Maximally local two-nucleon interactions at fourth order in delta-less chiral effective field theory

We present new maximally-local two-nucleon interactions derived in delta-less chiral effective field theory up to next-to-next-to-next-to leading order that include all contact and pion-exchange contributions to the nuclear Hamiltonian up to this order. Our interactions are fit to nucleon-nucleon phase shifts using a Bayesian statistical approach, and explore a wide cutoff range from $0.6-0.9$ fm ($\sim$ 440 MeV to $\sim$ 660 MeV). These interactions can be straightforwardly employed in accurate quantum Monte Carlo methods, such as the auxiliary field diffusion Monte Carlo method. Together with local three-nucleon forces, calculations with these new interactions will provide improved benchmarks for the structure of atomic nuclei and serve as crucial input to analyses of exciting astrophysical phenomena involving neutron stars, such as binary neutron-star mergers.Comment: 19 pages, 8 figures. Comments welcom

Characterising the traditional organic liquid formulations used by the farmers of western agro climatic zone of Tamil Nadu

The study was conducted to document the preparation method of organic liquid formulations used by the farmers belongs to the western agro climatic zone of Tamil Nadu and to characterise the same. Most of the formulations have been used as manure, pest and disease control agents since ancient times. In this study, for the first time, we attempted to characterise all the organic liquid formulations by measuring its physiochemical properties (pH, EC, OC, TDS), macronutrients (N, P, K) and micronutrients (Zn, Cu, Mn, Fe). The microbial status of the fermented liquid manure was also enumerated (bacteria, fungi, actinobacteria). Physiochemical properties divulged that most of the formulations had an acidic pH except a few. The analysis revealed the presence of micronutrients in liquid manures that could promote plant growth. Microbial analysis proved the preparation has more naturally occurring colonies. Mould formation was studied to check the formulation free from spoiling microbes. The results obtained proved the mineral potential of the liquid formulations, which may replenish the soil's fertility and promote plant growth.

Seismic study and spatial observations of a & b – values for the different earthquake hazard zones of India

This paper study the recent seismicity in Earthquake hazard zones in India. A large historical earthquake event catalog to cover the period of 1900-2018, the parameters date, time, latitude, longitude, depth and magnitude has been used to calculating frequency-magnitude distribution (b-value) of seismic hazard zones in India. To convert different magnitude scales into a single moment magnitude scale, the general orthogonal regression relation is used. Gamma distribution used for variable corrections also de-clustering method has used for removal of any non-Poisson distribution. The Indian seismic hazard zones are divided into five major seismic sources zones. The seismicity is characterized by Gutenberg-Richter relation. The parameter ‘b’ of FMD and relationship have been determined for these five seismic zones having different vulnerability environment. The ‘b’ values ranges between 0.43 to 1.16. The difference between the b parameters and seismic hazard level from seismic zones II to V considered for the study of high seismo-tectonic complexity and crustal heterogeneity, the parameter ‘a’ value changes accordingly the seismicity of the regions. The lowest b-values found in seismic zone II. The highest FMD b-value has been found in the seismic zone IV. Such high seismicity b-values may be associated with high heterogeneity. In this high b-value predict the low strength in the crust as well as seismic instabilities of that zone. These observations recommend not suggesting the location of important projects like atomic power stations, hydroelectric power stations, neutrino observatory projects, satellite town projects

Seed germination ability and protein profiling of salt marsh plants at different concentration of sodium chloride

757-765Soil salinity is an increasing problem in agriculture throughout the world. The utilization of halophytic plants for pasture and fodder production in saline soils is the only economic solution presently available. The present study discusses the effects of different concentrations of sodium chloride on seed germination and its impact was determined by protein profiling on Sesuvium portulacastrum, Suaeda maritima and Salicornia brachiata. Seeds germination was substantially delayed and reduced with an increase in NaCl to above threshold level. Changes in the pattern of protein expression were found to be prominent between control and NaCl treated seeds.pproximately 42 kDa, 26 kDa and 20 kDa were found to be up-regulated as the concentration of salt increases in Sesuvium portulacastrum. Whereas, significant variation in the protein patterns were observed in Suaeda maritima such as ~20 to 30 kDa protein bands were not visible and protein band of 55 kDa was particularly increased after 300 mM NaCl treatment. Similarly in Salicornia brachiata expression of 45 kDa protein was up regulated and approximately 25 kDa protein expression was down regulated as the concentration of salt increased to about 1.5 M, 2 M and 2.5 M. However, the upper limit for the survival of the seedling was 200 mM, 300 mM and 1 M for Sesuvium portulacastrum, Suaeda maritima and Salicornia brachiata, respectively. On the basis of the present investigation, this study suggests that optimal application of NaCl can benefit plant growth on stress tolerance studies and also helps for further investigation of the salt tolerance networks

Mitigation of noise-induced bias of PET radiomic features

INTRODUCTION: One major challenge in PET radiomics is its sensitivity to noise. Low signal-to-noise ratio (SNR) affects not only the precision but also the accuracy of quantitative metrics extracted from the images resulting in noise-induced bias. This phantom study aims to identify the radiomic features that are robust to noise in terms of precision and accuracy and to explore some methods that might help to correct noise-induced bias. METHODS: A phantom containing three 18F-FDG filled 3D printed inserts, reflecting heterogeneous tracer uptake and realistic tumor shapes, was used in the study. The three different phantom inserts were filled and scanned with three different tumor-to-background ratios, simulating a total of nine different tumors. From the 40-minute list-mode data, ten frames each for 5 s, 10 s, 30 s, and 120 s frame duration were reconstructed to generate images with different noise levels. Under these noise conditions, the precision and accuracy of the radiomic features were analyzed using intraclass correlation coefficient (ICC) and similarity distance metric (SDM) respectively. Based on the ICC and SDM values, the radiomic features were categorized into four groups: poor, moderate, good, and excellent precision and accuracy. A "difference image" created by subtracting two statistically equivalent replicate images was used to develop a model to correct the noise-induced bias. Several regression methods (e.g., linear, exponential, sigmoid, and power-law) were tested. The best fitting model was chosen based on Akaike information criteria. RESULTS: Several radiomic features derived from low SNR images have high repeatability, with 68% of radiomic features having ICC ≥ 0.9 for images with a frame duration of 5 s. However, most features show a systematic bias that correlates with the increase in noise level. Out of 143 features with noise-induced bias, the SDM values were improved based on a regression model (53 features to excellent and 67 to good) indicating that the noise-induced bias of these features can be, at least partially, corrected. CONCLUSION: To have a predictive value, radiomic features should reflect tumor characteristics and be minimally affected by noise. The present study has shown that it is possible to correct for noise-induced bias, at least in a subset of the features, using a regression model based on the local image noise estimates

Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis

To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-associated transcription factors (TFs) and identify GFRA2 and BST1 as markers of early B cells. Functional analyses in vitro and in vivo as well as single-cell gene expression analyses supported that surface expression of these proteins defined distinct subpopulations that include cells from both the classical common LPs (CLPs) and Fraction A compartments. The formation of the GFRA2-expressing stages of development depended on the TF EBF1, critical both for the activation of stage-specific target genes and modulation of the epigenetic landscape. Our data show that consecutive expression of Ly6D, GFRA2, and BST1 defines a developmental trajectory linking the CLP to the CD19(+) progenitor compartment.Peer reviewe

Case report: Rare skeletal manifestations in a child with primary hyperparathyroidism

Background: Primary hyperparathyroidism (PHPT) is uncommon among children with an incidence of 1:300,000. This diagnosis is often missed in children in contrast to adults where it is detected at a pre symptomatic stage due to routine blood investigations. Etiology of PHPT can be due to adenoma, hyperplasia or rarely carcinoma. Case presentation: A 12year old Sri Lankan girl presented with progressive difficulty in walking since 1year. On examination she had bilateral genu valgum. Skeletal survey revealed valgus deformity of knee joints, bilateral subluxation of upper femoral epiphysis(SUFE), epiphyseal displacement of bilateral humeri, rugger jersey spine and subperiosteal bone resorptions in lateral aspects of 2nd and 3rd middle phalanges. There were no radiological manifestations of rickets. Metabolic profile revealed hypercalcemia with hypophosphatemia. Intact parathyroid hormone levels were elevated at 790pg/ml. Vitamin D levels were deficient. She had low bone mineral density with Z score of -3.4. Vitamin D supplementation resulted in worsening of hypercalcemia without reduction in PTH levels. Tc 99 Sestamibi uptake scan showed abnormal tracer retention in left inferior pole of thyroid. A large parathyroid gland was removed with histology favoring parathyroid adenoma. Post operatively she developed hypocalcemia. Bilateral osteotomy was done for SUFE and further surgeries for correction of limb deformities planned. Conclusion: PHPT in children is usually diagnosed late when irreversible organ damage has occurred. Children can present with non specific symptoms involving gastrointestinal, musculoskeletal, renal and neurological systems. PHPT can cause disarray in bone and epiphysis in children during pubertal growth spurt. Genu valgum and SUFE are rare skeletal manifestations in PHPT and only 10 cases of genu valgum and 9 cases of SUFE have been reported up to now. So far no cases have been reported on epiphyseal displacement of humeri. Awareness regarding the occurrence of these rare skeletal manifestations especially during puberty is important for early diagnosis to prevent irreversible outcomes

SECTM1 Produced by Tumor Cells Attracts Human Monocytes via CD7-Mediated Activation of the PI3K Pathway

Tumor-associated macrophages (TAMs) have essential roles in tumor progression and metastasis. Tumor cells recruit myeloid progenitors and monocytes to the tumor site, where they differentiate into TAMs; however, this process is not well studied in humans. Here we show that human CD7, a T-cell and NK cell receptor, is highly expressed by monocytes and macrophages. Expression of CD7 decreases in M-CSF-differentiated macrophages and in melanoma-conditioned medium–induced macrophages (MCMI/Mφ) in comparison to monocytes. A ligand for CD7, SECTM1 (secreted and transmembrane protein 1), is highly expressed in many tumors, including melanoma cells. We show that SECTM1 binds to CD7 and significantly increases monocyte migration by activation of the PI3K (phosphatidylinositol 3′-kinase) pathway. In human melanoma tissues, tumor-infiltrating macrophages expressing CD7 are present. These melanomas, with CD7-positive inflammatory cell infiltrations, frequently highly express SECTM1, including an N-terminal, soluble form, which can be detected in the sera of metastatic melanoma patients but not in normal sera. Taken together, our data demonstrate that CD7 is present on monocytes and tumor macrophages and that its ligand, SECTM1, is frequently expressed in corresponding melanoma tissues, possibly acting as a chemoattractant for monocytes to modulate the melanoma microenvironment

Expression and DNA methylation of TNF, IFNG and FOXP3 in colorectal cancer and their prognostic significance.

BACKGROUND: Colorectal cancer (CRC) progression is associated with suppression of host cell-mediated immunity and local immune escape mechanisms. Our aim was to assess the immune function in terms of expression of TNF, IFNG and FOXP3 in CRC. METHODS: Sixty patients with CRC and 15 matched controls were recruited. TaqMan quantitative PCR and methylation-specific PCR was performed for expression and DNA methylation analysis of TNF, IFNG and FOXP3. Survival analysis was performed over a median follow-up of 48 months. RESULTS: TNF was suppressed in tumour and IFNG was suppressed in peripheral blood mononuclear cells (PBMCs) of patients with CRC. Tumours showed enhanced expression of FOXP3 and was significantly higher when tumour size was >38 mm (median tumour size; P=0.006, Mann-Whitney U-test). Peripheral blood mononuclear cell IFNG was suppressed in recurrent CRC (P=0.01). Methylated TNFpromoter (P=0.003) and TNFexon1 (P=0.001) were associated with significant suppression of TNF in tumours. Methylated FOXP3cpg was associated with significant suppression of FOXP3 in both PBMC (P=0.018) and tumours (P=0.010). Reduced PBMC FOXP3 expression was associated with significantly worse overall survival (HR=8.319, P=0.019). CONCLUSIONS: We have detected changes in the expression of immunomodulatory genes that could act as biomarkers for prognosis and future immunotherapeutic strategies