Ordenha mecânica nas explorações de caprinos de Trás-os-Montes (Portugal): problemas e dificuldades para a sua divulgação

Na região de Trás-os-Montes (nordeste de Portugal) a exploração de cabras de leite, principalmente da raça Serrana, autóctone, é uma actividade relativamente importante do ponto de vista socioeconómico, porque se trata de uma região pobre e onde são raras as actividades económicas alternativas para a população rural. Para o desenvolvimento da actividade e para a melhoria da qualidade e quantidade de leite produzido, é importante melhorar as condições de ordenha, especialmente quanto às instalações e equipamentos; e às condições de trabalho do ordenhador. A maioria das explorações de caprinos da região pratica a ordenha manual. Este tipo de ordenha tem vários problemas quanto às condições de trabalho e à qualidade do leite recolhido. Nos últimos anos desenvolveram-se várias acções no sentido de melhorar as condições de ordenha e de incentivar os criadores para a adopção de ordenha mecânica. No entanto, verificam-se alguns problemas e dificuldades dos criadores na adopção e utilização destes equipamentos. Com este trabalho, pretende-se identificar os problemas e dificuldades que resultaram da adopção/utilização de equipamentos de ordenha mecânica em explorações de cabras de leite da região de Trás-os-Montes. O estudo da ordenha mecânica abrangeu vários aspectos: os equipamentos utilizados; a organização do trabalho e das instalações; e os procedimentos do ordenhador no trabalho de ordenha. Foram estudadas as explorações de caprinos de raça Serrana da região de Trás-os-Montes que utilizam qualquer tipo de ordenha mecânica. A informação recolhida permite concluir que há dificuldades na adaptação ao uso de novas técnicas de ordenha e equipamentos. Os problemas estão, principalmente, relacionados com a organização do trabalho e do local da ordenha, e com a aprendizagem da correcta utilização dos equipamentos. De futuro, será importante considerar o desenvolvimento de acções de apoio técnico aos criadores, no sentido de melhorar as instalações/local de ordenha e a utilização dos equipamentosIn the national context, the region of Trás-os-Montes, in Northeastern Portugal, is one of the most depopulated and least favoured areas. In this region, goat breeding has great socioeconomic importance due to the number of families that are economically dependent on these activities. This importance is particularly due to the scarcity of alternative economic activities. To improve goat breeding, milk production and milk quality, it is important to enhance milking conditions, mainly in facilities, equipment and working conditions. Presently, there are very few farms with milking machines. Mostly, goats are hand-milked inside the shelters. As a result of actions carried out over the recent years involving strategies to improve milking conditions on goat farms in the region, some breeders adopted mechanical milking. For these breeders, mechanical milking is a new technology, they are inexperienced and they lack knowledge about the correct use of these machines and best milking practices. To study mechanical milking we established the following aspects to consider: working organization and facilities, milking operations, and procedures performed by milkers. Goat farms that used mechanical milking in the region of Trás-os-Montes were studied. From the information collected, it was found some difficulties in the adaptation to the use of new milking techniques. Main problems are related to working organization, facilities and equipment utilization

Chemical, cytotoxic, and anti-inflammatory assessment of honey bee venom from Apis mellifera intermissa

The venom from Apis mellifera intermissa, the main honey bee prevailing in Morocco, has been scarcely studied, despite its known potential for pharmacological applications. In the present work, we investigated the composition, the anti-inflammatory activity, and the venom’s cytotoxic properties from fifteen honey bee venom (HBV) samples collected in three regions: northeast, central, and southern Morocco. The chemical assessment of honey bee venom was performed using LC-DAD/ESI/MSn , NIR spectroscopy and AAS spectroscopy. The antiproliferative effect was evaluated using human tumor cell lines, including breast adenocarcinoma, non-small cell lung carcinoma, cervical carcinoma, hepatocellular carcinoma, and malignant melanoma. Likewise, we assessed the anti-inflammatory activity using the murine macrophage cell line. The study provides information on the honey bee venom subspecies’ main components, such as melittin, apamin, and phospholipase A2, with compositional variation depending on the region of collection. Contents of toxic elements such as cadmium, chromium, and plumb were detected at a concentration below 5 ppm, which can be regarded as safe for pharmaceutical use. The data presented contribute to the first study in HBV from Apis mellifera intermissa and highlight the remarkable antiproliferative and anti-inflammatory effects of HBV, suggesting it to be a candidate natural medicine to explore.info:eu-repo/semantics/publishedVersio

Identificando genes que favorecen la adaptación local en Apis mellifera iberiensis

En especies con distribución amplia, las poblaciones locales experimentan frecuentemente condiciones ambientales heterogéneas, bajo las que evolucionan a lo largo de los años. En tales casos, estas condiciones ambientales son supuestamente responsables de moldear la distinta composición genética entre las poblaciones. Sin embargo, la adaptación local puede variar dependiendo de la naturaleza de la adaptación, la fuerza de la selección, el flujo génico, el tamaño efectivo de la población, el nivel de aislamiento genético y la complejidad de los caracteres bajo selección. Para entender mejor estos procesos evolutivos, se ha analizado el genoma de la abeja ibérica, A. m. iberiensis, en búsqueda de señales de selección en aquellos genes que favorecen su adaptación local usando los polimorfismos de nucleótidos simples (SNPs) coma marcadores. Con este fin, se colectaron un total de 711 individuos de A. m. iberiensis en 23 sítios a lo largo de tres transectos Norte-Sur en la Peninsula lbérica. Esta colección fue genotipada con 1536 SNPs. Se usaron cinco métodos para buscar señales de selección: LOSITAN, ARLEQUIN, BAYESFST, BAYESCAN y MatSAM. Estos métodos identificaron en total 74 SNPs con señales de selección, de los cuales 10 SNPs exhibieron una señal de selección muy fuerte. Los SNPs que presentaron señales de selección fueron localizados en el mapa genómico de A. mellifera y se anotó su relación con determinados genes o posibles genes. Las funciones potenciales de estos genes se identificaron usando varias bases de datos genómicas. Los 10 SNPs con señal de selección mas fuerte destacaron por estar ligados a posibles genes con funciones biológicas relacionadas con la visión, la detoxificación de xenobióticos y la inmunidad.info:eu-repo/semantics/publishedVersio

Development of a UPLC-MS/MS method for establishing Serra da Estrela´s free amino acids profile

Associate Laboratory LSRE-LCM-UID/EQU/50020/2019,strategic funding UID/BIO/04469/2019-CEB and BioTecNorte operation (NORTE-01-0145-FEDER-000004), strategic project PEst-OE/AGR/UI0690/2014–CIMOand by Project 02/SAICT/ 2016/23290-QCLASSE, funded by national funds through FCT/MCTES (PIDDAC)info:eu-repo/semantics/publishedVersio

Chemical, Cytotoxic, and Anti-Inflammatory Assessment of Honey Bee Venom from Apis mellifera intermissa

The venom from Apis mellifera intermissa, the main honey bee prevailing in Morocco, has been scarcely studied, despite its known potential for pharmacological applications. In the present work, we investigated the composition, the anti-inflammatory activity, and the venom’s cytotoxic properties from fifteen honey bee venom (HBV) samples collected in three regions: northeast, central, and southern Morocco. The chemical assessment of honey bee venom was performed using LC-DAD/ESI/MSn, NIR spectroscopy and AAS spectroscopy. The antiproliferative effect was evaluated using human tumor cell lines, including breast adenocarcinoma, non-small cell lung carcinoma, cervical carcinoma, hepatocellular carcinoma, and malignant melanoma. Likewise, we assessed the anti-inflammatory activity using the murine macrophage cell line. The study provides information on the honey bee venom subspecies’ main components, such as melittin, apamin, and phospholipase A2, with compositional variation depending on the region of collection. Contents of toxic elements such as cadmium, chromium, and plumb were detected at a concentration below 5 ppm, which can be regarded as safe for pharmaceutical use. The data presented contribute to the first study in HBV from Apis mellifera intermissa and highlight the remarkable antiproliferative and anti-inflammatory effects of HBV, suggesting it to be a candidate natural medicine to exploreinfo:eu-repo/semantics/publishedVersio

Production of chitosan-based biodegradable active films using bio-waste enriched with polyphenol propolis extract envisaging food packaging applications

Developing biodegradable active films has been a promising green approach to overcoming global concerns over the environmental pollution and human health caused by plastic utilization. This study aimed to develop active films based on chitosan (CS), produced from waste crayfish (Procambarus clarkii) shells enriched with bioactive extract (5–20%) of propolis (PS) and to characterize its properties, envisaging food packaging applications. The chromatographic profile of PS extract confirmed its richness, with 41 phenolic compounds. With increasing extract addition to the chitosan, the thickness of the films increased from 61.7 to 71.7 μm, causing a reduction in the light transmission rate, along with a greenish colour shift. The interactions between PS extract and CS was confirmed by infrared spectroscopy, at the same time that the microstructural integrity of the films was checked on the scanning electron microscopy micrographs. The findings also showed that addition of PS enhanced the films thermal stability and mechanical properties e.g., tensile modulus, yield strength, and stress at break. Besides, it improved the antioxidant and antimicrobial activities. Overall, CS-based composite films seem a promising green alternative to petroleum-based synthetic plastics allowing to extend the shelf life of food products due to their eco-friendly nature.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020) and contracts through the individual and institutional scientific employment program-contract with Soraia I. Falcão and Arantzazu Santamaria Echart. Thanks to the Programa Apícola Nacional 2020-2022 (National Beekeeping Program) for funding the project “Standardization of production procedures and quality parameters of bee products” and to Project PDR2020-1.0.1- FEADER-031734: “DivInA-Diversification and Innovation on Beekeeping Production”. Finally, this work is funded by the European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project GreenHealth - Digital strategies in biological assets to improve well-being and promote green health, Norte-01-0145-FEDER-000042.info:eu-repo/semantics/publishedVersio

Deontologia, ética e valores na educação: revisitar a história da educação: professora Cândida Florinda Ferreira

A deontologia, a ética e os Valores na Educação, são hoje um motivo de preocupação e de profunda reflexão de toda a comunidade educativa. Numa época em que o acesso à formação contínua é facilitada, devemos revisitar autores e pedagogos da nossa história da Educação mantendo um diálogo constante com o passado para melhor entender esta temática actual, quer através da visita às políticas educativas legisladas e praticadas, quer no diálogo com os pedagogos e agentes da educação

Thermal Stability and Antioxidant Activity of Bioactive Compounds in Bread Enriched with Bee Pollen and Bee Bread

Bee pollen (BP) and bee bread (BB) are natural food sources containing a wide variety of bioactive compounds, complementing their rich nutritional composition. These bee products are being explored to empower functional foods, with the term functionality being dependent on the bioactive compounds added to the food matrix. However, there is not enough evidence of the effect of heat on these compounds during food processing and production and how it impacts their biological activity. Here, we enriched traditional bread by adding BP and BB at different proportions of 1 to 5% and tested the thermal stability of their bioactive compounds through several spectroscopic and chromatographic analyses. Adding bee pollen and bee bread to bread resulted in a 4 and 5-fold increase in total phenolic content, respectively. While not all the 38 phenolic and phenolamide compounds identified in the raw BP and BB were detected in the processed bread, phenolamides were found to be more resilient to baking and heat treatment than flavonoids. Still, the enriched bread’s antioxidant activity improved with the addition of BP and BB. Therefore, incorporating bee products into heat-treated products could enhance the functionality of staple foods and increase the accessibility to these natural products

Improvement of the In Vitro Cytotoxic Effect on HT-29 Colon Cancer Cells by Combining 5-Fluorouacil and Fluphenazine with Green, Red or Brown Propolis

Cancer is regard as one of the key factors of mortality and morbidity in the world. Treatment is mainly based on chemotherapeutic drugs that, when used in targeted therapies, have serious side effects. 5-fluorouracil (5-FU) is a drug commonly used against colorectal cancer (CRC), despite its side effects. Combination of this compound with natural products is a promising source in cancer treatment research. In recent years, propolis has become the subject of intense pharmacological and chemical studies linked to its diverse biological properties. With a complex composition rich in phenolic compounds, propolis is described as showing positive or synergistic interactions with several chemotherapeutic drugs. The present work evaluated the in vitro cytotoxic activity of the most representative propolis types, such as green, red and brown propolis, in combination with chemotherapeutic or CNS drugs on HT-29 colon cancer cell lines. The phenolic composition of the propolis samples was evaluated by LC-DAD-ESI/MSn analysis. According to the type of propolis, the composition varied; green propolis was rich in terpenic phenolic acids and red propolis in polyprenylated benzophenones and isoflavonoids, while brown propolis was composed mainly of flavonoids and phenylpropanoids. Generally, for all propolis types, the results demonstrated that combing propolis with 5-FU and fluphenazine successfully enhances the in vitro cytotoxic activity. For green propolis, the combination demonstrated an enhancement of the in vitro cytotoxic effect compared to green propolis alone, at all concentrations, while for brown propolis, the combination in the concentration of 100 μg/mL gave a lower number of viable cells, even when compared with 5-FU or fluphenazine alone. The same was observed for the red propolis combination, but with a higher reduction in cell viability. The combination index, calculated based on the Chou–Talalay method, suggested that the combination of 5-FU and propolis extracts had a synergic growth inhibitory effect in HT-29 cells, while with fluphenazine, only green and red propolis, at a concentration of 100 μg/mL, presented synergism

Long-term response and design of two geosynthetics: effect of field installation damage

This paper contributes to understanding how installation damage of geosynthetics influences their long-term response and design. A geotextile and a geogrid were exhumed after installation under real conditions; their long-term tensile behaviour was investigated using conventional creep and creep rupture tests. Reduction factors for installation damage, creep and their combined effect were computed. The main aim of the paper was to assess how the long-term response of the geosynthetics was influenced by installation damage and whether it would be necessary to update current design approaches. The installation damage affected the mechanical response of both geosynthetics; important strength reductions were observed, particularly for the most severe installation conditions. The results indicate that, contrary to what has been reported in the literature, the creep rupture response of the geotextile changed after installation damage. When tested under similar creep loads (fraction of the sample tensile strength), both geosynthetics exhibited reduced potential for creep rupture and smaller strains at the end of primary creep. Changes in stiffness were less important than those for strength, for both short- and long-term response. There was synergy between installation damage and creep; the traditional approach to design was unsafe for the geotextile and slightly conservative for the geogrid.info:eu-repo/semantics/publishedVersio