Results of construction of correlation fields with trend lines and determination of direction of relationships between studied indicators

The aim of work is construction of correlation fields with trend lines and determination of the directionality of relationships between the investigated indicators of endothelial dysfunction, hypoxia and oxidative stress. Our results indicate that Willebrand factor, endothelin-1, 2,3-diphosphoglycerate, MDA, diene conjugates and eNOS are informative markers in the pathogenesis of experimental diabetic retinopathy. As a result of the research, it was established that there is a direct linear relationship between the Willebrand factor indicator and malondialdehyde, since the points of the correlation field are practically located on a straight line. We trace a similar type of direct linear relationship between Willebrand factor and diene conjugates. The specified results confirm the mutually aggravating relationship between the development of endothelial dysfunction and oxidative stress. We trace an inverse linear relationship between Willebrand factor and peroxidase. With an increase in Willebrand factor indicators by stages, we trace a decrease in peroxidase indicators, which indicates a relationship between endothelial dysfunction and a weakening of antioxidant protection. As a result of the study, it was established that the first two indicators: malondialdehyde and diene conjugates have an almost direct linear relationship with endothelin-1 indicators. These results confirm the relationship between the functional and structural state of the endothelium and the development of oxidative stress. It was established that the peroxidase indicator has an inverse linear relationship with the endothelin-1 indicators, which, as in the case of the Willebrand factor, indicates a violation of antioxidant protection in the event of endothelial dysfunction. As a result of the analysis of the dependence of the indicators of 2,3 diphosphoglycerate, Willebrand factor and eNOS, it was found that in the first two pairs of indicators, there is an inverse linear relationship between the indicators. As eNOS levels increase by stage, levels of 2,3 diphosphoglycerate and Willebrand factor decrease. In the case of an inverse relationship between the parameters of the Willebrand factor and eNOS, a practically functional connection is observed, since the coefficient of determination is almost 1. The obtained results indicate that the violation of the structural and functional state of the endothelium and the development of hypoxia are interconnected with the violation of the physiological pathway of oxide synthesis nitrogen. The relationship between the indicators of 2,3 diphosphoglycerate and the Willebrand factor was revealed, which has a direct relationship between the variables, which once again confirms the aggravating interaction between hypoxia and endothelial dysfunction

Professionals’ perspectives on delivering conversation therapy in clinical practice

Background: Aphasia and other acquired language impairments have the potential to impact greatly on quality of life by disrupting everyday conversation. Different intervention approaches are available to speech and language therapists (SLTs), such as targeting the language impairment itself and/or addressing activity or participation barriers. Conversation therapy is one approach that is gaining in popularity, with a growing evidence base. However, it is not clear how SLTs currently use conversation approaches and what factors may influence delivery. Aims: To investigate how SLTs (i) define conversation therapy, (ii) deliver it in clinical practice, and identify (iii) any challenges faced. Methods & Procedures: An online survey and focus group explored how SLTs working in the south east of England currently deliver conversation therapy to support people with a range of communication disorders, in particular aphasia. Data were analysed using descriptive statistics and thematic content analysis. Outcomes & Results: A total of 50 SLTs completed the survey and 6 participants attended the focus group. Conversation therapy was found to be widely employed by participants, however there was considerable variation in the approaches used, and a number of major challenges were raised. SLTs reported delivering conversation therapy with a range of client groups and preferably working with the client and partner together. Conversation goals predominantly reflected an approach based on: (i) strategy use and/or total communication (TC), and (ii) Conversation Analysis. Three overarching themes around conversation therapy emerged from the focus group: (1) What is conversation therapy? (2) showing it works, and (3) complexities of delivering it. SLTs acknowledged the benefit of conversation therapy but felt they lacked the tools and skills needed to deliver it. Conclusions: SLTs wanted to use conversation therapy and desired clear outcome measures to demonstrate its effectiveness, but were not accessing the available evidence base, highlighting the ongoing difficulty of translating research into clinical practice. Whilst these data are limited by the small number of participants, the study provides a first view of how conversation therapy is articulated in practice. Further investigation of conversation therapy delivery is warranted with a larger sample of SLTs based across the United Kingdom, as is comparison with practice in other countries

Research of changes in the level of endothelin-1 in experimental diabetic retinopathy. comparison of methods of correction

Diabetic retinopathy (DR) according to the WHO is the main cause of decreased vision and blindness due to diabetes. The aim of the study: to compare the effectiveness of correction of vasoconstriction in experimental diabetic retinopathy under the influence of different methods of correction. Our results indicate a violation of the functional state of blood vessels on the 30th day of experimental diabetic retinopathy with subsequent progression of pathological changes on the 60th and 180th day of the study, as evidenced by a significant increase in endothelin-1 in the 2nd group (p <0.001), most pronounced in the 3rd stage.In the analysis of data of group №3 it was found that the correction of pathological conditions with hypoglycemic agents has a positive effect, but does not allow to significantly correct pathologically increased levels of endothelin-1, which indicates the need for additional correction tools other than hyperglycemia.The results of group 4 indicate that the involvement of a donor of nitric oxide and aflibercept in the correction of diabetic retinopathy corrects the level of the studied marker, the most pronounced effect is observed on the 180th day of the experiment, but normative values can not be achieved. It was found that in the 5th group, in which the correction of the simulated pathological condition was performed by reducing hyperglycemia, the introduction of aflibercept and bromfenac gives positive results, but less pronounced than in the 4th group, where L-arginine solution was added to the complex correction. .It was found that rats of group 6, which simulated diabetic retinopathy with subsequent correction of hyperglycemia, administration of aflibercept, L-carnitine and bromfenac have a pronounced effectiveness of the proposed method of correction compared to previous methods, the level of vasoconstriction stage and continues to recover in the following stages of the experiment, but does not reach the normative values. The obtained data suggest that the method of correction selected in group 7, which includes reduction of hyperglycemia, administration of aflibercept, solution of L-arginine and citicoline is most effective for normalizing vascular tone and structural and functional state of the endothelium

Dynamic of changes in the level of Interleukin 1B against the background of the development of experimental diabetic retinopathy and under the influence of various methods of correction

The aim of our research is investigating the changes in the level of interleukin 1β in experimental animals, which were simulated diabetic retinopathy and against the background of its correction. The obtained indicate that already on the 30th day of the development of experimental diabetic retinopathy, a significant increase in the pro-inflammatory cytokine level draws attention. Analyzing the data of group No. 3, it was established that the correction of the pathological condition with hypoglycemic agents has a positive effect but requires the involvement of additional means of correction in addition to the normalization of the level of glycemia. The results of the 4th experimental group indicate that the involvement of a nitric oxide donor and aflibercept in the correction of diabetic retinopathy has a positive effect on the reduction of the level of Interleukin 1β, but it does not reach an approximation to the norm. It is observed that the correction of the simulated pathological condition by reducing hyperglycemia, administration of aflibercept and bromfenac (group No. 5) gives positive results, but less pronounced than involvement in the complex correction of L-arginine solution in the study of nitric oxide synthases, in the analysis of the pro-inflammatory cytokine level the results are better. It is also worth noting that this method of correction is not characterized by longevity and on the 180th day there is already a pronounced decrease in its effectiveness. It was found that in rats in which diabetic retinopathy was modeled with subsequent correction of hyperglycemia, administration of aflibercept, L-carnitine and bromfenac (group No. 6), there is a pronounced effectiveness of the proposed method of correction in comparison with the previously considered methods. As for the correction applied in the 7th group, in the first and second stages, the inflammation indicator is more elevated compared to the two previous groups (No. 5 and No. 6), but in the third stage, a marked decrease in the level of the marker was established, which indicates the effectiveness of the correction of the 7th group at more distant stages of the experiment

Results of histological examination of experimental diabetic retinopathy and its correction with metformin, aflibercept, L-arginine solution and cyticolin

The purpose of the study was to analyze the histological changes in the structures of the eyeball in experimental diabetic retinopathy and its correction with metformin, aflibercept, L-arginine solution and citicoline. The obtained results indicate about the development of pathological changes in the retina against the background of the progression of experimental diabetes when streptozotocin is administered at dose 55 mg/kg of animal weight against the background of a high-fat diet. The development of simulated streptozotocin diabetes is accompanied by disturbance in the retina in the form of edematous shifts of fibers and swelling of the cells forming it, uneven distribution of cells of the ganglion layer and vacuolization of the cytoplasm some of them. The using of corrective drugs complex for metabolic disorders and angiopathy, which are characteristic of diabetes mellitus, affects on the retina condition. Under the influence of aflibercept, solution of L-arginine, citicoline, and metformin administration was established that edematous changes in its structural elements are significantly reduced, the number of vessels on the border with the retina visually increases, and the staining of the cells of the ganglion layer becomes more freshly

Features of the system of antioxidant protection and lipid peroxidation in microangiopathies against type 2 diabetes mellitus

The article presents the results of an experimental study of the AOР and LPО system in animals simulated complications of the microcirculatory tract (diabetic retinopathy, diabetic nephropathy) on the background of type 2 diabetes. It was found that in the group of animals with type 2 diabetes increased the level of DC, TBCproducts and MDA; catalase and FG levels decreased. In animals with simulated DN, AOP disorders were more pronounced compared to animals with DR. This trend can be explained by the fact that in addition to hyperglycemia in these animals, the excessive formation of free radicals contributes to metabolic shifts in ischemic areas of tissues and organs. Disturbance of balance in AOP and LPO in type 2 diabetes triggers a pathogenetic cascade of development of complications of the microcirculatory tract and is accompanied by a tendency to further increase the generation of reactive oxygen species and activation of POL in microangiopathies

Justification of the renoprotective action of the mixture of sodium chloride and sodium bicarbonate solutions in phenylhydrazine intoxication

The authors conducted a study on 35 white Wistar outbred rats to investigate the possibility of correcting acute kidney damage induced by the administration of phenylhydrazine at a dose of 100 mg/kg by introducing a mixture of sodium chloride and sodium bicarbonate solutions into the body. The research results identified changes in the kidneys upon phenylhydrazine administration, including the loss of some capillary glomeruli, eosinophilic deposits in Bowman's spaces and tubular lumens, and lymphoid infiltration in the interstitium. Rats receiving a mixture of sodium chloride and sodium bicarbonate solutions in their drinking water showed positive changes in their kidneys: no loss of capillary glomeruli was observed, and eosinophilic deposits were absent in most tubules. Lymphocyte aggregation was only observed around some renal vessels. The authors suggest that the intake of additional sodium and bicarbonate into the body, along with alkalinization of the primary urine, promotes the excretion of hemolysis products caused by phenylhydrazine, which contributes to renoprotection and preservation of renal parenchyma

Функціональний стан нирок після введення клітин фетальної печінки щурам з водним і сольовим режимом пиття за умов розвитку гострої сулемової нефропатії

The experiments on non linear white rats with acute sublimate nephropathy showed that in 7 days after intraperitoneal injection of fetal liver cells (FLC ) of rats plasma creatinine concentration decreased and proximal reabsorption of sodium ions increased independently of water or salt drinking regime. The increase of natremia in rats that used tap water excluded regulatory influence of FLC on the processes of maintenance of sodium balance under the conditions of experiment. Usage of 0.9 % NaCl solution leads to increase of glomerular filtration rate, activation of tubular transport of sodium ions with predominant elevation of reabsorption in the proximal part of nephron, reduction of sodium ions loss with urination. That points to substantial role of salt drinking regimen in additional beneficial effects of FLC on renal processes in rats with acute sublimate nephropathy.В экспериментах на нелинейных белых крысах с острой сулемовой нефропатией установлено, что через 7 дней после внутрибрюшного введения клеток фетальной печени (КФП) крысам уменьшалась концентрация креатинина в плазме крови и увеличивалась проксимальная реабсорбция ионов натрия независимо от водного или солевого режима питья. Повышение уровня натриемии у животных, употреблявших водопроводную воду, исключало регуляторное влияние КФП на процессы обеспечения натриевого баланса в этих условиях эксперимента. При употреблении 0,9 % раствора NaCl увеличивалась скорость клубочковой фильтрации, активировался канальцевый транспорт ионов натрия с преимущественным повышением реабсорбции в проксимальном отделе нефрона, уменьшалась потеря ионов натрия с мочой, что указывает на существенную роль солевого режима питья для дополнительного благоприятноговоздействия КФП на почечные процессы у крыс с острой сулемовой нефропатией.В експериментах на нелінійних білих щурах із гострою сулемовою нефропатією встановлено, що через 7 днів після внутрішньочеревного введення клітин фетальної печінки (КФП) щурам зменшувалась концентрація креатиніну в плазмі крові та збільшувалась проксимальна реабсорбція іонів натрію незалежно від водного чи сольового режиму пиття. Підвищення рівня натріємії у тварин, які вживали водопровіднуводу, виключало регуляторний вплив КФП на процеси забезпечення натрієвого балансу в організмі за цихумов експерименту. При вживанні 0,9 % розчину NaCl збільшувалась швидкість клубочкової фільтрації,активувався канальцевий транспорт іонів натрію з переважним підвищенням реабсорбції в проксимальному відділі нефрону, зменшувалась втрата іонів натрію із сечею, що вказує на значну роль сольового режиму пиття для додаткового сприятливого впливу КФП на ниркові процеси у щурів із гострою сулемовою нефропатією

Гостре ураження нирок при запальних процесах і шляхи їх корекції = Acute kidney damage in inflammatory processes and ways of their correction

Sirman V. M., Boris R. M., Nykytenko O. P., Zukow W., Gozhenko A. I. Гостре ураження нирок при запальних процесах і шляхи їх корекції = Acute kidney damage in inflammatory processes and ways of their correction. Journal of Education, Health and Sport. 2016;6(1):349-368. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.47347http://ojs.ukw.edu.pl/index.php/johs/article/view/3417https://pbn.nauka.gov.pl/works/719461  The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).755 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 01.01.2016. Revised 12.01.2016. Accepted: 31.01.2016.   УДК 616.61-002 Гостре ураження нирок при запальних процесах і шляхи їх корекції В. М. Сірман, Р. М. Борис, О. П. Никитенко, В. A. Жуков, А. І. Гоженко Державне підприємство «Український науково-дослідний інститут медицини транспорту МОЗ України», м. Одеса, УкраинаUniwersytet Kazimierza Wielkiego, Bydgoszcz, Polska РезюмеМетою дослідження було вивчення функціонального стану та механізмів порушеннями нирок при експериментальному перитоніті, а також при ад’ювантному артриті Пірсона у щурів. Для вирішення поставлених задач проведені серії експериментів in vivo. У роботі використано 189 самців білих щурів з середньою масою тіла 0,193±0,018 кг. Дослідження проводили через 2, 4, 6 і 12 міс. після індукції ад’ювантного артриту Пірсона.  При експериментальному перитоніті, проведено 7 серій експериментів на 486 самцях білих щурів з масою тіла 0,17-0,30 кг. Встановлено, що при обох експериментальних моделях запалення виникають однотипні порушення функції нирок, які виявляються у зменшенні діурезу, зростанні екскреції білка та натрію, що пов’язано як із падінням швидкості клубочкової фільтрації, так і канальцевої реабсорбції води та натрію. Їх розвиток відрізняється у часі та залежить від строків виникнення та динаміки запального процесу. Ключові слова: гостре ураження нирок, ХХН, ХНН, ШКФ. Acute kidney damage in inflammatory processes and ways of their correction V. M. Sirman, R. M. Boris, O. P. Nykytenko, W. Zukow, A. I. Gozhenko SE "Ukrainian Scientific Research Institute of Transport Medicine, Ministry of Health of UkraineKazimierz Wielki University, Bydgoszcz, Poland AbstractThe aim of the study was to investigate functional status and mechanisms of disorders of the kidneys in experimental peritonitis, as well as adjuvant arthritis in rats Pearson. To solve this problem, a series of experiments in vivo. The paper used 189 male albino rats with an average body weight of 0,193 ± 0,018 kg. The study was conducted at 2, 4, 6 and 12 months. After induction of adjuvant arthritis Pearson. In experimental peritonitis, 7 held a series of experiments on 486 white male rats weighing 0,17-0,30 kg. Found that in both experimental models of inflammation arising same type of renal dysfunction, which are to reduce urine output, increasing excretion of sodium and protein, which is associated with a drop in glomerular filtration rate and tubular reabsorption of water and sodium. Their development is different in time and depends on the timing of the emergence and dynamics of inflammation. Keywords: acute kidney damage, CKD, chronic renal failure, GFR

Вплив трансплантації ембріональних прогеніторних клітин на функцію нирок = Effect of transplantation embryonic progenitor cells on renal function

Sirman V. M., Borys R. M., Nykytenko O. P., Zukow W., Gozhenko A. I. Вплив трансплантації ембріональних прогеніторних клітин на функцію нирок = Effect of transplantation embryonic progenitor cells on renal function. Journal of Education, Health and Sport. 2016;6(2):349-366. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.56017http://ojs.ukw.edu.pl/index.php/johs/article/view/3614 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).755 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2016;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 05.01.2016. Revised 12.02.2016. Accepted: 27.02.2016. УДК: 616.61-008-089.843:615.361.013  В. М. Сірман, Р. М. Борис, О. П. Никитенко, В. Жуков, А. І. Гоженко Український науково-дослідний інститутмедицини транспорту МОЗ України, м. Одеса, УкраинаUMK w ToruniuРезюмеМетою нашого дослідження було вивчення  впливу трансплантації ембріональних прогеніторних клітин на функцію нирок при експериментальному перитоніті та при ад’ювантному артриті Пірсона у щурів. У роботі використано 189 самців білих щурів з середньою масою тіла 0,193±0,018 кг. Дослідження проводили через 2, 4, 6 і 12 міс. після індукції ад’ювантного артриту Пірсона.  При експериментальному перитоніті, проведено 7 серій експериментів на 486 самцях білих щурів з масою тіла 0,17-0,30 кг. Встановлено, що при обох експериментальних моделях запалення трансплантація ембріональних прогеніторних клітин сприяє значному підвищенню діурезу за рахунок збільшення швидкості клубочкової фільтрації, що суттєво знижує вміст креатиніну у плазмі крові. Крім того, під впливом трансплантації ембріональних прогеніторних клітин спостерігається дворазове зниження втрат білка з кінцевою сечею, стандартизованих за об’ємом клубочкового фільтрату. Ключові слова: гостре ураження нирок, ШКФ, трансплантація ембріональних прогеніторних клітин.   EFFECT OF TRANSPLANTATION EMBRYONIC PROGENITOR CELLS ON RENAL FUNCTION V. M. Sirman, R. M. Borys, O. P. Nykytenko, W. Zukow, A. I. Gozhenko Ukrainian Scientific Research Institute of Transport Medicine, Ministry of Health of UkraineUMK w ToruniuResumeThe aim of our study was to examine the influence of transplantation of embryonic progenitor cells in renal function in experimental peritonitis and in adjuvant arthritis in rats Pearson. The paper used 189 male albino rats with an average body weight of 0,193 ± 0,018 kg. The study was conducted at 2, 4, 6 and 12 months. After induction of adjuvant arthritis Pearson. In experimental peritonitis, held 7 series of experiments on 486 white male rats weighing 0,17-0,30 kg. Found that in both experimental models of inflammation transplantation of embryonic progenitor cells contributes to a significant increase in urine output by increasing glomerular filtration rate, which significantly reduces creatinine in blood plasma. In addition, under the influence of transplantation of embryonic progenitor cells there is a twofold decrease loss of protein from the final urine volume standardized glomerular filtrate. Keywords: acute kidney damage, GFR, Transplantation of embryonic progenitor cells