Skip the Pre-Concert Demo: How Technical Familiarity and Musical Style Affect Audience Response

This paper explores the roles of technical and musical familiarity in shaping audience response to digital musical instrument (DMI) performances. In an audience study conducted during an evening concert, we examined two primary questions: first, whether a deeper understanding of how a DMI works increases an audience’s enjoyment and interest in the performance; and second, given the same DMI and same performer, whether playing in a conventional (vernacular) versus an experimental musical style affects an audience’s response. We held a concert in which two DMI creator-performers each played two pieces in differing styles. Before the concert, each half the 64-person audience was given a technical explanation of one of the instruments. Results showed that receiving an explanation increased the reported understanding of that instrument, but had no effect on either the reported level of interest or enjoyment. On the other hand, performances in experimental versus conventional style on the same instrument received widely divergent audience responses. We discuss implications of these findings for DMI design

Hands where we can see them! Investigating the impact of gesture size on audience perception

This paper explores the relative effect of gesture size on audience perception of digital musical instrument (DMI) performance. In a study involving a total audience of 28 people (split into 2 groups of 13 and 15), we used a small and large version of a DMI to examine how the size of performers' gestures might differ, and how this affects post-hoc audience ratings of enjoyment, interest and understanding, as well as their indications of `enjoyment' and `error' in real time. For each audience we held two 5-minute performances, the first on a custom-designed percussion DMI, and the second on a laptop. The DMI used in each performance was made up of three elements identical in shape, materiality, interaction and sound, but the physical size was different: For one each element was approx 12x10x5cm, and the other was about 3.5 times bigger (approx. 40x30x20cm). Data was collected both during and after the performance via post-hoc and real-time methods. We found that beyond a performance simply involving physical gesture, the size of gesture has an impact on audience ratings. In this paper we detail this study and its results, and present the implications that this finding has for DMI design

Assessing the impact of temporary housing sites on urban socio-spatial performance: the case of the Central Italy earthquake

This paperadvocatesa performance-based approach to the planningof temporary housing sites after sudden urban disasters. A “functionally graded”configurational analysis method is usedto assess,quantitatively and qualita-tively,thesocio-spatial impact of government-led housing assistance provisionacrossthe regional,urbanandneighbourhoodscales. To highlight the differentoutcomes achieved in different urban contextsby apparently similar housing re-covery plans, adiachronic comparative studyof four epicentral historic townshit by the 2016-2017Central Italyearthquakesis performed.The researchanalysestheconfigurationalpropertiesof these settlements at four critical points in time: before the disaster; right after it(emergency phase); during disaster recovery; after the reconstruction is completed. This paper builds on previous research on rapid urban modelling and economic spatial analysis workflows to respond to potential implementationchallenges,which include time constraints and geo-data availabilityissues after disasters. By using a real case scenario,this study seeks to demonstratethe potential benefitsof adopting the proposed multidimensional spatialanalysis methodto foster the delivery of integrated housing recovery so-lutions, which contribute to sustainable urban developmentTheseencompassin-forming, timely updating,and coordinatingstrategic, management,and opera-tional decisions related to the design and planningof temporary housing sites

Prediction of anti-Alzheimer’s activity of flavonoids targeting acetylcholinesterase in silico

Introduction – Prenylated and pyrano-flavonoids of the genus Artocarpus J. R. Forster & G. Forster are well known for their acetylcholinesterase (AchE) inhibitory, anticholinergic, antiinflammatory, antimicrobial, antioxidant, antiproliferative and tyrosinase inhibitory activities. Some of these compounds have also been shown to be effective against Alzheimer’s disease. Objective – The aim of the in silico study was to establish protocols to predict the most effective flavonoid from prenylated and pyrano-flavonoid classes for AchE inhibition linking to the potential treatment of Alzheimer’s disease. Methodology – Three flavonoids isolated from Artocarpus anisophyllus Miq. were selected for the study. With these compounds, Lipinski filter, ADME/Tox screening, molecular docking and QSAR were performed in silico. In vitro activity was evaluated by bioactivity staining based on the Ellman’s method. Results – In the Lipinski filter and ADME/Tox screening, all test compounds produced positive results, but in the target fishing, only one flavonoid could successfully target AchE. Molecular docking was performed on this flavonoid, and this compound gained the score as -13.5762. From the QSAR analysis the IC50 was found to be 1659.59 nM. Again, 100 derivatives were generated from the parent compound and docking was performed. The derivative number 20 was the best scorer i.e., -31.6392 and IC50 was predicted as 6.025 nM. Conclusion – Results indicated that flavonoids could be efficient inhibitors of AchE and thus, could be useful in the management of Alzheimer’s disease

Risky business: Disfluency as a design strategy

PosterThis paper presents a study examining the effects of disflu- ent design on audience perception of digital musical instru- ment (DMI) performance. Disfluency, defined as a barrier to effortless cognitive processing, has been shown to gen- erate better results in some contexts as it engages higher levels of cognition. We were motivated to determine if dis- fluent design in a DMI would result in a risk state that audiences would be able to perceive, and if this would have any effect on their evaluation of the performance. A DMI was produced that incorporated a disfluent characteristic: It would turn itself off if not constantly moved. Six phys- ically identical instruments were produced, each in one of three versions: Control (no disfluent characteristics), mild disfluency (turned itself off slowly), and heightened disflu- ency (turned itself off more quickly). 6 percussionists each performed on one instrument for a live audience (N=31), and data was collected in the form of real-time feedback (via a mobile phone app), and post-hoc surveys. Though there was little difference in ratings of enjoyment between the versions of the instrument, the real-time and qualita- tive data suggest that disfluent behaviour in a DMI may be a way for audiences to perceive and appreciate performer skill.This workis funded by the EPSRC through the Media and Arts Technology Centre for Doctoral Training (EP/G03723X/1), and Design for Virtuosity (EP/N005112/1

Investigation of sequential outbreaks of Burkholderia cepacia and multidrug-resistant extended spectrum β-lactamase producing Klebsiella species in a West African tertiary hospital neonatal unit: a retrospective genomic analysis

Background Sick newborns admitted to neonatal units in low-resource settings are at an increased risk of developing hospital-acquired infections due to poor clinical care practices. Clusters of infection, due to the same species, with a consistent antibiotic resistance profile, and in the same ward over a short period of time might be indicative of an outbreak. We used whole-genome sequencing (WGS) to define the transmission pathways and characterise two distinct outbreaks of neonatal bacteraemia in a west African neonatal unit. Methods We studied two outbreaks of Burkholderia cepacia and multidrug-resistant extended spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae in a neonatal unit that provides non-intensive care on the neonatal ward in the Edward Francis Small Teaching Hospital, Banjul, The Gambia. We used WGS to validate and expand findings from the outbreak investigation. We retrospectively sequenced all clinical isolates associated with each outbreak, including isolates obtained from swabs of ward surfaces, environmental fluid cultures, intravenous fluids, and antibiotics administered to newborns. We also sequenced historical B cepacia isolates associated with neonatal sepsis in the same ward. Results Between March 1 and Dec 31, 2016, 321 blood cultures were done, of which 178 (55%) were positive with a clinically significant isolate. 49 episodes of neonatal B cepacia bacteraemia and 45 episodes of bacteraemia due to ESBL-producing K pneumoniae were reported. WGS revealed the suspected K pneumoniae outbreak to be contemporaneous outbreaks of K pneumoniae (ST39) and previously unreported Klebsiella quasipneumoniae subspecies similipneumoniae (ST1535). Genomic analysis showed near-identical strain clusters for each of the three outbreak pathogens, consistent with transmission within the neonatal ward from extrinsically contaminated in-use intravenous fluids and antibiotics. Time-dated phylogeny, including retrospective analysis of archived bacterial strains, suggest B cepacia has been endemic in the neonatal ward over several years, with the Klebsiella species a more recent introduction. Interpretation Our study highlights the emerging threat of previously unreported strains of multidrug-resistant Klebsiella species in this neonatal unit. Genome-based surveillance studies can improve identification of circulating pathogen strains, characterisation of antimicrobial resistance, and help understand probable infection acquisition routes during outbreaks in newborn units in low-resource settings. Our data provide evidence for the need to regularly monitor endemic transmission of bacteria within the hospital setting, identify the introduction of resistant strains from the community, and improve clinical practices to reduce or prevent the spread of infection and resistance

Kinome rewiring reveals AURKA limits PI3K-pathway inhibitor efficacy in breast cancer.

Dysregulation of the PI3K-AKT-mTOR signaling network is a prominent feature of breast cancers. However, clinical responses to drugs targeting this pathway have been modest, possibly because of dynamic changes in cellular signaling that drive resistance and limit drug efficacy. Using a quantitative chemoproteomics approach, we mapped kinome dynamics in response to inhibitors of this pathway and identified signaling changes that correlate with drug sensitivity. Maintenance of AURKA after drug treatment was associated with resistance in breast cancer models. Incomplete inhibition of AURKA was a common source of therapy failure, and combinations of PI3K, AKT or mTOR inhibitors with the AURKA inhibitor MLN8237 were highly synergistic and durably suppressed mTOR signaling, resulting in apoptosis and tumor regression in vivo. This signaling map identifies survival factors whose presence limits the efficacy of targeted therapies and reveals new drug combinations that may unlock the full potential of PI3K-AKT-mTOR pathway inhibitors in breast cancer

Is the maternal health voucher scheme associated with increasing routine immunization coverage? Experience from Bangladesh

Bangladesh initiated the Maternal Health Voucher Scheme (MHVS) in 2007 to improve maternal and child health practices and bring equity to the mainstream of health systems by reducing financial and institutional barriers. In this study, we investigated whether the MHVS has an association with immunization coverage in a rural area of Bangladesh. Between 30 October 2016 and 15 June 2017, we carried out a cross-sectional survey in two low performing areas in terms of immunization coverage- Chattogram (erstwhile Chittagong division) and Sylhet division of Bangladesh. We calculated the coverage of fully immunized children (FIC) for 1151 children aged 12–23 months of age. We compared the coverage of FIC between children whose mothers enrolled in MHVS and children whose mother did not. We analyzed immunization coverage using crude odds ratio (OR) and adjusted OR (aOR) from binary logistic regression models. The overall coverage of FIC was 86%. Ninety-three percent children whose mothers were MHVS members were fully immunized whereas the percentage was 84% for the children of mothers who were not enrolled in MHVS. Multivariate analysis also shows that FIC coverage was higher for children whose mothers enrolled in MHVS compared to those children whose mothers did not; the aOR was 2.03 (95% confidence interval 1.11–3.71). MHVS provides a window for non-targeted benefits of childhood vaccination. Providing health education to pregnant mothers during prenatal care may motivate them to immunize their children. Programmes targeted for mothers during pregnancy, childbirth and post-natal may further increase utilization of priority health services such as childhood immunization

Analysis of the functional repertoire of a mutant form of survivin, K129E, which has been linked to lung cancer

Background Survivin is a protein that is normally present only in G2 and M-phases in somatic cells, however, in cancer cells, it is expressed throughout the cell cycle. A prosurvival factor, survivin is both an inhibitor of apoptosis and an essential mitotic protein, thus it has attracted much attention as a target for new oncotherapies. Despite its prevalence in cancer, reports of survivin mutations have mostly been restricted to loci within its promoter, which increase the abundance of the protein. To date the only published mutation within the coding sequence is an adenine > guanine substitution in exon 4. This polymorphism, which was found in a cohort of Korean lung cancer patients, causes a lysine > glutamic acid mutation (K129E) in the protein. However, whether it plays a causative role in cancer has not been addressed. Methods Using site directed mutagenesis we recapitulate K129E expression in cultured human cells and assess its anti-apoptotic and mitotic activities. Results K129E retains its anti-apoptotic activity, but causes errors in mitosis and cytokinesis, which may be linked to its reduced affinity for borealin. Conclusion K129E expression can induce genomic instability by introducing mitotic aberrations, thus it may play a causative role in cancer