorectal cancer cells (Caco-2).</p

Oral bacterial microbiota and traumatic injuries of free-ranging Phrynops geoffroanus (Testudines, Chelidae) in southeastern Brazil

During 2006 and 2007, we collected free-ranging Phrynops geoffroanus, from two anthropogenically altered rivers in southeastern Brazil. Oral microbiological samples were taken for isolation of aerobic and facultative anaerobic bacteria; a physical examination was performed;and we evaluated possible effects on the turtles’ health. Twenty-nine species of bacteria were isolated in Piracicaba River turtles (n=10), and twenty-four species in Piracicamirim stream turtles (n=8), most of them gram-negative. In both sites, potential pathogens for reptiles were: Escherichia coli, Klebsiella pneumoniae, Enterobacter agglomerans, Citrobacter freundii, and Bacillus sp. Although boatpropeller lesions were common on the carapace of the turtles, we have not found turtles with signs of clinical diseases. The oral bacterial microbiota of P. geoffroanus inhabiting the Piracicaba River basin are composed of a diverse microbe spectrum, and long-term studies of the effects of pollution and traumatic injuries on this population and its microbial flora are warranted

PARQUE MUNICIPAL CINTURÃO VERDE DE CIANORTE – MÓDULO MANDHUY E OS PRINCIPAIS IMPACTOS DA ÁREA DE ENTORNO

O presente estudo buscou realizar uma análise geográfica a respeito das condições ambientais do Módulo Mandhuy, parte do Parque Municipal Cinturão Verde de Cianorte, localizado no Município de Cianorte-PR. Para tanto, foram investigados os processos de constituição da área de estudo, que esteve diretamente relacionado à ação de colonização da CMNP, quando realizou o planejamento do Município de Cianorte e também do seu núcleo urbano. A CMNP manteve algumas manchas de vegetação original nas proximidades e na área urbana, que mais tarde foram transformadas em áreas de preservação. Essas áreas de vegetação nativa formam quase um círculo ao redor da cidade, levando a denominação de “cinturão verde”. A metodologia utilizada para a construção desse estudo, amparou-se na busca de material bibliográfico relacionado à questão da preservação em Unidades de Conservação, sob o enfoque da Geografia Socioambiental. Em complementação a este material, foram realizadas investigações sistemáticas de campo, com auxilio de material cartográfico, buscando obter dado que pudesse identificar as pressões imprimidas a área pelos moradores das áreas vizinhas, próprias do contexto urbano

Microbiota bacteriana oral e lesões traumáticas no cágado Phrynops geoffroanus (Testudines, Chelidae) no sudeste do Brasil

Entre 2006 e 2007, coletamos amostras microbiológicas da cavidade oral de cágados Phrynops geoffroanus de vida livre, em dois rios antropizados da região sudeste do Brasil, para o isolamento de bactérias aeróbicas e anaeróbicas facultativas, e realizamos um exame físico e avaliamos possíveis efeitos sobre o estado de saúde dos animais. Vinte e nove espécies de bactérias foram isoladas em cágados do rio Piracicaba (n=10), e vinte e quatro espécies em animais do ribeirão Piracicamirim (n=8), a maioria delas gram-negativas. Em ambos os sítios estudados foram isolados patógenos potenciais para répteis, como Escherichia coli, Klebsiella pneumoniae, Enterobacter agglomerans, Citrobacter freundii e Bacillus sp. Embora lesões na carapaça dos cágados, causadas por hélices de barcos, foram um achado comum no exame físico, não encontramos animais com sinais clínicos de doenças. A microbiota bacteriana oral dos cágados que habitam a bacia do rio Piracicaba é composta por um amplo espectro de bactérias, e estudos de longa duração sobre os efeitos da poluição e de lesões traumáticas nesta população e de sua microbiota oral são recomendados.During 2006 and 2007, we collected free-ranging Phrynops geoffroanus, from two anthropogenically altered rivers in southeastern Brazil. Oral microbiological samples were taken for isolation of aerobic and facultative anaerobic bacteria; a physical examination was performed; and we evaluated possible effects on the turtles' health. Twenty-nine species of bacteria were isolated in Piracicaba River turtles (n=10), and twenty-four species in Piracicamirim stream turtles (n=8), most of them gram-negative. In both sites, potential pathogens for reptiles were: Escherichia coli, Klebsiella pneumoniae, Enterobacter agglomerans, Citrobacter freundii, and Bacillus sp. Although boat propeller lesions were common on the carapace of the turtles, we have not found turtles with signs of clinical diseases. The oral bacterial microbiota of P. geoffroanus inhabiting the Piracicaba River basin are composed of a diverse microbe spectrum, and long-term studies of the effects of pollution and traumatic injuries on this population and its microbial flora are warranted

Notes on the hematology of free-living Phrynops geoffroanus (Testudines: Chelidae) in polluted rivers of Southeastern Brazil

Phrynops geoffroanus (Schweigger, 1812) is the freshwater turtle with the widest geographical distribution in South America. During 2006, physical examination and hematological evaluation were performed on free-ranging turtles from two polluted rivers, the Piracicaba River (n = 51) and its tributary Piracicamirim (n = 42), in southeastern Brazil. Red blood cell and thrombocyte counts, mean corpuscular volume and mean corpuscular hemoglobin levels differed in turtles from the two water courses. Although free-ranging turtles showed ectoparasites and boat propeler lesions, animals apparently had no signs of clinical disease. In spite of our results, further monitoring of the demography and health status of Phrynops geoffroanus in anthropogenically altered environments is recommended.FAPESP [05/00210-9]CNPqCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES