Genetic structure of gilthead seabream, Sparus aurata, in the Central Mediterranean Sea

Abstract The gilthead seabream, Sparus aurata, represents an important economic resource for Mediterranean aquaculture. In spite of its wide geographic distribution and economic importance, only recently studies have been carried out on the genetic composition of natural populations, which have revealed a picture of a heterogeneous degree of genetic differentiation among S. aurata populations. In this study an allozyme analysis of samples from six different collecting sites along the Italian and Croatian coasts was carried out, covering an area in the Central Mediterranean sea that has yet to be investigated through gene-enzyme systems. Data on 26 gene loci, 10 of which are polymorphic, indicate a slight but significant genetic structure (FST = 0.0167) of the species. A hierarchical analysis of population subdivision made it possible to identify three different assemblages found in the Adriatic Sea, Tyrrhenian Sea and Sardinian Channel, though an isolation by distance model can be rejected. The results are discussed in the light of previous literature and taking conservation into consideration

Cytogenetic studies in Sparus auratus

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Deciphering a multi-event in a non-complex set of detrital zircon U–Pb ages from Carboniferous graywackes of SW Iberia

The determination of U–Pb ages from detrital zircons of sedimentary rocks using LA-ICP-MS has been widely used for the purpose of provenance analysis. One problem that frequently arises is finding a population that appears to be non-complex despite several perceptible age peaks in its spectrum. These peaks are qualitatively defined by means of relative probability diagrams, or PDFs, but it is difficult to quantify their statistical significance relative to a zircon forming multi-event. Thus, can a multi-event in a non-complex set of detrital zircon U–Pb ages be deciphered and characterized? The aim of this study is to attempt to provide an answer to this question by means of statistical analysis. Its objectives are: a) to determine the best minimum number of zircon age populations (peaks), BmPs, b) for the characterization of each peak in terms of age and event duration; c) to compare the results obtained from two datasets showing similar zircon ages; and d) to demonstrate the usefulness of deciphering these BmPs. First, cluster analysis is carried out, aimed at grouping zircon ages into a set of consistent clusters. A Gaussian Kernel function is then fitted to each cluster and summed to obtain a theoretical PDFm (modeled probability density function). Finally, the selected modeled PDFm (that built on the BmPs) is that which reports the lowest number of peaks for which the difference as compared with the original gPDF (global probability density function) is equal to or below 5%. Deciphered BmP peaks can be characterized and used for characterizing and providing an understanding of related event(s). A geological interpretation, based on the results obtained, is attempted. This includes a robust measure for maximum age of deposition for both Cabrela and Mértola graywackes

The provenance of Late Ediacaran and Early Ordovician siliciclastic rocks in the

U–Pb geochronology of detrital zircon from Late Ediacaran (Beiras Group greywackes) and Early Ordovician (Sarnelhas arkosic quartzites and Armorican quartzites of Penacova) sedimentary rocks of the southwest Central Iberian Zone (SW CIZ) constrain the evolution of northern Gondwana active-passive margin transition. The LA-ICP-MS U–Pb data set (375 detrital zircons with 90–110% concordant ages) is dominated by Neoproterozoic ages (75% for the greywakes and 60% for the quartzites), among which the main age cluster (more significant for Beiras Group greywackes) is Cryogenian (c.840–750 Ma), while a few Mesoproterozoic and Tonian ages are also present (percentages <8%). These two features, and the predominance of Cryogenian ages over Ediacaran ages, distinguish the Beiras Group greywackes (SW CIZ) from the time-equivalent Serie Negra (Ossa-Morena Zone – OMZ), with which they are in inferred contact. The age spectra of the Beiras Group greywackes also reveal three major episodes of zircon crystallisation in the source area during the Neoproterozoic that are probably associated with a long-lived system of magmatism that developed either along or in the vicinity of the northern Gondwana margin at: (1) c. 850–700 Ma – Pan-African suture (not well represented in OMZ); (2) c. 700–635 Ma – early Cadomian arc; and (3) c. 635–545 Ma – late Cadomian arc. Comparison of Neoproterozoic ages and those of the Paleoproterozoic (c. 2–1.8 Ga) and Archean (mainly Neoarchean – 2.8–2.6 Ga, but also older) in the Beiras Group greywackes with U–Pb ages of Cadomian correlatives shows that: (1) SW CIZ, OMZ, Saxo- Thuringian Zone, North Armorican Cadomian Belt and Anti-Atlas) evolved together during the formation of back-arc basins on the northern Gondwana active margin and (2) all recorded synorogenic basins that were filled during the Ediacaran by detritus resulting from erosion of the West African craton, the Pan- African suture and a long-lived Cadomian magmatic arc. Differences in detrital zircon age populations in the greywackes of the Beiras Group (SW CIZ Cadomian basement) and the Serie Negra (OMZ Cadomian basement) are also observed in their respective overlying Early Ordovician quartzites. Since both these SW Iberia Cadomian basements evolved together along the active margin of Gondwana (but sufficiently separated to account for the differences in their detrital zircon content), this continuation of differing zircon populations into the Early Ordovician suggests that the inferred contact presently juxtaposing the Beiras Group and the Serie Negra is not pre-Early Ordovician and so is unlikely to demonstrate a Cadomian suture

. U-Pb detrital zircon ages from the Beiras Group: Implications for the Neoproterozoic evolution of the SW Iberia

U-Pb detrital zircon ages from the Beiras Group greywackes (SW Central Iberian Zone - CIZ) indicate a maximum depositional age of late Ediacaran (c. 560-578 Ma). Two salient features distinguish the Beiras Group from the Série Negra greywackes (age equivalent from the Ossa-Morena Zone - OMZ): i) The presence of Tonian and Mesoproterozoic (<8%) age clusters in the Beiras Group greywackes, that are almost absent in the OMZ, imply either a distinct or an additional source of detrital zircons from the West African Craton; and 2) The higher content of Cryogenian zircon ages of the Beiras Group greywackes (mainly at c. 840-750 Ma and c. 685-660 Ma), that contrast with the dominant Ediacaran zircon ages of the Série Negra greywackes (OMZ). The Cryogenian zircon forming events that are dominant in the SW CIZ basins are probably related to a different source with early Cadomian juvenile crust (c. 700-635 Ma) and with a possible contribution of the Pan-African suture (c. 850-700 Ma). The Nd isotopic signatures support the addition of a juvenile source to pre-existent older crust for the Beiras Group metasediments. Although the Beiras Group (SW CIZ) and Serie Negra (OMZ) late Ediacaran basins have evolved together in the active margin of Gondwana, they were sufficiently separated to account for the differences in their detrital zircon content and isotopic signatures

Intra-crustal recycling and crustal-mantle interactions in

In situ O-isotope compositions of detrital, inherited and melt-precipitated zircons with Neoproterozoic to Ordovician ages are presented to assess the crustal evolution of the North Gondwana margin. Different groups of pre-Mesozoic rocks from SW Iberia were targeted: i) Ediacaran paragneisses and meta-greywakes of the Ossa-Morena Zone – the Serie Negra Group deposited at ~ 560 Ma in a Cadomian magmatic arc setting (Pereira et al., 2008); ii) Early to Middle Cambrian orthogneisses and volcaniclastic rocks of the Ossa-Morena Zone – Evora Massif igneous complexes related to ensialic rifting at ~ 530–500 Ma (Pereira et al., 2008, Chichorro et al., 2008); iii) Late Cambrian to Early Ordovician volcaniclastic rocks and granites of the Ossa-Morena–Central Iberian transition zone – the Urra Formation and Portalegre granite formed at ~ 495– 488 Ma in a extensional setting (Solá et al., 2008); iv) Carboniferous granitoids (Nisa and Arraiolos granites) containing inherited zircons with Cambrian to Ordovician ages (Solá, this volume). A compilation the results for the period ~ 3.4 Ga to ~ 450 Ma reveals that: a) Archean zircons show little variation in d18O, with most values lying between 4.7 and 7.5‰, (average 6.2‰) comparable with usual d18O of zircons from Archean elsewhere (e.g., Valley et al., 2005); b) the range of d18O in Paleoproterozoic grains increases between 2.1 and 1.8 Ga with d18O >7.5‰, indicating increasing supracrustal recycling, but at ~ 1.8 Ga the d18O has mantle-like values (<5.1‰), documenting a crustal growth episode at this time; c) rare Mesoproterozoic grains have mildly evolved d18O values in the range 5.6–7.1‰); d) Tonian grains have low d18O values (4.2–5.6‰) typical of mantle-derived juvenile magmas but also higher values of 9.9‰ suggesting intra-crustal recycling; e) Cryogenian–Ordovician zircons show more variable and higher d18O values (~4 to >10‰), indicating great diversity and mixing of sources through intra-crustal recycling and crust–mantle interactions; f) some d18O values near to or below mantle composition (5.3 ± 0.3‰) were recorded at ~ 590 Ma (Ediacaran) suggesting input of mantle material into the crust; g) a decrease in variance of d18O occurs from 575 Ma to the Ediacaran/Cambrian boundary, suggesting a relative decrease in the magmatic contribution of surface-derived material; h) in Cambrian times, the average d18O is higher in the 536–520Ma interval (7.0‰) than in the 520–488 interval (6.2‰), which can be taken as a signal of gradual opening of the system to mantle-derived, mafic, rift-related igneous complexes; i) higher values of d18O (>7.5 ‰) recorded at ~ 623–574 Ma and 490–470Ma mark periods of pronounced increase in crustal recycling

New insights from U–Pb zircon dating of Early Ordovician magmatism on

The Central Iberian–Ossa-Morena transition zone (SW Iberian Massif) represents a segment of the northern Gondwana margin with a long geodynamic evolution, characterized by the superposition of Cadomian and Variscan events. The Early Ordovician is mainly represented by porphyritic felsic volcaniclastic rocks (the Urra Formation) that pass up into a siliciclastic sediments typical of the Central Iberian Zone (Lower Ordovician Armorican Quartzite Formation). The Urra Formation unconformably overlies the previously deformed and metamorphosed Ediacaran sediments of the Série Negra (with Ossa-Morena Zone paleogeographic affinity). New SHRIMP zircon data obtained from the Urra Formation volcaniclastic rocks indicate an Early Ordovician age (206Pb/238U ages ranging from 494.6±6.8 Ma to 488.3±5.2 Ma) for this magmatic event. The inherited zircon cores indicate the presence of multicycle protoliths with different Precambrian ages: Neoproterozoic (698–577 Ma), Paleoproterozoic (2.33 Ga) and Paleoarchean (3.2–3.3 Ga). There is a noticeable lack of Meso- to Neoarchean and Mesoproterozoic ages. The data support the hypothesis that the volcaniclastic rocks were derived by partial melting of Cadomian basement (linked to a West African Craton provenance). The Urra Formation volcaniclastic rocks have rhyolitic to dacitic compositions, are peraluminous and similar to calc-alkaline high-K series suites elsewhere. Isotopic signatures present a wide range of values (87Sr/86Sr)t=0.7085–0.7190, more restricted εNdt (−2.65 to −0.35) and δ18O=9.63–10.34‰, compatible with magmas derived from crustal rocks, including portions of the lower crust. Some samples show disturbance of the Rb–Sr system as shown by unrealistic values for (87Sr/86Sr)tb0.703, probably due to Variscan deformation and metamorphism. The volcaniclastic rocks with a significant sedimentary contribution (upper unit) are distinguished from the others by the lowest values of εNdt (−5.53 to −4.85). The geochemical data are compatible with an orogenic geodynamic environment. However, the “orogenic” signature can be considered to represent, in part, an inherited feature caused by melting of the Cadomian basement which also has calc-alkaline affinities. The Early Ordovician crustal growth and associated magmatism, represented by the Urra felsic volcaniclastic rocks and associated calc-alkaline granitoids, diorites and gabbros, can be interpreted in terms of the underplating and temporal storage of mantle-derived magmas as the potential source for the “orogenic melts” that were intruded during Early Paleozoic extension. This record of Early Ordovician magmatism has striking similarities with other correlatives from the Iberian, Bohemian and Armorican massifs that are discussed in this paper. This comparison reinforces the probable existence of a large-scale crustal melting process linked to a significant episode of extension on the northern Gondwana margin that probably resulted in the birth of the Rheic Ocean

Dengue virus targets RBM10 deregulating host cell splicing and innate immune response

RNA-seq experiments previously performed by our laboratories showed enrichment in intronic sequences and alterations in alternative splicing in dengue-infected human cells. The transcript of the SAT1 gene, of well-known antiviral action, displayed higher inclusion of exon 4 in infected cells, leading to an mRNA isoform that is degraded by non-sense mediated decay. SAT1 is a spermidine/spermine acetyl-transferase enzyme that decreases the reservoir of cellular polyamines, limiting viral replication. Delving into the molecular mechanism underlying SAT1 pre-mRNA splicing changes upon viral infection, we observed lower protein levels of RBM10, a splicing factor responsible for SAT1 exon 4 skipping. We found that the dengue polymerase NS5 interacts with RBM10 and its sole expression triggers RBM10 proteasome-mediated degradation. RBM10 over-expression in infected cells prevents SAT1 splicing changes and limits viral replication, while its knock-down enhances the splicing switch and also benefits viral replication, revealing an anti-viral role for RBM10. Consistently, RBM10 depletion attenuates expression of interferon and pro-inflammatory cytokines. In particular, we found that RBM10 interacts with viral RNA and RIG-I, and even promotes the ubiquitination of the latter, a crucial step for its activation. We propose RBM10 fulfills diverse pro-inflammatory, anti-viral tasks, besides its well-documented role in splicing regulation of apoptotic genes.Fil: Pozzi, María Berta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Bragado, Laureano Fabian Tomas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Mammi, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Torti, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Gaioli, Nicolas Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Gebhard, Leopoldo German. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaFil: Garcia Sola, Martin Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Drago, Rita Vaz. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Iglesias, Nestor Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garcia, Cybele. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Gamarnik, Andrea Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Srebrow, Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentin

Regulation of hippocampal postnatal and adult neurogenesis by adenosine A 2A receptor: Interaction with brain-derived neurotrophic factor

21 páginas, 7 figuras.Adenosine A2A receptor (A2A R) activation modulates several brain processes, ranging from neuronal maturation to synaptic plasticity. Most of these actions occur through the modulation of the actions of the neurotrophin brain-derived neurotrophic factor (BDNF). In this work, we studied the role of A2A Rs in regulating postnatal and adult neurogenesis in the rat hippocampal dentate gyrus (DG). Here, we show that A2A R activation with CGS 21680 promoted neural stem cell self-renewal, protected committed neuronal cells from cell death and contributed to a higher density of immature and mature neuronal cells, particularly glutamatergic neurons. Moreover, A2A R endogenous activation was found to be essential for BDNF-mediated increase in cell proliferation and neuronal differentiation. Our findings contribute to further understand the role of adenosinergic signaling in the brain and may have an impact in the development of strategies for brain repair under pathological conditions.Fundaç~ao para a Ciência e a Tecnologia, Grant/Award Numbers: IF/01227/2015, SFRH/BD/74662/2010, IMM/CT/35-2018, SFRH/BD/128280/2017, SFRH/BD/129710/2017; H2020-WIDESPREAD-05-2020-Twinning (EpiEpinet) under grant agreement No, Grant/Award Number: 9524; Ministerio de Ciencia e Innovaci on, Grant/Award Number: PID2019-111225RB-I00; Spanish MICIU, Grant/Award Number: SAF2015-70433-R; Generalitat Valenciana, Grant/Award Number: PROMETEO/2018/055; COST Action, Grant/Award Number: BM1402Peer reviewe

Karyotype and genome size of Iberochondrostoma almacai (Teleostei, Cyprinidae) and comparison with the sister-species I.lusitanicum

This study aimed to define the karyotype of the recently described Iberian endemic Iberochondrostoma almacai, to revisit the previously documented chromosome polymorphisms of its sister species I.lusitanicum using C-, Ag-/CMA3 and RE-banding, and to compare the two species genome sizes. A 2n = 50 karyotype (with the exception of a triploid I.lusitanicum specimen) and a corresponding haploid chromosome formula of 7M:15SM:3A (FN = 94) were found. Multiple NORs were observed in both species (in two submetacentric chromosome pairs, one of them clearly homologous) and a higher intra and interpopulational variability was evidenced in I.lusitanicum. Flow cytometry measurements of nuclear DNA content showed some significant differences in genome size both between and within species: the genome of I. almacai was smaller than that of I.lusitanicum (mean values 2.61 and 2.93 pg, respectively), which presented a clear interpopulational variability (mean values ranging from 2.72 to 3.00 pg). These data allowed the distinction of both taxa and confirmed the existence of two well differentiated groups within I. lusitanicum: one that includes the populations from the right bank of the Tejo and Samarra drainages, and another that reunites the southern populations. The peculiar differences between the two species, presently listed as “Critically Endangered”, reinforced the importance of this study for future conservation plans