Medical Countermeasures for Radiation Induced Health Effects: Reports of an Interagency Panel Session Held at the NASA Human Research Program Investigator's Workshop, January 26, 2017

An Interagency Panel Session organized by the NASA Human Research Program Space Radiation Program Element (SRPE) was held during the NASA Human Research Program (HRP) Investigators Workshop (IWS) in Galveston, Texas on January 26, 2017 to identify complementary research areas that will advance the testing and development of medical countermeasures (MCM) in support of radioprotection and radiation mitigation on the ground and in space. There were several areas of common interest identified among the various participating agencies. This report provides a summary of the topics discussed by each agency along with potential areas of intersection for mutual collaboration opportunities. Common goals included repurposing of pharmaceuticals, neutraceuticals for use as radioprotectors and/or mitigators, low-dose/chronic exposure paradigms, late effects post-radiation exposure, mixed-field exposures of gamma-neutron, performance decrements, and methods to determine individual exposure levels

JUMPING STRATEGIES IN A VOLLEYBALL AND A BALLET SPECIFIC JUMP

INTRODUCTION The performance of a maximal vertical jump fram a static preparatory position (SQJ) or starting with a counter movement (CMJ) implies transformation of rotation about the hip, knee and ankle joints to a maximal translatory movement. Different strategies have been proposed for this transformation. Previously both sequential and simullaneous strategies have been proposed as optimal for maximal vertical jumping (1 & 2). The purpose of this study was to analyze ]umping strategies in a sport and dance specific maximal vertical jump. The hypothesis was that the technical demands of the Jumps would preset the strategy. Six male subjects participated in the study three professional ballet dancers and three elite volleyball players. In the ballet specific jump (BSJ) the legs were outward rotated, one foot was placed in front of and close to the other foot and the upper body kept upright. Three elite volleyball players performed the jump used for the smash (VSJ) including a three step preliminary run up and a farcefull arm swing. Afterwards all six subjects performed SQJ and CMJ. The ]umps were recorded on high speed film (500Hz) combined with registrations trom an AMTI force platform and EMG recordings from the major lower extremity muscles Net joint moments and joint work ware calculated by inverse dynamics. The strategy of the jumps was determined on the basis of angular kinematics and the pattern of nel joint moments of the two dominant joints RESULTS For BSJ the jumping height (h) was 0.22O.28m.The war!< contribution from the knee and ankle joint were 50-70% and 47-63% of the total work respectively while the work at the hip joint showed a negative contribution of 13-17% caused by a net hip flexor moment. Because of the specific ballet position the hip extension took place in the frontal plane and mgluteus maximus could not contribute to the extension. The concentric activity in mrectus femoris could partly explain the hip flexor moment. The knee and ankle joint initiated the extension phase simultaneously and the net joint moments peaked also simultaneously Therefore, the strategy could be defined as simultaneous. For VSJ h was 0.310.45m. The work contribution fram the knee and hip joints were 22-60% and 35-62% of the total work respectively. The hip joint began the extension phase before the body center of mass had reached its lowest position (sn The knee extension began 40-100ms after s.j. The peaks of the net joint moments of the hip and knee showed a similar pattern. Accordingly, the strategy could be defined as sequentiaL The sequential joint extension could partly be explained by the forcefull armswing pressing down and giving negative momentum in the downward phase and by this delaying the knee extension. In SQJ and CMJ h was 0.22-0.36m and 0.33-0AOm. The work contribution from the knee was 64.5%(SE 5.9) and 76.0% (SE 9.2) and from the hip 18.8% (SE 5.8) and 133% (SE 8.7). One ballet dancer and one volleyball player performed SQJ and CMJ with a simultaneous strategy while the otller four subjects used a sequential strategy. CONCLUSION In a maximal vertical jump fram ballet and from volleyball the technical demands preset the jumping strategy. When the subjects were asked to perform SQJ and CMJ the choice of strategy seemed individual and not connected to the training background. REFERENCES (1) Hudson, J.L. (1986). Med Sci. Sports Exerc, 18,242-251 (2) Babbert, M.F. & van lngen Schenau, G.J. (1986). J Biomechanics, 21, 249•26

Bodies, building and bricks: Women architects and builders in eight eco-communities in Argentina, Britain, Spain, Thailand and USA

Eco-building is a male domain where men are presumed to be better builders and designers, more men than women build and women find their design ideas and contributions to eco-building are belittled. This article suggests that a focus on bodies, embodiment and the ‘doing’ of building is a potentially productive way to move beyond current gender discrimination. This article makes three key interventions using empirical material from eight case studies of eco-communities in Britain, Thailand, Spain, the USA and Argentina. First, it uses a focus on eco-communities to illustrate the enduring persistence of gender divisions in architecture and building. Second, by using multi-site examples of eco-communities from diverse countries this article finds more commonalities than differences in gender discrimination across cultures and nationalities. Third, it outlines three spaces of opportunity through which more gender-neutral approaches are being developed in eco-building: (1) in challenging the need for ‘strong’ bodies, (2) by practising more embodied ways of building and (3) by making visible women's bodies in building. The ‘doing’ and manual aspect of eco-building is unfamiliar for many (not just women) and interviewees commented on the need to (re)learn how to be practical and to understand the physical possibilities (and limitations) of their bodies

Enzyme prodrug therapy achieves site-specific, personalized physiological responses to the locally produced nitric oxide

Nitric oxide (NO) is a highly potent but short-lived endogenous radical with a wide spectrum of physiological activities. In this work, we developed an enzymatic approach to the site-specific synthesis of NO mediated by biocatalytic surface coatings. Multilayered polyelectrolyte films were optimized as host compartments for the immobilized β-galactosidase (β-Gal) enzyme through a screen of eight polycations and eight polyanions. The lead composition was used to achieve localized production of NO through the addition of β-Gal–NONOate, a prodrug that releases NO following enzymatic bioconversion. The resulting coatings afforded physiologically relevant flux of NO matching that of the healthy human endothelium. The antiproliferative effect due to the synthesized NO in cell culture was site-specific: within a multiwell dish with freely shared media and nutrients, a 10-fold inhibition of cell growth was achieved on top of the biocatalytic coatings compared to the immediately adjacent enzyme-free microwells. The physiological effect of NO produced via the enzyme prodrug therapy was validated ex vivo in isolated arteries through the measurement of vasodilation. Biocatalytic coatings were deposited on wires produced using alloys used in clinical practice and successfully mediated a NONOate concentration-dependent vasodilation in the small arteries of rats. The results of this study present an exciting opportunity to manufacture implantable biomaterials with physiological responses controlled to the desired level for personalized treatment

Free fatty acids link metabolism and regulation of the insulin-sensitizing fibroblast growth factor-21

OBJECTIVE—Fibroblast growth factor (FGF)-21 improves insulin sensitivity and lipid metabolism in obese or diabetic animal models, while human studies revealed increased FGF-21 levels in obesity and type 2 diabetes. Given that FGF-21 has been suggested to be a peroxisome proliferator–activator receptor (PPAR) –dependent regulator of fasting metabolism, we hypothesized that free fatty acids (FFAs), natural agonists of PPAR, might modify FGF-21 levels. RESEARCH DESIGN AND METHODS—The effect of fatty acids on FGF-21 was investigated in vitro in HepG2 cells. Within a randomized controlled trial, the effects of elevated FFAs were studied in 21 healthy subjects (13 women and 8 men). Within a clinical trial including 17 individuals, the effect of insulin was analyzed using an hyperinsulinemic-euglycemic clamp and the effect of PPAR activation was studied subsequently in a rosiglitazone treatment trial over 8 weeks. RESULTS—Oleate and linoleate increased FGF-21 expression and secretion in a PPAR-dependent fashion, as demonstrated by small-interfering RNA–induced PPAR knockdown, while palmitate had no effect. In vivo, lipid infusion induced an increase of circulating FGF-21 in humans, and a strong correlation between the change in FGF-21 levels and the change in FFAs was observed. An artificial hyperinsulinemia, which was induced to delineate the potential interaction between elevated FFAs and hyperinsulinemia, revealed that hyperinsulinemia also increased FGF-21 levels in vivo, while rosiglitazone treatment had no effect. CONCLUSIONS—The results presented here offer a mechanism explaining the induction of the metabolic regulator FGF-21 in the fasting situation but also in type 2 diabetes and obesity