Descripción del parasitismo gastrointestinal mediante análisis coprológico en zorro de Darwin, Lycalopex fulvipes (Martin 1837), y güiña, Leopardus guigna (Molina 1782), en isla de Chiloé, Chile

We aimed to identify faeces belonging to either Darwin’s fox (Lycalopex fulvipes) or kodkod (Leopardus guigna) by using molecular genetic techniques, and to describe and compare the gastrointestinal parasites harbored by both carnivores in Chiloé Island. We found that 60% (25/42) and 88% (28/32) of faeces of Darwin’s fox and kodkod had parasite eggs. This study provided the fi rst record of Aspiculuris sp. and trematodes in Darwin’s fox and Aspiculuris sp., Trichuris sp., Capillaria sp., Isospora sp. and trematodes in kodkod, which may correspond to either prey- or host-originated parasites.Nuestro objetivo fue identifi car heces pertenecientes a zorro de Darwin (Lycalopex fulvipes) o güiña (Leopardus guigna) utilizando técnicas de genética molecular, y describir y comparar los parásitos gastrointestinales albergados por ambos carnívoros en la isla de Chiloé. Encontramos que 60% (25/42) y 88% (28/32) de heces de zorro de Darwin y güiña tuvo huevos de parásito. Este estudio entregó el primer registro de Aspiculuris sp. y tremátodos en zorro de Darwin y Aspiculuris sp., Trichuris sp., Capillaria sp., Isospora sp. y tremátodos en güiña, que podrían corresponder a parásitos de presas u hospederos

Aplicación transversal de los contenidos curriculares de Matemática I de las carreras de Farmacia y Bioquímica: desarrollo de una nueva didáctica de la materia

Los contenidos de Matemática I se utilizan en un elevado porcentaje de las materias curriculares de 1º, 2º y primer semestre de 3º año de las carreras de Farmacia y Bioquímica de la Universidad Juan A. Maza, se pretende orientar la enseñanza de esta materia básica a partir de las necesidades de las disciplinares, propiciando el desarrollo de las competencias específicas que permitan a los alumnos aumentar el nivel de entendimiento y la intervinculación de los contenidos, a fin de mejorar su rendimiento académico. Este enfoque anticipativo, basado en ejemplos concretos que los alumnos enfrentarán en otras materias disciplinares de sus primeros años, brindará además motivación a la hora de aprender matemática, al contextualizar sus contenidos en temáticas propias de la carrera que los estudiantes eligieron y que hará el aprendizaje matemático más interesante

Interventions at the hospital “Torcuato de Alvear”:a multidiciplinary experience under a “ciudad total”

Entendiendo a la ciudad desde un concepto de “Ciudad Total” se buscó abordar las intervenciones realizadas en el Hospital Alvear de una manera multidisciplinaria. De esta forma, desde lo patrimonial, lo arqueológico, lo paleontológico y lo botánico se emprendieron diferentes tipos de acciones tendientes a diagnosticar el complejo hospitalario. Sus obras comenzaron en 1908 y tras más de cien años de existencia, requerían de su estudio para una puesta en valor del conjunto edilicio y diagnóstico de sus espacios verdes, en el sector donde funcionaran una serie de pabellones demolidos en la década de 1970.Compreendendo a cidade a partir de um conceito de “cidade total” procuramos abordar as intervenções realizadas no Hospital Alvear de forma multidisciplinar. Dessa maneira, desde o patrimonial, o arqueológico, o paleontológico e o botânico, diferentes ações serão desenvolvidas com tendencias para diagnosticar o complexo hospitalar. A construção começou em 1908, e depois de mais de cem anos de existencia, necesitava de um estudo para uma valorização do complexo de edificio e diagnostico das suas zonas verdes circundantes, que incluiran um levantamento do potencial arqueologico no setor onde funcionava uma serie de pavilhões demolidos na decada de 1970.Understanding the city from a concept of "Ciudad Total" (Comprehensive City) we sought to address the interventions made at the Alvear Hospital from a multidisciplinary point of view. In this sense, different types of actions were engaged on archaeology, paleontology and heritage tending to diagnose this hospital complex, whose building works started in 1908 and, after more than a hundred years of existence, was in the need of a study for an enhancement of the building complex and a diagnose of its surrounding green spaces, including a survey in order to detect archaeological potential in the sector where there used to be a group of wards that were demolished in the 1970s.Facultad de Ciencias Naturales y Muse

Transverse uses of the contents of Mathematics I in Pharmacy and Biochemistry courses of study. Development of a new didactics

Considerando que los contenidos de Matemática I se utilizan en un elevado porcentaje de las materias curriculares de primero, segundo y primer semestre de tercer año de las carreras de Farmacia y Bioquímica, se pretende orientar la enseñanza de esta materia básica a partir de las necesidades disciplinares, propiciando el desarrollo de las competencias específicas que permitan a los alumnos aumentar el nivel de entendimiento y la vinculación de los contenidos, a fin de mejorar su rendimiento

Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendations

The quantitation of BCR-ABL1 mRNA is mandatory for chronic myeloid leukemia (CML) patients, and RT-qPCR is the most extensively used method in testing laboratories worldwide. Nevertheless, substantial variation in RT-qPCR results makes inter-laboratory comparability hard. To facilitate inter-laboratory comparative assessment, an international scale (IS) for BCR-ABL1 was proposed.The laboratory-specific conversion factor (CF) to the IS can be derived from the World Health Organization (WHO) genetic reference panel; however, this material is limited to the manufacturers to produce and calibrate secondary reference reagents. Therefore, we developed secondary reference calibrators, as lyophilized cellular material, aligned to the IS. Our purpose was both to re-evaluate the CF in 18 previously harmonized laboratories and to propagate the IS to new laboratories.Results Our field trial including 30 laboratories across Latin America showed that, after correction of raw BCR-ABL1/ABL1 ratios using CF, the relative mean bias was significantly reduced. We also performed a follow-up of participating laboratories by annually revalidating the process; our results support the need for continuous revalidation of CFs. All participating laboratories also received a calibrator to determine the limit of quantification (LOQ); 90% of them could reproducibly detect BCR-ABL1, indicating that these laboratories can report a consistent deep molecular response. In addition, aiming to investigate the variability of BCR-ABL1 measurements across different RNA inputs, we calculated PCR efficiency for each individual assay by using different amounts of RNA.Conclusions In conclusion, for the first time in Latin America, we have successfully organized a harmonization platform for BCR-ABL1 measurement that could be of immediate clinical benefit for monitoring the molecular response of patients in low-resource regions.Fil: Ruiz, María Sol. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sánchez, María Belén. Fundación Cáncer. Centro de Investigaciones Oncológicas; ArgentinaFil: Vera Contreras, Yuly Masiel. Fundación Cáncer. Centro de Investigaciones Oncológicas; ArgentinaFil: Agrielo, Evangelina. Laboratorio Especialidades Bioquímicas; ArgentinaFil: Alonso, Marta. Hospital Nacional Profesor Alejandro Posadas; ArgentinaFil: Altuna, María Eugenia. Clinica Roberto Raña; ArgentinaFil: Anchordoqui, María Sol. Argenomics; ArgentinaFil: Asinari, Mariana. Hospital Privado Centro Médico de Córdoba; ArgentinaFil: Bonetto, María Elisa. Hospital Rawson San Juan; ArgentinaFil: Camargo, Mauricio. Laboratorio Medellin; ColombiaFil: Giere, Isabel. Fundación Para Combatir la Leucemia; ArgentinaFil: González, Javier. Meyer Lab; ParaguayFil: Granda Alacote, Ana Cecilia. Laboratorios Medicos Lima; PerúFil: Guerra, Javier. Nanopharmacia Diagnóstica Mexico; MéxicoFil: Gutiérrez, Marina. Stamboulian; ArgentinaFil: Maldonado, Cecilia. Manlab; ArgentinaFil: Makiya, Ricard. No especifíca;Fil: Manrique, Gonzalo. Asociacion Española de Montevideo; UruguayFil: Monaco, María Eugenia. Laboratorio Tucuman; ArgentinaFil: Rozo, Juan Carlos. Unidad de Diagnóstico Hemato Oncológico; ColombiaFil: Santamaría Martín, Carlos Jose. Hospital de Niños San Jose de Costa Rica; Costa Rica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Seravalle, Analía. Civic Rosario; ArgentinaFil: Zea, Olga. Laboratorio Medellin Colombia; ColombiaFil: Zubillaga, María Noel. Asociación Española de Socorros Mutuos; UruguayFil: Mordoh, José. Fundación Cáncer. Centro de Investigaciones Oncológicas; ArgentinaFil: Larripa, Irene Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Bianchini, Michele. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

FACTORES ANTROPOGÉNICOS YAMBIENTALES SOBRE LA FAUNA DE CULÍCIDOS (DIPTERA: CULICIDAE) DE LA PROVINCIA SANCTI SPÍRITUS, CUBA

El objetivo fue identificar las especies de mosquitos que habitan en los ecosistemas fluviales de la provincia Sancti Spíritus Cuba, así como los factores ambientales y antropogénicos que más influyen en la presencia y distribución de la fauna de culícidos en dicha provincia. Se realizaron seis muestreos en 90 ecosistemas fluviales de los ocho municipios que conforman la provincia entre el 2000 al 2011. Se identificaron 33 especies de mosquitos, donde los municipios de Trinidad, Sancti Spíritus, La Sierpe, Fomento y Yaguajay resultaron ser los de mayor riqueza de especies. Las mayores frecuencias o abundancias relativas resultaron ser para los géneros Culex (55,4%), Anopheles (22,2%), Mansonia (18,0%) y Ochlerotatus (10,2%). La presencia de las larvas de culícidos dependió en gran medida del tipo de hábitat, estrato contextual y abundancia de la vegetación, la cual fue más intensa en los ríos, en especial, la de tipo flotante; mientras que en las cañadas, zanjas y esteros, la vegetación fue escasa. La contaminación constituyó un factor limitante para el crecimiento y desarrollo, no solo de la vegetación, sino también de organismos del reino animal, incluidos los mosquitos. Si tenemos en cuenta la elevada riqueza de especies de culícidos de interés sanitario existente en la provincia y la alta incidencia que tienen los factores antropogénicos y ambientales analizados, es evidente el riesgo potencial que representa para la salud humana y animal de esta provincia la aparición de estas entidades infecciosas

Trends in the epidemiology of catheter-related bloodstream infections; towards a paradigm shift, Spain, 2007 to 2019

Altres ajuts: Departament de Salut. Generalitat de Catalunya ("Pla estratègic de recerca i innovació en salut (PERIS) 2019-2021"); Ministerio de Asuntos Económicos y Transformación Digital; Red Española de Investigación en Patología Infecciosa (REIPI).Background: Catheter-related bloodstream infections (CRBSI) are frequent healthcare-associated infections and an important cause of death. Aim: To analyse changes in CRBSI epidemiology observed by the Infection Control Catalan Programme (VINCat). Methods: A cohort study including all hospital-acquired CRBSI episodes diagnosed at 55 hospitals (2007-2019) in Catalonia, Spain, was prospectively conducted. CRBSI incidence rates were adjusted per 1,000patientdays. To assess the CRBSI rate trend per year, negative binomial models were used, with the number of events as the dependent variable, and the year as the main independent variable. From each model, the annual rate of CRBSI diagnosed per 1,000patientdays and the incidence rate ratio (IRR) with its 95% confidence intervals (CI) were reported. Results: During the study, 9,290 CRBSI episodes were diagnosed (mean annual incidence rate:0.20episodes/1,000patientdays). Patients' median age was 64.1years; 36.6% (3,403/9,290) were female. In total, 73.7% (n=6,845) of CRBSI occurred in non-intensive care unit (ICU) wards, 62.7% (n=5,822) were related to central venous catheter (CVC), 24.1% (n=2,236) to peripheral venous catheters (PVC) and 13.3% (n=1,232) to peripherally-inserted central venous catheters (PICVC). Incidence rate fell over the study period (IRR:0.94;95%CI:0.93-0.96), especially in the ICU (IRR:0.88;95%CI:0.87-0.89). As a whole, while episodes of CVC CRBSI fell significantly (IRR:0.88;95%CI:0.87-0.91), peripherally-inserted catheter CRBSI (PVC and PICVC) rose, especially in medical wards (IRR PICVC:1.08;95%CI:1.05-1.11; IRR PVC: 1.03; 95% 1.00-1.05). Conclusions: Over the study, CRBSIs associated with CVC and diagnosed in ICUs decreased while episodes in conventional wards involving peripherally-inserted catheters increased. Hospitals should implement preventive measures in conventional wards

Probing extreme environments with the Cherenkov Telescope Array

The physics of the non-thermal Universe provides information on the acceleration mechanisms in extreme environments, such as black holes and relativistic jets, neutron stars, supernovae or clusters of galaxies. In the presence of magnetic fields, particles can be accelerated towards relativistic energies. As a consequence, radiation along the entire electromagnetic spectrum can be observed, and extreme environments are also the most likely sources of multi-messenger emission. The most energetic part of the electromagnetic spectrum corresponds to the very-high-energy (VHE, E>100 GeV) gamma-ray regime, which can be extensively studied with ground based Imaging Atmospheric Cherenkov Telescopes (IACTs). The results obtained by the current generation of IACTs, such as H.E.S.S., MAGIC, and VERITAS, demonstrate the crucial importance of the VHE band in understanding the non-thermal emission of extreme environments in our Universe. In some objects, the energy output in gamma rays can even outshine the rest of the broadband spectrum. The Cherenkov Telescope Array (CTA) is the next generation of IACTs, which, with cutting edge technology and a strategic configuration of ~100 telescopes distributed in two observing sites, in the northern and southern hemispheres, will reach better sensitivity, angular and energy resolution, and broader energy coverage than currently operational IACTs. With CTA we can probe the most extreme environments and considerably boost our knowledge of the non-thermal Universe.Comment: Submitted as input to ASTRONET Science Vision and Infrastructure roadmap on behalf of the CTA consortiu

Detection of persistent VHE gamma-ray emission from PKS 1510-089 by the MAGIC telescopes during low states between 2012 and 2017

PKS 1510-089 is a flat spectrum radio quasar strongly variable in the optical and GeV range. To date, very high-energy (VHE, > 100 GeV) emission has been observed from this source either during long high states of optical and GeV activity or during short flares. Aims. We search for low-state VHE gamma-ray emission from PKS 1510-089. We characterize and model the source in a broadband context, which would provide a baseline over which high states and flares could be better understood. Methods. PKS 1510-089 has been monitored by the MAGIC telescopes since 2012. We use daily binned Fermi-LAT flux measurements of PKS 1510-089 to characterize the GeV emission and select the observation periods of MAGIC during low state of activity. For the selected times we compute the average radio, IR, optical, UV, X-ray, and gamma-ray emission to construct a low-state spectral energy distribution of the source. The broadband emission is modeled within an external Compton scenario with a stationary emission region through which plasma and magnetic fields are flowing. We also perform the emission-model-independent calculations of the maximum absorption in the broad line region (BLR) using two different models. Results. The MAGIC telescopes collected 75 hr of data during times when the Fermi-LAT flux measured above 1 GeV was below 3? × 10 -8 ? cm -2 ? s -1 , which is the threshold adopted for the definition of a low gamma-ray activity state. The data show a strongly significant (9.5¿) VHE gamma-ray emission at the level of (4.27 ± 0.61 stat ) × 10 -12 ? cm -2 ? s -1 above 150 GeV, a factor of 80 lower than the highest flare observed so far from this object. Despite the lower flux, the spectral shape is consistent with earlier detections in the VHE band. The broadband emission is compatible with the external Compton scenario assuming a large emission region located beyond the BLR. For the first time the gamma-ray data allow us to place a limit on the location of the emission region during a low gamma-ray state of a FSRQ. For the used model of the BLR, the 95% confidence level on the location of the emission region allows us to place it at a distance > 74% of the outer radius of the BLR. © ESO 2018.The financial support of the German BMBF and MPG, the Italian INFN and INAF, the Swiss National Fund SNF, the ERDF under the Spanish MINECO (FPA2015-69818-P, FPA2012-36668, FPA2015-68378-P, FPA2015-69210-C6-2-R, FPA2015-69210-C6-4-R, FPA2015-69210-C6-6-R, AYA2015-71042-P, AYA2016-76012-C3-1-P, ESP2015-71662-C2-2-P, CSD2009-00064), and the Japanese JSPS and MEXT is gratefully acknowledged. This work was also supported by the Spanish Centro de Exce-lencia “Severo Ochoa” SEV-2012-0234 and SEV-2015-0548, and Unidad de Excelencia “María de Maeztu” MDM-2014-0369, by the Croatian Science Foundation (HrZZ) Project IP-2016-06-9782 and the University of Rijeka Project 13.12.1.3.02, by the DFG Collaborative Research Centers SFB823/C4 and SFB876/C3, the Polish National Research Centre grant UMO-2016/22/M/ST9/00382, and by the Brazilian MCTIC, CNPq and FAPERJ. IA acknowledges support from a Ramón y Cajal grant of the Ministerio de Economía, Industria, y Competitividad (MINECO) of Spain. Acquisition and reduction of the POLAMI and MAPCAT data was supported in part by MINECO through grants AYA2010-14844, AYA2013-40825-P, and AYA2016-80889-P, and by the Regional Government of Andalucía through grant P09-FQM-4784.Peer Reviewe