How Technologies of Serial Visualization, Arrangement, and Narration Stabilized Eoliths as Archeological Objects

Dieser Beitrag setzt sich mit den historischen Kontroversen um die sogenannten Eolithen auseinander: Waren diese sehr rudimentär abgeschlagenen Steine aus europäischen Tertiärschichten tatsächlich das Resultat menschlicher Arbeit? Der Fokus ist auf die narrativen, visuellen und räumlichen Argumente einiger Eolithen-Verfechter gerichtet. Eine wirkmächtige Strategie war die Integration der vermeintlichen Werkzeuge in geologische, archäologische und paläoanthropologische Serien, um damit an etabliertes Wissen und an die kulturelle Bedeutung des Seriellen anzuschließen. Zuvor mussten die Feuersteine jedoch in Transkriptionskaskaden von Objekten in situ in Zeichnungen und serielle Abbildungen in Publikationen übersetzt werden, um schließlich den Abstraktionsgrad von Elementen in hoch formalisierten Tabellen einander gegenübergestellter Serien zu erreichen. In meiner Diskussion nehme ich diese Aspekte der Wissensgenerierung im Transit zwischen unterschiedlichen Gemeinschaften, Räumen und Medien ins Visier

History in the Gene: Negotiations Between Molecular and Organismal Anthropology

In the advertising discourse of human genetic database projects, of genetic ancestry tracing companies, and in popular books on anthropological genetics, what I refer to as the anthropological gene and genome appear as documents of human history, by far surpassing the written record and oral history in scope and accuracy as archives of our past. How did macromolecules become "documents of human evolutionary history”? Historically, molecular anthropology, a term introduced by Emile Zuckerkandl in 1962 to characterize the study of primate phylogeny and human evolution on the molecular level, asserted its claim to the privilege of interpretation regarding hominoid, hominid, and human phylogeny and evolution vis-à-vis other historical sciences such as evolutionary biology, physical anthropology, and paleoanthropology. This process will be discussed on the basis of three key conferences on primate classification and evolution that brought together exponents of the respective fields and that were held in approximately ten-years intervals between the early 1960s and the 1980s. I show how the anthropological gene and genome gained their status as the most fundamental, clean, and direct records of historical information, and how the prioritizing of these epistemic objects was part of a complex involving the objectivity of numbers, logic, and mathematics, the objectivity of machines and instruments, and the objectivity seen to reside in the epistemic objects themselve

Becoming a Viking: DNA testing, genetic ancestry and placeholder identity

A consensus has developed among social and biological scientists around the problematic nature of genetic ancestry testing, specifically that its popularity will lead to greater genetic essentialism in social identities. Many of these arguments assume a relatively uncritical engagement with DNA, under ‘highstakes’ conditions. We suggest that in a biosocial society, more pervasive ‘lowstakes’ engagement is more likely. Through qualitative interviews with participants in a study of the genetic legacy of the Vikings in Northern England, we investigate how genetic ancestry results are discursively worked through. The identities formed in ‘becoming a Viking’ through DNA are characterized by fluidity and reflexivity, rather than essentialism. DNA results are woven into a wider narrative of selfhood relating to the past, the value of which lies in its potential to be passed on within families. While not unproblematic, the relatively banal nature of such narratives within contemporary society is characteristic of the ‘biosociable’

新機能性物質設計の基礎的研究 : 熱力学量と構造の関係について

博士（理学）神戸大

Patient-reported outcomes in patients with hematological relapse or progressive disease:a longitudinal observational study

Abstract Background Patients with hematological cancer who experience relapse or progressive disease often face yet another line of treatment and continued mortality risk that could increase their physical and emotional trauma and worsen their health-related quality of life. Healthcare professionals who use patient-reported outcomes to identify who will have specific sensitivities in particular health-related quality of life domains may be able to individualize and target treatment and supportive care, both features of precision medicine. Here, in a cohort of patients with relapsed or progressive hematological cancer, we sought to identify health-related quality of life domains in which they experienced deterioration after relapse treatment and to investigate health-related quality of life patterns. Method Patients were recruited in connection with a precision medicine study at the Department of Hematology, Aalborg University Hospital. They completed the European Organization for Research and Treatment of Cancer questionnaire and the Hospital Anxiety and Depression Scale at baseline and at 3, 6, 9, and 12 months after the relapse diagnosis or progressive cancer. Modes of completion were electronically or on paper. Clinically relevant changes from baseline to 12 months were interpreted according to Cocks’ guidelines. We quantified the number of patients with moderate or severe symptoms and functional problems and the number who experienced improvements or deterioration from baseline to 12 months. Results A total of 104 patients were included, of whom 90 (87%) completed baseline questionnaires and 50 (56%) completed the 12-month assessments. The three symptoms that patients most often reported as deteriorating were fatigue (18%), insomnia (18%), and diarrhea (18%). The three functions that patients most often reported as deteriorating were role (16%) and emotional (16%) and cognitive (16%) functioning. Conclusion In this study, patient-reported outcome data were useful for identifying negatively affected health-related quality of life domains in patients with relapsed or progressive hematological cancer. We identified patients experiencing deterioration in health-related quality of life during treatment and characterized a potential role for patient-reported outcomes in precision medicine to target treatment and supportive care in this patient group

<資料>官房學と比較經營學

Spliceosome mutations are frequently observed in patients with myelodysplastic syndromes (MDS). However, it is largely unknown how these mutations contribute to the disease. MicroRNAs (miRNAs) are small noncoding RNAs, which have been implicated in most human cancers due to their role in post transcriptional gene regulation. The aim of this study was to analyze the impact of spliceosome mutations on the expression of miRNAs in a cohort of 34 MDS patients. In total, the expression of 76 miRNAs, including mirtrons and splice site overlapping miRNAs, was accurately quantified using reverse transcriptase quantitative PCR. The majority of the studied miRNAs have previously been implicated in MDS. Stably expressed miRNA genes for normalization of the data were identified using GeNorm and NormFinder algorithms. High-resolution melting assays covering all mutational hotspots within SF3B1, SRSF2, and U2AF1 (U2AF35) were developed, and all detected mutations were confirmed by Sanger sequencing. Overall, canonical miRNAs were downregulated in spliceosome mutated samples compared to wild-type (P = 0.002), and samples from spliceosome mutated patients clustered together in hierarchical cluster analyses. Among the most downregulated miRNAs were several tumor-suppressor miRNAs, including several let-7 family members, miR-423, and miR-103a. Finally, we observed that the predicted targets of the most downregulated miRNAs were involved in apoptosis, hematopoiesis, and acute myeloid leukemia among other cancer- and metabolic pathways. Our data indicate that spliceosome mutations may play an important role in MDS pathophysiology by affecting the expression of tumor suppressor miRNA genes involved in the development and progression of MDS

Development of a Precision Medicine Workflow in Hematological Cancers, Aalborg University Hospital, Denmark

Within recent years, many precision cancer medicine initiatives have been developed. Most of these have focused on solid cancers, while the potential of precision medicine for patients with hematological malignancies, especially in the relapse situation, are less elucidated. Here, we present a demographic unbiased and observational prospective study at Aalborg University Hospital Denmark, referral site for 10% of the Danish population. We developed a hematological precision medicine workflow based on sequencing analysis of whole exome tumor DNA and RNA. All steps involved are outlined in detail, illustrating how the developed workflow can provide relevant molecular information to multidisciplinary teams. A group of 174 hematological patients with progressive disease or relapse was included in a non-interventional and population-based study, of which 92 patient samples were sequenced. Based on analysis of small nucleotide variants, copy number variants, and fusion transcripts, we found variants with potential and strong clinical relevance in 62% and 9.5% of the patients, respectively. The most frequently mutated genes in individual disease entities were in concordance with previous studies. We did not find tumor mutational burden or micro satellite instability to be informative in our hematologic patient cohort