A robust high-sensitivity algorithm for automated detection of proteins in two-dimensional electrophoresis gels

The automated interpretation of two-dimensional gel electrophoresis images used in protein separation and analysis presents a formidable problem in the detection and characterization of ill-defined spatial objects. We describe in this paper a hierarchical algorithm that provides a robust, high-sensitivity solution to this problem, which can be easily adapted to a variety of experimental situations. The software implementation of this algorithm functions as part of a complete package designed for general protein gel analysis applications

Changes in the polar vortex: Effects on Antarctic total ozone observations at various stations

October mean total column ozone data from four Antarctic stations form the basis for understanding the evolution of the ozone hole since 1960. While these stations show similar emergence of the ozone hole from 1960 to 1980, the records are divergent in the last two decades. The effects of long-term changes in vortex shape and location are considered by gridding the measurements by equivalent latitude. A clear eastward shift of the mean position of the vortex in October with time is revealed, which changes the fraction of ozone measurements taken inside/outside the vortex for stations in the vortex collar region. After including only those measurements made inside the vortex, ozone behavior in the last two decades at the four stations is very similar. This suggests that dynamical influence must be considered when interpreting and intercomparing ozone measurements from Antarctic stations for detecting ozone recovery and ozone-related changes in Antarctic climate

Hopf algebras: motivations and examples

This paper provides motivation as well as a method of construction for Hopf algebras, starting from an associative algebra. The dualization technique involved relies heavily on the use of Sweedler's dual

A qualitative study of the development of a multidisciplinary case conference review methodology to reduce involved margins in pelvic exenteration surgery for recurrent rectal cancer

Aim Pelvic exenteration surgery remains the only curative option for recurrent rectal cancer. Microscopically involved surgical margins (R1) are associated with a higher risk of local recurrence and decreased survival. Our study aimed to develop a post hoc multidisciplinary case conference review and investigate its potential for identifying areas for improvement. Method Results Patients who underwent pelvic exenteration surgery for recurrent rectal cancer with R1 resections at a tertiary referral centre between April 2014 and January 2016 were retrospectively reviewed from a prospectively maintained database. Patients with non-rectal cancers or who underwent palliative surgery were excluded. Cases, imaging and histopathology were evaluated by a dedicated panel including colorectal surgeons, an abdominal radiologist and a gastrointestinal pathologist. R1 resections were reported in 32 of 110 pelvic exenterations. Patients with other tumours were excluded and one patient had a palliative resection. Nine male patients with 11 exenterations were included with a median age of 56 years. All patients had positive soft tissue margins, and one patient also had an involved bony margin. Failures were due to (interdisciplinary) communication problems, specific management of tumour biology (multifocality, spiculated tumours), which can lead to radiological undercalling, and inadequate surgical technical planning. In hindsight, surgery would have been withheld from one patient. Conclusion A retrospective multidisciplinary case evaluation of pelvic exenteration patients with involved surgical margins led to a list of recommendations which included the need to plan for wider surgical soft tissue resections and improvement in interdisciplinary communication. Lessons learned may increase clear margin rates in future resections

Generalized Bargmann functions, their growth and von Neumann lattices

Generalized Bargmann representations which are based on generalized coherent states are considered. The growth of the corresponding analytic functions in the complex plane is studied. Results about the overcompleteness or undercompleteness of discrete sets of these generalized coherent states are given. Several examples are discussed in detail.Comment: 9 pages, changes with respect to previous version: typos removed, improved presentatio

A product formula and combinatorial field theory

We treat the problem of normally ordering expressions involving the standard boson operators a, ay where [a; ay] = 1. We show that a simple product formula for formal power series | essentially an extension of the Taylor expansion | leads to a double exponential formula which enables a powerful graphical description of the generating functions of the combinatorial sequences associated with such functions | in essence, a combinatorial eld theory. We apply these techniques to some examples related to specic physical Hamiltonians

Hopf Algebras in General and in Combinatorial Physics: a practical introduction

This tutorial is intended to give an accessible introduction to Hopf algebras. The mathematical context is that of representation theory, and we also illustrate the structures with examples taken from combinatorics and quantum physics, showing that in this latter case the axioms of Hopf algebra arise naturally. The text contains many exercises, some taken from physics, aimed at expanding and exemplifying the concepts introduced

Entanglement and nonclassicality for multi-mode radiation field states

Nonclassicality in the sense of quantum optics is a prerequisite for entanglement in multi-mode radiation states. In this work we bring out the possibilities of passing from the former to the latter, via action of classicality preserving systems like beamsplitters, in a transparent manner. For single mode states, a complete description of nonclassicality is available via the classical theory of moments, as a set of necessary and sufficient conditions on the photon number distribution. We show that when the mode is coupled to an ancilla in any coherent state, and the system is then acted upon by a beamsplitter, these conditions turn exactly into signatures of NPT entanglement of the output state. Since the classical moment problem does not generalize to two or more modes, we turn in these cases to other familiar sufficient but not necessary conditions for nonclassicality, namely the Mandel parameter criterion and its extensions. We generalize the Mandel matrix from one-mode states to the two-mode situation, leading to a natural classification of states with varying levels of nonclassicality. For two--mode states we present a single test that can, if successful, simultaneously show nonclassicality as well as NPT entanglement. We also develop a test for NPT entanglement after beamsplitter action on a nonclassical state, tracing carefully the way in which it goes beyond the Mandel nonclassicality test. The result of three--mode beamsplitter action after coupling to an ancilla in the ground state is treated in the same spirit. The concept of genuine tripartite entanglement, and scalar measures of nonclassicality at the Mandel level for two-mode systems, are discussed. Numerous examples illustrating all these concepts are presented.Comment: Latex, 46 page

A Comparative Study of the Common Protozoan Parasites of Clarias gariepinus from the Wild and Cultured Environments in Benue State, Nigeria

A total of one hundred and twenty Clarias gariepinus comprising 30 dead and 30 live fishes were examined for protozoan parasites infestation, sixty each from the wild and a pond (cultured environment) over a period of six months. Ichthyophthirius multifiliis was the most common protozoan parasites found in C. gariepinus from the wild (River Benue) and cultured (pond) environments. These protozoan parasites constitute 37.08% of the total parasites encountered for fishes in the pond and 42.51% of fishes in the wild. Among the body parts of the sampled fishes from the pond, the gills had the highest parasite load (38.86%). Also, the gills had the highest parasite load (40.54%) among the body parts of the fishes sampled from the wild. Fishes not infested with any protozoan parasites from the pond constituted 36.70% of the total fish sampled. On the other hand, fishes not infested with any protozoan parasites from the wild constituted 31.65% of the total fish sampled. Female fishes had more protozoan parasites than the male fishes. Bigger fishes of total length (25–48 cm) had more parasite load than the smaller ones (19–24 cm). Also, fishes between 150–750 g had more parasite load than the smaller ones of less than 150 g. Protozoan parasite load of fish from the cultured environment (pond) did not differ significantly (P < 0.05) from those from River Benue (wild)