Follow-up monitoring in a cat with leishmaniosis and coinfections with Hepatozoon felis and ‘Candidatus Mycoplasma haemominutum’

Case summary A 6-year-old female neutered domestic shorthair cat from Cyprus was presented with multiple ulcerated skin nodules. Cytology and histopathology of the lesions revealed granulomatous dermatitis with intracytoplasmic organisms, consistent with amastigotes of Leishmania species. Biochemistry identified a mild hyperproteinaemia. Blood extraction and PCR detected Leishmania species, Hepatozoon species and ‘Candidatus Mycoplasma haemominutum’ (CMhm) DNA. Subsequent sequencing identified Hepatozoon felis. Additionally, the rRNA internal transcribed spacer 1 locus of Leishmania infantum was partially sequenced and phylogeny showed it to cluster with species derived from dogs in Italy and Uzbekistan, and a human in France. Allopurinol treatment was administered for 6 months. Clinical signs resolved in the second month of treatment with no deterioration 8 months post-treatment cessation. Quantitative PCR and ELISA were used to monitor L infantum blood DNA and antibody levels. The cat had high L infantum DNA levels pretreatment that gradually declined during treatment but increased 8 months post-treatment cessation. Similarly, ELISA revealed high levels of antibodies pretreatment, which gradually declined during treatment and increased slightly 8 months post-treatment cessation. The cat remained PCR positive for CMhm and Hepatozoon species throughout the study. There was no clinical evidence of relapse 24 months post-treatment. Relevance and novel information To our knowledge, this is the first clinical report of a cat with leishmaniosis with H felis and CMhm coinfections. The high L infantum DNA levels post-treatment cessation might indicate that although the lesions had resolved, prolonged or an alternative treatment could have been considere

Evaluation of a two-phase extraction system of carbohydrates and proteins from chlorella vulgaris utex 1803

Microalgae are a valuable source of high-value products and biofuels, however the high-energy cost required for the extraction of their metabolites has kept questioning on possible industrial upgrading. The aim of this study was to evaluate the effect of temperature, solvent/biomass, NaOH concentration and thermal pretreatment of the biomass in a 2-cycle carbohydrate and protein extraction system. Results shown that best conditions for carbohydrates extraction are achieved at a solvent concentration of 3.67 M, 55°C and a solvent/biomass ratio of 30mL/g. On the other side, the best conditions for protein were 3 M, 85°C and 45 mL/g. The efficiencies achieved under these conditions were 95% for carbohydrates and 98% for proteins. Using the best extraction conditions for each metabolite a thermal pre-treatment was performed at 25°C, 75°C and 105°C. Results indicate that highest efficiencies were achieved with dry biomass pretreated at 105°C, with values of 95% for carbohydrates and 98% for proteins

Diving into the mouse macrophage cytoskeletal proteome upon the interaction with candida albicans

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Total serum IgD from healthy and sick dogs with leishmaniosis

This study was supported by Spanish ministry grants, Ministerio de Economía y competitividad and Fondo Europeo de Desarrollo Regional (FEDER, EU) (AGL2012‑32498 and AGL2015‑68477).Background: Canine leishmaniosis (CanL) due to Leishmania infantum is characterized by the development of both cellular and humoral immune responses. The dysfunction of T cell-mediated immunity leads to a lack of proliferation of T cells in response to Leishmania antigens with the consequence of parasite dissemination that seems to be related to a T cell exhaustion mediated by regulatory B cells expressing immunoglobulin D (IgD). The aim of this study was to determine and compare the total serum IgD in dogs with clinical leishmaniosis and in clinically healthy dogs. Results: A total of 147 dog sera were studied. All dogs were tested for L. infantum-specific antibodies by quantitative ELISA. Interferon-gamma (IFN-γ) production was also determined by sandwich ELISA after blood stimulation with L. infantum soluble antigen (LSA) or concanavalin A (ConA). The quantification of total IgD was performed using a human IgD sandwich ELISA quantification set. Dogs were classified in three different groups. Group 1 included 40 clinically healthy non-infected dogs, all serologically negative to L. infantum-specific antibodies and non-producers of IFN-γ upon LSA stimulation. Group 2 included 63 clinically healthy infected dogs that were LSA IFN-γ producers (n = 61) and/or IFN-γ non-producers (n = 2) as well as negative to medium seropositive to L. infantum antigen. Finally, Group 3 included 44 dogs with clinical leishmaniosis (IFN-γ producers, n = 23; and IFN-γ non-producers, n = 21) that were negative to highly positive to L. infantum-specific antibodies. No significant differences were observed when the total IgD concentration was compared within groups. Additionally, total IgD of sick IFN-γ producers and IFN-γ non-producers was not significantly different. Finally, total IgD concentration was not statistically related to demographic parameters such as age, sex and breed. Conclusions: The results of this study demonstrated that there were no differences between groups in total serum IgD. Total serum IgD does not appear to be a marker of disease in CanL

Education and innovation - vet students into technology companies

VET education is an effective and accessible way for students, to improve their employability and inclusion possibilities. It allows them to train technically in a short time to do a job and try to access the labor market. However, not even obtaining a qualification aimed at vocational training ensures access to the labor market or real possibilities of emancipation. The success of VET graduates in their first work experience joining technology companies requires the development of many skills that are common to all professional careers, but also specific skills that derive from the innovative nature of the work method and objectives of these companies. For this reason, within the framework of the Erasmus + Program, the project VET STUDENTS İNTO TECHNOLOGY COMPANİES: A VET students mobility network in the technological sector through a virtual environment with specific materials for critical thinking is being developed with the aim of promoting employability and inclusion of VET students at risk of exclusion. The project focuses on the creation of virtual tools, collaborative work methodologies and virtual training materials that allow these young people to improve their critical thinking capacity and their technological and digital skills. The project's methodology is based on the combination of the creation of training materials and a network of mobility in high-tech centres, where students will carry out a first work experience. Regarding training materials, one of the main results of this project is PERSONALIZED DIGITAL COURSE FOR LABOR TRAINING IN A TECHNOLOGICAL CENTER. This course generates personalized learning based on the student's previous knowledge and the characteristics of the technological company in which they will carry out their internship. The course follows an innovative methodology based on microlearning, a learning characterized by the resolution of relatively small exercises (pills) that are focused on the development of technical and work skills and the reinforcement of critical thinking as a fundamental mechanism. The objective of this course is not so much to provide new knowledge as to develop skills that allow the students to face problems or tasks unknown to them until then: self-training skills, assess their own way of reasoning, set objectives, raise relevant questions, state hypothesis, search for information, intellectual integrity, oral and written communication, work in multidisciplinary teams. Along with this course, within the framework of this project, the result ON YOUR SIDE is being developed, a digital educational software available for mobile devices, which will accompany the student throughout the mobility process, providing useful information such as specific training resources focused on the company where they will carry out their mobility, linguistic resources, collaborative work tools, as well as social integration resources in the destination country, among others. From this App, students will be able to access the aforementioned course. In this paper we present how, through microlearning methodology and critical thinking, students can solve the content pills through their autonomy and self-discovery.This work has been funded by the Erasmus + program within the 2019-1-ES01-KA202-064569 project, Spanish Service for the Internationalization of Education (SEPIE

Band termination in the N=Z Odd-Odd Nucleus 46V

High spin states in the odd-odd N=Z nucleus 46V have been identified. At low spin, the T=1 isobaric analogue states of 46Ti are established up to I = 6+. Other high spin states, including the band terminating state, are tentatively assigned to the same T=1 band. The T=0 band built on the low-lying 3+ isomer is observed up to the 1f7/2-shell termination at I=15. Both signatures of a negative parity T=0 band are observed up to the terminating states at I = 16- and I = 17-, respectively. The structure of this band is interpreted as a particle-hole excitation from the 1d3/2 shell. Spherical shell model calculations are found to be in excellent agreement with the experimental results.Comment: 5 pages, 4 figure

Exploring the Relationship Between Susceptibility to Canine Leishmaniosis and anti-Phlebotomus Perniciosus Saliva Antibodies in Ibizan Hounds and Dogs of Other Breeds in Mallorca, Spain

Background: Canine leishmaniosis caused by Leishmania infantum is a neglected zoonosis transmitted by sand flies like Phlebotomus perniciosus. Clinical signs and disease susceptibility vary according to various factors, including host immune response and breed. In particular, Ibizan hounds appear more resistant. This immunocompetence could be attributed to a more frequent exposure to uninfected sand flies, eliciting a stronger anti-sand fly saliva antibody response. Methods: This study aimed to investigate the prevalence of anti-P. perniciosus saliva antibodies in Ibizan hounds and dogs of other breeds in the Leishmania-endemic area of Mallorca, Spain, and to correlate these antibody levels with clinical, immunological and parasitological parameters. Anti-sand fly saliva IgG was examined in 47 Ibizan hounds and 45 dogs of other breeds using three methods: P. perniciosus whole salivary gland homogenate (SGH) ELISA; recombinant protein rSP03B ELISA; and rSP03B rapid tests (RT). Additionally, diagnostic performance was evaluated between methods. Results: Results indicate significantly higher anti-SGH antibodies (P = 0.0061) and a trend for more positive SGH ELISA and RT results in Ibizan hounds compared to other breeds. General linear model analysis also found breed to be a significant factor in SGH ELISA units and a marginally significant factor in RT result. Although infection rates were similar between groups, Ibizan hounds included significantly more IFN-γ producers (P = 0.0122) and papular dermatitis cases (P < 0.0001). Older age and L. infantum seropositivity were also considered significant factors in sand fly saliva antibody levels according to at least one test. Fair agreement was found between all three tests, with the highest value between SGH and rSP03B RT. Conclusions: To our knowledge, this is the first study elaborating the relationship between anti-P. perniciosus saliva antibodies and extensive clinical data in dogs in an endemic area. Our results suggest that Ibizan hounds experience a higher frequency of exposure to sand flies and have a stronger cellular immune response to L. infantum infection than other breed dogs. Additional sampling is needed to confirm results, but anti-P. perniciosus saliva antibodies appear to negatively correlate with susceptibility to L. infantum infection and could possibly contribute to the resistance observed in Ibizan hounds

VET students into technology companies: a microlearning digital course to train critical thinking skills

This microlearning course is designed to support VET graduates or students, especially those with fewer opportunities, who join a technology company at the beginning of their professional career. It has been developed within the framework of the Erasmus + project “VET STUDENTS İNTO TECHNOLOGY COMPANİES: A VET students mobility network in the technological sector through a virtual environment with specific materials for critical thinking”. This is an intiative co-financed by the Erasmus + Programme, with a personalized digital course format, to take into account both the previous training of the VET student or graduate and the type of company they will join. The course has in its sights to develop in the VET student the specific competences required by the technology company to address the problems typical of fields of leading technology innovation, as well as fitting in multidisciplinary teams. To do this, this course generates an innovative methodology in which the education itinerary is built based on previous knowledge of the student and the needs and work field of the technology company. Besides its contents strengthen critical thinking as an essential mechanism for work in both dynamic and technology sectors. For that purpose, the student is asked a series of questions in the form of problems that, in addition to being related to the study plans of VET studies, require for their solution the search for information, its analysis and many times the making of a decision or adopting a personal position on it. We aim with our training projects, within the Erasmus + program, to convince students that they are capable of going further and further in the development of their professional skills being the technology sector an acceptable option for them, making their work increasingly valuable for their inclusion, for their own personal development, for that of the company in which they work and for society. But for this certain skills are required, and one of the most important is learning to learn autonomously and to accurately analyse the information received and the decisions that are made from it. It is generally true that the problems that one encounters in professional practice are not going to be the ones that you were taught in your VET studies. Vocational training has to head for equipping students with the necessary tools to face the new problems that they will face in their job and to grow professionally every day. We intend to show how valuable critical thinking skills are in analysing our way of thinking and making decisions. In this paper we present examples of the exercises included in the course, how their approach and the processes that have led to a solution relate with the standards of critical thinking and the different levels of help that are offered to the student for their solution. The course is accessible from a digital educational software “ON YOUR SIDE: VIRTUAL ASSISTANT FOR EUROPEAN MOBILITIES IN TECHNOLOGICAL CENTERS” developed in the framework of this Erasmus+ project in order to develop cooperative work between students with fewer opportunities doing their internships in technology companies and their inclusion in new job posts. We will also present this software in a very summarized way in this article.This work has been funded by the Erasmus + program within the 2019-1-ES01-KA202-064569 project, Spanish Service for the Internationalization of Education (SEPIE

Optimal design of irrigation network shifts and characterization of their flexibility

[EN] In this contribution, a nonlinear programming genetic hybrid algorithm for the optimal design of pressurized irrigation networks operating in shifts is presented, and an indicator is implemented to evaluate the flexibility of the resulting designs of the hybrid algorithm. The main objective is the minimization of the economic cost of the pipe network and the evaluation of the flexibility of the system designs. The research considers the allocation of shifts to the irrigation outlets (hydrants) and the sizing of the pipes as decision variables to reduce implementation costs. With the results of the optimization, the flexibility of the hydraulic network is analysed based on the random reassignment of shifts to the hydrants, which simulates the modifications that may occur during network operation. To validate the algorithm, once calibrated, it was applied to the design of four real irrigation sectors of Ecuador and Spain. The results indicate that the designs obtained improve in cost and flexibility with respect to those found with the traditional algorithm of the Economic Series, and that the assignment of the shifts is of utmost importance to minimize investment costs in pipes. At the same time, it constitutes a determining factor in the flexibility of the system[ES] En esta contribución se presenta un algoritmo híbrido genéticoprogramación no lineal para el diseño óptimo de redes presurizadas de riego operando a turnos, y se implementa un indicador para evaluar la flexibilidad de los diseños resultantes del algoritmo híbrido. El objetivo principal es la minimización del costo económico de la red de tuberías y la evaluación de la flexibilidad de los diseños del sistema. La investigación considera como variables de decisión la asignación de turnos a las tomas de riego (hidrantes) y el dimensionado de las conducciones para reducir los costos de implementación. Con los resultados de la optimización se analiza la flexibilidad de la red hidráulica con base en la reasignación aleatoria de turno a los hidrantes, que simula las modificaciones susceptibles de ocurrir durante la explotación de la red. Para validar el algoritmo, una vez calibrado, éste fue aplicado al diseño de cuatro sectores de riego reales de Ecuador y España. Los resultados indican que los diseños obtenidos mejoran en costo y flexibilidad respecto a los encontrados con el algoritmo tradicional de la serie económica, y que la asignación de los turnos es de suma importancia para minimizar los costos de inversión en tuberías, lo que a la vez constituye un factor determinante en la flexibilidad del sistemaLapo P., CM.; Pérez-García, R.; Aliod-Sebastián, R.; Martínez-Solano, FJ. (2020). Diseño óptimo de redes de riego a turnos y caracterización de su flexibilidad. Tecnología y Ciencias del Agua (Online). 11(1):266-314. https://doi.org/10.24850/j-tyca-2020-01-07S26631411