Flexoelectric polarisation effects in nematic liquid crystal phase gratings

Nematic phase gratings have been studied in which a planar nematic layer of thickness 17.2 μm is sandwiched between two glass substrates coated with an alignment polymer. The upper substrate is a continuous earth plane and the lower substrate has a patterned electrode of interdigitated stripes (electrodes and gaps are both 40 μm wide). Reorientation of the nematic liquid crystal occurs in response to d.c. electric fields applied between the interdigitated electrodes. These nematic reorientation regions have been used to investigate the influence of the flexoelectric polarisation in the nematic liquid crystal by observing the resultant (i) movement of tilt fringes in a Mach-Zehnder interferometer, and (ii) optical diffraction patterns. In the Mach-Zehnder interferometer the periodic variation of the refractive index resulting from the periodic distortion profile is measured directly from the displacement of the tilt fringes. The asymmetry in the response to positive and negative polarities of the d.c. voltage for both measurement techniques is directly related to the sum of the flexoelectric coefficients, e1 + e3

Comparative genomics of Aeschynomene symbionts : insights into the ecological lifestyle of nod-independent photosynthetic Bradyrhizobia

Tropical aquatic species of the legume genus Aeschynomene are stem- and root-nodulated by bradyrhizobia strains that exhibit atypical features such as photosynthetic capacities or the use of a nod gene-dependent (ND) or a nod gene-independent (NI) pathway to enter into symbiosis with legumes. In this study we used a comparative genomics approach on nine Aeschynomene symbionts representative of their phylogenetic diversity. We produced draft genomes of bradyrhizobial strains representing different phenotypes: five NI photosynthetic strains (STM3809, ORS375, STM3847, STM4509 and STM4523) in addition to the previously sequenced ORS278 and BTAi1 genomes, one photosynthetic strain ORS285 hosting both ND and NI symbiotic systems, and one NI non-photosynthetic strain (STM3843). Comparative genomics allowed us to infer the core, pan and dispensable genomes of Aeschynomene bradyrhizobia, and to detect specific genes and their location in Genomic Islands (GI). Specific gene sets linked to photosynthetic and NI/ND abilities were identified, and are currently being studied in functional analyses

Asymptotic solutions for an electrically induced Freedericksz transition in a wedge of smectic C liquid crystal

Theoretical work based on the Freedericksz transition in a wedge of smectic C liquid crystal is presented. Continuum theory is employed in order to mathematically model the two-way interaction between the anisotropic fluid and an applied electric field. Asymptotic methods are used to obtain concise and informative explicit solutions for limiting regimes where (a) the applied voltage is just above threshold, and (b) a high voltage is applied. As is anticipated, in the case of a small dielectric anisotropy, the solution reduces to that obtained when the two-way interaction is neglected. Nevertheless, at voltages close to threshold, this interaction can have a significant effect upon the director profile. Realistic material, geometry and field parameters are adopted in order to display these solutions. By comparing them with those obtained using a numerical method, a high degree of accuracy can be found within the above regimes

Interferometric method for the measurement of the flexoelectric polarisation using nematic liquid crystal phase gratings

An interferometric method using a nematic liquid crystal phase grating has recently been developed for determining the sum of the flexoelectric coefficients, (e1 + e3) [C. Trabi, C. V. Brown, A. A. T. Smith and N. J. Mottram, Appl. Phys. Lett. 92, 223509 (2008)]. The switching response of the device can be simulated if the applied 0.2 Hz squarewave voltage in the nematic layer is modified so that it is enhanced by a reverse ion-field after each polarity reversal and then decays with time. This process has been investigated using measurements of the transient capacitance of a planar nematic layer to the same waveform

The transcriptomic response of the murine thyroid gland to iodide overload and the role of the nrf2 antioxidant system

Background: Thyroid follicular cells have physiologically high levels of reactive oxygen species because oxidation of iodide is essential for the iodination of thyroglobulin (Tg) during thyroid hormone synthesis. Thyroid follicles (the functional units of the thyroid) also utilize incompletely understood autoregulatory mechanisms to defend against exposure to excess iodide. To date, no transcriptomic studies have investigated these phenomena in vivo. Nuclear erythroid factor 2 like 2 (Nrf2 or Nfe2l2) is a transcription factor that regulates the expression of numerous antioxidant and other cytoprotective genes. We showed previously that the Nrf2 pathway regulates the antioxidant defense of follicular cells, as well as Tg transcription and Tg iodination. We, thus, hypothesized that Nrf2 might be involved in the transcriptional response to iodide overload. Methods: C57BL6/J wild-type (WT) or Nrf2 knockout (KO) male mice were administered regular water or water supplemented with 0.05% sodium iodide for seven days. RNA from their thyroids was prepared for next-generation RNA sequencing (RNA-Seq). Gene expression changes were assessed and pathway analyses were performed on the sets of differentially expressed genes. Results: Analysis of differentially expressed messenger RNAs (mRNAs) indicated that iodide overload upregulates inflammatory-, immune-, fibrosis-and oxidative stress-related pathways, including the Nrf2 pathway. Nrf2 KO mice showed a more pronounced inflammatory–autoimmune transcriptional response to iodide than WT mice. Compared to previously published datasets, the response patterns observed in WT mice had strong similarities with the patterns typical of Graves’ disease and papillary thyroid carcinoma (PTC). Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) also responded to iodide overload, with the latter targeting mRNAs that participate mainly in inflammation pathways. Conclusions: Iodide overload induces the Nrf2 cytoprotective response and upregulates inflammatory, immune, and fibrosis pathways similar to autoimmune hyperthyroidism (Graves’ disease) and PTC. © 2020 by the authors. Licensee MDPI, Basel, Switzerland

Early life infection and proinflammatory, atherogenic metabolomic and lipidomic profiles in infancy: a population-based cohort study

BACKGROUND: The risk of adult onset cardiovascular and metabolic (cardiometabolic) disease accrues from early life. Infection is ubiquitous in infancy and induces inflammation, a key cardiometabolic risk factor, but the relationship between infection, inflammation, and metabolic profiles in early childhood remains unexplored. We investigated relationships between infection and plasma metabolomic and lipidomic profiles at age 6 and 12 months, and mediation of these associations by inflammation. METHODS: Matched infection, metabolomics, and lipidomics data were generated from 555 infants in a pre-birth longitudinal cohort. Infection data from birth to 12 months were parent-reported (total infections at age 1, 3, 6, 9, and 12 months), inflammation markers (high-sensitivity C-reactive protein [hsCRP]; glycoprotein acetyls [GlycA]) were quantified at 12 months. Metabolic profiles were 12-month plasma nuclear magnetic resonance metabolomics (228 metabolites) and liquid chromatography/mass spectrometry lipidomics (776 lipids). Associations were evaluated with multivariable linear regression models. In secondary analyses, corresponding inflammation and metabolic data from birth (serum) and 6-month (plasma) time points were used. RESULTS: At 12 months, more frequent infant infections were associated with adverse metabolomic (elevated inflammation markers, triglycerides and phenylalanine, and lower high-density lipoprotein [HDL] cholesterol and apolipoprotein A1) and lipidomic profiles (elevated phosphatidylethanolamines and lower trihexosylceramides, dehydrocholesteryl esters, and plasmalogens). Similar, more marked, profiles were observed with higher GlycA, but not hsCRP. GlycA mediated a substantial proportion of the relationship between infection and metabolome/lipidome, with hsCRP generally mediating a lower proportion. Analogous relationships were observed between infection and 6-month inflammation, HDL cholesterol, and apolipoprotein A1. CONCLUSIONS: Infants with a greater infection burden in the first year of life had proinflammatory and proatherogenic plasma metabolomic/lipidomic profiles at 12 months of age that in adults are indicative of heightened risk of cardiovascular disease, obesity, and type 2 diabetes. These findings suggest potentially modifiable pathways linking early life infection and inflammation with subsequent cardiometabolic risk. FUNDING: The establishment work and infrastructure for the BIS was provided by the Murdoch Children's Research Institute (MCRI), Deakin University, and Barwon Health. Subsequent funding was secured from National Health and Medical Research Council of Australia (NHMRC), The Shepherd Foundation, The Jack Brockhoff Foundation, the Scobie & Claire McKinnon Trust, the Shane O'Brien Memorial Asthma Foundation, the Our Women's Our Children's Fund Raising Committee Barwon Health, the Rotary Club of Geelong, the Minderoo Foundation, the Ilhan Food Allergy Foundation, GMHBA, Vanguard Investments Australia Ltd, and the Percy Baxter Charitable Trust, Perpetual Trustees. In-kind support was provided by the Cotton On Foundation and CreativeForce. The study sponsors were not involved in the collection, analysis, and interpretation of data; writing of the report; or the decision to submit the report for publication. Research at MCRI is supported by the Victorian Government's Operational Infrastructure Support Program. This work was also supported by NHMRC Senior Research Fellowships to ALP (1008396); DB (1064629); and RS (1045161) , NHMRC Investigator Grants to ALP (1110200) and DB (1175744), NHMRC-A*STAR project grant (1149047). TM is supported by an MCRI ECR Fellowship. SB is supported by the Dutch Research Council (452173113)

Population-based plasma lipidomics reveals developmental changes in metabolism and signatures of obesity risk: a mother-offspring cohort study

10.1186/s12916-022-02432-yBMC Medicine20124