Solar differential rotation and meridional flow: The role of a subadiabatic tachocline for the Taylor-Proudman balance

We present a simple model for the solar differential rotation and meridional circulation based on a mean field parameterization of the Reynolds stresses that drive the differential rotation. We include the subadiabatic part of the tachocline and show that this, in conjunction with turbulent heat conductivity within the convection zone and overshoot region, provides the key physics to break the Taylor-Proudman constraint, which dictates differential rotation with contour lines parallel to the axis of rotation in case of an isentropic stratification. We show that differential rotation with contour lines inclined by 10 - 30 degrees with respect to the axis of rotation is a robust result of the model, which does not depend on the details of the Reynolds stress and the assumed viscosity, as long as the Reynolds stress transports angular momentum toward the equator. The meridional flow is more sensitive with respect to the details of the assumed Reynolds stress, but a flow cell, equatorward at the base of the convection zone and poleward in the upper half of the convection zone, is the preferred flow pattern.Comment: 15 pages, 7 figure

Enhancement and evaluation of Skylab photography for potential land use inventories, part 1

The author has identified the following significant results. Three sites were evaluated for land use inventory: Finger Lakes - Tompkins County, Lower Hudson Valley - Newburgh, and Suffolk County - Long Island. Special photo enhancement processes were developed to standardize the density range and contrast among S190A negatives. Enhanced black and white enlargements were converted to color by contact printing onto diazo film. A color prediction model related the density values on each spectral band for each category of land use to the spectral properties of the various diazo dyes. The S190A multispectral system proved to be almost as effective as the S190B high resolution camera for inventorying land use. Aggregate error for Level 1 averaged about 12% while Level 2 aggregate error averaged about 25%. The S190A system proved to be much superior to LANDSAT in inventorying land use, primarily because of increased resolution

Magnetic flux generation and transport in cool stars

The Sun and other cool stars harbouring outer convection zones manifest magnetic activity in their atmospheres. The connection between this activity and the properties of a deep-seated dynamo generating the magnetic flux is not well understood. By employing physical models, we study the spatial and temporal characteristics of the observable surface field for various stellar parameters. We combine models for magnetic flux generation, buoyancy instability, and transport, which encompass the entire convection zone. The model components are: (1) a thin-layer alpha-Omega dynamo at the base of the convection zone; (2) buoyancy instabilities and the rise of flux tubes through the convection zone in 3D, which provides a physically consistent determination of emergence latitudes and tilt angles; and (3) horizontal flux transport at the surface. For solar-type stars and rotation periods longer than about 10 days, the latitudinal dynamo waves generated by the deep-seated alpha-Omega dynamo are faithfully reflected by the surface distribution of magnetic flux. For rotation periods of the order of two days, however, Coriolis acceleration of rising flux loops leads to surface flux emergence at much higher latitudes than the dynamo waves at the bottom of the convection zone reach. A similar result is found for a K0V star with a rotation period of two days. In the case of a rapidly rotating K1 subgiant, overlapping dynamo waves lead to noisy activity cycles and mixed-polarity fields at high latitudes.Comment: 14 pages, 14 figures. Accepted for publication in Astronomy & Astrophysic

Direct Interrogation of Viral Peptides Presented by the Class I HLA of HIV-Infected T Cells

Identification of CD8+ cytotoxic T lymphocyte (CTL) epitopes has traditionally relied upon testing of overlapping peptide libraries for their reactivity with T cells in vitro. Here, we pursued deep ligand sequencing (DLS) as an alternative method of directly identifying those ligands that are epitopes presented to CTLs by the class I human leukocyte antigens (HLA) of infected cells. Soluble class I HLA-A*11:01 (sHLA) was gathered from HIV-1 NL4-3-infected human CD4+ SUP-T1 cells. HLA-A*11:01 harvested from infected cells was immunoaffinity purified and acid boiled to release heavy and light chains from peptide ligands that were then recovered by size-exclusion filtration. The ligands were first fractionated by high-pH high-pressure liquid chromatography and then subjected to separation by nano-liquid chromatography (nano-LC)–mass spectrometry (MS) at low pH. Approximately 10 million ions were selected for sequencing by tandem mass spectrometry (MS/MS). HLA-A*11:01 ligand sequences were determined with PEAKS software and confirmed by comparison to spectra generated from synthetic peptides. DLS identified 42 viral ligands presented by HLA-A*11:01, and 37 of these were previously undetected. These data demonstrate that (i) HIV-1 Gag and Nef are extensively sampled, (ii) ligand length variants are prevalent, particularly within Gag and Nef hot spots where ligand sequences overlap, (iii) noncanonical ligands are T cell reactive, and (iv) HIV-1 ligands are derived from de novo synthesis rather than endocytic sampling. Next-generation immunotherapies must factor these nascent HIV-1 ligand length variants and the finding that CTL-reactive epitopes may be absent during infection of CD4+ T cells into strategies designed to enhance T cell immunity

Determination of Cellular Lipids Bound to Human CD1d Molecules

CD1 molecules are glycoproteins that present lipid antigens at the cell surface for immunological recognition by specialized populations of T lymphocytes. Prior experimental data suggest a wide variety of lipid species can bind to CD1 molecules, but little is known about the characteristics of cellular ligands that are selected for presentation. Here we have molecularly characterized lipids bound to the human CD1d isoform. Ligands were eluted from secreted CD1d molecules and separated by normal phase HPLC, then characterized by mass spectroscopy. A total of 177 lipid species were molecularly identified, comprising glycerophospholipids and sphingolipids. The glycerophospholipids included common diacylglycerol species, reduced forms known as plasmalogens, lyso-phospholipids (monoacyl species), and cardiolipins (tetraacyl species). The sphingolipids included sphingomyelins and glycosylated forms, such as the ganglioside GM3. These results demonstrate that human CD1d molecules bind a surprising diversity of lipid structures within the secretory pathway, including compounds that have been reported to play roles in cancer, autoimmune diseases, lipid signaling, and cell death

ISOPHOT Maps of NGC 6946 in the range λ 60-200μm.

We present maps of NGC 6946 at λ 61 and 205μm as well as scans through the galaxy at these and at 8 intermediate wavelengths. The bulk of the FIR luminosity arises from a diffuse disk component which extends beyond the 25mag/arcsec^2^ B isophote to at least the radius corresponding to the sensitivity limit. A FIR emission counterpart to the outlying HI disk is seen at both 61 and 205μm, for which a dust to HI mass ratio of 0.0046 is inferred from the 205μm brightness assuming a dust temperature of 15K. The disk scale length at both λ 61 and 205μm is similar to that seen in R-band

ISOPHOT Maps of NGC 6946 in the range λ 60-200μm.

We present maps of NGC 6946 at λ 61 and 205μm as well as scans through the galaxy at these and at 8 intermediate wavelengths. The bulk of the FIR luminosity arises from a diffuse disk component which extends beyond the 25mag/arcsec^2^ B isophote to at least the radius corresponding to the sensitivity limit. A FIR emission counterpart to the outlying HI disk is seen at both 61 and 205μm, for which a dust to HI mass ratio of 0.0046 is inferred from the 205μm brightness assuming a dust temperature of 15K. The disk scale length at both λ 61 and 205μm is similar to that seen in R-band