STANDARD MANUFACTURING PROCESS OF DVIGUNA BALIJARITA RASASINDURA

Introduction: Preparation of herbo-mineral compounds in Ayurveda revolves around mercury, considered as the prime among all minerals. Sulphur is again an important element used in many disorders and applied substance in different Ayurvedic formulations, considered as the antidote for the harmful mercurials. Kajjali is prepared by grinding mercury and sulphur. The prepared Kajjali used as a raw material for Rasasindura preparation with Kupipakva method. Kupipakva method is developed by ancient Rasaacharya for the preparation of heat treated mercurial. Dviguna gandhak jarita parada is considered more potent compare to Samaguna bali jarita. Objective: To study Kupipakva kalpana with preparation of Dviguna-bali jarita Rasa sindura. Methodology: Whole process is divided and performed as Sodhana of contents (Hingula and gandhaka), Hingulotth parada extraction, Kajjali preparation, preparation of Dviguna balijarita Rasa sindura. Observation: Hingula after Shodhana became soft and lusterless compound and Gandhaka becomes granular and brittle. Hinguloth parada extraction by Kanduka yantra method shown more yield compared to other methods. Kajjali preparation took 96 hours of Mardana of contents with Nischandra property. It took 48 hours for Dviguna Bali Jarita Rasasindura preparation by Kupipakva kalpana method with a yield of 106g. Conclusion: Preparation of Dviguna Balijarita Rasasindura needs more duration of heat treatment in every step of graded heating pattern of Kupipakva kalpana. The heating pattern is proportional to its therapeutic efficacy

Separation of Azeotropic Mixture (Water-Ethanol) Using Ionic Liquid

Azeotrope mixture is a liquid mixture which has constant boiling point and at a given pressure and temperature the liquid composition and vapor composition is the same where it is the equilibrium point. It has two or more component mixed together to form ‘inseparable” compositions. In my project, I took ethanol and water to represent the azeotropic mixture which will be formed at 96% volume of ethanol and 4% volume of water. With the existences of azeotropic mixture it will complicated the operating region of vapor liquid envelope of the equilibrium temperature. There are four main objectives of this project which are: • To find a replacement for benzene or toluene as an entrainer. • To investigate the possibilities of using ionic liquid to separate azeotropic mixture (ethanol and water). • To evaluate the performances of using ionic liquid to separate azeotropic mixture (ethanol and water). • To compare the results of using different ionic liquid to separate azeotropic mixture (ethanol and water). The scope of study for this separation process of ethanol and water using ionic liquid is conducted by performing experimental analysis purity of the separation. This is done by collecting, analyzing and documenting data obtained from simple distillation. For my project I will be using the simple distillation method to separate water and ethanol mixture. With that, I will be using three different types of ionic liquids to compare the performances of separating the water and ethanol mixture. So at first, simple distillation will be performed only for water and ethanol mixture followed by addition of benzene and toluene and also the three different ionic liquids. The results are plotted and it will be discussed according to the works that have been done. Using three different types of ionic liquids will give the comparison which ionic liquid will be the best choice to separate water and ethanol mixture. Just to give an overview, 2-hydroxyethylammonium Lactate gives the highest value of ethanol been distillate out. Following are the graphs that are plotted: i). Purity vs. volume of ionic liquid. ii). Volume of ethanol distillate vs. volume of ionic liquid By adding three different types of ionic liquids, water-ethanol mixture can break the azeotrope but 2-hydroxyethylammonium Lactate gives the highest value of ethanol been distillate out. Therefore, it opens new possibilities as an entrainer for this ionic liquid

Study of ambient air pollutants over Rishikesh at foothills of north-western Indian Himalaya

49-60Air quality parameters measured over Rishikesh city of Uttarakhand, where a large number of visitors and pilgrimage stay and pass by throughout the year are analyzed and studied. Such intensive human activities lead to the impacts and its manifestation on regional scale environment. Due to consistent infrastructure development and the increased vehicular emission due to transport, a large amount of particulate matters is added to the ambient environment and affects the air quality adversely. Here an attempt is made to understand the growth in level of ambient air pollutants through available measurement. In this study particulate matter (PM10), suspended particulate matters (SPMs) and the concentration of gaseous pollutants (SO2 and NO2) from 2011 to 2014 are measured and analyzed. It is observed that concentrations of the pollutants increase during pre-monsoon season as compared to the winter and monsoon seasons. In addition, PM10 and SPM concentrations are found about two times higher than the prescribed national standard. SO2 and NO2 levels are found within the limits as proposed by the Central Pollution Control Board (CPCB) New Delhi, India. A detailed statistical analysis is carried out on the basis of monthly mean values of the observed pollutants. The PM10 exhibits a significant positive correlation with SO2 and NO2 concentrations. In addition, back trajectories show partly long-term transport from North Africa, Saudi Arabia and central Asian region in contributing over the region with local emission that is considered to be the main and direct cause of increasing trend of the pollutants

Amyloid Precursor-like Protein 2 Expression Increases during Pancreatic Cancer Development and Shortens the Survival of a Spontaneous Mouse Model of Pancreatic Cancer.

In the United States, pancreatic cancer is a major cause of cancer-related deaths. Although substantial efforts have been made to understand pancreatic cancer biology and improve therapeutic efficacy, patients still face a bleak chance of survival. A greater understanding of pancreatic cancer development and the identification of novel treatment targets are desperately needed. Our analysis of gene expression data from patient samples showed an increase in amyloid precursor-like protein 2 (APLP2) expression within primary tumor epithelium relative to pancreatic intraepithelial neoplasia (PanIN) epithelial cells. Augmented expression of APLP2 in primary tumors compared to adjacent stroma was also observed. Genetically engineered mouse models of spontaneous pancreatic ductal adenocarcinoma were used to investigate APLP2\u27s role in cancer development. We found that APLP2 expression intensifies significantly during pancreatic cancer initiation and progression in the LSL-KrasG12D/+; LSL-Trp53R172H/+; Pdx-1-Cre (KPC) mouse model, as shown by immunohistochemistry analysis. In studies utilizing pancreas-specific heterozygous and homozygous knockout of APLP2 in the KPC mouse model background, we observed significantly prolonged survival and reduced metastatic progression of pancreatic cancer. These results demonstrate the importance of APLP2 in pancreatic cancer initiation and metastasis and indicate that APLP2 should be considered a potential therapeutic target for this disease

Replication timing maintains the global epigenetic state in human cells

ACKNOWLEDGMENTS We thank R. Didier and B. Alexander of the FSU Flow Cytometry and Confocal Microscopy Facilities for their help with flow cytometry and fluorescence-activated cell sorting for this project. Thanks to A. Brown of the FSU Biological Science Core Labs and to Y. Yang and C. Vied of the FSU Translational Labs. Thanks to S. R. Westermann of SCIGRAPHIX for generating the model figure. Thanks to B. van Steensel, J. Phillips-Cremins, and P. Fraser for critical reading of the manuscript. Funding: This work was supported by NIH grant GM083337 to D.M.G., GM035463 to V.G.C., and GM085354 to D.M.G., S.D., and V.G.C. D.L. is supported by the Hong Kong Research Grant Council (ECS 26104216). T.B. is supported by the William C. and Joyce C. O’Neil Charitable Trust, Memorial Sloan Kettering Single Cell Sequencing InitiativePeer reviewedPostprin

Regulatory T Cells Suppress T Cell Activation at the Pathologic Site of Human Visceral Leishmaniasis

Suppression of T cell response is thought to be involved in the pathogenesis of visceral leishmaniasis (VL). Regulatory T cell (Treg) mediated immune-suppression is reported in animal models of Leishmania infection. However, their precise role among human patients still requires pathologic validation. The present study is aimed at understanding the frequency dynamics and function of Treg cells in the blood and bone marrow (BM) of VL patients. The study included 42 parasitologically confirmed patients, 17 healthy contact and 9 normal bone marrow specimens (NBM). We show i) the selective accumulation of Treg cells at one of the disease inflicted site(s), the BM, ii) their in vitro expansion in response to LD antigen and iii) persistence after successful chemotherapy. Results indicate that the Treg cells isolated from BM produces IL-10 and may inhibit T cell activation in IL-10 dependent manner. Moreover, we observed significantly higher levels of IL-10 among drug unresponsive patients, suggesting their critical role in suppression of immunity among VL patients. Our results suggest that IL-10 plays an important role in suppression of host immunity in human VL and possibly determines the efficacy of chemotherapy

Challenges in QCD matter physics - The Compressed Baryonic Matter experiment at FAIR

Substantial experimental and theoretical efforts worldwide are devoted to explore the phase diagram of strongly interacting matter. At LHC and top RHIC energies, QCD matter is studied at very high temperatures and nearly vanishing net-baryon densities. There is evidence that a Quark-Gluon-Plasma (QGP) was created at experiments at RHIC and LHC. The transition from the QGP back to the hadron gas is found to be a smooth cross over. For larger net-baryon densities and lower temperatures, it is expected that the QCD phase diagram exhibits a rich structure, such as a first-order phase transition between hadronic and partonic matter which terminates in a critical point, or exotic phases like quarkyonic matter. The discovery of these landmarks would be a breakthrough in our understanding of the strong interaction and is therefore in the focus of various high-energy heavy-ion research programs. The Compressed Baryonic Matter (CBM) experiment at FAIR will play a unique role in the exploration of the QCD phase diagram in the region of high net-baryon densities, because it is designed to run at unprecedented interaction rates. High-rate operation is the key prerequisite for high-precision measurements of multi-differential observables and of rare diagnostic probes which are sensitive to the dense phase of the nuclear fireball. The goal of the CBM experiment at SIS100 (sqrt(s_NN) = 2.7 - 4.9 GeV) is to discover fundamental properties of QCD matter: the phase structure at large baryon-chemical potentials (mu_B > 500 MeV), effects of chiral symmetry, and the equation-of-state at high density as it is expected to occur in the core of neutron stars. In this article, we review the motivation for and the physics programme of CBM, including activities before the start of data taking in 2022, in the context of the worldwide efforts to explore high-density QCD matter.Comment: 15 pages, 11 figures. Published in European Physical Journal

Age and the association between apolipoprotein E genotype and Alzheimer disease: A cerebrospinal fluid biomarker-based case-control study

Background: The ε4 allele of apolipoprotein E (APOE) gene and increasing age are two of the most important known risk factors for developing Alzheimer disease (AD). The diagnosis of AD based on clinical symptoms alone is known to have poor specificity; recently developed diagnostic criteria based on biomarkers that reflect underlying AD neuropathology allow better assessment of the strength of the associations of risk factors with AD. Accordingly, we examined the global and age-specific association between APOE genotype and AD by using the A/T/N classification, relying on the cerebrospinal fluid (CSF) levels of β-amyloid peptide (A, β-amyloid deposition), phosphorylated tau (T, pathologic tau), and total tau (N, neurodegeneration) to identify patients with AD. Methods and findings: This case–control study included 1,593 white AD cases (55.4% women; mean age 72.8 [range = 44–96] years) with abnormal values of CSF biomarkers from nine European memory clinics and the American Alzheimer’s Disease Neuroimaging Initiative (ADNI) study. A total of 11,723 dementia-free controls (47.1% women; mean age 65.6 [range = 44–94] years) were drawn from two longitudinal cohort studies (Whitehall II and Three-City), in which incident cases of dementia over the follow-up were excluded from the control population. Odds ratio (OR) and population attributable fraction (PAF) for AD associated with APOE genotypes were determined, overall and by 5-year age categories. In total, 63.4% of patients with AD and 22.6% of population controls carried at least one APOE ε4 allele. Compared with non-ε4 carriers, heterozygous ε4 carriers had a 4.6 (95% confidence interval 4.1–5.2; p < 0.001) and ε4/ε4 homozygotes a 25.4 (20.4–31.2; p < 0.001) higher OR of AD in unadjusted analysis. This association was modified by age (p for interaction < 0.001). The PAF associated with carrying at least one ε4 allele was greatest in the 65–70 age group (69.7%) and weaker before 55 years (14.2%) and after 85 years (22.6%). The protective effect of APOE ε2 allele for AD was unaffected by age. Main study limitations are that analyses were based on white individuals and AD cases were drawn from memory centers, which may not be representative of the general population of patients with AD. Conclusions: In this study, we found that AD diagnosis based on biomarkers was associated with APOE ε4 carrier status, with a higher OR than previously reported from studies based on only clinical AD criteria. This association differs according to age, with the strongest effect at 65–70 years. These findings highlight the need for early interventions for dementia prevention to mitigate the effect of APOE ε4 at the population level

Multidisciplinary consensus guideline for the diagnosis and management of spontaneous intracranial hypotension

BACKGROUND: We aimed to create a multidisciplinary consensus clinical guideline for best practice in the diagnosis, investigation and management of spontaneous intracranial hypotension (SIH) due to cerebrospinal fluid leak based on current evidence and consensus from a multidisciplinary specialist interest group (SIG). METHODS: A 29-member SIG was established, with members from neurology, neuroradiology, anaesthetics, neurosurgery and patient representatives. The scope and purpose of the guideline were agreed by the SIG by consensus. The SIG then developed guideline statements for a series of question topics using a modified Delphi process. This process was supported by a systematic literature review, surveys of patients and healthcare professionals and review by several international experts on SIH. RESULTS: SIH and its differential diagnoses should be considered in any patient presenting with orthostatic headache. First-line imaging should be MRI of the brain with contrast and the whole spine. First-line treatment is non-targeted epidural blood patch (EBP), which should be performed as early as possible. We provide criteria for performing myelography depending on the spine MRI result and response to EBP, and we outline principles of treatments. Recommendations for conservative management, symptomatic treatment of headache and management of complications of SIH are also provided. CONCLUSIONS: This multidisciplinary consensus clinical guideline has the potential to increase awareness of SIH among healthcare professionals, produce greater consistency in care, improve diagnostic accuracy, promote effective investigations and treatments and reduce disability attributable to SIH