Reduced Necrosis and Content of Apoptotic M1 Macrophages in Advanced Atherosclerotic Plaques of Mice With Macrophage-Specific Loss of Trpc3

In previous work we reported that ApoeKO mice transplanted with bone marrow cells deficient in the Transient Receptor Potential Canonical 3 (TRPC3) channel have reduced necrosis and number of apoptotic macrophages in advanced atherosclerotic plaques. Also, in vitro studies with polarized macrophages derived from mice with macrophage-specific loss of TRPC3 showed that M1, but not M2 macrophages, deficient in Trpc3 are less susceptible to ER stress-induced apoptosis than Trpc3 expressing cells. The questions remained (a) whether the plaque phenotype in transplanted mice resulted from a genuine effect of Trpc3 on macrophages, and (b) whether the reduced necrosis and macrophage apoptosis in plaques of these mice was a manifestation of the selective effect of TRPC3 on apoptosis of M1 macrophages previously observed in vitro. Here, we addressed these questions using Ldlr knockout (Ldlr−/−) mice with macrophage-specific loss of Trpc3 (MacTrpc3−/−/Ldlr−/− → Ldlr−/−). Compared to controls, we observed decreased plaque necrosis and number of apoptotic macrophages in MacTrpc3−/−/Ldlr−/− → Ldlr−/− mice. Immunohistochemical analysis revealed a reduction in apoptotic M1, but not apoptotic M2 macrophages. These findings confirm an effect of TRPC3 on plaque necrosis and support the notion that this is likely a reflection of the reduced susceptibility of Trpc3-deficient M1 macrophages to apoptosis.Fil: Solanki, Sumeet. University of Toledo; Estados UnidosFil: Dube, Prabhatchandra R.. University of Toledo; Estados UnidosFil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Vazquez, Guillermo. University of Toledo; Estados Unido

Fluxtube model atmospheres and Stokes V zero-crossing wavelengths

First results of the inversion of Stokes I and V profiles from plage regions near disk center are presented. Both low and high spatial resolution spectra of FeI 6301.5 and FeI 6302.5 A obtained with the Advanced Stokes Polarimeter (ASP) have been considered for analysis. The thin flux tube approximation, implemented in an LTE inversion code based on response functions, is used to describe unresolved magnetic elements. The code allows the simultaneous and consistent inference of all atmospheric quantities determining the radiative transfer with the sole assumption of hydrostatic equilibrium. By considering velocity gradients within the tubes we are able to match the full ASP Stokes profiles. The magnetic atmospheres derived from the inversion are characterized by the absence of significant motions in high layers and strong velocity gradients in deeper layers. These are essential to reproduce the asymmetries of the observed profiles. Our scenario predicts a shift of the Stokes V zero-crossing wavelengths which is indeed present in observations made with the Fourier Transform Spectrometer.Comment: To appear in ApJ Letters (1997) (in press

Simulations Show that Vortex Flows could Heat the Chromosphere in Solar Plage

The relationship between vortex flows at different spatial scales and their contribution to the energy balance in the chromosphere is not yet fully understood. We perform three-dimensional (3D) radiation-magnetohydrodynamic (MHD) simulations of a unipolar solar plage region at a spatial resolution of 10 km using the MURaM code. We use the swirling-strength criterion that mainly detects the smallest vortices present in the simulation data. We additionally degrade our simulation data to smooth-out the smaller vortices, so that also the vortices at larger spatial scales can be detected. Vortex flows at various spatial scales are found in our simulation data for different effective spatial resolutions. We conclude that the observed large vortices are likely clusters of much smaller ones that are not yet resolved by observations. We show that the vertical Poynting flux decreases rapidly with reduced effective spatial resolutions and is predominantly carried by the horizontal plasma motions rather than vertical flows. Since the small-scale horizontal motions or the smaller vortices carry most of the energy, the energy transported by vortices deduced from low resolution data is grossly underestimated. In full resolution simulation data, the Poynting flux contribution due to vortices is more than adequate to compensate for the radiative losses in plage, indicating their importance for chromospheric heating.Comment: 8 pages, 5 figures, accepted in ApJ

Waves as the source of apparent twisting motions in sunspot penumbrae

The motion of dark striations across bright filaments in a sunspot penumbra has become an important new diagnostic of convective gas flows in penumbral filaments. The nature of these striations has, however, remained unclear. Here we present an analysis of small scale motions in penumbral filaments in both simulations and observations. The simulations, when viewed from above, show fine structure with dark lanes running outwards from the dark core of the penumbral filaments. The dark lanes either occur preferentially on one side or alternate between both sides of the filament. We identify this fine structure with transverse (kink) oscillations of the filament, corresponding to a sideways swaying of the filament. These oscillations have periods in the range of 5-7 min and propagate outward and downward along the filament. Similar features are found in observed G-band intensity time series of penumbral filaments in a sunspot located near disk center obtained by the Broadband Filter Imager (BFI) on board {\it Hinode}. We also find that some filaments show dark striations moving to both sides of the filaments. Based on the agreement between simulations and observations we conclude that the motions of these striations are caused by transverse oscillations of the underlying bright filaments.Comment: Accepted for publication in Astrophysical Journal on 8th April 201

Nonlinear Force-Free Field Modeling of the Solar Magnetic Carpet and Comparison with SDO/HMI and Sunrise/IMaX Observations

In the quiet solar photosphere, the mixed polarity fields form a magnetic carpet, which continuously evolves due to dynamical interaction between the convective motions and magnetic field. This interplay is a viable source to heat the solar atmosphere. In this work, we used the line-of-sight (LOS) magnetograms obtained from the Helioseismic and Magnetic Imager (HMI) on the \textit{Solar Dynamics Observatory} (\textit{SDO}), and the Imaging Magnetograph eXperiment (IMaX) instrument on the \textit{Sunrise} balloon-borne observatory, as time dependent lower boundary conditions, to study the evolution of the coronal magnetic field. We use a magneto-frictional relaxation method, including hyperdiffusion, to produce time series of three-dimensional (3D) nonlinear force-free fields from a sequence of photospheric LOS magnetograms. Vertical flows are added up to a height of 0.7 Mm in the modeling to simulate the non-force-freeness at the photosphere-chromosphere layers. Among the derived quantities, we study the spatial and temporal variations of the energy dissipation rate, and energy flux. Our results show that the energy deposited in the solar atmosphere is concentrated within 2 Mm of the photosphere and there is not sufficient energy flux at the base of the corona to cover radiative and conductive losses. Possible reasons and implications are discussed. Better observational constraints of the magnetic field in the chromosphere are crucial to understand the role of the magnetic carpet in coronal heating.Comment: Accepted for publication in The Astrophysical Journal (13 pages, 10 figures

Canning of smoked eel

On an average about 5000metric tons of marine eels are landed every year in India. Even though it is a quality fish with high protein content as any other popular species, many fish eaters decline to prefer it to other low quality fish. One way to utilise this fish is to convert it into various products like smoked and canned eel fillets. As this product is likely to secure a foreign market also, investigations were carried out with a view to suggesting a proper method for its preparation. The data collected on this line are presented here, applying which an excellent smoked and canned product from eel can be turned out

Fan Loops Observed by IRIS, EIS and AIA

A comprehensive study of the physical parameters of active region fan loops is presented using the observations recorded with the Interface Region Imaging Spectrometer (IRIS), the EUV Imaging Spectrometer (EIS) on-board Hinode and the Atmospheric Imaging Assembly (AIA) and the Helioseismic and Magnetic Imager (HMI) on-board the Solar Dynamics Observatory (SDO). The fan loops emerging from non-flaring AR~11899 (near the disk-center) on 19th November, 2013 are clearly discernible in AIA 171~{\AA} images and those obtained in \ion{Fe}{8} and \ion{Si}{7} images using EIS. Our measurements of electron densities reveal that the footpoints of these loops are approximately at constant pressure with electron densities of $\log\,N_{e}=$ 10.1 cm$^{-3}$ at $\log\,[T/K]=5.15$ (\ion{O}{4}), and $\log\,N_{e}=$ 8.9 cm$^{-3}$ at $\log\,[T/K]=6.15$ (\ion{Si}{10}). The electron temperature diagnosed across the fan loops by means of EM-Loci suggest that at the footpoints, there are two temperature components at $\log\,[T/K]=4.95$ and 5.95, which are picked-up by IRIS lines and EIS lines respectively. At higher heights, the loops are nearly isothermal at $\log\,[T/K]=5.95$, that remained constant along the loop. The measurement of Doppler shift using IRIS lines suggests that the plasma at the footpoints of these loops is predominantly redshifted by 2-3~km~s$^{-1}$ in \ion{C}{2}, 10-15~km~s$^{-1}$ in \ion{Si}{4} and $~$15{--}20~km~s$^{-1}$ in \ion{O}{4}, reflecting the increase in the speed of downflows with increasing temperature from $\log\,[T/K]=4.40$ to 5.15. These observations can be explained by low frequency nanoflares or impulsive heating, and provide further important constraints on the modeling of the dynamics of fan loops.Comment: Accepted for publication in The Astrophysical Journal; 8 Figures, 11 page

Migration of Ca II H bright points in the internetwork

The migration of magnetic bright point-like features (MBP) in the lower solar atmosphere reflects the dispersal of magnetic flux as well as the horizontal flows of the atmospheric layer they are embedded in. We analyse trajectories of the proper motion of intrinsically magnetic, isolated internetwork Ca II H MBPs (mean lifetime 461 +- 9 s) to obtain their diffusivity behaviour. We use seeing-free high spatial and temporal resolution image sequences of quiet-Sun, disc-centre observations obtained in the Ca II H 3968 {\AA} passband of the Sunrise Filter Imager (SuFI) onboard the Sunrise balloon-borne solar observatory. Small MBPs in the internetwork are automatically tracked. The trajectory of each MBP is then calculated and described by a diffusion index ({\gamma}) and a diffusion coefficient (D). We further explore the distribution of the diffusion indices with the help of a Monte Carlo simulation. We find {\gamma} = 1.69 +- 0.08 and D = 257 +- 32 km^2/s averaged over all MBPs. Trajectories of most MBPs are classified as super-diffusive, i.e., {\gamma} > 1, with the determined {\gamma} being to our knowledge the largest obtained so far. A direct correlation between D and time-scale ({\tau}) determined from trajectories of all MBPs is also obtained. We discuss a simple scenario to explain the diffusivity of the observed, relatively short-lived MBPs while they migrate within a small area in a supergranule (i.e., an internetwork area). We show that the scatter in the {\gamma} values obtained for individual MBPs is due to their limited lifetimes. The super-diffusive MBPs can be well-described as random walkers (due to granular evolution and intergranular turbu- lence) superposed on a large systematic (background) velocity, caused by granular, mesogranular and supergranular flows.Comment: 10 pages, 7 figures, 3 table

Facile fabrication of lateral nanowire wrap-gate devices with improved performance

We present a simple fabrication technique for lateral nanowire wrap-gate devices with high capacitive coupling and field-effect mobility. Our process uses e-beam lithography with a single resist-spinning step, and does not require chemical etching. We measure, in the temperature range 1.5-250 K, a subthreshold slope of 5-54 mV/decade and mobility of 2800-2500 $cm^2/Vs$ -- significantly larger than previously reported lateral wrap-gate devices. At depletion, the barrier height due to the gated region is proportional to applied wrap-gate voltage.Comment: 3 pages, 3 figure