Comparison Between the Sonic-Sys System and Inlay in II Class

In everyday dental practice we often face the problem of class II restorations. Large caries create serious problems with regard to obtaining correct contact points, marginal fit (expecially in the gingival area) and good resin polymerisation. In such cases usage of the Sonic-Sys system and inlays seems to be the best solution. The aim of this poster is to present a manual of the Sonic-Sys system, as well as comparison of the Empress II and Sonic-Sys system inlays in class II restorations. The color, marginal fit, contouring and articulating points were evaluated in clinical tests. The surfaces of the preparation were examined in SEM differences between those made by means of Sonic- Sys and standard diamond burs. The results present advantages and disadvantages of both methods. The best choice is to consider the appropriate method after clinical examination and treatment planning in each individual case

Endovenous Laser Ablation of Varicose Veins Preserves Biological Properties of Vascular Endothelium and Modulates Proinflammatory Agent Profile More Favorably Than Classic Vein Stripping

Here we compared effect of serum from varicose patients undergoing endovenous laser ablation (EVLA) and classic vein stripping (CVS) on biological properties of endothelial cells and on the local and systemic profiles of proinflammatory agents. Results showed that serum from EVLA patients improved proliferation and reduced senescence and oxidative stress in the endothelial cells, as compared with the serum from CVS patients. These effects were related to a suppressed activity of TGF-1, the level of which in the serum from the EVLA patients was decreased. Medium generated by the cells subjected to EVLA serum contained decreased amounts of ICAM-1, VCAM-1, and E-selectin and increased amount of uPA, whereas the serum itself contained decreased concentrations of ICAM-1, E-selectin, and P-selectin and increased concentrations of uPA, PAI-1, and TFPI. Both EVLA and CVS resulted in diversified patients' reaction with respect to a direction of postprocedure changes in proinflammatory factors' serum level. Analysis of proportions showed that the groups differed remarkably in case of ICAM-1 and ET-1, the level of which declined in a higher fraction of patients treated endovenously. Our findings indicate that EVLA preserves better than CVS the functionality of vascular endothelium and modulates better both local and systemic profile of proinflammatory mediators

Reporters for the analysis of N-glycosylation in Candida albicans

Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.Peer reviewedPublisher PD

Lactate signalling regulates fungal β-glucan masking and immune evasion

AJPB: This work was supported by the European Research Council (STRIFE, ERC- 2009-AdG-249793), The UK Medical Research Council (MR/M026663/1), the UK Biotechnology and Biological Research Council (BB/K017365/1), the Wellcome Trust (080088; 097377). ERB: This work was supported by the UK Biotechnology and Biological Research Council (BB/M014525/1). GMA: Supported by the CNPq-Brazil (Science without Borders fellowship 202976/2014-9). GDB: Wellcome Trust (102705). CAM: This work was supported by the UK Medical Research Council (G0400284). DMM: This work was supported by UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC/K000306/1). NARG/JW: Wellcome Trust (086827, 075470,101873) and Wellcome Trust Strategic Award in Medical Mycology and Fungal Immunology (097377). ALL: This work was supported by the MRC Centre for Medical Mycology and the University of Aberdeen (MR/N006364/1).Peer reviewedPostprin

Clinical and biological significance of RAD51 expression in breast cancer: a key DNA damage response protein

Impaired DNA damage response (DDR) may play a fundamental role in the pathogenesis of breast cancer (BC). RAD51 is a key player in DNA double-strand break repair. In this study, we aimed to assess the biological and clinical significance of RAD51 expression with relevance to different molecular classes of BC and patients’ outcome. The expression of RAD51 was assessed immunohistochemically in a well-characterised annotated series (n = 1184) of early-stage invasive BC with long-term follow-up. A subset of cases of BC from patients with known BRCA1 germline mutations was included as a control group. The results were correlated with clinicopathological and molecular parameters and patients’ outcome. RAD51 protein expression level was also assayed in a panel of cell lines using reverse phase protein array (RPPA). RAD51 was expressed in the nuclei (N) and cytoplasm (C) of malignant cells. Subcellular colocalisation phenotypes of RAD51 were significantly associated with clinicopathological features and patient outcome. Cytoplasmic expression (RAD51C+) and lack of nuclear expression (RAD51 N-) were associated with features of aggressive behaviour, including larger tumour size, high grade, lymph nodal metastasis, basal-like, and triple-negative phenotypes, together with aberrant expression of key DDR biomarkers including BRCA1. All BRCA1-mutated tumours had RAD51C+/N- phenotype. RPPA confirmed IHC results and showed differential expression of RAD51 in cell lines based on ER expression and BRCA1 status. RAD51 N+ and RAD51C+ tumours were associated with longer and shorter breast cancer-specific survival (BCSS), respectively. The RAD51 N+ was an independent predictor of longer BCSS (P<0.0001). Lack of RAD51 nuclear expression is associated with poor prognostic parameters and shorter survival in invasive BC patients. The significant associations between RAD51 subcellular localisation and clinicopathological features, molecular subtype and patients’ outcome suggest that the trafficking of DDR proteins between the nucleus and cytoplasm might play a role in the development and progression of BC

Serological Profiling of a Candida albicans Protein Microarray Reveals Permanent Host-Pathogen Interplay and Stage-Specific Responses during Candidemia

Candida albicans in the immunocompetent host is a benign member of the human microbiota. Though, when host physiology is disrupted, this commensal-host interaction can degenerate and lead to an opportunistic infection. Relatively little is known regarding the dynamics of C. albicans colonization and pathogenesis. We developed a C. albicans cell surface protein microarray to profile the immunoglobulin G response during commensal colonization and candidemia. The antibody response from the sera of patients with candidemia and our negative control groups indicate that the immunocompetent host exists in permanent host-pathogen interplay with commensal C. albicans. This report also identifies cell surface antigens that are specific to different phases (i.e. acute, early and mid convalescence) of candidemia. We identified a set of thirteen cell surface antigens capable of distinguishing acute candidemia from healthy individuals and uninfected hospital patients with commensal colonization. Interestingly, a large proportion of these cell surface antigens are involved in either oxidative stress or drug resistance. In addition, we identified 33 antigenic proteins that are enriched in convalescent sera of the candidemia patients. Intriguingly, we found within this subset an increase in antigens associated with heme-associated iron acquisition. These findings have important implications for the mechanisms of C. albicans colonization as well as the development of systemic infection

School-university partnerships for math and science education

Math and Science Programs for improving math and science education at K12 level through are analyzed in terms of a policy that establishes shool-university partnerships.M.S.Committee Member: Gordon Kingsley; Committee Member: Juan Roger

Acquisition of dynamic spatial reference by Turkish and Polish learners in French L2

Cette thèse vise à étudier comment les apprenants de deux langues sources différentes, le polonais et le turc, acquièrent la référence spatiale dynamique dans une même langue cible, le français. Nous analysons les données de production, issues de la tâche de récit de film, initiée par un film d’animation (APN, Watorek 2004), effectuées par des locuteurs natifs (groupes de contrôle des trois langues, en français, en turc et en polonais), ainsi que par les apprenants turcophones et polonophones en français langue cible. Des travaux antérieurs ont montré l’impact des langues sources sur l’acquisition de la référence spatiale dynamique en langue cible (Hickmann 2008, 2011, Demagny 2013, von Stutterheim 2003, Anastasio 2018). Compte tenu des différences typologiques entre langues à cadrage verbal dont le français et le turc et langues à satellites dont le polonais, (Talmy 2000), notre objectif est de voir dans quelle mesure ces différences impactent l’acquisition du français L2. D’une part, nos résultats issus de l’analyse des données des locuteurs natifs, révèlent une richesse et une productivité des ressources extra-verbales en turc. Ce phénomène permet de reconsidérer la place du turc au sein du groupe des langues à cadrage verbal. D’autre part, les résultats de l’analyse des productions des apprenants, ont permis de révéler des nuances qui ne se résument pas uniquement à un impact des caractéristiques typologiques des LS et ont fait également apparaitre les phénomènes acquisitionnels relevant du transfert conceptuel et de la psychotypologie des langues de Kellermann (1979).This thesis examines how learners of two different source languages, Polish and Turkish, acquire dynamic spatial reference in the target language, French. We will analyse production data from the task of describing a film storyline, based on an animated film (APN, Watorek 2004), by native speakers (control groups speaking three languages, French, Turkish and Polish), and by Turkish-speaking and Polish-speaking learners of French as a TL. Previous work has shown the impact of source languages on acquisition of dynamic spatial references in the target language (Hickmann 2008, 2011, Demagny 2013, von Stutterheim 2003, Anastasio 2018). Taking into account the typological differences between verb-framed languages including French and Turkish and satellite-framed languages including Polish (Talmy 2000), our objective is to determine the degree of impact of these differences on the acquisition of French L2. On the one hand, the results of our analysis of data from native speakers reveal a wealth and productivity of extra-verbal resources in Turkish. This phenomenon allows for reconsideration of the place of Turkish within the group of verb-framed languages. On the other hand, the results of the analysis of the learners' productions made it possible to reveal nuances that are not only limited to an impact of the typological characteristics of the SL and also revealed the acquisition phenomena relating to conceptual transfer and Kellermann's Psychotypology of Languages (1979)