Characteristics of a Green Turtle (Chelonia mydas) Assemblage in Northwestern Florida Determined During a Hypothermic Stunning Event

A hypothermic stunning event (i.e., cold-stunning event) during late Dec. 2000 and early Jan. 2001 involving an unprecedented number of sea turtles provided an opportunity to characterize the green turtle (Chelonia mydas) assemblage in St. Joseph Bay (Gulf County, Florida). In addition to 388 green turtles, the 401 cold-stunned turtles comprised 10 Kemp\u27s ridleys (Lepidochelys kempii) and three loggerheads (Caretta caretta). Most (337/401) of the turtles survived and were eventually released. To place this event in perspective, we categorize sea turtle cold-stunning events in the eastern United States as either acute or chronic. Acute cold-stunning events, like the one in St. Joseph Bay, occur only during unusually cold winters in shallow-water areas (\u3c 2m), where sea turtles are year-round residents. These are short-lived (\u3c 2 wk) events with low mortality rates (\u3c 30%) that affect principally green turtles. Chronic cold-stunning events occur every winter in areas where sea turtles are seasonal residents. These are long-lived (1-3 mo) events with high mortality rates (\u3e 60%) that affect primarily Kemp\u27s ridleys. All of the green turtles from St. Joseph Bay were neritic-phase juveniles, and the mean straight-line carapace length of this group was 36.6 cm (range = 25.0-75.3 cm, SD = 8.9). This assemblage of juvenile green turtles is the first documented along the northern Gulf of Mexico. Sequencing of mtDNA (mitochondrial DNA) from tissue samples taken from 255 of the green turtles revealed that about 81% were from the nesting populations in the United States (Florida) and Mexico (Yucatan). This assemblage is unusual in the United States because it does not have a substantial representation from the nesting population in Costa Rica (Tortuguero), the Atlantic\u27s largest green turtle nesting population. Based on necropsies of 51 of the green turtles, the sex ratio of this assemblage was female-biased (3.25 females: 1 male), which may be a result of warm incubation temperatures on the nesting beaches in Florida. The majority of the material found in the gastrointestinal tracts of the green turtles that died was turtle grass (Thalassia testudinum). This was the first time turtle grass has been identified as the primary diet of juvenile green turtles anywhere in the continental United States. Green turtles in St. Joseph Bay appear to have few direct threats, but the seagrass upon which these turtles primarily forage has suffered extensive damage from boat propellers

Investigation of Electron Transfer-Based Photonic and Electro-Optic Materials and Devices

Montanaâs state program began its sixth year in 2006. The projectâs research cluster focused on physical, chemical, and biological materials that exhibit unique electron-transfer properties. Our investigators have filed several patents and have also have established five spin-off businesses (3 MSU, 2 UM) and a research center (MT Tech). In addition, this project involved faculty and students at three campuses (MSU, UM, MT Tech) and has a number of under-represented students, including 10 women and 5 Native Americans. In 2006, there was an added emphasis on exporting seminars and speakers via the Internet from UM to Chief Dull Knife Community College, as well as work with the MT Department of Commerce to better educate our faculty regarding establishing small businesses, licensing and patent issues, and SBIR program opportunities

RIP4 inhibits STAT3 signaling to sustain lung adenocarcinoma differentiation.

Loss of epithelial differentiation and extracellular matrix (ECM) remodeling are known to facilitate cancer progression and are associated with poor prognosis in patients with lung cancer. We have identified Receptor-interacting serine/threonine protein kinase 4 (RIP4) as a regulator of tumor differentiation in lung adenocarcinoma (AC). Bioinformatics analyses of human lung AC samples showed that poorly differentiated tumors express low levels of RIP4, whereas high levels are associated with better overall survival. In vitro, lung tumor cells expressing reduced RIP4 levels showed enhanced activation of STAT3 signaling and had a greater ability to invade through collagen. In contrast, overexpression of RIP4 inhibited STAT3 activation, which abrogated interleukin-6-dependent induction of lysyl oxidase, a collagen cross-linking enzyme. In an autochthonous mouse model of lung AC initiated by Kras(G12D) expression with loss of p53, Rip4 knockdown tumors progressed to a poorly differentiated state marked by an increase in Hmga2, reduced Ttf1, and enrichment of genes regulating extracellular remodeling and Jak-Stat signaling. Tail vein injections of cells overexpressing Rip4 showed a reduced potential to invade and form tumors, which was restored by co-expression of Stat3. Altogether, our work has identified that loss of RIP4 enhances STAT3 signaling in lung cancer cells, promoting the expression of ECM remodeling genes and cancer dedifferentiation

Adaptation and Mal-Adaptation to Ambient Hypoxia; Andean, Ethiopian and Himalayan Patterns

The study of the biology of evolution has been confined to laboratories and model organisms. However, controlled laboratory conditions are unlikely to model variations in environments that influence selection in wild populations. Thus, the study of “fitness” for survival and the genetics that influence this are best carried out in the field and in matching environments

Frequency drift in MR spectroscopy at 3T

Purpose: Heating of gradient coils and passive shim components is a common cause of instability in the B-0 field, especially when gradient intensive sequences are used. The aim of the study was to set a benchmark for typical drift encountered during MR spectroscopy (MRS) to assess the need for real-time field-frequency locking on MRI scanners by comparing field drift data from a large number of sites.Method: A standardized protocol was developed for 80 participating sites using 99 3T MR scanners from 3 major vendors. Phantom water signals were acquired before and after an EPI sequence. The protocol consisted of: minimal preparatory imaging; a short pre-fMRI PRESS; a ten-minute fMRI acquisition; and a long post-fMRI PRESS acquisition. Both pre- and post-fMRI PRESS were non-water suppressed. Real-time frequency stabilization/adjustment was switched off when appropriate. Sixty scanners repeated the protocol for a second dataset. In addition, a three-hour post-fMRI MRS acquisition was performed at one site to observe change of gradient temperature and drift rate. Spectral analysis was performed using MATLAB. Frequency drift in pre-fMRI PRESS data were compared with the first 5:20 minutes and the full 30:00 minutes of data after fMRI. Median (interquartile range) drifts were measured and showed in violin plot. Paired t-tests were performed to compare frequency drift pre- and post-fMRI. A simulated in vivo spectrum was generated using FID-A to visualize the effect of the observed frequency drifts. The simulated spectrum was convolved with the frequency trace for the most extreme cases. Impacts of frequency drifts on NAA and GABA were also simulated as a function of linear drift. Data from the repeated protocol were compared with the corresponding first dataset using Pearson's and intraclass correlation coefficients (ICC).Results: Of the data collected from 99 scanners, 4 were excluded due to various reasons. Thus, data from 95 scanners were ultimately analyzed. For the first 5:20 min (64 transients), median (interquartile range) drift was 0.44 (1.29) Hz before fMRI and 0.83 (1.29) Hz after. This increased to 3.15 (4.02) Hz for the full 30 min (360 transients) run. Average drift rates were 0.29 Hz/min before fMRI and 0.43 Hz/min after. Paired t-tests indicated that drift increased after fMRI, as expected (p &lt; 0.05). Simulated spectra convolved with the frequency drift showed that the intensity of the NAA singlet was reduced by up to 26%, 44 % and 18% for GE, Philips and Siemens scanners after fMRI, respectively. ICCs indicated good agreement between datasets acquired on separate days. The single site long acquisition showed drift rate was reduced to 0.03 Hz/min approximately three hours after fMRI.Discussion: This study analyzed frequency drift data from 95 3T MRI scanners. Median levels of drift were relatively low (5-min average under 1 Hz), but the most extreme cases suffered from higher levels of drift. The extent of drift varied across scanners which both linear and nonlinear drifts were observed.</p