From Sandstone to Grindstone

From Sandstone to Grindstone investigates the environment in which my father became the sort of man who would literally work himself to death to support the people he loved. While the characters are fictional, their adventures are all in some way inspired by the stories he used to tell of his childhood in West Virginia, and the protagonist, Johnny, is named after my father and embodies all of the characteristics I believe he would have had in his formative years. While there is no single definitive answer to why he worked so hard, the one theme that each story seems to come back to is that of accountability—living with the consequences of one’s actions. In each story, one or more of the characters is faced with a life-changing decision and each character deals with the emotional aftermath in a different way. Some characters respond in overtly negative ways, by behaving recklessly or even losing their grip on reality. But for Johnny, those emotions are concentrated into an irrepressible drive to do right by the people he loves, even if it means sacrificing himself

Varying Feedback Strategy and Scheduling in Simulator Training: Effects on Learner Perceptions, Initial Learning, and Transfer

This experimental study investigated the effects of visual feedback on initial learning, perceived self-efficacy, workload, near transfer, far transfer, and perceived realism during a simulator-based training task. Prior studies indicate that providing feedback is critical for schema development (Salmoni, Schmidt, & Walter 1984; Sterman, 1994). However, its influence has been shown to dissipate and is not directly proportionate to the frequency at which it is given (Wulf, Shea, & Matschiner, 1998). A total of 54 participants completed the study forming six treatment groups. The independent treatment, visual feedback, was manipulated as scheduling (absolute—every practice trial or relative—every third trial) and strategies (gradual decrease of visual cues within the interface, gradual increase of visual cues within the interface, or a single consistent cue for each trial). Participants completed twelve practice trials of welding under one of six feedback manipulations; then, participants completed twelve practice trials of welding without it. Lastly, participants performed the weld task on actual equipment in a shop area. No treatment showed significant difference among groups with regard to initial learning, retention, near transfer, and far transfer measures. However, a statistical significance was found during initial learning and retention within each treatment group. Findings support empirical evidence that a variability of practice paradigm promotes learning (Lee & Carnahan, 1990; Shea & Morgan, 1979). Learner perceptions of realism suggest that novice learners perceive simulator fidelity as high, however, these perceptions may dissipate as the learner practices. Those groups that involved the greatest number of cues at the onset of practice or having cues available at every other trial reported the greatest amount of workload. All groups reported increases in perceptions of self-efficacy during practice on the simulator, but those perceptions decreased when participants performed the weld task on actual equipment. Findings suggest that contextual-interference of increasing, decreasing, or changing feedback counteracts the guidance effect of feedback as found in previous studies

The Promise of Comprehensive Community Development: Ten Years of Chicago's New Communities Program

Developed by the Local Initiatives Support Corporation of Chicago (LISC Chicago) as a way of promoting comprehensive community development among local groups, NCP is one of the largest single-city efforts to help develop local capacity, partnerships, and resources. (Box ES.1 defines the key terms associated with these efforts.) Although NCP continues in Chicago, this report is focused on the first 10 years of MacArthur's support for the program. This fourth and final report on the first 10 years of NCP describes the guiding principles behind the initiative, the extent to which it achieved its goals, the challenges it faced, and the implications of these challenges and successes for similar initiatives. As a flagship example of a significant comprehensive community initiative (CCI) -- and one that has served as a model for recent federal policy -- the NCP experience offers important lessons for the next generation of placebased CCIs

Tectonics of Atlantic Canada

The tectonic history of Atlantic Canada is summarized according to a model of multiple ocean opening-closing cycles. The modern North Atlantic Ocean is in the opening phase of its cycle. It was preceded by an early Paleozoic lapetus Ocean whose cycle led to formation of the Appalachian Orogen. lapetus was preceded by the Neoproterozoic Uranus Ocean whose cycle led to formation of the Grenville Orogen. The phenomenon of coincident, or almost coincident orogens and modern continental margins that relate to repeated ocean opening-closing cycles is called the Accordion Effect. An understanding of the North Atlantic Ocean and its continental margins provides insights into the nature of lapetus and the evolution of the Appalachian Orogen. Likewise, an understanding of lapetus and the Appalachian Orogen raises questions about Uranus and the development of the Grenville Orogen. Modern tectonic patterns in the North Atlantic may have been determined by events that began before 1000 m.y. Résumé L'histoire tectonique de la portion atlantique du Canada est présenté comme la résultante d'une série d'ouvertures et de fermetures océaniques. Selon ce modèle tectonique, l'Atlantique nord moderne serait actuellement dans sa phase d'ouverture. Au début du Paléozoïque, le cycle précédent de l'océan lapétus a engendré l'orogène des Appalaches. L'océan lapétus a été précédé au Néoprotérozoïque par l'océan Uranus, dont le cycle d'ouverture-fermeture a engendré l'orogène de Grenville. Le phénomène de coïncidence ou quasi-coïncidence du profil des diverses orogènes et des marges continentale modernes qui correspond aux multiples cycles d'ouvertures-fermetures se nomme l'effet accordéon. La connaissance de l'océan Atlantique nord et de ses marges continentales permet d'appréhender certaines caractéristiques de la nature de l'océan lapétus et de l'orogène appalachîen. De même, une connaissance de l'océan lapétus et de l'orogène appalachîen suscite des pistes de questionnement sur l'océan Uranus et l'orogène de Grenville. Les profils de l'océan Atlantique nord actuelle pourrait bien être le résultat d'événements qui auraient débuté il y a environ 1 000 Ma

School playgrounds and physical activity policies as predictors of school and home time activity

<p>Abstract</p> <p>Background</p> <p>Previous work has suggested that the number of permanent play facilities in school playgrounds and school-based policies on physical activity can influence physical activity in children. However, few comparable studies have used objective measures of physical activity or have had little adjustment for multiple confounders.</p> <p>Methods</p> <p>Physical activity was measured by accelerometry over 5 recess periods and 3 full school days in 441 children from 16 primary schools in Dunedin, New Zealand. The number of permanent play facilities (swing, fort, slide, obstacle course, climbing wall etc) in each school playground was counted on three occasions by three researchers following a standardized protocol. Information on school policies pertaining to physical activity and participation in organized sport was collected by questionnaire.</p> <p>Results</p> <p>Measurement of school playgrounds proved to be reliable (ICC 0.89) and consistent over time. Boys were significantly more active than girls (P < 0.001), but little time overall was spent in moderate-vigorous physical activity (MVPA). Boys engaged in MVPA for 32 (SD 17) minutes each day of which 17 (10) took place at school compared with 23 (14) and 11 (7) minutes respectively in girls. Each additional 10-unit increase in play facilities was associated with 3.2% (95% CI 0.0-6.4%) more total activity and 8.3% (0.8-16.3%) more MVPA during recess. By contrast, school policy score was not associated with physical activity in children.</p> <p>Conclusion</p> <p>The number of permanent play facilities in school playgrounds is associated with higher physical activity in children, whereas no relationship was observed for school policies relating to physical activity. Increasing the number of permanent play facilities may offer a cost-effective long-term approach to increasing activity levels in children.</p

Identification of Select Fumonisin Forming Fusarium Species Using PCR Applications of the Polyketide Synthase Gene and its Relationship to Fumonisin Production in vitro

A polymerase chain reaction (PCR) based diagnostic assay was used to develop markers for detection of Fusarium verticillioides (=F. moniliforme), a fumonisin producing fungus in maize tissues. Species-specific primers were designed based on sequence data from the polyketide synthase (PKS) gene (FUM1- previously FUM5) responsible for fumonisin production in fungi. Four sets of oligonucleotide primers were tested for their specificity using 24 strains of F. verticillioides, 10 F. proliferatum, and 12 of other Fusarium species. In addition, 13 species of other fungal genera, from four phyla, were tested as negative controls. Among the four sets, primer set B consistently amplified a 419-bp fragment from the DNA 96% of all F. verticillioides strains and 83% of F. proliferatum. All other fungi tested were negative using primer set B. A total of 38% of the F. verticillioides strains grown on a selective liquid medium produced fumonisin and 92% formed the toxin on standard rice medium. When fumonisin formed in culture, PCR assay using primer set B detected every strain of F. verticillioides, but only amplified 80% of F. proliferatum strains that produced the toxin. PCR detection was consistent at 100 pg/μl concentration of genomic DNA from 4 F. verticillioides strains, but varied at 10 pg/μl. Two duplicate greenhouse tests using artificially inoculated maize plants, had greater levels of F. verticillioides detected after re-evaluting using primer set B than from culturing of the tissues. The molecular protocols described in this study requires only 1 day for completion compared to approximately 10 days for cultural work and morphological determination. In conclusion, conventional PCR assay using primer set B provides a sensitive and accurate detection assay that can be used as a primary or secondary confirmation method for identification and occurrence of F. verticillioides within the maize tissues. However, studies using primer set B for fumonisin production determined by strains of F. verticillioides and F. proliferatum will require further verification

U.S. adolescent and adult women\u27s experiences accessing and using toilets in schools, workplaces, and public spaces: A multi-site focus group study to inform future research in bladder health

The World Health Organization recognizes access to clean and safe toilets as crucial for public health. This study explored U.S. adolescent and adult cisgender women\u27s lived experiences accessing toilets in schools, workplaces, and public spaces. As part of the Prevention of Lower Urinary Tract Symptoms (PLUS) Research Consortium, we conducted 44 focus groups with female participants (n = 360; ages 11-93). Focus groups were stratified by age (11-14, 15-17, 18-25, 26-44, 45-64, 65+) and conducted across 7 geographically diverse U.S. sites from July 2017-April 2018. Using a transdisciplinary approach, we conducted conventional qualitative coding informed by our PLUS conceptual framework and used content analysis processes to identify salient themes. Across settings, toilet access was restricted by gatekeepers (i.e., individuals who control access to toilets). In contrast, self-restricting toilet use (deciding not to use the toilet despite biologic need to urinate) was based on internalized norms to prioritize school and job responsibilities over urination. In public spaces, self-restricting use was largely in response to lack of cleanliness. Across the life course, participants perceived gender disparities in the ability to easily access public toilets. Further research is needed to determine if and how these factors impact bladder health across the life course

Gender Differences in Coping with Racism: African American Experience and Empowerment

Black men and women encounter multiple forms of racism in American society and require numerous strategies to manage the stress associated with these experiences. This chapter reviews the current state of the literature regarding Black people and how they cope with racism. Findings demonstrate that Black people tend to cope with racism through social support, religion, avoidance, and problem-focused coping, with some gender differences in coping approaches. We also contrast functional versus dysfunctional coping approaches and underscore the importance of empowerment to promote well-being and social change. Limitations of this review include the predominance of American-based samples used in the literature, which often excludes other Black ethnic and national groups. Further, the experiences of other Black intersectional identities are not well represented in the literature and require more study as their experiences of coping with racism may differ

Enhancing DNA electrotransformation efficiency in Escherichia coli DH10B electrocompetent cells

Electrotransformation also known as electroporation is the most reliable and efficient tool for plasmid DNA uptake. Electrotransformation efficiency is function of many factors which include (1) number of cell washes prior to electroporation, (2) electroporation cell number, (3) electroporation DNA amount, and (4) cell growth phase. Those factors have limitedly been concomitantly investigated in E. coli DH10B strain. This study is aimed to explore above key factors to define the optimal conditions for high electrotransformation efficiency. The results showed that electrotransformation efficiency of E. coli DH10B was enhanced to 1.5 x 10(9) cfu/µg by washing cells three times with 15 ml of 10% glycerol. This washed off extra salts from cell suspension and enhanced electrotransformation by preventing arcing and enhancing cell resistance while ensuring minimal level of conductivity. Early exponential phase at 0.15 OD600 was the best growth phase for enhancing electrotransformation of E. coli DH10B. The results also showed that higher electrotransformation efficiency was similarly achieved when 0.5 x 10(10) and 0.6 x 10(10) cell numbers were electroporated with DNA amount ranging from 10 to 40 pg. This study confirmed the optimal conditions for electro competent cell preparation and plasmid DNA electrotransformation, which can result highest transformation efficiency