From Thermopylae To Leuctra: The Evolution of The Spartan Military Ethos

This paper examines the transformation of the Spartan military tradition from the Persian Wars to the Theban Ascendency. The Battle of Thermopylae marked the height of the Spartan military ethos. However, the strain of their military culture forced the Spartans to dilute their old mores over time. Their old cultural and military practices became unsustainable as military innovation increased. The Battle of Sphacteria clearly illustrated the deterioration of Spartan military ethos and the need for innovation. Straddling the line between tradition and innovation, Spartan military ethos received its death blow at the Battle of Leuctra

Conformational switching in the coiled-coil domains of a proteasomal ATPase regulates substrate processing

Protein degradation in all domains of life requires ATPases that unfold and inject proteins into compartmentalized proteolytic chambers. Proteasomal ATPases in eukaryotes and archaea contain poorly understood N-terminally conserved coiled-coil domains. In this study, we engineer disulfide crosslinks in the coiled-coils of the archaeal proteasomal ATPase (PAN) and report that its three identical coiled-coil domains can adopt three different conforma- tions: (1) in-register and zipped, (2) in-register and partially unzipped, and (3) out-of-register. This conformational heterogeneity conflicts with PAN’s symmetrical OB-coiled-coil crystal structure but resembles the conformational heterogeneity of the 26S proteasomal ATPases’ coiled-coils. Furthermore, we find that one coiled-coil can be conformationally constrained even while unfolding substrates, and conformational changes in two of the coiled-coils reg- ulate PAN switching between resting and active states. This switching functionally mimics similar states proposed for the 26S proteasome from cryo-EM. These findings thus build a mechanistic framework to understand regulation of proteasome activity

Micro electro mechanical cantilever with electrostatically controlled tip contact

A micro-electro-mechanical system (MEMS) cantilever that lifts from the surface by electrostatic force is described. The design is composed of three conductors: a fixed buried plate, a fixed surface plate, and a moveable cantilever. All have the same square shape and are arranged parallel in a vertical stack with aligned edges. The surface plate and cantilever are biased at the same potential, and the buried plate is oppositely biased. Theoretical analysis based on values of position-dependent coefficients of capacitance and electrostatic induction from finite element method demonstrates the sign of the force on the cantilever and determines its magnitude. Video microscopy and electrical measurements demonstrate the electrostatic lifting of the cantilever in a fabricated MEMS device. The vertical displacement of the cantilever is quantified from changes in optical interference fringes, and the displacement magnitude agrees with expectations based on estimated strengths of upward electrostatic force and downward elastic restoring force

C4 Operations Optimization

The primary objective of the project chronicled in this report was to design a model that optimizes C4 operations. This model will optimize the options for processing both “crude C4” and “Cat BB” streams, taking the feed stream makeup, market pricings, and capacity constraints into account. Crude C4 streams contain butanes, butenes, and butadienes, while “Cat BB” streams are similar in makeup but do not include butadienes. It is assumed that the equipment for all unit operations is readily available. In addition, the plant will already have a baseload of feedstock that does not fully utilize all the equipment in the plant, which allows for the purchase of feedstock that could potentially be profitable. Available unit operations include Butadiene extraction, MTBE production, Metathesis, 1-Butene distillation, Skeletal Isomerization, Olefin Isomerization, and Alkylation. The model will be required to make choices about which unit operations to utilize based on the constraints input by the user. While the model will not directly produce revenue, it will allow the company to optimize processes within the plant, finding the most profitable situation. Thus, it will be possible to assess the program’s value based on its accuracy

Brain hubs defined in the group do not overlap with regions of high inter-individual variability

Connector \u27hubs\u27 are brain regions with links to multiple networks. These regions are hypothesized to play a critical role in brain function. While hubs are often identified based on group-average functional magnetic resonance imaging (fMRI) data, there is considerable inter-subject variation in the functional connectivity profiles of the brain, especially in association regions where hubs tend to be located. Here we investigated how group hubs are related to locations of inter-individual variability. To answer this question, we examined inter-individual variation at group-level hubs in both the Midnight Scan Club and Human Connectome Project datasets. The top group hubs defined based on the participation coefficient did not overlap strongly with the most prominent regions of inter-individual variation (termed \u27variants\u27 in prior work). These hubs have relatively strong similarity across participants and consistent cross-network profiles, similar to what was seen for many other areas of cortex. Consistency across participants was further improved when these hubs were allowed to shift slightly in local position. Thus, our results demonstrate that the top group hubs defined with the participation coefficient are generally consistent across people, suggesting they may represent conserved cross-network bridges. More caution is warranted with alternative hub measures, such as community density (which are based on spatial proximity to network borders) and intermediate hub regions which show higher correspondence to locations of individual variability

The relationship between cognitive ability and BOLD activation across sleep–wake states

The sleep spindle, a waxing and waning oscillation in the sigma frequency range, has been shown to correlate with fluid intelligence; i.e. the ability to use logic, learn novel rules/patterns, and solve problems. Using simultaneous EEG and fMRI, we previously identified the neural correlates of this relationship, including activation of the thalamus, bilateral putamen, medial frontal gyrus, middle cingulate cortex, and precuneus. However, research to date has focussed primarily on non-rapid eye movement (NREM) sleep, and spindles per se, thus overlooking the possibility that brain activity that occurs in other sleep–wake states might also be related to cognitive abilities. In our current study, we sought to investigate whether brain activity across sleep/wake states is also related to human intelligence in N = 29 participants. During NREM sleep, positive correlations were observed between fluid intelligence and blood oxygen level dependent (BOLD) activations in the bilateral putamen and the paracentral lobule/precuneus, as well as between short-term memory (STM) abilities and activity in the medial frontal cortex and inferior frontal gyrus. During wake, activity in bilateral postcentral gyri and occipital lobe was positively correlated with short-term memory abilities. In participants who experienced REM sleep in the scanner, fluid intelligence was positively associated with midbrain activation, and verbal intelligence was associated with right postcentral gyrus activation. These findings provide evidence that the relationship between sleep and intellectual abilities exists beyond sleep spindles

A new deep-depletion CCD for the red channel of the Palomar Double Spectrograph

The red channel of the Palomar Double Spectrograph (DBSP) on the 200-inch Hale Telescope has been upgraded with a new deep-depletion CCD from LBNL. Its redder response produced a significant increase of the throughput above 550 nm, and its longer dimension more than doubled the spectral coverage. A special Dewar was designed to accommodate a detector mount which includes features to minimize CCD motion due to thermal cycling, in spite of the very simple "picture frame" packaging of the CCD. The new Dewar also includes some novel features to improve the liquid nitrogen hold time while staying within the size envelope allowed in the Cassegrain cage. We describe these changes along with the detector characterization

Quasars: What turns them off?

(Abridged) We explore the idea that the anti-hierarchical turn-off observed in the quasar population arises from self-regulating feedback, via an outflow mechanism. Using a detailed hydrodynamic simulation we calculate the luminosity function of quasars down to a redshift of z=1 in a large, cosmologically representative volume. Outflows are included explicitly by tracking halo mergers and driving shocks into the surrounding intergalactic medium. Our results are in excellent agreement with measurements of the spatial distribution of quasars, and we detect an intriguing excess of galaxy-quasar pairs at very short separations. We also reproduce the anti-hierarchical turnoff in the quasar luminosity function, however, the magnitude of the turn-off falls short of that observed as well as that predicted by analogous semi-analytic models. The difference can be traced to the treatment of gas heating within galaxies. The simulated galaxy cluster L_X-T relationship is close to that observed for z~1 clusters, but the simulated galaxy groups at z=1 are significantly perturbed by quasar outflows, suggesting that measurements of X-ray emission in high-redshift groups could well be a "smoking gun" for the AGN heating hypothesis.Comment: 16 pages, 11 figures, submitted to ApJ, comments welcome

Generating intravital super-resolution movies with conventional microscopy reveals actin dynamics that construct pioneer axons

Super-resolution microscopy is broadening our in-depth understanding of cellular structure. However, super-resolution approaches are limited, for numerous reasons, from utilization in longer-term intravital imaging. We devised a combinatorial imaging technique that combines deconvolution with stepwise optical saturation microscopy (DeSOS) to circumvent this issue and image cells in their native physiological environment. Other than a traditional confocal or two-photon microscope, this approach requires no additional hardware. Here, we provide an open-access application to obtain DeSOS images from conventional microscope images obtained at low excitation powers. We show that DeSOS can be used in time-lapse imaging to generate super-resolution movies in zebrafish. DeSOS was also validated in live mice. These movies uncover that actin structures dynamically remodel to produce a single pioneer axon in a 'top-down' scaffolding event. Further, we identify an F-actin population - stable base clusters - that orchestrate that scaffolding event. We then identify that activation of Rac1 in pioneer axons destabilizes stable base clusters and disrupts pioneer axon formation. The ease of acquisition and processing with this approach provides a universal technique for biologists to answer questions in living animals