PREPARACIÓN DE UN COMPLEJO ANTIMONIO- -CICLODEXTRINA PARA EL TRATAMIENTO DE LEISHMANIASIS

Existe la necesidad de mejorar las limitaciones en el uso clínico de los antimoniales pentavalentes para el tratamiento de la leishmaniasis. En este trabajo se obtuvo un nuevo antimonial formado a partir de antimonio y la -ciclodextrina. El complejo presenta potencial leishmanicida y fue caracterizado a través de anál isis térmica (TG/DTG, DSC), conductividad, análisis elemental, absorción atómica, espectrometría vibracional en la región de infrarrojo, difracción de rayos-X y RMN de 1H y Yde 13C. Los resultados indican la formación del complejo en una relación equimolar y sugieren que el oxigeno del C-2 sea el sitio de enlace del antimonio a la -ciclodextrina

Electrospun nanofibers of polyCD/PMAA polymers and their potential application as drug delivery system

Herein, we used an electrospinning process to develop highly efficacious and hydrophobic coaxial nanofibers based on poly-cyclodextrin (polyCD) associated with poly(methacrylic acid) (PMAA) that combines polymeric and supramolecular features for modulating the release of the hydrophilic drug, propranolol hydrochloride (PROP). For this purpose, polyCD was synthesized and characterized, and its biocompatibility was assessed using fibroblast cytotoxicity tests. Moreover, the interactions between the guest PROP molecule and both polyCD and $\beta$CD were found to be spontaneous. Subsequently, PROP was encapsulated in uniaxial and coaxial polyCD/ PMAA nanofibers. A lower PROP burst effect (reduction of approximately 50%) and higher modulation were observed from the coaxial than from the uniaxial fibers. Thus, the coaxial nanofibers could potentially be a useful strategy for developing a controlled release system for hydrophilic molecules.Comment: 10 pages, 8 figures + Supplementary Data (4 pages, 6 figures

Efeito antifúngico de filmes poliméricos que contêm óleo essencial de Schinus terebinthifolius nos biofilmes de Candida albicans

Introduction: Candida albicans is a commensal microorganism in humans, which can cause oral candidiasis in people with immune deficiencies or ill-fitting dentures. C. albicans is becoming more resistant to the drugs used in its treatment, causing the need for the development of new drugs, which would not damage the host´s cells. There are many studies on the antimicrobial activity of the leaves, stem and roots of Schinus terebinthifolius, with few studies about its fruits. The aim of this study was to investigate the antifungal effect of polymeric films of hydroxypropyl methylcellulose (HPMC) containing the essential oil of the fruits of S. terebinthifolius against biofilms of C. albicans. Materials and methods: The oil was prepared from the fruits of S. terebinthifolius and incorporated into polymeric films of HPMC using concentrations of 0.146, 0.073 e 0.037 g.mL-1. The minimum inhibitory concentration (MIC) was determined by micro dilution, wherein the minimum inhibitory concentration was 0.78 %. The antifungal effect of the films was tested using agar in petri dishes and acrylic resin blocks, both contaminated with C. albicans. Results: The polymeric films containing the essential oil had average inhibition zones of 24 mm, 21 mm and 20 mm for the concentrations 0.037, 0.073 and 0.146 g.mL-1, respectively. Conclusion: The treated resin blocks showed no fungal growth afterwards, with the exception of the control blocks. The use of antimicrobial films containing natural products like S. terebinthifolius in the treatment of diseases such as oral candidiasis associated to prosthetic seems promising decreasing the possibility of side effects.Introducción: Candida albicans es un microorganismo comensal en humanos que puede causar candidiasis oral en personas con deficiencias inmunitarias o dentaduras mal ajustadas. C. albicans se está volviendo más resistente a los medicamentos utilizados para su tratamiento. Esto hace que sea necesario desarrollar nuevos medicamentos que no dañen las células huésped. Existen muchos estudios sobre la actividad antimicrobiana de hojas, tallo y raíces de Schinus terebinthifolius, pero pocos estudios se centran en sus frutos. El objetivo de este estudio es investigar el efecto antifúngico de las películas poliméricas de hidroxipropilmetilcelulosa (hpmc) que contiene el aceite esencial de los frutos de S. terebinthifolius en biopelículas de C. albicans. Materiales y métodos: el aceite se preparó a partir de los frutos de S. terebinthifolius y se incorporó en películas poliméricas de hpmc con concentraciones de 0,146, 0,073 y 0,037 g.mL-1. La concentración mínima inhibitoria (mic) se determinó mediante microdilución, cuya concentración inhibitoria mínima fue de 0,78%. El efecto antifúngico de las películas se probó con agar en placas de Petri y bloques de resina acrílica, ambos de ellos contaminados con C. albicans. Resultados: las películas poliméricas que contenían aceite esencial presentaron zonas de inhibición promedio de 24 mm, 21 mm y 20 mm para las concentraciones 0,037, 0,073 y 0,146 g.mL-1, respectivamente. Conclusión: los bloques de resina tratados no mostraron crecimiento fúngico posterior, con la excepción de los bloques de control. El uso de películas antimicrobianas que contienen productos naturales como S. terebinthifolius en el tratamiento de enfermedades como la candidiasis oral asociada a la prótesis parece prometedor y con menor posibilidad de que se desarrollen efectos secundarios.Introdução: Candida albicans é um microrganismo comensal em humanos que pode causar candidíase oral em pessoas com deficiências imunológicas ou próteses mal ajustadas. C. albicans está se tornando mais resistente aos medicamentos usados, pelo qual é necessário desenvolver uns novos que não prejudiquem as células hospedeiras. Existem muitos estudos sobre a atividade antimicrobiana de folhas, caules e raízes de Schinus terebinthifolius, mas poucos estudos consideram seus frutos.   Objetivo: investigar o efeito antifúngico dos filmes poliméricos de hidroxipropilmetilcelulose (hpmc) contendo o óleo essencial dos frutos de S. terebinthifolius em biofilmes de C. albicans.   Materiais e métodos: preparou-se o óleo a partir dos frutos de S. terebinthifolius e incorporou-se em filmes poliméricos de hpmc com concentrações de 0,146, 0,073 e 0,037 g.mL-1. A concentração inibitória mínima (cim) foi determinada através de microdiluição, cuja concentração inibitória mínima foi de 0,78%. O efeito antifúngico dos filmes foi testado com ágar em placas de Petri e blocos de resina acrílica, ambos contaminados com C. albicans.   Resultados: os filmes poliméricos contendo óleo essencial apresentaram zonas de inibição médias de 24 mm, 21 mm e 20 mm para as concentrações 0,037, 0,073 e 0,146 g.mL-1, respectivamente.   Conclusão: os blocos de resina não apresentaram crescimento fúngico posterior, com exceção dos blocos de controle. O uso de filmes antimicrobianos contendo produtos naturais, tais como S. terebinthifolius, parece promissor para o tratamento de doenças como a candidíase oral associada à prótese. Estes filmes reduzem a possibilidade de desenvolvimento de efeitos colaterais

Preparation of a ferrofluid using cyclodextrin and magnetite

A ferrofluid has been obtained from magnetite and beta-cyclodextrin, with the formation of an inclusion complex. The magnetite and beta-cyclodextrin complex was characterized by FTIR spectroscopy, X-ray diffraction, thermal analysis (TG/DTA), transmission electron microscopy (TEM) and atomic absorption spectroscopy. As far as we know, this is the first report on an inclusion compound between a metal oxide and cyclodextrins

Preparação do composto de associação entre citrato de ródio(II) e β-ciclodextrina

Inclusion compound of rhodium(II) citrate with β-cyclodextrin in a 1:1 molar ratio was prepared using freeze-drying method. X-ray diffactometry, thermal analysis (TG/DTG/DSC), infrared and ¹H-NMR with ¹H spin lattice relaxation (¹H T1) measurements and 13C techniques were used to characterize the system prepared. The results indicated the formation of inclusion or association compounds between rhodium(II) citrate and β-cyclodextrin

Improvement of genistein content in solid genistein/-cyclodextrin complexes β

Genistein:β-cyclodextrin complexes with high drug loading (19.22%) were prepared by freeze-drying and characterized by differential scanning calorimetry and hydrogen nuclear magnetic resonance spectroscopy. The spatial configuration of the complex was proposed by means of 2D-NOESY experiment combined with molecular modeling. According to the results obtained, the interaction of genistein with β -cyclodextrin in a 1:1 complex is supposed to occur mainly through the insertion of the guest A-ring in cyclodextrin cavity, without rule out the possibility of inclusion through the B-ring, as previously reported in the literature