The Effect of Inquiry Learning on the Academic Achievement and Bilingual Verbal Cognition of Young Bilingual Students

The issues that prompt this study are based on current research indicating the positive effects of inquiry learning on the cognitive development of children. The purpose of this case study was to understand the effects of inquiry learning on the academic achievement and bilingual verbal cognition of 5th grade bilingual students in a French/English dual immersion program. The treatment group of students completed research projects through a guided inquiry learning approach, while the control group experienced the traditional problem-solving research approach. Empirical findings reported a significant mean increase in mathematics achievement, bilingual verbal cognitive ability, higher motivation to learn and increased self-efficacy in the treatment versus the control group of students

PiggyBac-ing on a Primate Genome: Novel Elements, Recent Activity and Horizontal Transfer

To better understand the extent of Class II transposable element activity in mammals, we investigated the mouse lemur, Microcebus murinus, whole genome shotgun (2X) draft assembly. Analysis of this strepsirrhine primate extended previous research that targeted anthropoid primates and found no activity within the last 37 Myr. We tested the hypothesis that members of the piggyBac Class II superfamily have been inactive in the strepsirrhine lineage of primates during the same period. Evidence against this hypothesis was discovered in the form of three nonautonomous piggyBac elements with activity periods within the past 40 Myr and possibly into the very recent past. In addition, a novel family of piggyBac transposons was identified, suggesting introduction via horizontal transfer. A second autonomous element was also found with high similarity to an element recently described from the little brown bat, Myotis lucifugus, further implicating horizontal transfer in the evolution of this genome. These findings indicate a more complex history of transposon activity in mammals rather than a uniform shutdown of Class II transposition, which had been suggested by analyses of more common model organisms

KINEMATIC ANALYSIS OF MEN BOBSLED PUSH STARTS

The purpose of this study was to provide a descriptive analysis of selected kinematic variables associated with the push start for brakemen in the two-man bobsled. Eleven male bobsledders served as subjects for this study. The subjects were videotaped at 120 Hz during competition at the Lake Placid, NY bobsled track as they were competing for positions on the men’s 2004 National Bobsled Team. Selected groups of variables measured at specific events in this study included step length, frequency, and foot contact time; COM velocity; and trunk, knee, and elbow angles. A relationship of 0.63 was determined between start time and finish time; an exploratory correlational analysis between start time and COM velocity at 2nd step take-off (TO) was -0.63. Start time is of critical importance in determining final race time

Potency of a human monoclonal antibody to diphtheria toxin relative to equine diphtheria anti-toxin in a guinea pig intoxication model

Prompt administration of anti-toxin reduces mortality following Corynebacterium diphtheriae infection. Current treatment relies upon equine diphtheria anti-toxin (DAT), with a 10% risk of serum sickness and rarely anaphylaxis. The global DAT supply is extremely limited; most manufacturers have ceased production. S315 is a neutralizing human IgG1 monoclonal antibody to diphtheria toxin that may provide a safe and effective alternative to equine DAT and address critical supply issues. To guide dose selection for IND-enabling pharmacology and toxicology studies, we dose-ranged S315 and DAT in a guinea pig model of diphtheria intoxication based on the NIH Minimum Requirements potency assay. Animals received a single injection of antibody premixed with toxin, were monitored for 30 days, and assigned a numeric score for clinical signs of disease. Animals receiving \u3e /= 27.5 microg of S315 or \u3e /= 1.75 IU of DAT survived whereas animals receiving \u3c /= 22.5 microg of S315 or \u3c /= 1.25 IU of DAT died, yielding a potency estimate of 17 microg S315/IU DAT (95% CI 16-21) for an endpoint of survival. Because some surviving animals exhibited transient limb weakness, likely a systemic sign of toxicity, DAT and S315 doses required to prevent hind limb paralysis were also determined, yielding a relative potency of 48 microg/IU (95% CI 38-59) for this alternate endpoint. To support advancement of S315 into clinical trials, potency estimates will be used to evaluate the efficacy of S315 versus DAT in an animal model with antibody administration after toxin exposure, more closely modeling anti-toxin therapy in humans

A Qualitative Study of the Motivations and Affiliation Dynamics Involved with a Firefighting Career

We explored the experiences of full-time firefighters in the present phenomenological qualitative study, having conducted semi-structured interviews with 26 male full-time firefighters. Their personal constructs of motivation and affiliation were explored and, within the constructs of motivation, three themes emerged. First, firefighters were motivated by a love of the excitement firefighting provides. Second, firefighters reported that the work schedule, which allows them more time at home, was a motivation. Third (and most emphasized by the firefighters) was an altruistic motivation to help others. Under the construct of affiliation, the firefighters reported a strong sense of brotherhood with their shift-partners, and they extended this brotherhood to all firefighters and even other emergency workers. We relate these findings to the existing body of research regarding the relationship between motivation, affiliation and satisfaction of firefighters

Producing facial composite sketches in remote cognitive interviews:a preliminary investigation

Justice systems around the world are increasingly turning to videoconferencing as a means to reduce delays and reduce costs in legal processes. This preliminary research examined whether interviewing a witness remotely - without physical co-presence of the witness and interviewer - could facilitate the production of quality facial composite sketches of suspects. In Study 1, 42 adults briefly viewed a photograph of a face. The next day they participated in Cognitive Interviews with a forensic artist, conducted either face-to-face or remotely via videoconference. In Study 2, 20 adults participated in videoconferenced interviews, and we manipulated the method by which they viewed the developing sketch. In both studies, independent groups of volunteers rated the likeness of the composites to the original photographs. The data suggest that remote interviews elicited effective composites; however, in Study 1 these composites were considered poorer matches to the photographs than were those produced in face-to-face interviews. The differences were small, but significant. Participants perceived several disadvantages to remote interviewing, but also several advantages including less pressure and better concentration. The results of Study 2 suggested that different sketch presentation methods offered different benefits. We propose that remote interviewing could be a useful tool for investigators in certain circumstances

Data Recovery Excavations of the Carriage House, Greenhouse, and Greenhouse/Carriage House Well at Gore Place, Waltham, Massachusetts

Excavations and ground penetrating radar at Gore Place in Waltham, Massachusetts, examined the original 1793 carriage house site, the 1806 greenhouse, and the greenhouse/carriage house well, all associated with Christopher and Rebecca Gore. The carriage house was moved in 1968, and its cellar was filled at that time. Mechanical removal of the fill in a portion of the carriage house cellar revealed that the lower portion of at least the rear (north) foundation wall is well preserved along with the cellar floor. Documentary evidence indicated that the carriage house cellar had been used for manure (compost) preparation, while the first floor was used to house horses and to store gardening tools and firewood. Four excavation units set into the cellar floor revealed no evidence of its former use for manure production (such as organic staining) indicating that it had been thoroughly cleaned. The artifacts present in the floor units represent a considerable time period from that of the Gores through to the early 20th century. The majority of objects date to the Gore period, and the wide variety may reflect the incorporation of refuse into manure production. Investigations north of the structure showed that some of the soil in the parking lot constituting the western portion of Gore’s vegetable garden was removed and replaced with a uniform mixture of sand and gravel, probably at the time the carriage house was moved. While the gravel is at least 2 m deep close to the carriage house foundation, its depth lessens with distance northward, since shovel testing in 2004 showed intact dark brown loamy soil beginning at a depth of 35 to 50 cm below driveway gravel and sand bedding at 20 and 40 m north of the carriage house foundation. Early 19th-century maps indicated that the greenhouse was roughly 60 feet (18 m) long and 15 to 21 feet (up to 6.5 m) wide with a small extension on the west end. Fifty-two square meters were excavated at the west end, uncovering the trapezoidal brick fl oor of the extension and an associated stone drain, ground surfaces contemporary with the greenhouse, post holes for a fence that separated the greenhouse area from the carriage house, layers relating to the greenhouse’s destruction (early 1840s), and later landscaping features including a stone wall and two drains. Documentary, archaeological, and geophysical data suggest that the greenhouse was a formal space intended to grow and display exotic plants and that it was built in the relatively new lean-to style, with a tall back wall and short front wall. The artifact assemblage included architectural elements, tools and small finds related to the greenhouse operation (including the remains of at least 149 planting pots), and bone stockpiled for soil enrichment. The greenhouse was constructed by the Gores during a period of intense interest in agricultural experimentation by members of the Massachusetts commercial and political elite. Scholars have argued that these men used the positive associations of agriculture to offset some of the contemporary negative connotations of commerce. This report examines the greenhouse both as a space for the display of exotic plants in the context of this scientifi c agricultural movement and posits that Rebecca Gore may have played a signifi cant role in managing it

Investigating a genetic link between Alzheimer’s Disease and CADASIL related Cerebral Small Vessel Disease

Monogenic forms of Alzheimer’s disease (AD) have been identified through mutations in genes such as APP, PSEN1, and PSEN2, whilst other genetic markers such as the APOE ε carrier allele status have been shown to increase the likelihood of having the disease. Mutations in these genes are not limited to AD, as APP mutations can also cause an amyloid form of cerebral small vessel disease (CSVD) known as cerebral amyloid angiopathy, whilst PSEN1 and PSEN2 are involved in NOTCH3 signalling, a process known to be dysregulated in the monogenic CSVD, cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The overlap between AD genes and causes of CSVD led to the hypothesis that mutations in other genes within the PANTHER AD–presenilin pathway may be novel causes of CSVD in a cohort of clinically suspicious CADASIL patients without a pathogenic NOTCH3 mutation. To investigate this, whole exome sequencing was performed on 50 suspected CADASIL patients with no NOTCH3 mutations, and a targeted gene analysis was completed on the PANTHER. ERN1 was identified as a novel candidate CSVD gene following predicted pathogenic gene mutation analysis. Rare variant burden testing failed to identify an association with any gene; however, it did show a nominally significant link with ERN1 and TRPC3. This study provides evidence to support a genetic overlap between CSVD and Alzheimer’s disease.</p

Algal amendment enhances biogenic methane production from coals of different thermal maturity

The addition of small amounts of algal biomass to stimulate methane production in coal seams is a promising low carbon renewable coalbed methane enhancement technique. However, little is known about how the addition of algal biomass amendment affects methane production from coals of different thermal maturity. Here, we show that biogenic methane can be produced from five coals ranging in rank from lignite to low-volatile bituminous using a coal-derived microbial consortium in batch microcosms with and without algal amendment. The addition of 0.1 g/l algal biomass resulted in maximum methane production rates up to 37 days earlier and decreased the time required to reach maximum methane production by 17–19 days when compared to unamended, analogous microcosms. Cumulative methane production and methane production rate were generally highest in low rank, subbituminous coals, but no clear association between increasing vitrinite reflectance and decreasing methane production could be determined. Microbial community analysis revealed that archaeal populations were correlated with methane production rate (p = 0.01), vitrinite reflectance (p = 0.03), percent volatile matter (p = 0.03), and fixed carbon (p = 0.02), all of which are related to coal rank and composition. Sequences indicative of the acetoclastic methanogenic genus Methanosaeta dominated low rank coal microcosms. Amended treatments that had increased methane production relative to unamended analogs had high relative abundances of the hydrogenotrophic methanogenic genus Methanobacterium and the bacterial family Pseudomonadaceae. These results suggest that algal amendment may shift coal-derived microbial communities towards coal-degrading bacteria and CO2- reducing methanogens. These results have broad implications for understanding subsurface carbon cycling in coal beds and the adoption of low carbon renewable microbially enhanced coalbed methane techniques across a diverse range of coal geology